Anatomical Description Research Articles

International expert consensus on the surgical anatomic classification of radical hysterectomies

The anatomic descriptions and extents of radical hysterectomy often vary across the literature and operative reports worldwide. The same nomenclature is often used to describe varying procedures, and different nomenclature is often used to describe the same procedure despite the availability of guideline and classification systems. This makes it difficult to interpret retrospective surgical reports, analyze surgical databases, understand technique descriptions, and interpret the findings of surgical studies. In collaboration with international experts in gynecologic oncology, the purpose of this study was to establish a consensus in defining and interpreting the 2017 updated Querleu-Morrow classification of radical hysterectomies. The anatomic templates of type A, B, and C radical hysterectomy were documented through a set of 13 images taken at the time of cadaver dissection. An online survey related to radical hysterectomy nomenclature and definitions or descriptions of the associated procedures was circulated among international experts in radical hysterectomy. A 3-step modified Delphi method was used to establish consensus. Image legends were amended according to the experts' responses and then redistributed as part of a second round of the survey. Consensus was defined by a yes response to a question concerning a specific image. Anyone who responded no to a question was welcome to comment and provide justification. A final set of images and legends were compiled to anatomically illustrate and define or describe a lateral, ventral, and dorsal excision of the tissues surrounding the cervix. In total, there were 13 questions to review, and 29 experts completed the whole process. Final consensus exceeded 90% for all questions except 1 (86%). Questions with relatively lower consensus rates concerned the definitions of types A and B2 radical hysterectomy, which were the main innovations of the 2017 updated version of the 2008 Querleu-Morrow classification. Questions with the highest consensus rates concerned the definitions of types B1 and C, which are the most frequently performed radical hysterectomies. The 2017 version of the Querleu-Morrow classification proved to be a robust tool for defining and describing the extent of radical hysterectomies with a high level of consensus among international experts in gynecologic oncology. Knowledge and implementation of the exact definitions of hysterectomy radicality are imperative in clinical practice and clinical research.

Two new remarkable species of Barbacenia (Velloziaceae) from the Brazilian Espinhaço Range in honor of Renato Mello-Silva

Two new species of Barbacenia (Velloziaceae) from the Brazilian campo rupestre from the southern part of the Espinhaço Range are here described and illustrated. Both new species, Barbacenia glaucescens Ferreira-Junior & Andr.Cabral sp. nov. and B. mellosilvae Andr.Cabral & Ferreira-Junior sp. nov., resemble B. gentianoides Taub. ex Goethart & Henrard, and the second one also resembles B. longiflora Mart. However, both can be differentiated by a unique combination of morphological and anatomical characters. Morphological and anatomical descriptions, illustrations, photographs, phenological information, provisional risk assessments, distribution map, and taxonomic notes are provided for the new species.

Caracterizacion morfológica del paladar duro en los gatos Tabby

The aim of this study was to carry out the morphological characterization of the Tabby cat’s hard palate by light and scanning electron microscopy (SEM), in addition to gross and morphometric analysis. A total of 20 Tabby cat heads used. The materials was provided from the Balıkesir Metropolitan Municipality Street Animals Temporary Nursing Home and Rehabilitation Center, Balıkesir, Turkey. Adult, regardless of gender difference, died from variety of reasons and 20 Tabby cat cadavers which weigh approximately 3–4 kg were brought to the laboratory in accordance with the procedure. Hard palates were subjected to gross examination, morphometric analysis, light and SEM.The Tabby cats hard palate was split up rostral and caudal parts and incisive papilla (papilla incisive), rugae palatine and with a gutter between them, the presence of palatine raphe and secondary rugae palatine were observed. The rostral and caudal parts of the hard palate were determined to have a smooth appearance with SEM 540X magnification and the presence of epithelial desquamations was found out. It was determined that the rostral and caudal part of the Tabby cat’s hard palate had a honeycomb appearance of the microplicae of the 3000X magnification hard palate epithelium with SEM. Round islets were detected with the SEM at the magnification of 600X of the incisive papilla, the 270X of the rostral–caudal parts and the 44X of the gutter between the palatine rugae. These adaptations of the Tabby cat’s hard palate might increase its efficiency during ingestion and mastication of hard food and in directing the food backward. The study shows the detailed anatomical description of the hard palate of the Tabby cat with light and SEM.

