Detection Of Dopamine Research Articles

Self-assembly of simple Schiff base ligand into unique saddle-type [4x4] tetranuclear architecture and its application as selective voltammetric dopamine sensor in aqueous conditions

Dopamine, a crucial catecholamine neurotransmitter, plays essential roles in the operation of the central nervous system in humans. Disrupted dopamine release is associated with neurological disorders and depression, therefore monitoring of dopamine levels is imperative for preliminary disease detection. Development of sensitive and selective sensors for neurotransmitters that function under aqueous conditions is however still challenging, mostly due to the complexity of hybrid nanomaterials that are interacting with the electrode. Here we provide a coordination compound constructed from simple substrates, where subcomponent self-assembly leads to a unique, discrete [4 × 4] saddle-type complex [Cu4(L-H)4(BF4)2(MeOH)2](BF4)2, which was characterized by ESI-MS and FT-IR techniques, including single crystal X-ray diffraction. The Cu4L4 complex was subsequently used for modification of the bare Au electrode based on its accumulation on the electrode surface. The new voltammetric sensor (Au/complex) was applied for dopamine detection alone and in the presence of interfering ascorbic acid by using the Differential Pulse Voltammetry (DPV) techniques under aqueous conditions. In the linear dynamic range (LDR) range from 0.0001 mM to 0.75 mM the dependence of the peak current on dopamine concentration satisfied the following linear regression equation: ip [mA] = 17∙10−2cDA [mM] + 8∙10−2 (R2 = 0.998). Moreover, the excellent limit of dopamine detection (LOD) and the limit of its quantification (LOQ) were established at the level of 5.4 nM and 18.0 nM with accomplished high sensitivity 0.17 A M−1, repeatability as well as reproducibility. Clear separation of the voltammetric signal of dopamine from this one of ascorbic acid, even in the presence of a 100-fold excess of interfering ions found in water, consequently proves that the new prepared sensor can be used as an excellent analytical tool for selective detection of dopamine and ascorbic acid coexisting in the tested samples.

A perylene diimide electrochemical probe with persulfate as a signal enhancer for dopamine sensing.

Dopamine (DA) is an important biomarker related to parkinsonism, schizophrenia and renal disease. Traditional electrochemical sensors for DA were based on the direct electrochemical oxidation of DA. In this paper, we report a new sensing strategy using N,N'-di(trimethylaminoethyl)perylene diimide (TMPDI) as an electrochemical probe and K2S2O8 as a signal enhancer for DA detection between 0 and -0.7 V with the DPV technique. MoS2 nanoflowers prepared by the hydrothermal method were used as a nanocarrier to load TMPDI. The reduction current of TMPDI was found to show a stepwise and significant increase at -0.24 V with the increase of concentration of K2S2O8 due to the continuous cycle of TMPDI molecules' electrochemical reduction and chemical oxidation. The presence of DA caused a large decrease of the reduction current of TMPDI due to the synergistic interaction of the competitive consumption of DA for K2S2O8 and the blocking effect of polyDA adhering to the electrode surface. The decreased current exhibited a linear response for DA from 10 pM to 100 μM with a detection limit of 4.1 pM and the proposed sensor showed high selectivity and excellent feasibility in human urine/serum sample detection.

Electrospun cobalt-doped 2D-MoSe2/polypyrrole hybrid-based carbon nanofibers as electrochemical sensing platforms

