Detection Of Helicobacter Pylori Antibodies Research Articles

Frequency, distribution and determinants of Helicobacter pylori infection in adults and adolescents with gastric symptoms: cross-sectional epidemiological inquiry in district Haripur, Pakistan.

Helicobacter pylori (HP) is a vital element in the etiology of peptic ulcers and gastric cancer. This research aimed to determine the frequency, distribution, and determinants of HP infection in adults and adolescents with gastric symptoms in district Haripur, Khyber Pakhtunkhwa, Pakistan. This cross-sectional study was performed from June 2018 to June 2020 at the Medical Laboratory Technology Department, The University of Haripur, Pakistan. Presence of HP was a research variable, while sex, age groups, education status, overcrowding, dining habits, milk intake, drinking water source and animal contact were grouping variables. Immuno-chromatographic technique (ICT) was used to for serological detection of HP antibodies. All variables were represented by frequency and percentage with 95%CI. Prevalence of HP and its distribution by eight socio-demographic variables was testified by the chi-square goodness-of-fit test while association was testified by chi-square test of association. Out of total 1160 cases, 557 (48%) were positive for HP. Population prevalence was higher in men, in the age group 20-40 years, illiterate, family size ≤ 10 persons, taking restaurant food, using tetra pack, using municipal water, and having animal contact. The observed prevalence of HP was similar to its expected prevalence in the population. The observed distribution of HP in the sample was different from its expected distribution in population by eight socio-demographic variables. Presence of HP was associated with all eight socio-demographic variables besides age groups.

Detection of Helicobacter pylori antibodies in Patients with Chronic Liver Disease in Iraq

Detection of Helicobacter pylori antibodies in Patients with Chronic Liver Disease in Iraq

Helicobacter pylori infection in women with nonmenstrual migraine without aura: a case-control study

Background In the last two decades the relation between chronic Helicobacter pylori (HP) infection and migraine has been studied, but the results of these studies are controversial. Objective The aim of this study was to determine whether current or previous exposure to HP infection is a risk factor in women with nonmenstrual migraine (NMM) without aura, a concise subgroup in which environmental factors are dominant. Patients and methods A total of 24 women, with ages ranging from 19 to 60 years, affected by NMM without aura were evaluated in comparison with 24 control women, with ages ranging from 20 to 59 years, without any history of primary headache. In both cases and controls HP infection was evaluated by detection of HP antibodies in serum and HP antigen in stool. Results The prevalence of HP infection was nonsignificantly higher in the migraineurs without aura compared with controls ( P = 0.24 and 0.33). Regarding the intensity, duration, and frequency of attacks of migraine no differences were detected between the two groups. Conclusion Our findings do not support HP infection to be a risk factor for NMM without aura.

Accuracy of a Stick-type Kit and Enzyme-linked Immunosorbent Assay for the Detection of Helicobacter pylori Antibodies in Urine

Introduction: In Japan, both a novel stick-type kit and ELISA kit are available for detection of antibody to Helicobacter pylori in urine. Since urine samples can be obtained non-invasively and safely, these tests have been considered to be useful for the screening of H. pylori infection. However, only a small number of studies have estimated the accuracy of these tests. The aim of this study was to examine the clinical usefulness of this urine-based ELISA kit comparing with other non-invasive tests. Methods: Urine samples were obtained from 559 subjects who visited Sawada Clinic of Internal Medicine for the screening of gastric cancer. Subjects taking PPI and having previous gastric surgery were excluded. Urine samples from 359 subjects were tested using a novel stick-type urinary H. pylori antibodies detection kit (RAPIRUN®) while samples from 200 subjects were tested using an ELISA based test (URINELISA®). Prevalence of H. pylori infection was tested by 13C-urea breath test (UBT) and a monoclonal antibody-based stool antigen test (Testmate® Rapid Pylori Antigen: Rapid TPAg) using immunochromatography. Subjects were considered to be infected if at least one of UBT and rapid TPAg was tested positive. Results: The positivity of RAPIRUN and URINELISA was 41.8% (150 out of 359 subjects) and 37.0% (74 out of 200 subjects). Sensitivity and specificity of RAPIRUN were 83.3% and 67.0% while those of URINELISA were 87.8% and 85.7%, respectively. ELISA value of URINELISA was less than cut-off (1.0) in 117 subjects. There were 19 subjects whose ELISA value was between 0.4 to 0.99 and 4 of them (21.1%) were H. pylori infected. In 98 subjects ELISA value was less than 0.4, only 5 subjects were H. pylori infected (5.1%). Conclusion: The urine-based ELISA is accurate comparing with rapid stick-type kit and would be useful for the screening of H. pylori infection. If ELISA value was close to the cut-off, subjects should receive another test to detect H. pylori infection.