A study on the vertebral column of the dice snake Natrix tessellata (Serpentes, Natricidae) from Denizli (western Anatolia, Turkey).

The vertebral anatomy of snakes has attracted the attention of researchers for decades and numerous studies have been made for extinct and extant species. The present study investigated the morphological variations in vertebral structure among different vertebral regions in the dice snake Natrix tessellata, and provides a detailed anatomical and microstructural description of the vertebral column. Vertebrae were analyzed and compared using x-ray imaging, scanning electron microscopy, micro-computed tomography, and histological techniques. The vertebral column of N. tessellata is divided into three regions: precloacal, cloacal, and caudal. Unlike in many other tetrapods and snakes, the atlas of N. tessellata does not form a complete ring. It has a flat and roughly trilobate shape with a prominent middle lobe. The axis has two hypapophyses. The anterior precloacal region of the vertebral column has longer and more paddle-shaped hypapophyses, distinguishing it from the posterior and mid-trunk vertebrae. The anterior cloacal vertebrae have a short hypapophysis rather than a hemal keel, and the lymphapophysis extends outward, curving slightly. The cotyle and condyle of the caudal vertebrae exhibited a closer resemblance to a rounded shape, while the pleurapophysis extended ventrolaterally and curved ventrally near its distal end. Paired hemapophyses were present at the posterior-most point of the centrum instead of a hypapophysis. In light of previous fossil findings, our anatomical comparison of the vertebral and transverse processes indicates that the extant Natrix has a more flexible and less rigid spine than its ancestors. Overall, the vertebral differences among snake anatomical regions or taxa are a testament to the remarkable diversity and adaptability of these fascinating reptiles.

Endoscopic transorbital resection of the temporal lobe: anatomic qualitative and quantitative study.

Starting from an anatomic study describing the possibility of reaching the temporal region through an endoscopic transorbital approach, many clinical reports have now demonstrated the applicability of this strategy when dealing with intra-axial lesions. The study aimed to provide both a qualitative anatomic description of the temporal region, as seen through a transorbital perspective, and a quantitative analysis of the amount of temporal lobe resection achievable via this route. A total of four cadaveric heads (eight sides) were dissected at the Laboratory of Surgical Neuroanatomy (LSNA) of the University of Barcelona, Spain. A stepwise description of the resection of the temporal lobe through a transorbital perspective is provided. Qualitative anatomical descriptions and quantitative analysis of the amount of the resection were evaluated by means of pre- and post-dissection CT and MRI scans, and three-dimensional reconstructions were made by means of BrainLab®Software. The transorbital route gives easy access to the temporal region, without the need for extensive bone removal. The resection of the temporal lobe proceeded in a subpial fashion, mimicking what happens in a surgical scenario. According to our quantitative analysis, the mean volume removed was 51.26%, with the most superior and lateral portion of the temporal lobe being the most difficult to reach. This anatomic study provides qualitative and quantitative details about the resection of the temporal lobe via an endoscopic transorbital approach. Our results showed that the resection of more than half of the temporal lobe is possible through this surgical corridor. While the anterior, inferior, and mesial portions of the temporal lobe were easily accessible, the most superior and lateral segment was more difficult to reach and resect. Our study serves as an integration to the current anatomic knowledge and clinical practice knowledge highlighting and also as a starting point for further anatomic studies addressing more selected segments of the temporal lobe, i.e., the mesial temporal region.

Comparative Anatomical and Histological study of gall bladder in Mallard (Anas platyrhynchos) and Broiler fowl (Gallus gallus )

