Desert Soil Research Articles

Inside the Atacama Desert: uncovering the living microbiome of an extreme environment

ABSTRACT The Atacama Desert in Chile is one of the driest and most inhospitable places on Earth. To analyze the diversity and distribution of microbial communities in such an environment, one of the most important and challenging steps is DNA extraction. Using commercial environmental DNA extraction protocols, a mixture of living, dormant, and dead cells of microorganisms is extracted, but separation of the different DNA pools is almost impossible. To overcome this problem, we applied a novel method on soils across a west–east moisture transect in the Atacama Desert to distinguish between extracellular DNA (eDNA) and intracellular DNA (iDNA) at the cell extraction level. Here, we show that a large number of living and potentially active microorganisms, such as Acidimicrobiia , Geodermatophilaceae , Frankiales , and Burkholderiaceae, occur in the hyperarid areas. We observed viable microorganisms involved as pioneers in initial soil formation processes, such as carbon and nitrogen fixation, as well as mineral-weathering processes. In response to various environmental stressors, microbes coexist as generalists or specialists in the desert soil environment. Our results show that specialists compete in a limited range of niches, while generalists tolerate a wider range of environmental conditions. Use of the DNA separation approach can provide new insights into different roles within viable microbial communities, especially in low-biomass environments where RNA-based analyses often fail. IMPORTANCE The novel e- and iDNA separation technique offers insights into the living community at the cell extraction level in the hyperarid Atacama Desert. This approach provides a new framework for analyzing the composition and structure of the potentially active part of the microbial communities as well as their specialization, ecological network and community assembly process. Our findings underscore the significance of utilizing alternative genomic techniques in low-biomass environments where traditional DNA- and RNA-based analyses may not be feasible. The results demonstrate the viability of the proposed study framework and show that specialized microorganisms are important in initial soil formation processes, including microbial-driven mineral weathering, as well as the fixation of carbon and nitrogen.

Divergent mechanisms driving nutrient stoichiometry in surface and deep soils of desert ecosystems on the Qinghai–Tibetan plateau

The ecological stoichiometric properties of desert soils determine nutrient availability and contribute to biodiversity and ecosystem stability. We obtained 1784 soil samples from 330 soil profiles distributed across the desert area of the Qinghai–Tibet Plateau (QTP). We measured the content of soil organic carbon (SOC), total nitrogen (TN), total phosphorus (TP), total potassium (TK), alkali-hydrolyzed nitrogen (AN), available phosphorus (AP), and available potassium (AK) to elucidate the vertical patterns of soil stoichiometric characteristics and their driving factors. We found a unique vertical distribution of SOC and soil nutrients in the QTP desert ecosystem: the proportions of SOC and nutrient storage in the topsoil (0–20 cm) were the highest that have been reported to date, especially for the proportion of SOC, which reached 54 %. The vertical distributions of SOC and AN fit an exponential function (r = 0.247 and 0.249), TN fit a linear function (r = 0.115), and TP, AP, and AK fit a curvilinear function (r = 0.127, 0.175, and 0.103), indicating that soil components exhibited a depth-dependent distribution in the alpine desert ecosystem. With increasing soil depth, C:N and C:P decreased significantly, AN:AP first increased and then decreased, and AP:AK first decreased and then increased. The factors that influenced soil nutrients and stoichiometry differed between the topsoil (0–20 cm) and deep soil (20–100 cm) layers. In the topsoil (0–20 cm), vegetation and precipitation were the dominant factors for soil nutrients and stoichiometry, while topography and climate affected soil nutrients and stoichiometry indirectly by influencing vegetation growth. In the deep soil (20–100 cm), silt particles and precipitation were the dominant factors for soil nutrients and stoichiometry, but climate affected soil nutrients and stoichiometry indirectly, mainly by influencing soil texture. The inconsistencies of nutrient stoichiometric driving mechanisms in surface and deep soils contributed to a comprehensive understanding of soil stoichiometric properties at the profile scale of desert ecosystems.

In-vitro antibacterial and antibiofilm activities and in-silico analysis of a potent cyclic peptide from a novel Streptomyces sp. strain RG-5 against antibiotic-resistant and biofilm-forming pathogenic bacteria.