A novel cobalt-doped two-dimensional molybdenum diselenide/polypyrrole hybrid-based carbon nanofiber (Co/MoSe2/PPy@CNF) was prepared using the hydrothermal method followed by electrospinning technique. The structural and morphological properties of the 2D-TMD@CNF-based hybrids were characterized through X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), energy-dispersive spectrometry (EDS), and transmission electron microscopy (TEM). The Co-MoSe2/PPy@CNF exhibited large surface area, porous structure, and improved active sites due to the synergistic effect of the components. The electrochemical and electrocatalytic characteristics of the 2D-TMD@CNF-modified electrodes were also investigated using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The Co/MoSe2/PPy@CNF electrode was used as an electrochemical sensor for simultaneous detection of ascorbic acid (AA), dopamine (DA), and uric acid (UA) and showed enhanced catalytic activity and sensitivity. Using DPV measurements, the Co/MoSe2/PPy@CNF demonstrated wide linear ranges of 30–3212 μM for AA, 1.2–536 μM for DA, and 10–1071 μM for UA with low detection limits of 6.32, 0.45, and 0.81 μM, respectively. The developed sensor with the Co/MoSe2/PPy@CNF-modified electrode was also applied to a human urine sample and gave recoveries ranging from 94.0 to 105.5% (n = 3) for AA, DA, and UA. Furthermore, the Co/MoSe2/PPy@CNF-based sensor exhibited good selectivity and reproducibility for the detection of AA, DA, and UA.Graphical abstract

Fabrication of a graphite-paraffin carbon paste electrode and demonstration of its use in electrochemical detection strategies.

Electrochemical detection methods hold many advantages over their optical counterparts, such as operation in complex sample matrices, low-cost and high volume manufacture and possible equipment miniaturisation. Despite these advantages, the use of electrochemical detection is currently limited in the clinical setting. There is a wide range of potential electrode materials, selected for optimal signal-to-noise ratios and reproducibility when detecting target analytes. The use of carbon paste electrodes (CPEs) for electrochemical detection can be limited by their analytical performance, however they remain very attractive due to their low cost and biocompatibility. This paper presents the fabrication of an easy-to-make and use graphite powder/paraffin wax paste combined with a substrate produced via additive manufacturing and confirms its functionality for both direct and indirect electrochemical measurements. The produced CPEs enable the direct voltammetric detection of hexaammineruthenium(III) chloride and dopamine at an experimental limit of detection (ELoD) of 62.5 μM. The key inflammatory biomarker Interleukin-6 through an enzyme-linked immunosorbant assay (ELISA) was also quantified, yielding a clinically-relevant ELoD of 150 pg ml-1 in 10% human serum. The performance of low-cost and easy-to-use CPEs obtained in 0.5 hours is showcased in this study, demonstrating the platform's potential uses for point-of-need electroanalytical applications.

A silicon-doped carbon dot-based multivariate fluorometric and colorimetric probe for the simultaneous detection of heavy metal ions and dopamine

A schematic illustration of the synthesis of Si-CDs and the fluorometric and colorimetric multivariate sensing of Cu2+, Mn2+, Co2+, and DA.

Simultaneous and sensitive detection of dopamine and acetaminophen at a glassy carbon electrode modified with a carbonized-ZIF-67/Super P nanocomposite

Fabrication of C-ZIF-67/SP/GCE for the simultaneous detection of DA and AC.

A stable pyridone-based hydrogen-bonded organic framework for electrochemical detection of dopamine

A robust hydrogen-bonded organic framework was constructed using a 2-pyridone monomer by dual hydrogen bond interactions. The HOF-modified glass carbon electrode exhibited excellent electrochemical sensing performance for dopamine detection.

Tailoring cellulose paper via electroless CuSnB deposition for selective electrochemical detection of dopamine.

A novel, biodegradable substrate based, and cost-effective flexible electrochemical sensor was developed for the highly selective and sensitive detection of one of the major neurotransmitters, dopamine, which can be utilised as a disposable electrode for point-of-care diagnostic applications. The active material CuSnB decorated over cellulose paper exhibits good sensitivities of 3.92 μA μM-1 cm-2 with a limit of detection of 0.5 nM. Moreover, the flexible sensor demonstrated superior selectivity towards co-existing metabolites such as ascorbic acid, glucose, and uric acid, in addition to stability at various mechanical deformations.

Urchin-like CoP3/Cu3P heterostructured nanorods supported on a 3D porous copper foam for high-performance non-enzymatic electrochemical dopamine sensors.

