Detection Of L-histidine Research Articles

Fluorescence Sensors for the Detection of L-Histidine Based on Silver Nanoclusters Modulated by Copper Ions.

In this study, Cu2+ modulated silver nanoclusters were constructed for the turn-on, label-free detection of L-histidine. Six Ag NCs protected by oligonucleotides (DNA-Ag NCs) were tested in a series of experiments. Finally, A-DAN-Ag NCs were chosen as the best candidate due to their excellent fluorescent properties. The fluorescence of A-DAN-Ag NCs was quenched using Cu2+ through energy or electron transfer. However, quenched fluorescence could be restored dramatically in the presence of L-histidine due to Cu2+ liberation from A-DAN-Ag NCs and because of the chelation between the imidazole group of L-histidine and Cu2+. The proposed sensor exhibited high selectivity towards L-histidine over other amino acids, with a limit of detection (LOD) of 0.096 μM ranging from 0 to 8 μM. The proposed sensor succeeded in detecting L-histidine in diluted human urine. Therefore, the sensor has promising practical applications in biological systems.

A DMSO-assisted iridium(III) complex as a luminescent "turn-on" sensor for selective detection of L-histidine and bacterial imaging.

Histidine (His) is a semi-essential amino acid and a unique key neurotransmitter involved in numerous physiological processes. An excessive or deficient amount of His in the body can lead to various related diseases. However, since the chemical structures of L-His and its metabolites (such as histamine (Ha), imidazole-4-acetate (ImA), etc.) are very similar, simple and efficient selective detection of L-His and its related metabolites is of great importance but remains a great challenge. Herein, we successfully designed and synthesized a DMSO-assisted iridium(III) complex (Ir1-DMSO), which can be applied as a "turn-on" photoluminescence (PL) probe for the selective detection and quantification of L-His/Ha. More importantly, Ir1-DMSO exhibited good sensitivity, high selectivity, and anti-interference capability for L-His/Ha/His-containing proteins, which is advantageous due to its simple fabrication and low technical demands. This was attributed to the reaction of Ir1-DMSO with imidazole and amino groups of L-His/Ha. Furthermore, we show the utility of Ir1-DMSO as a PL imaging agent in cultures of E. coli and S. aureus. Considering its diversity of composition and structural flexibility, it can be extended to other solvents and Ir-ligand complexes for various analyses based on specific molecular recognition sensing platforms.

A near-infrared fluorescence capillary imprinted sensor for chiral recognition and sensitive detection of l-histidine

This work firstly developed a near-infrared fluorescence capillary imprinted sensor with high selectivity and sensitivity for the chiral recognition of l-histidine. The near-infrared fluorescence imprinted polymer prepared by sol-gel method using CdTe quantum dots as the near-infrared fluorescence source was self-sucked into activated capillary to form the fluorescence imprinted capillary. The fluorescence imprinted capillary sensor had higher fluorescence response efficiency. With the superior stability, reproducibility and reusability, the fluorescence capillary imprinted sensor displayed higher selectivity toward l-histidine. Under the optimal conditions, the fluorescence intensity (λex = 370 nm, λem = 695 nm) of the fluorescence capillary imprinted sensor was enhanced in proportion to l-histidine concentration ranged from 0.1 pM to 1.8 pM with a limit of detection of 0.08 pM. Spiking experiment showed that the fluorescence capillary imprinted sensor was successfully used for determination of l-histidine in human urine and serum with the recoveries of 99.6–102.7%. The near-infrared fluorescence capillary imprinted sensor possessed significantly potential for high sensitive detection of l-histidine, which realized trace-level analysis for micro-volume sample and provided promising utility for green chemical.

Single nanoparticle identification coupled with auto-identify algorithm for rapid and accurate detection of L-histidine

In this work, an auto-identify sensor was constructed for rapid and high-precision detection of L-histidine. The proposed strategy is based on the auto-identify algorithm and the aggregation of alkynyl and azide functionalized gold nanoparticles induced by the Cu+ catalyzed azides and alkynes cycloaddition (CuAAC) reaction. Specially, the color of scattering light spots for the aggregated gold nanoparticle (AuNPs) caused by CuAAC reaction was quite different from that of the monomers. However, L-histidine can bind to Cu2+ and inhibits the production of Cu+, hence preventing the aggregation of AuNPs. Therefore, there is a distinct change of color as the addition of L-histidine under dark-field microscopy. Then, L-histidine can be quantitatively detected by combining the color change with the Meanshift algorithm accurately and automatically. Such proposed method has been successfully applied for the detection of L-histidine in serum sample with satisfying result.

