Detection Of Tetracycline Research Articles

Dual-response ratiometric fluorescent probe for visual and colorimetric detection of tetracycline

Dual-response ratiometric fluorescent probe for visual and colorimetric detection of tetracycline

High fluorescent nitrogen − doped carbon dots derived from Sanghuangporus lonicericola for detecting tetracyclines in aquaculture water and rat serum samples

Tetracyclines (TCs) are a kind of broad − spectrum and effective antibiotics that have been widely used in veterinary and human medicine, which may pose serious risk to human health if they are accumulated in human body. The fluorescence method using nanomaterials as probes has been gradually used for the detection of TCs because of its simple operation, high sensitivity and recognition ability. Herein, a novel nitrogen doped carbon dots (N − CDs) derived from Sanghuangporus lonicericola was synthesized by hydrothermal method. The N − CDs exhibited bright blue fluorescence, high quantum yield (QY) of 60.99 %, and excellent photostability, which was employed as a fluorescent probe for selective detection of TCs, including oxytetracycline (OXY), doxycycline (DOX), tetracycline (TCY) and minocycline (MNO). The fluorescence intensity of the N − CDs decreased linearly with the increasing concentration of OXY, DOX, TCY and MNO, and their concentrations were in the range of 0.6 – 73.3 μM, 0 – 95.2 μM, 6.5 – 72.3 μM and 10 – 52.6 μM, respectively. As for practical application, the as − prepared N − CDs could not only be effectively applied for determination of TCs in aquaculture water and rat serum samples with low relative standard deviation (RSD) and good recoveries, but also track the concentration change of TCs in rats blood model over time. Towards the fluorescence detection mechanism, the results of UV–vis absorption spectra and fluorescence lifetimes proved that the fluorescent quenching of N − CDs occurred from internal filtration effect (IFE) in the presence of TCs. This work provides guidance for the development of new TCs sensors based on fluorescent nanomaterials, and has a good application prospect in the quality control of agriculture, food and pharmaceutical production processes.

Secondary Validation of a Liquid Scintillation Counter-method for Residues of Tetracyclines, Beta-lactams, and Sulfonamides in Seafood/Aquaculture Products

This study was carried out to validate the liquid scintillation counter method (Charm II) for the detection of tetracyclines, beta-lactams, and sulfonamides (Sulfa drugs) in a range of Aquaculture Products. This method of validation followed primary validation performed in Belgium and was therefore transferred to Nigeria but further validation was required, and this was performed according to the European Commission Decision 2002/657/EC. Method performance was based on the detection capability (CCβ), specificity (cross-reactivity), robustness, repeatability, and reproducibility for the detection of antimicrobial residues. Seafood and aquaculture samples used for the validation process included tilapia (Oreochromis niloctus), catfish (Siluriformes), African threadfin (Galeoides decadactylus), common carp (Cyprinus carpio), and shrimps (penaeidae). These were spiked with varying concentrations of tetracyclines, beta-lactams, and sulfonamides standards to determine the validation parameters. Results of the validation showed tetracyclines had detection capabilities of 50 µg/kg, while beta-lactams and sulphonamides had detection capabilities of 25 µg/kg. The relative standard deviation for both repeatability and reproducibility studies ranged between 1.36% and 10.50%. Results of this study are suitable and comparable to the initial validation reports from the primary validation ofCharm II tests forthedetection ofantimicrobial residues inarange ofaquaculture fish conducted in Belgium. The results also prove the specificity, ruggedness, and reliability of the radio receptor assay tests for detection of the various antimicrobials in aquaculture products. This could be used in seafood/aquaculture products monitoring in Nigeria.

Nanoarchitectonics of electrochemical aptasensor based on electrospun carbon nanofibers and gold nanoparticles for tetracycline detection in chicken ham

Nanoarchitectonics of electrochemical aptasensor based on electrospun carbon nanofibers and gold nanoparticles for tetracycline detection in chicken ham

A simple paper-based nickel nanocluster-europium mixed ratio fluorescent probe for rapid visual sensing of tetracyclines

In this work, a ratiometric fluorometric sensor based on nickel nanoclusters (NiNCs)-europium complex (NiNCs-Eu3+) was constructed for the highly selectivity detection of tetracyclines (TCs) in water samples. In the presence of TCs, the blue fluorescence of the sensor NiNCs-Eu3+ was quenched at 430 nm and the characteristic red fluorescence of Eu3+-TCs appeared at 620 nm because of the combined help of inner filter effect (IFE) and antenna effect. Under the optimized conditions (100 mM Eu3+ (100 µL); temperature (25℃); reaction time (10 min), HEPES buffer solution (pH = 7.0)), the sensor offered a wide detection range of tetracycline (TC) and oxytetracycline (OTC) from 0.1 to 50 μM with the detection limit (LOD) of 25 nM and 21 nM, respectively. Moreover, the sensor was able to detect of TC and OTC in tap and lake water with high recovery rate (89.10%-97.60%). In addition, the portable paper-based sensor was constructed using filter paper embedded with NiNCs-Eu3+. The distinct fluorescent color of the paper-based sensor varied from bright blue to red against different concentrations of TC and OTC. These above findings demonstrated the potential for wide application of as-prepared ratio metric fluorescence sensor for visual detection of TCs in water samples.

