This paper describes experimental research aimed at improving our understanding of the complex unsteady three-dimensional flow field associated with the interaction between a pump impeller and its vaned diffuser. The paper provides the results of experiments carried out using Laser Particle Tracking Velocimetry (LPTV) and Laser Doppler Anemometry (LDA), in which time-resolved details of the unsteady flow field in a vaned diffuser of a medium specific speed pump have been obtained as a function of the local position of the pump impeller blades. Detailed flow field measurements have been carried out at several measurement positions in the diffuser and at a number of operating points along the pump characteristic. The measurement results have been analyzed to elucidate some interesting flow features observed in this typical pump diffuser. These include three-dimensional flow at the impeller outlet, flow separation in the diffuser channel, unsteady recirculation of the flow from the diffuser into the impeller, the passage of vorticity in the impeller blade wakes through the diffuser, and periodic unsteadiness and turbulence in the diffuser flow channel. The relevance of these flow features to the stability of the pump characteristic is discussed.