The current study was investigated anatomical and histological description of gall bladder of two specious which different in nutrition and life habitat Mallard (Anas platyrhnchos) aquatic life bird, and broiler fowl (Gallus gallus) wild life bird ,used ten bird of each specious ,anatomical result founded the gall bladder is located at the visceral surface right lobe of liver at right side of abdominal cavity, and composed of three parts head, neck and body, gall bladder in both specious appeared with dark green color. Gall bladder of mallard has balloon shape more elongated organ while in Gallus gallus is pear shape, the mean weight of gall bladder in mallard (1.91±0.56 g) , length (3.07±0.505 cm) and width (3.07±0.5 cm ) while broiler fowl weight of gall bladder (8.24±2.88 g), length (3.31±0.8 cm)and width (3.56±1.32 cm ) .Histologically; Gall bladder in mallard has three main layers: tunica mucosa, tunica muscularis and tunica adventitia ,tunica mucosa which lined by simple columnar epithelium non-ciliated with goblet cells and the lamina propria-submucosa composed of loos connective tissue , tunica muscularis was a thick layer of muscles (smooth muscles) and adventitia made of loos connective tissue , while gall bladder of Gallus gallus domesticus has three layer; tunica mucosa, lamina propria -submucosa, tunica muscularis and adventitia , tunica mucosa which lined by simple columnar epithelia non- ciliated without goblet cells , with present lamina propria -submucosa fused with lamina propria ,and thin layer of tunica muscularis and adventitia made of loos connective tissue .

FDG-guided excision of peripheral non-palpable lymph nodes in the axilla with suspected malignancy

ObjectiveTo define the feasibility of 18F-fluorodeoxyglucose (FDG) uptake tracing in the excision of potentially malignant non-palpable lymph nodes (NLN), especially in the axilla. Materials and methodsFindings of 493 peripheral lymph node (LN) excisions were examined in the study. Three hundred seventy-eight LNs were palpable and the remaining 115 LNs were non-palpable. The excised NLNs were analyzed in two groups, NLNs excised based on the anatomical descriptions of imaging methods (n=62), image-guided NLN excisions (n=53). Among the image-guided excisions, 27 NLNs were excised using real-time ultrasound (US) guidance, and 26 NLNs were excised using FDG uptake tracing. ResultsImage-guided excisions provided better diagnostic accuracy for potentially malignant NLNs compared with excisions made located based on imaging descriptions (P=0.029). In these image-guided NLN excisions, the FDG uptake-trace method combined with positron emission tomography-computed tomography (PET-CT) was more successful in detecting malignant NLNs compared with US guidance (P=0.04). NLN excisions based on FDG uptake were completed in a shorter time than both excisions with US guidance (P=0.006) and excisions made locating based on imaging definitions (P=0.001). ConclusionPotentially malignant NLN excisions can be challenging for surgeons, and can result in prolonged surgical time. It may be extrapolated from our study that anatomy and metabolism-based LN excision guidance using information obtained in PET-CT and following FDG uptake tracing may improve the accuracy of histologic examinations, and thus increase the probability of achieving a correct diagnosis.

Detail Study of Sandhi Sharir with special reference to Jaanu Sandhi

According to modern text joint is a place where two or more bones are articulates with each other. Aacharya Sushruta has quoted that although there are numerous Sandhi in our body which cannot be counted so only Asthi Sandhi should be considered while enumerating Sandhi. Total two hundred and ten Sandhi are present in human body. Every bone is unite by joints and covered by muscles and gets blood supply by different Sira and Dhamani. These bones unite to form Asthi Sandhi. In Ayurveda the main Sthana of Kapha Dosha is all joints in human body. Janu Sandhi (knee) is the largest and most complex synovial joint of the body and is of great importance because it plays great role in movements like walking and stability, including maintenance of the erect posture and the transmission of the body weight. Sandhi can be taken as union of two or more bone in Ayurvedic Samhitas the description of anatomy of Sandhi in detail is not found. It is observed that the incidence of joints disorders is increasing day by day. It is burning problem for families and society. A thorough knowledge of the structures and functions of the joint is required to diagnose and treat the disease of joints.