Cover Feature: An Origami Paper‐Based Analytical Device Coupled with Fast‐Responding Functional RNA Superstructures for Tetracycline Detection (Anal. Sens. 3/2023)

Cover Feature: An Origami Paper‐Based Analytical Device Coupled with Fast‐Responding Functional RNA Superstructures for Tetracycline Detection (Anal. Sens. 3/2023)

A luminescent probe based on a Zn(II) coordination polymer for efficient detection of tetracycline, Cr2O72− anions

A luminescent probe based on a Zn(II) coordination polymer for efficient detection of tetracycline, Cr2O72− anions

A ratiometric fluorescence sensor for tetracycline detection based on two fluorophores derived from Partridge tea

Blue fluorescent carbon dots (PCDs) were prepared by hydrothermal method with Partridge tea. The ethanol extract of Partridge tea (PEE) was found to emit red fluorescence. Thus, a novel ratiometric sensor was constructed by simply mixing the two fluorophores derived from Partridge tea. The presence of tetracycline (TET) at lower concentrations enhanced the emission peak at 508nm of PCDs and had a negligible effect on the emission peak at 680nm of PEE. TET at higher concentrations led to quenching both the fluorescence of PCDs and PEE via inner filter effect and fluorescence resonance energy transfer, separately. Good linearities for the detection of TET were obtained in the ranges 0.67 to 15.00μM and 33.33 to 266.67μM, with limit of detection of 0.095μM. The sensor was successfully applied to detect TET in lake water and milk samples with good recoveries ranging from 93.27 ± 4.04% to 107.30 ± 6.16%. This study provided a simple, selective, sensitive, rapid, and environmentally friendly method of monitoring TET residues in the environment and food.

A label-free fluorescent biosensor based on specific aptamer-templated silver nanoclusters for the detection of tetracycline

Tetracycline (TET) is a broad-spectrum antibiotic commonly used in the treatment of animals. TET residues in food inevitably threaten human health. High-performance analytical techniques for TET detection are required in food quality assessment. The objective of this study was to establish a label-free fluorescent biosensor for TET detection using specific aptamer-templated silver nanoclusters (AgNCs). An aptamer with a high specific binding ability to TET was used to synthesize a novel DNA-templated AgNCs (DNA-AgNCs). When TET is present, the aptamer’s conformation switched from an antiparallel G-quadruplex to a hairpin structure, altering the connection between AgNCs and the aptamer. Following the transformation of AgNCs into large sized silver nanoparticles (AgNPs), a fluorescence decrease was detected. When used to detect TET in milk, the proposed biosensor displayed high sensitivity and selectivity, with a limit of detection of 11.46 ng/mL, a linear range of 20 ng/mL−10 g/mL, and good recoveries of 97.7–114.6% under optimized conditions. These results demonstrate that the proposed biosensor was successfully used to determine TET quantitatively in food samples, suggesting that our method provides an efficient and novel reference for detecting antibiotics in food while expanding the application of DNA-AgNCs in related fields.

Dual-responsive ratiometric fluorescent sensor for tetracyclines detection based on europium-decorated copper nanoclusters

Development of accurate and efficient TCs residue analysis methods is of great significance for the protection of environment, food safety and public health. Herein, a dual-responsive ratiometric fluorescence sensor being capable of simple and sensitive detection of tetracycline (TC) was presented, which was constructed by immobilizing europium ions (Eu3+) onto the mercaptopropionic acid stabilized copper nanoclusters (MPA-Cu NCs). In the presence of TC, the red fluorescence of Eu3+ was enhanced through antenna effect (AE), while the green fluorescence of MPA-Cu NCs was quenched through internal filter effect (IFE), leading to an obvious fluorescence color evolution from green to red for the probe solution. In addition to successful design of a smartphone-assisted colorimetric analysis platform for portable detection, a logic gate device capable of intelligently monitoring TC concentration is also designed.

A Zn(II)-Metal-Organic Framework Based on 4-(4-Carboxy phenoxy) Phthalate Acid as Luminescent Sensor for Detection of Acetone and Tetracycline.

