The irregularity excitation experienced by subway vehicles is mainly the result of the interaction between the track and wheel. However, in the early system design and simulation analysis of subway vehicles, most only used the traditional standard track irregularity spectrum as the input excitation, ignoring or underestimating the contribution of the wheel irregularity. Based on our statistical analysis of 200,000 kilometers of tracking test data of subway vehicle wheel irregularities, we found that the short-wave irregularity caused by the wheels far exceeds the traditional standard track irregularity. The service condition of the vehicle is seriously affected, especially in the final stage of a wheel re-profile period. To address the above issues: Firstly, the sensitive wavelength range (16. 67∼2500 mm) of subway vehicles was derived based on the axle box acceleration spectrum of IEC61373: 2010, which was very close to the wavelength range (50∼2627 mm) of the wheel irregularity spectrum proposed later, demonstrating the importance of compiling a wheel irregularity spectrum; Secondly, based on the large number of tracking test data of wheel out-of-roundness, a calculation method of the wheel irregularity quantile spectrum under the Johnson non-normal transformation system was proposed; Thirdly, according to the different stages of the wheel re-profile period, the wheel irregularity spectrum is introduced to correct the short-wave segments of the traditional standard track irregularity spectrum to compile a wheel-rail comprehensive irregularity spectrum.