In this study, we developed a high-performance non-enzymatic electrochemical sensor based on urchin-like CoP3/Cu3P heterostructured nanorods supported on a three-dimensional porous copper foam, namely, CoP3/Cu3P NRs/CF, for the detection of dopamine. Benefiting from the promising intrinsic catalytic activities of CoP3 and Cu3P, urchin-like microsphere structures, and a large electrochemically active surface area for exposing numerous accessible catalytic active sites, the proposed CoP3/Cu3P NRs/CF shows extraordinary electrochemical response towards the electrocatalytic oxidation of dopamine. As a result, the CoP3/Cu3P NRs/CF sensing electrode has a broad detection window (from 0.2 to 2000 μM), low detection limit (0.51 μM), high electrochemical sensitivity (0.0105 mA μM-1 cm-2), excellent selectivity towards dopamine in the coexistence of some interfering species, and good stability for dopamine determination. More importantly, the CoP3/Cu3P NRs/CF catalyst also exhibits excellent catalytic activity, sensitivity, and selectivity for dopamine detection under simulated human body conditions at a physiological pH of 7.25 (0.1 M PBS) at 36.6 °C.

Highly Deformable Double‐Sided Neural Probe with All‐in‐One Electrode System for Real‐Time In Vivo Detection of Dopamine for Parkinson's Disease (Adv. Funct. Mater. 4/2024)

Highly Deformable Double‐Sided Neural Probe with All‐in‐One Electrode System for Real‐Time In Vivo Detection of Dopamine for Parkinson's Disease (Adv. Funct. Mater. 4/2024)

Au nanoparticle decorated reduced graphene oxide and its electroanalytical characterization for label free dopamine detection

Au nanoparticle decorated RGO as effective nanocomposite for label-free electrochemical detection of dopamine.

SERS detection of dopamine using metal-chelated Ag nanoshell.

As the concentrations of different neurotransmitters can indicate the presence of certain disorders affecting brain functions, quantitative analyses of neurotransmitters have attracted increasing attention in various fields. Surface-enhanced Raman scattering (SERS) spectroscopy is an outstanding spectroscopic analytical tool that enables detection at the single molecule level with high specificity. As local field enhancement of surface plasmon is effective within nanometers, active interaction between SERS-active noble metals (gold and silver) and analyte molecules enhances the molecular detection capacity of SERS. However, neurotransmitters and noble metal nanoparticles are often not affinitive, because neurotransmitters generally have a hydroxyl group rather than a thiol group. As a result, the interaction between the two typically remains inactive, which makes detection more difficult. To overcome this limitation, in the present work we utilized metal-chelation to attract dopamine, a neurotransmitter molecule, close to the surface of silver nanoparticles. AgNS was capped with poly(vinyl alcohol) (PVA) and sequentially integrated with copper ion to bind dopamine in the form of chelate bonding between dopamine and copper. The PVA linked AgNS and metal ions through a coordinate bond between hydroxyl groups and metal ions. This metal-chelation-functionalized nanoprobe allowed us to stably detect dopamine in aqueous solution at a concentration of less than 10-6 M. Therefore, this method provides a convenient and easy-to-prepare option for the effective detection of dopamine, thus meaning it has the potential to be applied to other neurotransmitters.

Highly sensitive amperometric sensors based on laccase-mimetic nanozymes for the detection of dopamine.