A Turn-on Fluorescent Sensor Based on Copper-Based Metal-Organic Frameworks for Sensitive Detection of L-Histidine

As an important amino acid in the body, L-histidine participates in various physiological processes. Therefore, it is of great necessity to establish a rapid and effective method for L-histidine sensing, especially in blood and urine. In this work, a fluorescence sensor for detection of L-histidine was successfully developed using Cu-MOFs/Fe[Formula: see text] sensor. Cu-MOFs with blue emission served as the identification unit and signal source, and Fe[Formula: see text] played the role of “quencher” in this system. Fe[Formula: see text] induced the fluorescence quenching of Cu-MOFs to form Cu-MOFs/Fe[Formula: see text] sensor due to the effect of photoinduced electron transfer (PET). After the addition of L-histidine, Fe[Formula: see text] was captured by L-histidine to form L-histidine-Fe[Formula: see text] complex, which inhibited the PET effect from Cu-MOFs to Fe[Formula: see text], resulting in the fluorescence recovery of Cu-MOFs. With such a design, L-histidine detection was realized with a wide linear range from 1[Formula: see text][Formula: see text]M to 190[Formula: see text][Formula: see text]M. The limit of detection (LOD) was calculated as low as 0.156[Formula: see text][Formula: see text]M. In addition, successful detection of L-histidine in blood serum with high selectivity and sensitivity indicated the real applications and potential diagnostic value of Cu-MOFs/Fe[Formula: see text] system.

Preparation of a portable calorimetry kit and one-step spectrophotometric nanomolar level detection of l-Histidine in serum and urine samples using sebacic acid capped silver nanoparticles

Abstract This work describes the synthesis of sebacic acid capped silver nanoparticles (SA-AgNPs) by the wet chemical method for the detection of l -Histidine in blood serum and urine samples. The synthesized SA-AgNPs were characterized by several techniques. The obtained particles were spherical in shape and the average diameter was calculated by HR-TEM images to be 11 nm. Moreover, the synthesized SA-AgNPs were stable for more than 3 months in solution. The synthesized SA-AgNPs showed the surface plasmon resonance (SPR) band at 420 nm. Interestingly, after the addition of 18.1 μM of l -Histidine into the SA-AgNPs solution, the SPR band absorbance decreased and the colour of the solution changed from brown to colourless. Based on the changes in the SPR band, we calculated the lowest detection limit to be 122 nM. The observed colour shift and spectral changes were due to the aggregation of SA-AgNPs induced by l -Histidine via hydrogen bonding interaction. Additionally, a possible mechanism is discussed. Furthermore, a 268-fold excess of common interferences did not interfere with the detection of 18.1 μM of l -Histidine. We successfully applied the SA-AgNPs probe for the detection of l -Histidine in blood serum and urine samples. Further, we validated our system with the HPLC technique. Importantly, we prepared a portable smartphone aided paper-based kit for on-site monitoring of l -Histidine. We strongly hope that this facile synthesis of SA-AgNPs and the portable paper-based kit will represent a step forward in the field of biosensing and materials science.

Dye/metal ion-based chemosensing ensemble towards l-histidine and l-lysine determination in water via different optical responses.

In this study, a novel simple type of label-free, ultra-sensitive, and highly selective UV-Vis absorption and naked-eye detection of histidine (His) and lysine (Lys) using a dye/metal ion ensemble is developed. The outcoming high sensitivity and selectivity for histidine and lysine were attained by changing the metal ions. The indicator is released due to its displacement from the murexide (Mure)/Cu2+ complex by histidine and the change in absorbance may be due to the further complexation of lysine with the additional coordination sites present in the zinc atom of Mure/Zn2+ complex. The label-free chemosensor provided sensitive and selective detection of l-histidine and l-lysine with detection limits of 9.1 and 9.4nmolL-1, respectively. The protocol especially offers high selectivity for the determination of His and Lys among amino acids found in human urine samples. Furthermore, INHIBIT and NAND molecular logic gates were obtained using chemical inputs and UV-Vis absorbance signal output.

