Detection Of Mast Cells Research Articles

MAST CELLS AND VESSELS OF THE CHOROID OF ALBINO GUINEA PIGS DURING THE UVEAL ANAPHYLACTIC REACTION.

Mast cell degranulation and liberation of vasoactive substances during anaphylactic reaction were parallel in guinea pigs,¹rats,²and mice.³ It was also shown that connective tissue and choroid mast cells are both degranulated by compound 48/80.^4-7 In the present study the alterations of vessels and mast cells of the posterior uvea in local anaphylactic reactions are reported. <h3>Materials and Methods</h3><h3>Antigens.</h3> —(<i>a</i>) Human gamma globulin (Lederle, C-583); (<i>b</i>) Human albumin (Behringwerke, Op. Nr. 1916-D, 358/E 297). <h3>Antiserum.</h3> —Rabbit antihuman globulin containing 884μg antibody nitrogen per milliliter (Ab N). <h3>Dye.</h3> —Colloidal carbon (Gunther Wagner—C 11/ 1431a), suspended according to Biozzi et al⁸and injected intravenously (8 mg/100 gm body weight). <h3>Diluent.</h3> —All reagents except the carbon were dissolved in 0.15 M NaCl. <h3>Animals.</h3> —Male albino guinea pigs weighing 250 to 300 gm (uveal pigment in pigmented animals makes it difficult to detect mast cells). <h3>Experiment</h3>

Mast cell response to cestode infection.

SummaryMast cells have been found in the cyst wall of a larval cestode parasite (Hydatigena taeniaeformis). The mast cells were present in the outer 2 layers of the cyst wall. This indicates that the entire cyst wall is of host-inflammatory origin and not derived from parasite tissue nor solely from modified host-liver cells. It is suggested that detection of mast cells as host-mesenchymal cellular units may aid in determining origins of cysts produced by other parasites.