The current research presents novel sensors based on laccase-like mimetics for the detection of dopamine (DA). The synthesized laccase-like nanozymes (nAuCu, nPtCu, nCuMnCo, and nCoCuCe) were prepared by a simple hydrothermal method and exhibited an attractive catalytic activity toward DA. The developed amperometric sensors based on laccase nanozymes (nAuCu and nPtCu) are more stable, selective, and revealed a higher sensitivity (6.5-fold than the biosensor based on the natural fungal laccase from Trametes zonata). The amperometric sensors were obtained by modification of the glassy carbon electrodes (GCEs) with AuPt nanoparticles. Functionalization of the electrode surface by AuPt NPs resulted in increased catalytic activity of the laccase-like layer and higher sensitivity. Among studied configurations, the sensor containing nAuCu and nAuPt possesses a wide linear range for dopamine detection (10-170 μM), the lowest limit of detection (20 nM), and the highest sensitivity (10 650 ± 8.3 A M-1 m-2) at a low applied potential (+0.2 V versus Ag/AgCl). The proposed simple and cost-effective sensor electrode was used for the determination of DA in pharmaceuticals.

Nanoengineered β-MnO2/rGO nanobead-based bioconjugate interfaces for the electrochemical detection of dopamine for the potential to manage neurological diseases and depression

In this study, we successfully developed a highly effective nanobead (nb) morphology-based β-MnO2/rGO nanocomposite via low-temperature hydrothermal synthesis.

Biopolymer-protected graphene-Fe3O4 nanocomposite based wearable microneedle sensor: toward real-time continuous monitoring of dopamine.

Neurological disorders can occur in the human body as a result of nano-level variations in the neurotransmitter levels. Patients affected by neuropsychiatric disorders, that are chronic require continuous monitoring of these neurotransmitter levels for effective disease management. The current work focus on developing a highly sensitive and personalized sensor for continuous monitoring of dopamine. Here we propose a wearable microneedle-based electrochemical sensor, to continuously monitor dopamine in interstitial fluid (ISF). A chitosan-protected hybrid nanomaterial Fe3O4-GO composite has been used as a chemical recognition element protected by Nafion antifouling coating layer. The morphological and physiochemical characterizations of the nanocomposite were carried out with XRD, XPS, FESEM, EDAX and FT-IR. The principle of the developed sensor relies on orthogonal detection of dopamine with square wave voltammetry and chronoamperometric techniques. The microneedle sensor array exhibited an attractive analytical performance toward detecting dopamine in phosphate buffer and artificial ISF. The limit of detection (LOD) of the developed sensor was observed to be low, 90 nM in square wave voltammetry and 0.6 μM in chronoamperometric analysis. The practical applicability of the microneedle sensor array has been demonstrated on a skin-mimicking phantom gel model. The microneedle sensor also exhibited good long-term storage stability, reproducibility, and sensitivity. All of these promising results suggest that the proposed microneedle sensor array could be reliable for the continuous monitoring of dopamine.

Preparation of carbon quantum dot fluorescent probe from waste fruit peel and its use for the detection of dopamine.

Carbon quantum dots (CQDs), as a new type of fluorescent nanomaterial, are widely used in the detection of small molecules. Abnormal dopamine secretion can lead to diseases such as Parkinson's disease and schizophrenia. Therefore, it is highly significant to detect dopamine levels in the human body. Using discarded fruit peels to prepare carbon quantum dots can achieve the reuse of kitchen waste, reduce pollution, and create value. Nitrogen-doped carbon quantum dots (N-CQDs) were prepared using the hydrothermal method, with orange peel as the raw material. The fluorescence quantum yield of N-CQDs reached a high value of 35.37% after optimizing the temperature, reaction time, and ethylenediamine dosage. N-CQDs were characterized using various techniques, including ultraviolet visible (UV-vis) spectroscopy, fluorescence spectrophotometer (PL), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FT-IR). These analyses confirmed the successful doping of nitrogen in the CQDs. The DA concentration ranged from 0 to 300 μmol L-1, and the linear equation for fluorescence quenching of N-CQDs was F/F0 = -0.0056c + 0.98647, with an R2 value of 0.99071. The detection limit was 0.168 μmol L-1. The recovery and precision of dopamine in rabbit serum were 98% to 103% and 2% to 6%, respectively. The prepared N-CQDs could be used as a fluorescent probe to effectively detect DA.