Dual-emission CdTe quantum dot@ZIF-365 ratiometric fluorescent sensor and application for highly sensitive detection of l-histidine and Cu2+

l-histidine acts as a semi-essential amino acid, which is medically used in the treatment of gastric ulcer, anemia, allergies. However, the overuse of l-histidine will result in terrible damage to heart disease, slow growth of animals and water pollution in the environment. In addition, Cu2+ pollution is common environmental pollution in the industry. It has the characteristics of high accumulation, migration, and persistence. Given this, through the post-synthesis strategy, CdTe quantum dots (QDs) were the first time to introduce into zeolitic imidazolate framework-ZIF-365 to synthesis dual-emission hybrid material CdTe@ZIF-365 with high quantum yield. TEM mappings and N2 absorption tests are applied to confirm the combination mode between CdTe quantum dots and ZIF-365. It should be noted that CdTe@ZIF-365 can be successfully utilized as a bi-functional ratiometric sensor for highly sensitive discrimination of l-histidine and Cu2+. Firstly, CdTe@ZIF-365 is applied to a fluorescent ratiometric sensor for Cu2+ with high sensitivity (the Ksv value is 2.7417✕107 [M−1]) and selectivity in the mixed cation ions' solution. On the other hand, CdTe@ZIF-365 also behaved as the first example for an excellent ratiometric fluorescent senor for l-histidine with high sensitivity (the Ksv value is 6.0507✕108 [M−1]) and selectivity in the mixed amino acids’ solutions.

Quartz crystal microbalance based histidine sensor

Herein, quartz crystal microbalance (QCM) biosensor is prepared for the detection of l-histidine by attachment of l-histidine imprinted poly(EGDMA-MAH/Cu(II)) nanoparticles on QCM electrode. The imprinted nanoparticles with the size of 86.43 nm were synthesized via miniemulsion polymerization reaction. Prepared QCM sensors were characterized with ellipsometer, contact angle measurements and FTIR. The thickness measurements demonstrated that the particle thin films were almost monolayer. l-histidine solutions with a concentration range between 6.44 μM and 225.6 μM were introduced to QCM system to determine the adsorption kinetics. Selectivity of the l-histidine imprinted nanoparticles were examined using d-histidine and l-tryptophan as competitor molecules. l-histidine imprinted QCM biosensors was also used for RNAase, lysozyme, cytochrome-C and BSA to investigate the competitive adsorption of surface histidine exposed proteins.

Molecularly imprinted photonic hydrogel sensor for optical detection of L-histidine.

A molecularly imprinted photonic hydrogel (MIPH) is described for the optical determination of L-histidine (L-His). The inverse opal structure of MIPH was obtained by placing silica particles (230nm) in molecularly imprinted polymer on a glass slide. After being fully etched by hydrofluoric acid, this inverse opal structure brings about a high specific surface and plentiful binding sites for L-His. If L-His is absorbed by the modified MIPH, its average effective refraction coefficient is increased. This causes the Bragg diffraction peak to be red-shifted by about 34nm as the concentration of L-His increases from 0 to 100nM. Much smaller diffraction peak shifts are obtained for other amino acids. The detection limit of this method is 10 pM. The response time towards L-His is as short as 60s. In addition, the sensor can be recovered by treatment with 0.1M acetic acid/methanol. It was applied to the determination of L-His in drinks sample. Graphical abstract After absorbing L-histidine, the average effective refractive index of this molecularly imprinted photonic hydrogel (MIPH) is increased, and the Bragg diffraction peak is shifted. The shift of the diffraction peak can be used for the detection of L-His.

Colorimetric detection of L-histidine based on the target-triggered self-cleavage of swing-structured DNA duplex-induced aggregation of gold nanoparticles

A rapid, highly sensitive and selective colorimetric assay is presentedfor visually detecting L-histidine. It is based on L-histidine-triggered self-cleavage of DNA duplex-induced gold nanoparticle (AuNP) aggregation. The citrate-capped AuNPs easily aggregate in a high concentration of salt environment. However, in the presence of L-histidine aptamers (DNA1 and DNA2), the partial strands of DNA1 and DNA2 hybridize to form a DNA duplex with a swing structure. The swing-like DNA duplexes are adsorbed on the surface of AuNPs to improve the stability of AuNPs, and theAuNPs also are better dispersed in high-salt media. When L-histidine is added to the solutions, it catalyzes the self-cleavage of DNA1 to form many single-stranded DNA (ssDNA) fragments. These ssDNA segments areadsorbed on theAuNPs and weaken the stability of AuNPs. Hence, the AuNPs aggregate in high-salt environment, and thisresults in a red-to-blue color change. Under the optimized conditions, L-histidine can be determinedwith a limit of detection of 3.6nM. In addition, the sensor was successfully applied to the determination ofL-histidine in spiked serum samples. Graphical abstract Schematic of a rapid and homogeneous colorimetric L-histidine assay. It combines L-histidine-triggered self-cleavage of the swing-like DNA duplexes and self-cleavage of DNA-induced AuNP aggregation.