Essential neural anatomy for creating a clinically translatable osseointegrated neural interface for prosthetic control in sheep

Aim: Ovine models for osseointegrated prosthetics research are well established, but do not consider neural control of advanced prostheses. The validity of interfacing technologies, such as the Osseointegrated Neural Interface (ONI), in their ability to provide communication between native nerves and advanced prosthetics is required, necessitating a stable, longitudinal large animal model for testing. The objective of this study is to provide a detailed anatomic description of the major nerves distal to the carpal and tarsal joints, informing the creation of a chronic ONI for prosthetic control in sheep. Methods: Six pelvic and six thoracic cadaveric limbs from mature female, non-lactating sheep were utilized. Radiographs were obtained to determine average bone length, medullary canal diameter, and cortical bone thickness. Microsurgical dissection was performed to discern topographical neuroanatomy and average circumferences of the major nerves of the pelvic and thoracic limbs. Histologic analysis was performed. A surgical approach for the creation of ONI was designed. Results: Average metacarpal and metatarsal length was 15.0 cm (± 0.0) and 19.7 cm (± 1.0), respectively. Average intramedullary canal diameter was 12.91 mm (± 3.69) for forelimbs and 12.60 mm (± 3.69) for hindlimbs. The thoracic limb nerves consisted of one dorsal and three ventral nerves, with an average circumference of 5.14 mm (± 2.00) and 5.05 mm (± 1.06), respectively. Pelvic limb nerves consisted of two dorsal and one ventral nerve with an average circumference of 6.27 mm (± 1.79) and 5.40 mm (± 0.53), respectively. Conclusions: These anatomic data inform the surgical approach and manufacture of a sensory ONI for chronic testing in awake, freely ambulating animals for future clinical translation.

The morphological, anatomical, palynological, seed micromorphological studies and its taxonomical significance in Delphinium turcicum (Ranunculaceae), a local endemic spurless species for Turkey

In this study, species of Delphinium turcicum (Ranunculaceae), which is endemic to Türkiye, for its morphological, anatomical, palynological, micromorphological and taxonomical significance was investigated. Additionally, Delphinium venolusum, which is a similar species of Delphinium turcicum, was studied. Anatomic structure descriptions of these species and seed descriptions of Delphinium turcicum were given for the first time in this work. In addition, the comparison of these species contributes to the solution of taxonomic problems. Features of anatomic structures (root, stem, and leaf sections) were shown. Also, these structures were discussed taxonomic significance. The similarities and differences between D. turcicum and D. venulosum were revealed in this study. In palynological investigations; polar, equatorial, exine, intine, colpus length and width were measured. Polar/Equatorial (P/E) ratio and pollen ornamentation were determined. The pollen grains of these two species are spherical and isopolar symmetrical. Pollen types have determined as monad for D. turcicum and D. venulosum species. Pollen aperture types are tricolpate. Pollen exine ornamentations are microechinate. In this work photographs of pollen and seed were taken by using light microscopy and scanning electron microscopy (SEM). Seed micromorphological characteristics were given for both species. Seeds are subglobose and have continuous rings of transversal squamulose for both species. Seed ornamentations have continuous rings of transversal squamulose and generally erect papillae.

Management of Perianal Fistulizing Crohn's Disease.

Perianal fistulizing Crohn's disease (CD) represents a severe phenotype of CD that is associated with significant morbidity and reduction in quality of life. Perianal fistulizing CD is caused by a complex interplay of genetic predisposition, immune dysregulation, gut dysbiosis, and various unknown physiological and mechanical factors. A multidisciplinary approach is hence required for optimal management . A detailed anatomical description and classification of perianal fistula, including comprehensive clinical, endoscopic, and radiological diagnostic workup, is an important prerequisite to treatment. For simple perianal fistulas, use of antibiotics and immunomodulators, with or without fistulotomy, are appropriate measures. The medical management of complex perianal fistula, on the other hand, requires adequate control of infection before initiation of therapy with immunomodulators. In active complex perianal fistula, anti-tumor necrosis factors remain the most accepted therapy, with concomitant use of antibiotics or immunomodulators enhancing the efficacy. For patients refractory to anti-tumor necrosis factors, treatment with anti-integrins, anti-interleukins, and small molecules is being evaluated. Mesenchymal stem cells, hyperbaric oxygen therapy, and exclusive enteral nutrition have also been investigated as adjunct therapies. Despite the expansion of the medical armamentarium, a large proportion of the patients require surgical interventions. In this review, we provide an up-to-date overview of the pathophysiology, clinical presentation, diagnosis, and medical management of perianal fistulizing CD. A brief overview of the surgical management of perianal fistulizing CD is also provided.

Transorbital Microsurgery: An Anatomical Description of a Minimally Invasive Corridor to the Anterior Cranial Fossa and Paramedian Structures.