High-Fidelity Dopamine Detection With 2-D Material-Assisted and Plasmon-Enhanced Etched Core-Mismatched Optical Fiber Sensor

High-Fidelity Dopamine Detection With 2-D Material-Assisted and Plasmon-Enhanced Etched Core-Mismatched Optical Fiber Sensor

Water-stable perovskite CsPb2Br5/CdSe quantum dot-based photoelectrochemical sensors for the sensitive determination of dopamine.

A heterojunction of CdSe quantum dots in situ grown on the perovskite CsPb2Br5 (CsPb2Br5/CdSe) for water-stable photoelectrochemical (PEC) sensing was simply synthesized using the hot-injection method. Due to the inherent built-in electric field and the matching band structure between CsPb2Br5 and CdSe, the CsPb2Br5/CdSe p-n heterojunction demonstrates enhanced photoelectrochemical properties. Accelerated interfacial charge transfer and increased electron-hole pair separation enable hydrolysis-resistant CsPb2Br5/CdSe sensors to exhibit heightened sensitivity with an ultra-low detection limit (0.0124 μM) and a wide linear range (0.4-303.9 μM) in subsequent dopamine detection. Moreover, the CsPb2Br5/CdSe sensors show excellent anti-interference ability, as well as remarkable stability and reproducibility in water solvent. It is noteworthy that this work is conducted in an aqueous environment, which provides an inspiring and convenient way for photoelectric and photoelectrocatalysis applications based on water-resistant perovskites.

A novel Ga2O3-doped ZnO decorated SWCNT nanocomposite based amperometric sensor for efficient detection of dopamine in real samples

Our current research focuses on developing an efficient electrochemical Dopamine sensor for diagnosing neurological disorders related to abnormal Dopamine levels. We utilized a Ga2O3-doped ZnO decorated SWCNT (Ga2O3·ZnO@SWCNT) nanocomposite to modify a glassy carbon electrode. The structure and morphology of the Ga2O3·ZnO@SWCNT nanocomposite was examined utilizing a variety of approaches to ensure of their appropriateness as the sensing nanomaterial. The sensor effectively detects Dopamine within a wide range (1.0–2056 μM) in neutral phosphate buffer solution, exhibiting high sensitivity (0.2536 μAμM−1cm−2) and a practical detection limit (0.052 μM). It accurately quantifies Dopamine in human blood serum and commercial dopamine injection samples, displaying excellent reproducibility, repeatability, and stability. This proposed Dopamine sensor proves to be a reliable tool for Dopamine determination.

A dual-functional cuprum coordination framework for high proton conduction and electrochemical dopamine detection.

The present study selected 5, 5'-((6-(ethylamino)-1, 3, 5-triazine-2, 4-diyl) bis(azanediyl))diisophthalic acid (H4EATDIA) as ligand and an amino-functionalized cuprum-based MOF (EA-JUC-1000), successfully synthesized by microwave-assisted method, for proton conduction and dopamine sensing applications. In order to enhance the proton-conducting potential of EA-JUC-1000, the Brönsted acid (BA) encapsulated composites (BA@EA-JUC-1000) are dopped into chitosan (CS) to form a series of hybrid membranes (BA@EA-JUC-1000/CS). The impedance results display that the best proton conductivity of CF3SO3H@EA-JUC-1000/CS-8% reaches up to 1.23 × 10-3 S∙cm-1 at 338K and ~ 98% RH, 2.6-fold than that of CS. Moreover, the EA-JUC-1000 is in-situ combined with reduced graphene oxide (rGO) (rGO/EA-JUC-1000), which makes EA-JUC-1000 have a wide detection range (0.1 ~ 500μM) and a low limit of detection (50nM), together with good anti-interference performance, reproducibility and repeatability. In addition, the electrochemical sensing method has been successfully applied to detect DA in bovine serum samples. The dual-functional MOF-based hybrid membrane and composites including proton conduction and DA sensing would provide an example of practical application for MOFs.