Karst rocky desertification is a major ecological disaster faced by the southeastern region of Yunnan Province, in China. The research of land use status in rocky desertification area is more and more concerned by human. Through remote sensing integrated survey ways to establish the spectral database of six kinds of typical land types, such as water, forest land, paddy field, grassland, dry land and bare land; then, Normalized Difference Vegetation Index (NDVI) processing and Tasseled Cap transformation processing for the spectral data; finally, the spectral characteristics and the variation of typical land types was analyzed and studied. The results indicated that, there are no significant differences of spectral of the six kinds of typical land types, after the processing of NDVI, the water, the forest land and grassland with larger vegetation coverage, and the paddy land, dry land and bare land with smaller vegetation coverage, can be well distinguish, but the distinction between forest land and grassland is bad, the distinction of paddy land, dry land and bare land is also bad; after the Tasseled Cap transformation processing, the dispersion degree of water, forest land, paddy field and grassland is better, the dispersion degree of dry land and bare land is a bit poor, need to be further studied. The remote sensing integrated survey and the analysis of spectral properties are important foundation for the Land Use / Land Cover Change (LUCC) and the dynamic monitoring of rocky desertification in southeastern rocky desertification region of Yunnan province.