Objectives Transorbital neuroendoscopic surgery (TONES) has ignited interest in the transorbital access corridor, increasing its use for single and multi-portal skull base interventions. However, the crowding of a small corridor and two-dimensional viewing restrict this access portal. Design Cadaveric qualitative study to assess the feasibility of transorbital microsurgery (TMS). Setting Anatomical dissection steps and instrumentation were recorded for homogeneous methodology. Participants Six cadaveric specimens were systematically dissected using TMS to the anterior cranial fossa and paramedian structures. Main Outcome Measures Anatomical parameters of the TMS craniectomy were established, and the visible and accessible neuroanatomy was highlighted. Results A superior lid crease incision achieved essential orbital rim exposure and preseptal dissection. The orbital roof craniectomy is defined by three boundaries: (1) frontozygomatic suture to the frontosphenoid suture, (2) frontal sinus and cribriform plate, and (3) frontal sinus and orbital rim. The mean (standard deviation) craniectomy was 440 mm 2 (78 mm 2 ). Exposing the ipsilateral optic nerve and internal carotid artery obviated the need for frontal lobe retraction to identify the A1-M1 bifurcation as well as near-complete visualization of the M1 artery. Conclusion TMS is a feasible corridor for intracranial access. Mobilization of orbital contents is imperative for maximal intracranial access and protection of the globe. TMS enables access to the frontal lobe base, ipsilateral optic nerve, and most of the ipsilateral anterior circulation. This cosmetically satisfactory approach causes minimal destruction of the anterior skull base with satisfactory exposure of the anterior cranial fossa floor without sinus invasion.

Retroperitoneoscopy of the presacral space in horses: Surgical access and anatomy.

Surgical approaches to the equine rectum and perirectal area are described in the literature. However, surgeries in this region can be challenging. To describe the surgical anatomy of the presacral space and to evaluate its access using a retroperitoneoscopic approach. Ex vivo experiment. Preliminary dissections were performed in two cadavers to define the boundaries of the presacral space and to determine portal locations for the surgical approach. After that, nine cadavers were used for experimental presacral retroperitoneoscopic procedure in a standing position. Following retroperitoneoscopy, cadavers were dissected to confirm the anatomical structures observed during the endoscopic procedures, to control the location of each portal and to record iatrogenic trauma. The presacral space was bordered by the vertebral column from the ventral aspect of lumbosacral promontorium to the first coccygeal vertebra dorsally and by the presacral fascia and peritoneum ventrally. Lateral limits were composed of the sacrosciatic ligament and transversalis fascia. Cranial and caudal borders were composed of the peritoneum and coccygeal and levator ani muscles respectively. Retroperitoneoscopic portals were placed between the external anal sphincter and semimembranosus muscles and between the base of the tail and the external anal sphincter muscle through the anococcygeal fascia to enter the space by its caudal border. The retroperitoneal space was reached in all cases and the dorsal and lateral aspects of the rectum were visualised after creation of a working space. Use of cadaver specimens do not permit to evaluate the tolerance in living animals and the surgical complications such as rectal damage, haemorrhage and infection. This study provides an anatomical description and surgical access of the presacral space with a minimal invasive approach. Retroperitoneoscopy allows access to the rectum and the dorsal aspect of the pelvis.

State of the heart: Anatomical annotation and assessment of morphological cardiac variation in Atlantic salmon (Salmo salar L.)

Farmed Atlantic salmon suffer from deviating cardiac morphology, likely compromising fish health and robustness. To mitigate the potentially devastating consequences of morphological deviations of the heart, the cause and consequences of these abnormalities need to be elucidated. This endeavour is, however, complicated by several obstacles. First, a detailed nomenclature of gross anatomical structures of the pyramidal fish heart is needed to develop standardized descriptions of morphological deviations. Second, standardized methods and scoring systems for quantitative and qualitative descriptions of cardiac anatomy are lacking. Lastly, the normal morphological development of the heart through the life cycle of wild specimens has not been described, making it challenging to identify deviating morphology at different life stages of farmed salmon. Here, we develop a nomenclature for the exterior of a pyramidal teleost heart, using the Atlantic salmon heart as a model. Further, we have unified and described the most common quantitative methods used for measuring heart morphology and developed a qualitative methodology to detect morphological abnormalities not identified by the quantitative methods. We describe the methodologies' applicability and assess the qualitative method's robustness. Using quantitative measures, heart morphology in the wild and farmed salmon is compared throughout their life cycles. Further, we show that the qualitative method is superior to the quantitative method in detecting traits more prevalent in deceased fish after exposure to stressful routine field treatments, illustrating the complementary nature of these two methods.

