An active sensing approach using piezoceramic induced stress wave is proposed to provide monitoring and early warning for the development of interface debonding damage of precast segmental concrete beams (PSCBs). Three concrete specimens with toothed interfaces were fabricated and bonded with high-strength epoxy resin adhesive to form PSCBs. Smart aggregates (SAs) embedded in concrete specimens are used as actuators and sensors. The PSCBs are subjected to periodic loading with hydraulic jack to test the different degrees of debonding damage. The experimental results of time-domain and frequency-domain analysis clearly show that the amplitude of the signal received by the piezoceramic sensor is reduced when debonding crack occurs. The energy analysis and damage index based on wavelet packet can be used to determine the existence and severity of interface debonding damage in PSCBs. The experimental research validates the feasibility of monitoring the interface debonding damage in PSCBs using SA transducers based on active sensing technique.