The proliferation of multidrug-resistant and biofilm-forming pathogenic bacteria poses a serious threat to public health. The limited effectiveness of current antibiotics motivates the search for new antibacterial compounds. In this study, a novel strain, RG-5, was isolated from desert soil. This strain exhibited potent antibacterial and antibiofilm properties against multidrug-resistant and biofilm-forming pathogenic bacteria. Through phenotypical characterizations, 16S rRNA gene sequence and phylogenetic analysis, the strain was identified as Streptomyces pratensis with 99.8% similarity. The active compound, RG5-1, was extracted, purified by reverse phase silica column HPLC, identified by ESI-MS spectrometry, and confirmed by 1H and 13C NMR analysis as 2,5-Piperazinedione, 3,6-bis(2-methylpropyl), belonging to cyclic peptides. This compound showed interesting minimum inhibitory concentrations (MICs) of 04 to 15µg/mL and minimum biofilm inhibitory concentrations (MBICs 50%) of ½ MIC against the tested bacteria. Its molecular mechanism of action was elucidated through a molecular docking study against five drug-protein targets. The results demonstrated that the compound RG5-1 has a strong affinity and interaction patterns with glucosamine-6-phosphate synthase at - 6.0kcal/mol compared to reference inhibitor (- 5.4kcal/mol), medium with penicillin-binding protein 1a (- 6.1kcal/mol), and LasR regulator protein of quorum sensing (- 5.4kcal/mol), confirming its antibacterial and antibiofilm activities. The compound exhibited minimal toxicity and favorable physicochemical and pharmacological properties. This is the first report that describes its production from Streptomyces, its activities against biofilm-forming and multidrug-resistant bacteria, and its mechanism of action. These findings indicate that 2,5-piperazinedione, 3,6-bis(2-methylpropyl) has the potential to be a promising lead compound in the treatment of antibiotic-resistant and biofilm-forming pathogens.

SPATIAL VARIABILITY OF THE HEAT EMITTED FROM THE LAND SURFACE IN DETERMINING THE CHARACTERISTICS OF DESERT SOILS IN AL-SAMAWAH DESERT USING “GIS”

This study was aimed to reveal the variation land surface temperature "LST" with some characteristics of desert soils in the Samawa desert at Al-Muthanna province, and to adopt solutions and treatments by identifying factors and indicators that have a direct impact to desertification, by using geographic information systems mapping for spatial distribution Some physical and chemical properties were selected. . the result showed that there was a statistical relationship between LST and the physical characteristics, as it was revealed that the comparison of sand and silt content with the land surface temperature "LST" . There is a significant relationship with the determination coefficient of R2=0.6. The chemical properties, the results showed that there was a significant connection between the electrical conductivity with the land surface temperature with a coefficient of determination R2=0.5 and an It was also observed that there is a significant relationship between the land surface temperature and the temperature measured in the field with a determination coefficient R2=0.6.

Streptomyces sp. from desert soil as a biofactory for antioxidants with radical scavenging and iron chelating potential

Iron homeostasis is vital for normal physiology, but in the majority of circumstances, like iron overload, this equilibrium is upset leading to free iron in the plasma. This condition with excess iron is known as hemochromatosis, which has been linked to many side effects, including cancer and liver cirrhosis. The current research aimed to investigate active molecules from Streptomyces sp. isolated from the extreme environment of Bahawalpur deserts. The strain was characterized using 16 S rRNA sequencing. Chemical analysis of the ethyl acetate cure extract revealed the presence of phenols, flavonoids, alkaloids, and tannins. Multiple ultraviolet (UV) active metabolites that were essential for the stated pharmacological activities were also demonstrated by thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Additionally, Gas chromatography/mass spectrometry (GC-MS) analysis revealed the primary constituents of the extract to compose of phenol and ester compounds. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to assess the extract’s antioxidant capacity, and the results showed a good half-maximal inhibitory concentration (IC50) value of 0.034 µg/mL in comparison to the positive control ascorbic acid’s 0.12 µg/mL. In addition, iron chelation activity of extract showed significant chelation potential at 250 and 125 µg/mL, while 62.5 µg/mL showed only mild chelation of the ferrous ion using ethylene diamine tetra acetic acid (EDTA) as a positive control. Likewise, the extract’s cytotoxicity was analyzed through 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using varying concentrations of the extract and showed 51% cytotoxicity at 350 µg/mL and 65% inhibition of cell growth at 700 µg/mL, respectively. The bioactive compounds from Streptomyces sp. demonstrated strong antioxidant and iron chelating potentials and can prolong the cell survival in extreme environment.