A novel label-free colorimetric detection of l-histidine using Cu2+-modulated G-quadruplex-based DNAzymes

We proposed a colorimetric method for l-histidine detection based on Cu2+-mediated DNAzyme and G-quadruplex-hemin complex catalyzed oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). In this system, after the addition of l-histidine, the formation of G-quadruplex-hemin complex will be disturbed, thus the colorimetric signal intensity conversely corresponds to the concentration of histidine. In this assay, a lower detection limit of l-histidine (50 nM) is addressed comparing to previously reported colorimetric methods. The cost is extremely low as the proposed design is both label-free and enzyme-free. All the more vitally, the colorimetric detection procedure is substantially straightforward with no further modification processes. By and large, the sensor can provide a promising plan for the detection of l-histidine.

A highly sensitive luminescent probe based on Ru(II)-bipyridine complex for Cu2+, l-Histidine detection and cellular imaging

A ruthenium(II) bipyridyl complex conjugated with functionalized Schiff base (RuA) has been synthesized and functioned as a luminescent probe. The luminescence of RuA was greatly quenched by Cu2+ due to its molecular coordination with paramagnetic Cu2+. Subsequently, the addition of l-Histidine can turn on the luminescence of the RuA-Cu(II) ensemble, which can be attributed to the replacement of RuA in RuA-Cu(II) ensemble by l-Histidine. On the basis of the quenching and recovery of the luminescence of RuA, we proposed a rapid and highly sensitive on-off-on luminescent assay for sensing Cu2+ and l-Histidine in aqueous solution. Under the optimal conditions, Cu2+ and l-Histidine can be detected in the concentration range of 5 nM–9.0 μM and 50 nM–30 μM, respectively, and the corresponding detection limits were calculated to be 0.35 and 0.44 nM (S/N=3), separately. The proposed luminescent probe has been successfully utilized for the analysis of Cu2+ and l-Histidine in real samples (drinking water and biological fluids). Furthermore, the probe revealed good photostability, low cytotoxicity and excellent permeability, making it a suitable candidate for cell imaging and labeling in vitro.

Polydopamine Decorated Co3O4/Reduced Graphene Oxide Composite for Efficient and Selective Sensing of Histidine: Restructuring α-Cobalt Hydroxide to Highly Crystalline Co3O4 Sheets

Highly crystalline polydopamine decorated cobalt oxide (Co3O4) sheets, designated as CoPDA are synthesized from amorphous α-Co(OH)2 microspheres (CoMSPs) through acid restructuring process without pyrolysis or photo-irradiation. Structure and morphology of this product are analyzed by using spectroscopic and microscopic techniques. As-prepared CoPDA along with added reduced graphene oxide (r-GO) is explored for the electrochemical detection of L-histidine (L-His) under optimized condition. The electrochemical response of CoPDA/r-GO composite coated onto glassy carbon electrode toward the oxidation of L-His shows a linear response over a wide concentration range with a sensitivity of 4.47 μA/mM. The limit of detection value is calculated to be 1.5 μM with a response time of 17 seconds. The proposed sensor matrix is found to be highly selective for L-His among other interfering amino acids. Real sample analysis is carried out for the estimation of L-His using commercial L-His supplements. These studies suggest the promising use of this proposed electrochemical sensor for efficient detection of L-His possessing better selectivity and sensitivity.

Synthesis of imidazole-functionalized silicon quantum dots as “off-on” fluorescence probe for highly selective and sensitive detection of l-histidine

Silicon quantum dots have become attractive nanomaterials due to their excellent biocompatibility and optical performance. However, poor water-solubility greatly limits their wide applications. The paper reported a facile synthesis of imidazole-functionalized silicon quantum dots (termed as IF@SiQDs) via a simple hydrothermal method, in which N-trimethysilylimidazole and sodium citrate were used as the silicon precursor and reducing agent, respectively. The resulted IF@SiQDs exhibits good water-solubility, strong fluorescence with the quantum yield of 30.6% and high photo-stability. More importantly, the competitive coordination of Cu2+ between l-histidine (His) and IF@SiQD creates the unique fluorescence “off-on” response to His. Firstly, Cu2+ was firmly fixed at the surface of IF@SiQD by the coordination bond between Cu2+ and imidazole group to form stable Cum(IF@SiQD)n complex (m>1, n>1). A low concentration of His was introduced to partly replace the IF@SiQD in the complex to produce His-Cu-IF@SiQD, leading to an obvious fluorescence quenching. Based on the fluorescence “off” behaviour, IF@SiQD was developed as a novel optical nanosensor for the detection of His at a low level. Its peak fluorescence intensity linearly decreases with the increase the concentration of His in the range of 1.6×10−6–2×10−4M with the detection limit of 5.2×10−7M. Further, the His-Cu-IF@SiQD can be changed into non-fluorescent Cu(His)2 complex in the presence of a high concentration of His, accompanying the release of free IF@SiQDs and increase in the fluorescence intensity. Based on the fluorescence “on” behaviour, IF@SiQDs was used for the optical detection of His at a high level. Its peak fluorescence intensity linearly increases with the increase the concentration of His in the range of 2.8–5×10−3M with the detection limit of 2.2×10−3M. The as-proposed method provides the advantage of sensitivity, selectivity and linear range, it has been successfully applied in the fluorescent detection of His in human urine and in medical amino acid injection. The study also opens an avenue for the design of various functional silicon quantum dots that hold the great promise in the potential applications such as nanosensors, biocatalyst and cell imaging.

