A precision instrument building is used to house various experimental equipments that are sensitive to ground vibrations. In order to research the characteristics of ground vibrations under the moving vehicle load, a field test of vibration was carried out. A coupling model of moving vehicle load-foundation soil-building structure was established to simulate vibration isolation measures and analyze the vibration isolation effect. The results indicate that the ground vibrations in the sensitive area caused by the moving vehicle load are mainly low frequency in the vertical direction, where the principal frequency is in the 3∼4 Hz range, consistent with the ground pulsation test. The horizontal vibration attenuates much faster than the vertical vibration. For the vibration isolation using the piles, factors including pile spacing, pile length and barrier width have a significant impact on the vibration isolation effect, while the number of pile rows and the material of piles have relatively little influence. Using the optimized vibration isolation plan, the vibration in the sensitive area caused by the moving vehicle load can be reduced by about 30∼50%. The research methods and conclusions of this paper can serve as a useful reference for the analysis of ground vibrations and vibration isolation design in vibration-sensitive areas.