Sewage sludge as soil amendment in arid soils - A trace metal, nutrient and trace organics perspective

Sewage sludge management has emerged as a critical environmental challenge due to the large volumes generated globally. Valorization techniques, including energy production and agricultural applications, offer sustainable solutions, particularly in regions with low soil fertility. The sewage sludge utilization in the Middle East region is low. This paper presents a pragmatic risk-based assessment using the risk-based corrective action approach to evaluate sludge application in desert soils. This methodology focuses on the source-pathway-receptor interaction and assesses the likelihood of contaminants posing a real threat. In arid desert regions like Kuwait, where soil organic content and moisture are extremely low, the application of sewage sludge presents a feasible option to enhance soil quality and valorize unutilized sludge dumps which pose significant environmental concerns but are left to desiccate in the absence of any environmental regulation towards its utilization and due to religious apprehensions. Since the sludge characterization is not well detailed a brief review of the available data was included to establish the bounds of various organic, metal and nutrients that were used for generating the model. This study examines the changes in the physico-chemical properties of desert soils following sludge application, focusing on the likely fate of trace metals and organic contaminants. The alkaline desert soils of Kuwait, with a pH range of 7.7–8.9, are particularly suitable for sludge application due to the low mobility of metals in alkaline conditions. Additionally, sludge application lowers soil pH, improving conditions for plant growth. The region's deeper water table and scant annual precipitation (<0.15 m) further reduce the risk of groundwater contamination and deeper soil profile contamination. The presence of organic content, nitrates, Zn, and Cu in sludge can promote native vegetation growth. However, trace organic contaminants, including PAHs, PCBs, and pharmaceuticals, pose a potential risk to soil contamination, but since the geological section shows intervening impervious layers the contamination is going to be localized, even if there is sufficient leachable fraction. Given the minimal risk of contamination under the unique conditions of arid regions, this approach highlights the potential for eco-friendly sludge valorization, that will improve vegetation cover and arrest the suspended particulate suspension. However, before the large-scale implementation of this modelled concept, a detailed experimental study on the pilot scale or lysimeters is recommended to assess the long-term impacts of sludge application and to obtain data that can inform policy guidelines for sustainable sludge management in desert environments.

Optimization and performance analysis of a novel automatic planting-irrigating integrated robot

Soil desertification is a severe environmental problem that seriously threatens the ecological environment and sustainable development. Traditional tree-planting methods face challenges such as insufficient labor force and low efficiency. Based on these problems, this study designed a novel automatic planting-irrigation integrated robot with automatic planting, irrigating, and monitoring functions. The robot obtains land information through laser triangle ranging technology, the camera, and other sensors. Machine vision and deep learning algorithms are applied for image processing and identification to identify the areas suitable for tree planting accurately. The drip irrigation module adopts the DHT11 digital temperature and humidity sensor control switch, which can realize efficient tree planting and watering operation, and ensure the growth of saplings and has a broad application prospect. Our design is meaningful in restraining soil desertification, maintaining the ecological environment, and realizing a sustainable economy.

The inclusion of clay minerals accelerates biocrust formation and potentially boosts carbon storage capabilities

Biocrusts, prevalent in desert areas, play a pivotal role in mitigating soil degradation, shaping a common natural landscape. However, amidst climate change and human activities, biocrust coverage is encountering the risk of degradation. The restoration of degraded biocrusts and the construction of artificial ones are regarded as promising technologies for combating soil degradation. Although clay minerals are a crucial abiotic factor in biocrust formation and are directly influence their development and succession, they have not gained widespread attention. The aim of this study was to investigate the mechanism of action of clay minerals in biocrusts. Various quantities of sepiolite were amalgamated with cyanobacteria—Microcoleus vaginatus (ratios of 1:0, 1:10, 1:20, 1:50, 1:100; cyanobacteria dry weight to sepiolite weight), to construct artificial biocrusts and observe the formation and development of them. The results showed that the addition of small quantities of sepiolite (ratios of 1:10 and 1:20) not only facilitated the accumulation of cyanobacterial biomass (1.47–1.86 times) and exopolysacchrides (EPS) (1.73–2.58 times) compared to the control (ratio of 1:0), but also notably enhanced the accumulation of total carbon, total organic carbon, and microbial biomass carbon, highlighting its potential in enhancing the carbon sequestration capabilities of biocrusts. Scanning electron microscope (SEM) analysis revealed that sepiolite serves as a “receiver and bridge” within the biocrust structure, enhancing its compactness and stability, thereby fostering the growth of cyanobacteria and facilitating nutrient transport. Further, Fourier Transform Infrared (FTIR) and X-Ray Photoelectron Spectroscopy (XPS) analysis reviewed changes in some functional groups of EPS and sepiolite after mixing, validating that EPS can function as a “binder” to unify particles. Our findings demonstrate that the incorporation of clay minerals can facilitate biocrust formation, presenting a practical and economical approach, and providing a novel perspective for constructing artificial biocrusts and rehabilitating degraded desert soils.