As hazardous environmental pollutants, residual tetracycline (TC) and acetone are harmful to the ecosystem. Therefore, it is necessary to detect the presence of these pollutants in the environment. In this work, using Zn (II) salt, 4-(4-carboxy phenoxy) phthalic acid (H3L), and 3,5-bis(1-imidazolyl) pyridine (BMP), a new metal-organic framework (Zn-MOF) known as [Zn3(BMP)2L2(H2O)4]·2H2O was synthesized using a one-pot hydrothermal method. The Zn-MOF has a three-dimensional framework based on the [Zn1N2O2] and [Zn2N2O4] nodes linked by a tridentate bridge BMP ligand and an L ligand with the μ1:η1η0/μ1:η1η0/μ0:η0η0 coordination mode. There were two kinds of left- and right-handed helix chains, Zn1-BMP and Zn1-BMP-Zn1-L. The complex was stable in aqueous solutions with pH values of 4-10. The Zn-MOF exhibited a strong emission band centered at 385 nm owing to the π*→π electron transition of the ligand. It showed high luminescence in some common organic solvents as well as in the aqueous solutions of pH 4-10. Interestingly, TC and acetone effectively quenched the luminescence of the Zn-MOF in aqueous solution and enabled the Zn-MOF to be used as a sensor to detect TC and acetone. The detection limits of TC and acetone were observed to be 3.34 µM and 0.1597%, respectively. Even in acidic (pH = 4) and alkaline (pH = 10) conditions, the Zn-MOF showed a stable luminescence sensing capability to detect TC. Luminescence sensing of the Zn-MOF for TC in urine and aquaculture wastewater systems was not affected by the interfering agent. Furthermore, the mechanism of sensing TC was investigated in this study. Fluorescence resonance energy transfer and photoinduced electron transfer were found to be the possible quenching mechanisms via UV-Vis absorption spectra/the excitation spectra measurements and DFT calculations.

Highly sensitive detection of tetracycline by electrochemical molecular imprinting

Highly sensitive detection of tetracycline by electrochemical molecular imprinting

A fluorescent dual-emissions UiO-66-NH2@TpTt-COF core-shell composite for sensitive and optosmart sensing of tetracycline

Tetracycline residues are seriously hazardous to environment, it’s urgent to develop sensors to detect tetracycline for environmental monitoring. A fluorescence sensor based on dual-emissions UiO-66-NH2@TpTt-COF (donate as UNT) composite is constructed by an interfacial growth method for detecting tetracycline. The import of UiO-66-NH2 attenuates the aggregation-caused quenching (ACQ) of TpTt-COF, which endows UNT emitting strong dual-emissions of 452 nm and 575 nm. Tetracycline is recognized by UNT through π-π stacking and interacts with triazine N and deprotonated O of UNT through intermolecular hydrogen bonds. These interactions impede the excited state intramolecular proton transfer (ESIPT) and intramolecular charge transfer (ICT), and promote the photoinduced electron transfer (PET), inducing synchronous fluorescent quenching of the dual-emissions of UNT. The synchronous response of UNT enhances the specificity and displays excellent sensitivity toward tetracycline with a detection limit of 7.4 nmol/L. UNT is applied for the detection of tetracycline in soil and river water with recoveries of 92.96–98.44% and 88.58–104.31%, respectively. An optosmart sensing system has also been developed to enable visual detection of tetracycline. This work provides a novel synchronous response sensor for antibiotic detection and opens a new path for core-shell MOF@COF composites in the field of environmental monitoring.

Highly sensitive detection of tetracycline and Fe3+ and for visualizable sensing application based on a water-stable luminescent Tb-MOF

Excessive use of tetracycline (TC) or Fe3+ ions aggravates environmental pollution and threatens human health seriously. A water-stable 3D Tb-MOF ([Tb2(L)3]·2H2O, 1-Tb) is reported by employing H2L (5-(pyrazin-2-yl)isophthalic acid) as bridging ligands. The MOF is a dual-functional luminescence sensor for the detection of TC and Fe3+ in aqueous media with good sensitivity and selectivity even in the presence of common pollutants. Moreover, a low limit of detection and a high quenching constant obtained from the linear range for TC and Fe3+ detection are 0.51 μM, 1.47 × 104 M−1 and 0.78 μM, 7.73 × 103 M−1, respectively. The possible quenching mechanism is discussed by PXRD, UV–vis absorption spectroscopy, luminescence lifetime, SEM, and XPS in detail. The results showed that the high quenching efficiency is attributed to the competitive absorption of excitation energy mechanism. Furthermore, the MOF was successfully fabricated as a strip-shaped film (1-Tb-PVDF) with a visualized luminescence response under irradiation with a UV lamp, providing a simple tool for the rapid detection of TC and Fe3+ in aqueous solutions. This work provides a new dual-functional material for detecting the TC and Fe3+ in water.

The synthesis of N-doped carbon dots for visual differentiating and detection of tetracyclines.