Morphological study of the anatomical variations of anterior belly of digastric muscle in Brazilian cadavers.

Cases of variations in anterior belly of the digastric muscle must be carefully identified to avoid misinterpretations and assist in the correct surgical or aesthetic procedure and help in the teaching of anatomy. The aim of this study was to describe the anatomical variations of anterior belly of digastric muscle in Brazilian cadavers. Thirty-one human heads were selected, from adult cadavers (18-80 years, 29 males and 2 females). The morphology of the anterior belly of the digastric muscle was observed, identifying the possible anatomical variations that were characterised and classified according to the amount of muscle bellies, fibre direction and place of origin and insertion. The morphometric measurements were performed using a digital calliper. To analyse the data obtained, photographic documentation, anatomical description and individual morphometric description of each muscle belly were performed. The incidence of anatomical variation was obtained in percentage (%). The anatomical variation of the anterior belly of the digastric muscle was present in 6 cadavers (19.31%; 1 female and 5 male). All anatomical variations presented an accessory belly to the anterior belly. However, these accessory bellies were configured differently in the location, direction of muscle fibres and in their dimensions (length and width). The gross anatomy of the anterior belly of the digastric muscle and their variations is important to assist in surgical procedures, pathological or diagnostic function. In addition, asymmetrical variations in the submental region must be carefully identified to avoid misinterpretations.

Functional anatomy of mystacial active sensing in rats.

Rats' whisking motion and objects' palpation produce tactile signals sensed by mechanoreceptors at the vibrissal follicles. Rats adjust their whisking patterns to target information type, flow, and resolution, adapting to their behavioral needs and the changing environment. This coordination requires control over the activity of the mystacial pad's intrinsic and extrinsic muscles. Studies have relied on muscle recording and stimulation techniques to describe the roles of individual muscles. However, these methods lack the resolution to isolate the mystacial pad's small and compactly arranged muscles. Thus, we propose functional anatomy as a complementary approach for studying the individual and coordinated effects of the mystacial pad muscles on vibrissae movements. Our functional analysis addresses the kinematic measurements of whisking motion patterns recorded in freely exploring rats. Combined with anatomical descriptions of muscles and fascia elements of the mystacial pad in situ, we found: (1) the contributions of individual mystacial pad muscles to the different whisking motion patterns; (2) active touch by microvibrissae, and its underlying mechanism; and (3) dynamic position changes of the vibrissae pivot point, as determined by the movements of the corium and subcapsular fibrous mat. Finally, we hypothesize that each of the rat mystacial pad muscles is specialized for a particular function in a way that matches the architecture of the fascial structures. Consistent with biotensegrity principles, the muscles and fascia form a network of structural support and continuous tension that determine the arrangement and motion of the embedded individual follicles.

The Auriculotemporal Nerve and TMJ region: anatomy and function

The studies on the auriculotemporal nerve in humans are limited. However, we considered crucial to investigate the topographic relation between the auriculotemporal nerve and the TMJ region that can explain some of the symptoms in TMDs. The results derived from our experience in the anatomic dissection on 16 adult cadaveric heads were compared with what literature describes from 1971 to 2023. The results confirm the existence of a particular anatomic position of the ATN within the infratemporal fossa in direct contact with the lateral pterygoid muscle and the TMJ capsular region. Therefore, there is evidence of a potential entrapment mechanism involving the ATN caused by an internal derangement of TMJ or a spastic condition of the lateral pterygoid muscle. Through a detailed anatomical description of the ATN the present study aims to offer an explanation to the main sensory and otological symptoms that patients with TMJ disorders often complaint, from facial pain and paresthesias to external ear pruritus.

Mapping the macrostructure and microstructure of the in vivo human hippocampus using diffusion MRI.