Inorganic Carbon Pools and Their Drivers in Grassland and Desert Soils.

Inorganic carbon is an important component of soil carbon stocks, exerting a profound influence on climate change and ecosystem functioning. Drylands account for approximately 80% of the global soil inorganic carbon (SIC) pool within the top 200 cm. Despite its paramount importance, the components of SIC and their contributions to CO2 fluxes have been largely overlooked, resulting in notable gaps in understanding its distribution, composition, and responses to environmental factors across ecosystems, especially in deserts and temperate grasslands. Utilizing a dataset of 6011 samples from 173 sites across 224 million hectares, the data revealed that deserts and grasslands in northwestern China contain 20 ± 2.5 and 5 ± 1.3 petagrams of SIC in the top 100 cm, representing 5.5 and 0.76 times the corresponding soil organic carbon stock, respectively. Pedogenic carbonates (PIC), formed by the dissolution and re-precipitation of carbonates, dominated in grasslands, accounting for 60% of SIC with an area-weighted density of 3.4 ± 0.4 kg C m-2 at 0-100 cm depth, while lithogenic carbonates (LIC), inherited from soil parent materials, prevailed in deserts, constituting 55% of SIC with an area-weighted density of 7.1 ± 1.0 kg C m-2. Soil parent materials and elevation determined the SIC stocks by regulating the formation and loss of LIC in deserts, whereas natural acidification, mainly induced by rhizosphere processes including cation uptake and H+ release as well as precipitation, reduced SIC (mainly by PIC) in grasslands. Overall, the massive SIC pool underscores its irreplaceable role in maintaining the total carbon pool in drylands. This study sheds light on LIC and PIC and highlights the critical impact of natural acidification on SIC loss in grasslands.

Effects of Agricultural Land Use on Desert Soil Classification in Holy Karbala Governorate

This study was conducted to evaluate physical, chemical, and morphological features to classify soils at the subgroup level. The research area, located west of Karbala, consists of three locations, each comprising pedons in both cultivated and uncultivated lands. GPS coordinates were recorded, and morphological descriptions of pedon horizons were made in accordance with the American Soil Survey Manual. This study investigates the effect of agricultural land use on the classification of desert soils in the Holy Karbala Governorate. The results revealed that the dry climate caused diversity in the morphological features of the soils, mainly in horizon type, thickness, and arrangement. Agricultural usage had an impact on diagnostic horizons, particularly the gypsic horizon, and features such as soil color, structure, organic matter, carbonate minerals, gypsum, and root distribution. However, there was no substantial impact on soil texture development, and soils did not show a consistent pattern of soil separation distribution with depth, indicating weak pedogenic processes. The most common texture classes were loamy sand, sandy loam, and sand, with sand contents ranged from 902.08 to 574.12 g kg. Soil bulk density differed from 1.67 to 1.30 Mg m³, with lower values in farmed areas. Chemical properties were influenced, with soil pH ranging from 8.36 to 7.54, electrical conductivity (EC) from 5.46 to 2.03 dS m, cation exchange capacity (CEC) from 15.25 to 2.05 meq.100g, organic matter from 43.51 to 1.18 g kg, carbonate minerals from 461.18 to 174.06 g kg, and calcium sulfate from 182 to 21 g kg. The soils were classed as Aridisols because they were found in a dry desert with an Aridic moisture regime. The presence of distinctive saline layers such as Calcic and Gypsic was discovered, resulting in the classification of cultivated soils as Typic Haplocalcids and uncultivated lands as Typic Calcigypsids.

