Schiff Base Derivatives Research Articles

Spectral, crystal structure of novel Pyran-2-one Zwitterion Schiff derivative: Thermal, Physicochemical, DFT/HSA-interactions, enol↔imine tautomerization and anticancer activity

A novel Schiff base (SB) derivative, (E)-3-(1-((2,6-diisopropylphenyl)-imino)-ethyl)-4‑hydroxy-6-methyl-2H-pyran-2-one, was synthesized in a high yield through the direct condensation of 2,6-diisopropylaniline with 3-acetyl-4‑hydroxy-6-methyl-2H-pyran-2-one in ethanol under reflux conditions. The synthesized ligand was identified using different elemental analysis (CHN-EA) and spectroscopic techniques (2D-nuclear magnetic resonance (1H- & 13C- NMR), Fourier transform infrared (FT-IR) and mass (MS) spectroscopies), and their structural analysis was performed by single crystal X-ray diffraction (XRD). In addition, the thermogravimetric behavior of the ligand was evaluated using thermogravimetric analysis (TGA) and differential thermal analysis (DTA). The DFT calculations on the gaseous state and in chloroform as solvent using B3LYP-D3/6–311++G(2d,p) and the HSA calculations were performed to examine the stability of the tautomeric forms of the ligand and to understand its Zwitterionic behaviors. The results showed that the ligand exhibits a zwitterionic form stabilized by an intramolecular single proton transfer process between the enol and imine tautomerism. The enol↔imine tautomerization was determined by XRD and computed by DFT/HSA calculations, which proved the stabilization of the [N+−−H⋯O−−] zwitterionic form via S6 intra-H-bond. In addition, the Colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the ligand exhibits high activity against cancer cells, specifically HeLa cells, at low concentrations.

Unveiling potent Schiff base derivatives with selective xanthine oxidase inhibition: In silico and in vitro approach

This research describes the synthesis by an environmentally-friendly method, microwave irradiation, development and analysis of three novel and one previously identified Schiff base derivative as a potential inhibitor of bovine xanthine oxidase (BXO), a key enzyme implicated in the progression of gout. Meticulous experimentation revealed that these compounds (10, 9, 4, and 7) have noteworthy inhibitory effects on BXO, with IC50 values ranging from 149.56 µM to 263.60 µM, indicating their good efficacy compared to that of the standard control. The validation of these results was further enhanced through comprehensive in silico studies, which revealed the pivotal interactions between the inhibitors and the catalytic sites of BXO, with a particular emphasis on the imine group (-C = N-) functionalities. Intriguingly, the compounds exhibiting the highest inhibition rates also showcase advantageous ADMET profiles, alongside encouraging initial assessments via PASS, hinting at their broad-spectrum potential. The implications of these findings are profound, suggesting that these Schiff base derivatives not only offer a new vantage point for the inhibition of BXO but also hold considerable promise as innovative therapeutic agents in the management and treatment of gout, marking a significant leap forward in the quest for more effective gout interventions.

Record-Efficiency Printable Hole-Conductor-Free Mesoscopic Perovskite Solar Cells Enabled by the Multifunctional Schiff Base Derivative.

Tailoring multifunctional additives for performing interfacial modifications, improving crystallization, and passivating defects is instrumental for the fabrication of efficient and stable perovskite solar cells (PSCs). Here, a Schiff base derivative, (chloromethylene) dimethyliminium chloride (CDCl), is introduced as an additive to modify the interface between the mesoporous TiO2 electron transport layer and the MAPbI3 light absorber during the annealing process. CDCl chemically links to TiO2 and MAPbI3 through coordination and hydrogen bonding, respectively, and results in the construction of fast electron extraction channels. CDCl also optimizes the energy-level alignment of the TiO2/MAPbI3 heterojunction and improves the pore-filling and crystallization of MAPbI3 in the mesoscopic scaffold, which inhibits nonradiative recombination and eliminates open-circuit voltage losses. As a result, an impressive power conversion efficiency of 19.74%, which is the best one ever reported, is obtained for printable carbon-based hole-conductor-free PSCs based on MAPbI3.

Eco-friendly green synthesis of N‑pyrazole amino chitosan using PEG-400 as an anticancer agent against gastric cancer cells via inhibiting EGFR.

The present study was conducted to develop a green process that provides access to the development of Schiff base derivatives of chitosan with the heterocyclic moiety as a novel class of anti-gastric cancer agent. In the present study, we have synthesized these derivatives by reacting various pyrazoles with chitosan using CAN in PEG400. The compounds were synthesized in 20min in excellent yield by using CAN at 5% in PEG400 at 80°C in the shortest reaction time of 20min. The PEG400 could be efficiently recycled for the three consecutive runs. The developed compounds were tested for EGFR-TK inhibition using a Kinase-Glo Plus luminescence kinase assay kit where they exhibited significant activity revealing compound 2d as the most potent analog, while other compounds showed mild to moderate inhibitory activity. MTT assay was conducted to determine the effect of the three most potent EGFR inhibitors (2b, 2c, and 2d) on the proliferation of gastric cancer cells (SGC-7901). The results showed compound 2d as the most potent anticancer agent against SGC7901 cells. The effect of compound 2d was also quantified on the apoptosis and cell phase of SGC7901 cells using flow cytometry assay at various concentrations ranging from 0, 10, 20, and 30µM. Results suggest that compound 2d showed significant inhibition of SGC-7901 by inducing apoptosis and arresting G0/G1 cell phase. The western blot analysis also revealed that compound 2d significantly inhibited the overexpression of EGFR in SGC-7901 cells. The study successfully demonstrated the development of N‑pyrazole amino chitosan as a novel class of agent against gastric cancer via inhibition of EGFR.