N-doped carbon dots (N-CDs) were synthesized from L-glutamine and triethanolamine via one-step hydrothermal method. The N-CDs emitting blue fluorescence have selective response to tetracyclines (TCs), and can be used as a fluorescent probe to realize the quantitative detection and qualitative analysis of TCs. A method for the determination of TCs by the N-CDs in actual samples has been successfully established. The recovery rate was maintained at 97.50-105.60%, and the relative standard deviation (RSD) was below 3%. Meanwhile, TCs can be visually distinguished using filter paper by the different fluorescence colors (light green, dark blue and yellow-green) of the N-CDs/TCs system under ultraviolet (UV) light. This study provides a relatively simple method to detect and identify TCs.

An electronic tongue based on conjugated polymers for the discrimination and quantitative detection of tetracyclines.

A fluorescent sensor array has been developed based on conjugated polymers (CPs) having six different skeletons for the detection of tetracyclines (TCs), which are known as environmental pollutants. CPs were synthesized from confined nanoreactors in a controlled manner. The fluorescent response occurs through the fluorescence resonance energy transfer (FRET) effect. By utilizing linear discriminant analysis (LDA), effective differentiation of TCs was accomplished with a very low detection concentration (66 nM). Moreover, the sensor array exhibited a highly sensitive ability to quantitatively distinguish different concentrations of TCs. Finally, the sensor array's potential for detecting TCs in aqueous solutions has been successfully demonstrated, widening its applications in practical environments. With simple preparation process, a low cost of detection and high sensitivity, the experimental results indicate that the CP-based sensor array is a promising platform for the sensitive and quantitative detection of TCs, and provides a good reference for future scientific research.

A portable test strip fabricated of luminescent lanthanide-functionalized metal-organic frameworks for rapid and visual detection of tetracycline antibiotics.

Tetracycline antibiotics (TCs) are commonly used antibiotics in the treatment of infections, but their overuse has a negative impact on human health and ecosystems. Thus, the development of a facile and on-site visualization method for TC detection is necessary. Here, we propose the potential of using lanthanide-functionalized metal-organic framework (MOF) composites (Ag+/Tb3+@UiO-66-(COOH)2, ATUC) as a probe for the rapid detection of tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC), and doxycycline (DOX) residues, in which UiO-66-(COOH)2 (UC) could be utilized to provide an interaction microenvironment, Tb3+ as recognition units and Ag+ as a fluorescence enhancer. Upon exposure to TCs, significant luminescence quenching of ATUC excited at 255 nm was observed due to the inner filter effect (IFE) and photo-induced electron transfer (PET), and the established strategy has a detection limit (LOD) of 11.0, 20.1, 9.1, and 22.5 nM for TC, CTC, OTC, and DOX, respectively. More importantly, given its portability and conspicuous luminescence color gradation variation, a portable test strip based on ATUC was manufactured and the results could be distinguished immediately by the naked eye and smartphone analysis, allowing for on-site rapid quantitative assay of TCs, not only in the laboratory but also in a point-of-care setting.

Ratiometric Fluorescence Detection of Tetracycline in Milk Based on Inner Filter Effect and Antenna Effect for Non-Conjugated Polymer Point and Eu3+

Ratiometric Fluorescence Detection of Tetracycline in Milk Based on Inner Filter Effect and Antenna Effect for Non-Conjugated Polymer Point and Eu3+

TPN-COF@Fe-MIL-100 composite used as an electrochemical aptasensor for detection of trace tetracycline residues.

In this work, a metal-organic framework@covalent organic framework composite (TPN-COF@Fe-MIL-100) was prepared and used as a sensing material to construct an aptasensor for trace detection of tetracycline (TET). The TPN-COF@Fe-MIL-100 integrates a large surface area, porous structure, excellent electrochemical activity, rich chemical functionality, and strong bioaffinity for aptamers, providing abundant active sites to effectively anchor aptamer strands. As a result, the TPN-COF@Fe-MIL-100-based aptasensor shows high sensitivity for detecting TET via specific recognition between aptamer and TET to form G-quadruplex. An ultralow detection limit of 1.227 fg mL-1 is deduced from the electrochemical impedance spectroscopy within a wide linear range of 0.01-10000 pg mL-1 for TET. The TPN-COF@Fe-MIL-100-based aptasensor also exhibits good selectivity, reproducibility, stability, regenerability, and applicability for a real milk sample. Therefore, the TPN-COF@Fe-MIL-100-based aptasensor will be promising for detecting trace harmful antibiotics residues for food safety.

A post-synthetically modified Eu@Zn-MOF for ratiometric fluorescence detection of tetracycline in tap water

A Eu3+ functionalized zinc metal–organic framework (Eu@Zn-MOF) has been prepared by the post-synthetic modification method and innovatively used to construct a ratiometric fluorescent probe for tetracycline detection.