Recovery of bacterial network complexity and stability after simulated extreme rainfall is mediated by K−/r-strategy dominance

The complexity and stability of belowground microbial communities are among the core mechanisms that drive ecosystem functions. Disturbances, such as climate extremes, can exert a substantial influence on these microbial attributes. However, the role of the microbial life history strategy in determining the complexity and stability of desert soil microbial communities after extreme rainfall is poorly understood. Here, we explored the response patterns of bacterial network complexity and stability and characterized the bacterial life history strategy on the basis of the oligotroph (K-strategist)-to-copiotroph (r-strategist) ratio after three extreme rainfall treatments (11, 15, and 25 mm). Experimental results demonstrated that the bacterial communities immediately shifted from the K-strategy to the r-strategy one day after extreme rainfall and gradually recovered to the K-strategy over time, as verified by the increase (31.46 %–125.51 %) then decrease (41.51 % – 95.78 %) in the weighted rrn copy number. Meanwhile, bacterial network complexity and stability showed three-phase patterns [dramatic decline (decreased by 211.67 %–388.46 %)–gradual recovery (increased by 45.60 %–103.65 %)–insignificant change] after three extreme rainfall events. The decline/recovery of these bacterial attributes was highly pronounced/slow after the large extreme rainfall treatment. Specifically, the responses of bacterial network complexity and stability to extreme rainfall showed negative correlations with the response of the weighted rrn copy number (R = -0.55 – -0.74, P < 0.01). The mechanisms underlying the response patterns of bacterial network complexity and stability were related to the prevalence of the r-strategy of the bacterial communities (Phase 1) and the shifts from the r-strategy to the K-strategy due to the gradual reduction in soil moisture (Phase 2 and 3). These results emphasize the importance of the microbial life history strategy in maintaining network complexity and stability and help in understanding how belowground communities can withstand future disturbances.

Dynamics of soil water, temperature, and salt and their coupled effects in Haloxylon ammodendron forests of different ages in an arid desert oasis ecotone

Study regionThe study region is a typical desert oasis ecotone of the Hexi Corridor, Northwest China. Study focusBased on four years of in situ observational data of soil water, temperature, and electrical conductivity obtained from three Haloxylon ammodendron (H. ammodendron) forests (20, 30 and 50 years) in a typical desert oasis ecotone (DOE) of the Hexi Corridor, in this study, the mechanisms of change and coupled effects of water, heat, and salt in frozen desert soils along a long-term shrub plantation in a typical desert oasis ecotone are presented. New hydrological insights for the regionThis study revealed that changes in soil water, heat, and salt and their coupling effects were completely different between shallow (0–80 cm) and deep (160–200 cm) depths. At 0–80 cm, soil water gradually decreased in the 50-year-old H. ammodendron plot, but soil salt first increased in the 30-year plot and then decreased in the 50-year plot. When the freezing process occurred in the 0–80 cm depth interval, the soil water and salt contents decreased nonlinearly with the absolute value of the soil temperature (|T|), following a power function and logarithmic function, respectively. For the thawing process in the 0–80 cm depth interval, soil water and salt increased with increasing temperature, and there was a significant linear relationship between soil water and salt (P<0.01). In the 160–200 cm layer, soil water and salt significantly increased after 30–50 years during the growing season.

Physical analysis and inverse methods applied to archaeological FIRE replications on ATACAMA desert soils, northern Chile

Physical analysis and inverse methods applied to archaeological FIRE replications on ATACAMA desert soils, northern Chile

A nationwide study of heavy metal(loid)s in agricultural soils and the soil-grown black morel Morchella sextelata in China