Ultra-Low Level Detection of L-Histidine Using Solution-Processed ZnO Nanorod on Flexible Substrate

This work demonstrates a novel label free and sensitive approach for the detection of L-histidine. This is a simple and reliable method for ultra-low level detection of L-histidine. All solution processed synthesizing technique was utilized to develop such type of detection scheme. Silicon substrate was replaced by normal transparent sheet to make it more facile and cost-effective detection technique. Fabricated device for L-histidine detection works upon the variation of current through the ZnO nanorod with L-histidine concentration. Operation principle strongly depends upon the electron charge transfer between metal cation and L-histidine inside the chelating complex. Morphological, structural and optical characterization of solution processed synthesized ZnO nanorod (ZnO NR) was carried out prior to sensor device fabrication. Our sensor device exhibits the sensitivity around 0.86 nA/fM and lower limit of detection (LOD) ∼ 0.1 fM(S/N=3).

A novel glutathione-stabilized silver–gold nano-alloy/Cu2+combination as a fluorescent switch probe forl-histidine

This paper reports the synthesis of glutathione-stabilized silver–gold nano-alloys and their use as a fluorescent switch probe for the detection ofl-histidine.

Inhibition of double-stranded DNA templated copper nanoparticles as label-free fluorescent sensors for l-histidine detection

A label-free fluorescent sensing strategy was reported for l-histidine detection by the inhibition of double-stranded DNA templated copper nanoparticles.

Cu2+ modulated silver nanoclusters as an on–off–on fluorescence probe for the selective detection of l-histidine

In the present study, a new strategy based on Cu2+ mediated DNA-templated silver nanoclusters (DNA-Ag NCs) was developed, as a label-free, on–off–on fluorescent probe for the detection of l-histidine. Eight synthesized DNA oligonucleotides (D1–D8) were experimentally tested, and D5-Ag NCs was finally selected for l-histidine detection due to its best fluorescent properties. The fluorescence emission of D5-Ag NCs could be quenched by Cu2+ via electron or energy transfer. Upon addition of l-histidine, the chelation between Cu2+ and the imidazole group of l-histidine leads to Cu2+ liberation from D5-Ag NCs, and subsequently results in a dramatic fluorescence enhancement of the probe. The method displayed a good selectivity toward l-histidine over all the other amino acids, with a linear relationship in the range of 0.20–80μM, and a limit of detection (LOD) of 4.3nM. The strategy was also successfully applied to detect l-histidine in diluted human urine, exhibiting great opportunities for practical application in biological system.

Cleaved DNAzyme substrate induced enzymatic cascade for the exponential amplified analysis of l-histidine

A novel strategy of cleaved DNAzyme substrate induced enzymatic cascade has been devised for the exponential amplified detection of l-histidine. The enzyme strand carries out hydrolytic cleavage of the substrate strand in the presence of l-histidine. The cleaved DNAzyme substrates introduce the polymerase/endonuclease reaction cycles as primers. The l-histidine acts as the activator for enzymatic cascade amplification generating a distinguishable fluorescence enhancement. A good nonlinear correlation (R=0.9994) between fluorescence intensity and the logarithm of the l-histidine concentration is obtained over the range from 50nM to 1.0mM. The detection limit was estimated as 30nM. This efficient amplification of the fluorescence signal is attributed to the l-histidine induced cooperation of Klenow Fragment polymerase (exo−) and Nb.BbvCI endonuclease reaction. The activation of such enzymatic cascades through analyte-DNAzyme interactions has a substantial impact on the development of exponential amplified DNAzyme sensors.