The hippocampus is classically divided into mesoscopic subfields which contain varying microstructure that contribute to their unique functional roles. It has been challenging to characterize this microstructure with current magnetic resonance based neuroimaging techniques. In this work, we used diffusion magnetic resonance imaging (dMRI) and a novel surface-based approach in the hippocampus which revealed distinct microstructural distributions of neurite density and dispersion, T1w/T2w ratio as a proxy for myelin content, fractional anisotropy, and mean diffusivity. We used the neurite orientation dispersion and density imaging (NODDI) model optimized for grey matter diffusivity to characterize neurite density and dispersion. We found that neurite dispersion was highest in the cornu ammonis (CA) 1 and subiculum subfields which likely captures the large heterogeneity of tangential and radial fibres, such as the Schaffer collaterals, perforant path, and pyramidal neurons. Neurite density and T1w/T2w were highest in the subiculum and CA3 and lowest in CA1, which may reflect known myeloarchitectonic differences between these subfields. Using a simple logistic regression model, we showed that neurite density, dispersion, and T1w/T2w measures were separable across the subfields, suggesting that they may be sensitive to the known variability in subfield cyto- and myeloarchitecture. We report macrostructural measures of gyrification, thickness, and curvature that were in line with ex vivo descriptions of hippocampal anatomy. We employed a multivariate orthogonal projective non-negative matrix factorization (OPNNMF) approach to capture co-varying regions of macro- and microstructure across the hippocampus. The clusters were highly variable along the medial-lateral (proximal-distal) direction, likely reflecting known differences in morphology, cytoarchitectonic profiles, and connectivity. Finally, we show that by examining the main direction of diffusion relative to canonical hippocampal axes, we could identify regions with stereotyped microstructural orientations that may map onto specific fibre pathways, such as the Schaffer collaterals, perforant path, fimbria, and alveus. These results highlight the value of combining in vivo dMRI with computational approaches for capturing hippocampal microstructure, which may provide useful features for understanding cognition and for diagnosis of disease states.

Anatomical considerations of US-guided carpal tunnel release in daily clinical practice.

Carpal tunnel syndrome is the most frequent compression neuropathy with an incidence of one to three subjects per thousand. As specific anatomical variations might lead to unintended damage during surgical interventions, we present a review to elucidate the anatomical variability of the carpal tunnel region with important considerations for daily clinical practice: several variants of the median nerve branches in and around the transverse carpal ligament are typical and must - similarly to the variant courses of the median artery, which may be found eccentric ulnar to the median nerve - be taken into account in any interventional therapy at the carpal tunnel. Unintended interference in these structures might lead to heavy arterial bleeding and, in consequence, even underperfusion of segments of the median nerve or, if neural structures such as variant nerve branches are impaired or even cut, severe pain-syndromes with a profound impact on the quality of life. This knowledge is thus crucial for outcome- and safety-optimization of different surgical procedures at the volar aspect of the wrist and surgical therapy of the carpal tunnel syndrome e.g., US-guided carpal tunnel release, as injury might result in dysfunction and/or pain on wrist motion or direct impact in the region concerned. For most variations, anatomical and surgical descriptions vary, as official classifications are still lacking.

Culm anatomy of the genus Otatea (Poaceae, Bambusoideae, Bambuseae, Guaduinae) as a contribution to Mexican species identification

Otatea is a Neotropical woody bamboo genus with thirteen described species. It is distributed from Mexico to Central America and northeastern Colombia. In Mexico, the archaeological evidence suggests that the culms of Otatea have been used for centuries by some ethnic groups. It is important to explore morphological and anatomical characters for species identification in woody bamboos as consequence of its monocarpic nature. Most studies on culm anatomy are centered on commercially used species describing their physical and mechanical properties. The aim of this work was to find culm anatomical characters with taxonomic value. To perform this, we collected internode samples from adult plants at mid culm of 15 specimens that represent 10 described species and two putative new species of Otatea. Our results include descriptions of culm anatomy at a cross section accompanied with images that detail all the structures measured and described. Additionally, we elaborate a key based on anatomical characters. This contribution is the first to explore culm anatomy of the genus Otatea in search for characters with taxonomic value, providing useful evidence for species identification, including samples from archaeological sites.