Critical view on antimicrobial, antibiofilm and cytotoxic activities of quinazolin-4(3H)-one derived schiff bases and their Cu(II) complexes

A series of nine 2,3-disubstituted-quinazolin-4(3H)-one derived Schiff bases and their three Cu(II) complexes was prepared and tested for their antimicrobial activities against reference strains Staphylococcus aureus ATCC 29213 and Enterococcus faecalis ATCC 29212 and resistant clinical isolates of methicillin-resistant S. aureus (MRSA) and vancomycin-resistant E. faecalis (VRE). All the substances were tested in vitro against Mycobacterium tuberculosis H37Ra ATCC 25177, M. kansasii DSM 44162 and M. smegmatis ATCC 700084. While anti-enterococcal and antimycobacterial activities were insignificant, 3-[(E)-(2-hydroxy-5-nitrobenzylidene)amino]-2-(2-hydroxy-5-nitrophenyl)-2,3-dihydroquinazolin-4(1H)-one (SB3) and its Cu(II) complex (SB3–Cu) demonstrated bacteriostatic antistaphylococcal activity. In addition, both compounds, as well as the other two prepared complexes, showed antibiofilm activity, which resulted in a reduction of biofilm formation and eradication of mature S. aureus biofilm by 80% even at concentrations lower than the values of their minimum inhibitory concentrations. In addition, the compounds were tested for their cytotoxic effect on the human monocytic leukemia cell line THP-1. The antileukemic efficiency was improved by the preparation of Cu(II) complexes from the corresponding non-chelated Schiff base ligands.

A quinoline derived Schiff base as highly selective ‘turn-on’ probe for fluorogenic recognition of Al3+ ion

A designed quinoline-derived Schiff base QnSb has been synthesized for fluorescent and colorimetric recognition of Al3+ ions in a semi-aqueous medium. The compound QnSb has been characterized by elemental analysis, FT-IR, 1H/13C NMR, UV–Vis and fluorescence spectral techniques. The crystal structure of the QnSb was confirmed by single crystal X-ray diffraction (SC-XRD) analysis. Notably, almost non-fluorescent QnSb served as a ‘turn on’ responsive probe for Al3+ by inducing a remarkable fluorescence enhancement at 422 nm when excited at 310 nm. The probe QnSb exhibited high selectivity for Al3+ in CH3CN/H2O (4:1, v/v) solution over several competing metal ions (e.g., Mg2+, Pb2+, Zn2+, Cd2+, Co2+, Cu2+, Ca2+, Ni2+, Fe3+/2+, Cr3+, Mn2+, Sn2+, and Hg2+). The limit of detection (LoD) was computed as low as 15.8 nM which is significantly lower than the permissible limit set by WHO for Al3+ ions in drinking water. A 1:1 binding stoichiometry of complex QnSb-Al3+ was established with the help of Job’s plot, ESI-MS, NMR and DFT analyses. Based on its remarkable sensing ability, the probe QnSb was utilized to establish molecular logic gates, and the fluorescence detection of Al3+ could clearly be demonstrated on the filter paper test strips.

New benzimidazole based Schiff bases as potent anti-alzheimer agents: Synthesis, bio-evaluation and molecular docking study

In search of potent anti-Alzheimer agent benzimidazole based Schiff base derivatives (1–18) were synthesized and evaluated as dual inhibitor for acetylcholinesterase and butyrylcholinesterase enzymes. All analogs among the series except analog 1 and 16 showed a variable degree of inhibitory activity with IC50 value ranging between 0.10 ± 0.01 to 12.40 ± 0.30 µM for acetylcholinesterase and 0.20 ± 0.01 to 11.10 ± 0.30 µM for butyrylcholinesterase. The most potent analog found among the series was analog 8 having IC50 value 0.10 ± 0.01 and 0.20 ± 0.01 µM for both acetylcholinesterase and butyrylcholinesterase inhibition respectively. The structures of all synthesized analogs were confirmed through NMR and HR-EIMS. Structure activity relationship (SAR) has been established for all newly synthesized derivatives. To understand the binding interaction of most active derivatives with enzyme active site, molecular docking study were performed. The toxicity and mutagenicity of compound 8 was predicted using in silico software, namely Derek Nexus® (version 6.3). Various toxicity endpoints, including chromosomal damage, skin sensitization, hepatotoxicity were predicted. The degradation profile of compound 8 was predicted in silico by Zeneth software (version 9.0.1) resulting in a probability of the formation of seven potential degradation products.