Design of polyglycidol-containing microspheres for biomedical applications

AbstractThe paper presents a short review on the synthesis, characterisation and selected medical applications of poly(styrene/α-tert-butoxy-ω-vinylbenzyl-polyglycidol) (P(S/PGL)) microspheres. The soap-free emulsion-polymerisation of styrene and α-tert-butoxy-ω-vinylbenzyl-polyglycidol macromonomer (PGL) in water yielded core-shell microspheres with a low particle-diameter dispersity (ratio of the weight average particle diameter and the number average particle diameter). The interfacial fraction of PGL units, estimated by XPS, was in the range of 0–42 mole % depending on the concentration of the macromonomer in the polymerisation feed. The studies of adsorption of model proteins showed that the surface fraction of adsorbed protein was significantly reduced when the PGL interfacial fraction was higher than 40 mole %. The P(S/PGL) particles with covalently immobilised proteins were used for the preparation of photonic crystal assemblies suitable for applications in optical biosensors and the medical diagnostic test for the detection of Helicobacter pylori antibodies in the blood serum.

Immunochromatographic detection of Helicobacter pylori antibodies in paediatric patients in Asaba, Nigeria.

Background: Helicobacter pylori has been implicated as one of the aetiological agents of gastrointestinal infections in children in developing countries. Paediatric patients attending Federal Medical Centre (FM«) and General Hospital (GH) Asaba, Nigeria, were serologically assessed for the infection.Methodology: 100 blood samples were aseptically collected by venepuncture from subjects and controls, and screened for Helicobacter pylori antibodies, using an immunochromatographic assay method.Results: An overall high prevalence rate of 55% was recorded. At the FMC, 8(20%) and 2(20%) prevalence was recorded for subjects and controls respectively, while at the GH, 14(35%) and 4(40%) were seropositive for the subjects and controls respectively.Conclusion: A causal relationship between gastrointestinal complaints and Helicobacter infection as well as asymptomatic carriage of infection amongst children in the study area has been serologically demonstrated. There is urgent need for routine screening of paediatric patients presenting with abdominal pains.

第23回日本総合健診医学会学術大会講演抄録集 総合健診における<I>Helicobacter Pylori</I>血清学的診断法の有用性

第23回日本総合健診医学会学術大会講演抄録集 総合健診における<I>Helicobacter Pylori</I>血清学的診断法の有用性

Evaluation of the performance of commercial test kits for detection of Helicobacter pylori antibodies in serum.

We have compared the sensitivities, specificities, and predictive values of three commercial serological assays for the diagnosis of Helicobacter pylori infection. A qualitative latex method (Pyloriset; Orion Diagnostics), a semiquantitative enzyme-linked immunosorbent assay (ELISA) (GAP test IgG; Bio-Rad), and a quantiative ELISA (Helico-G; Porton Cambridge) were used in 109 untreated dyspeptic patients. The presence of H. pylori was established when the results of culture and/or histology of the gastric biopsies taken were positive. The prevalence of H. pylori infection was 62% (52% in 42 patients younger than 45 years of age and 69% in 67 patients older than 45 years of age). Sensitivities and specificities were 68 and 76% for Pyloriset, 89 and 77% for GAP test IgG, and 82 and 83% for Helico-G. The positive predictive values for all three tests were between 85 and 90%. The predictive values for the absence of disease with a negative result were 62, 82, and 74% for Pyloriset, the GAP test, and Helico-G, respectively. With Helico-G in the younger group (less than 45 years), sensitivity significantly lower (71 versus 87%) and a positive predictive value lower than those for the older group (greater than 45 years) were found. Either the sensitivities and specificities of commercial methods for the measurement of antibodies to H. pylori in serum must be improved or the relationship between the presence of antibodies and the presence of bacteria in the stomach at the time of investigation is too weak to allow the use of serological techniques instead of culture and histological investigation of gastric biopsy material.