The accumulation of heavy metal(loid)s (HMs) in soil-grown mushrooms poses potential health risks. Morchella sextelata (black morel) is a typical soil-grown mushroom with a rapidly expanding cultivation area. This study investigated the distribution of arsenic, cadmium, chromium, copper, mercury, nickel, lead, and zinc in 213 pairs of soil and morel samples collected from 29 provincial administrative regions in China, together with the nutritional contents in the morel samples. The HM contents in the arable soils used to cultivate morels were 2.4–33.1 times higher than those in desert soils, while the HM contents in arable-soil morels were 2.9–155.9 times higher than desert morels. The HM contents of morels and their cultivation soils were significantly correlated (0.465 ≤ R ≤ 0.778, P < 0.001). Furthermore, the enrichment factors of most HMs were higher in arable soils than in desert soils (P < 0.05), except Hg. A considerable proportion of the arable soils produced morels with HMs exceeding the risk control standards (RCSs) for food and the health-risk thresholds of dietary intake. In comparison, HMs in morels from desert soils were far below the RCSs and health-risk thresholds. In addition, desert morels contained higher contents of crude proteins, total polysaccharides, and free amino acids (P < 0.001). These findings indicate that growing morels in desert soils is a way of green production that provides mushroom products with improved safety and nutrition.

Functional characterization of maize phytochrome-interacting factor 3 (ZmPIF3) in enhancing salt tolerance in arabidopsis

Soil salinization, a prevalent form of environmental stress, leads to significant soil desertification and impacts agricultural productivity by altering the internal soil environment, slowing cellular metabolism, and modifying cellular architecture. This results in a marked reduction in both the yield and diversity of crops. Maize, which is particularly susceptible to salt stress, serves as a critical model for studying these effects, making the elucidation of its molecular responses essential for crop improvement strategies. This study focuses on the phytochrome-interacting factor 3 (PIF3), previously known for its role in freezing tolerance, to assess its function in salt stress tolerance. Utilizing two transcript variants of maize ZmPIF3 (ZmPIF3.1 and ZmPIF3.2), we engineered Arabidopsis transgenic lines to overexpress these variants and analyzed their phenotypic, physiological, biochemical, and transcriptomic responses to salt stress. Our findings reveal that these transgenic lines displayed not only enhanced salt tolerance but also improved peroxide decomposition and reduced cellular membrane damage. Transcriptome analysis indicated significant roles of hormonal and Ca2+ signaling pathways, along with key transcription factors, in mediating the enhanced salt stress response. This research underscores a novel role for ZmPIF3 in plant salt stress tolerance, offering potential avenues for breeding salt-resistant crop varieties.

Efficiency of Fulvic Acid for Improving Physico-Chemical Properties of Albic Black, Gansu Desert and Shahjiang Black Soils

Fulvic acids are a crucial component of soil that influence various chemical, biological, and physical aspects of soils as well as increase nutrient availability. We examined that application of plant-derived liquid (PDL) fulvic acid on three low obstacle, typical Chinese soil can improve the soil fertility and enhance the plant growth and nutrients uptake. The pot experiments were carried out on three different Albic (AL), Irrigated Desert (IR) and Shahjiang Black (SH) soils, the Plant-derived Liquid (PDL) fulvic acid (FA) was applied at rates of 0.50% as well as control applied at 0% along with three replications. The results showed that PDL FA significantly increases the SOC content in three soils highest SOC was detected at SH, however the SOC fractions were significantly decreased in AL, SH and no-significant difference was recorded at IR. Similarly, the soil pH was significantly increased in AL and decreased at IR and SH, however, the electrical conductivity of IR decreased and increased in AL and SH comparing with initial soil pH and electrical conductivity. The results also examine that available nitrogen, phosphorus of AL, IR and SH soils were increased, however, available potassium content was decreased in IR and increased at AL and SH comparing with initial values. The highest Ca and Mg content was observed in SH and organic degree compound were found 26.7% AL, 49.2% IR and 18.2%, SH soil, conversely the organic-inorganic composite was observed lower. This study suggests that the PDL FA significantly increases the nutrient content of AL, IR and SH soils comparing with initial soil properties.

Metagenomic insights into the prokaryotic communities of heavy metal-contaminated hypersaline soils

Saline soils and their microbial communities have recently been studied in response to ongoing desertification of agricultural soils caused by anthropogenic impacts and climate change. Here we describe the prokaryotic microbiota of hypersaline soils in the Odiel Saltmarshes Natural Area of Southwest Spain. This region has been strongly affected by mining and industrial activity and feature high levels of certain heavy metals. We sequenced 18 shotgun metagenomes through Illumina NovaSeq from samples obtained from three different areas in 2020 and 2021. Taxogenomic analyses demonstrate that these soils harbored equal proportions of archaea and bacteria, with Methanobacteriota, Pseudomonadota, Bacteroidota, Gemmatimonadota, and Balneolota as most abundant phyla. Functions related to the transport of heavy metal outside the cytoplasm are among the most relevant features of the community (i.e., ZntA and CopA enzymes). They seem to be indispensable to avoid the increase of zinc and copper concentration inside the cell. Besides, the archaeal phylum Methanobacteriota is the main arsenic detoxifier within the microbiota although arsenic related genes are widely distributed in the community. Regarding the osmoregulation strategies, “salt-out” mechanism was identified in part of the bacterial population, whereas “salt-in” mechanism was present in both domains, Bacteria and Archaea. De novo biosynthesis of two of the most universal compatible solutes was detected, with predominance of glycine betaine biosynthesis (betAB genes) over ectoine (ectABC genes). Furthermore, doeABCD gene cluster related to the use of ectoine as carbon and energy source was solely identified in Pseudomonadota and Methanobacteriota.

Experimental characterization of contact resistance of desert soil with waste-enhancement materials in grounding systems

This study aims to investigate how the electrical-resistivity of different soils and grounding enhancement-materials is influenced by water-content and the dehydration under ambient and high-constant temperatures. The used enhancement-materials were extracted from surrounding wastes in Egyptian desert. First, waste enhancement-materials, their developed-mixture and soil samples were prepared for experimental testing. The contact-resistance between soil and enhancement-material was measured by using a designed soil-box. It was observed that adding a wood-ash as an interface-layer between the soil and enhancement-materials decreased significantly the contact-resistance. The developed-mixture and wood-ash demonstrated highest water-content retention when compared against the other enhancement-materials. This maintains their resistivity values low to serve for grounding purpose. The wood-ash has the smallest grain–size diameter among the enhancement-materials. This is why it ensures effective contact between the soil and enhancement-materials in grounding systems. The effect of varying injected-current through the soil-box on the resistance values of soils and enhancement-materials is also investigated. Finally, the effectiveness of using of these materials in reducing the resistance-to-ground of a practical copper rod buried into desert soil is evaluated.

Ecological Risk Assessment of Cu, Ni, Cd, Hg, Zn, Pb and As in Typical Farmland Gray-Brown Desert Soil in China

Ecological Risk Assessment of Cu, Ni, Cd, Hg, Zn, Pb and As in Typical Farmland Gray-Brown Desert Soil in China

Desertibaculum subflavum gen. nov., sp. nov., a novel member of the family Sneathiellaceae isolated from the Kumtag Desert soil.

A novel rod-shaped bacterium, designated as strain SYSU D60015T that formed yellowish colonies was isolated from a sandy soil collected from the Kumtag Desert in Xinjiang, China. Cells were Gram-stain-negative, oxidase-positive, catalase-negative and motile with a single polar flagellum. Growth optimum occurred between 28 and 37°C, pH 7.0 and with 0-0.5% (W/V) NaCl. The predominant cellular fatty acids (> 5%) were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C19:0 cyclo ω8c, C18:1 ω7c 11-methyl and C16:0. The polar lipid profile contained one phosphatidylethanolamine, one diphosphatidylglycerol, one phosphatidylglycerol, one unidentified phospholipid, three unidentified aminolipids, two unidentified aminophospholipids and seven unidentified lipids. The only respiratory quinone was ubiquinone-10. Based on 16S rRNA gene sequence phylogenetic analysis, strain SYSU D60015T was found to form a distinct linage within the family Sneathiellaceae, and had 16S rRNA gene sequence similarities of 90.8% to Taonella mepensis H1T, and 90.2% to Ferrovibrio denitrificans S3T. The genome of SYSU D60015T was 5.66 Mb in size with 68.2% of DNA G + C content. The low digital DNA-DNA hybridization (dDDH, 18.0%), average nucleotide identity (ANI, 77.5%) and amino acid identity (AAI, 56.0%) values between SYSU D60015T and Ferrovibrio terrae K5T indicated that SYSU D60015T might represent a distinct genus. Based on the phylogenetic, phenotypic, chemotaxonomic and genomic data, we propose Desertibaculum subflavum gen. nov., sp. nov. as a novel species of a new genus within the family Sneathiellaceae. The type strain is SYSU D60015T (= NBRC 112952T = CGMCC 1.16256T).