New Schiff's base derivative and their nano‐sized Cr (III), Fe (III), Ru (III), and Ir (III) complexes: Preparation, DFT, characterization, bio‐catalytic, DNA interactions, cytotoxicity, and docking studies

Nano‐sized trivalent metal complexes of Cr (III), Fe (III), Ru (III), and Ir (III) were synthesized and characterized of the form [M (FMAVIEP)Cl2] where FMAVIEP = ligand (C14H11N2O2) and M = Cr (III), Fe (III), Ru (III), and Ir (III). Several spectral techniques studies were used to assess our trivalent metal complexes. The trivalent metal complexes' XRD results revealed sharp and intense diffraction peaks, signifying their crystalline properties at nanoscale particle size. Further evidence was obtained from images captured through techniques such as SEM, TEM, energy‐dispersive X‐ray, and AFM. These images confirmed the homogeneous distribution of the trivalent metal complexes over the surface of the complex. DFT studies were used to study the nano‐sized metal (III) complexes via the DFT\B3LYP computational approach, employing a 6–311G* correlation consistent basis set. The energy gap of synthesized complexes was inspected. The obtained data exhibited a strong correlation with the experimental ones, implying the bio‐efficiency of the Ru (III) complex. The absorption measurements for the nano‐sized complexes' interaction with CT‐DNA signify that nano‐sized metal (III) complexes bind through an intercalation mechanism. This conclusion is supported by the observed intrinsic binding constant (Kb) 3.33 × 105–5.84 × 105 M−1.

Synthesis of novel hydrazide Schiff bases with anti-diabetic and anti-hyperlipidemic effects: in-vitro, in-vivo and in-silico approaches

The increasing global incidence of non-insulin-dependent diabetes mellitus (NIDDM) necessitates innovative therapeutic solutions. This study focuses on the design, synthesis and biological evaluation of Schiff base derivatives from 2-bromo-2-(2-chlorophenyl) acetic acid, particularly hydrazone compounds 4a and 4b. Both in-vitro and in-vivo assays demonstrate these derivatives’ strong antidiabetic and anti-hyperlipidemic properties. In a 15-d experiment, we administered 4a and 4b at doses of 2.5 and 5 mg/kg body weight, which effectively improved symptoms of alloxan-induced diabetes in mice. These symptoms included weight loss, increased water consumption and high blood glucose levels. The compounds also normalized abnormal levels of total cholesterol (TC), triacylglycerol (TG) and low-density lipoprotein cholesterol (LDL-C), while raising the levels of high-density lipoprotein cholesterol (HDLC). Computational analysis showed that these compounds effectively inhibited the α-glucosidase enzyme by interacting with key catalytic residues, specifically Asp214 and Asp349. These computational results were confirmed through in-vitro tests, where 4a and 4b showed strong α-glucosidase inhibitory activity, with IC50 values of 0.70 ± 0.11 and 10.29 ± 0.30 µM, respectively. These compounds were more effective than the standard drug, acarbose, which had an IC50 value of 873.34 ± 1.67 µM. Mechanistic studies further indicated competitive inhibition, reinforcing the therapeutic potential of 4a and 4b for NIDDM treatment. Communicated by Ramaswamy H. Sarma

Ratiometric Fluorescence and Chromogenic Probe for Trace Detection of Selected Transition Metals.

The design and development of a fluorescence sensor aimed at detecting and quantifying trace amounts of toxic transition metal ions within environmental, biological, and aquatic samples has garnered significant attention from diagnostic and testing laboratories, driven by the imperative to mitigate the health risks associated with these contaminants. In this context, we present the utilization of a heterocyclic symmetrical Schiff Base derivative for the purpose of fluorogenic and chromogenic detection of Co2+, Cu2+ and Hg2+ ions. The characterization of the ligand involved a comprehensive array of techniques, including physical assessments, optical analyses, NMR, FT-IR, and mass spectrometric examinations. The mechanism of ligand-metal complexation was elucidated through the utilization of photophysical parameters and FT-IR spectroscopic analysis, both before and after the interaction between the ligand and the metal salt solution. The pronounced alterations observed in absorption and fluorescence spectra, along with the distinctive chromogenic changes, following treatment with Co2+, Cu2+ and Hg2+, affirm the successful formation of complexes between the ligands and the treated metal ions. Notably, the receptor's complexation response exhibited selectivity towards Co(II), Cu(II), and Hg(II), with no observed chromogenic changes, spectral variations, or band shifts for the various tested metal ions, including Na+, Ag+, Ni2+, Mn2+, Pd2+, Pb2+, Cd2+, Zn2+, Sn2+, Fe2+, Fe3+, Cr3+ and Al3+. This absence of interaction between these metal ions and the ligand could be attributed to their compact or inadequately conducive conduction bands for complexation with the ligand's structural composition. To quantify the sensor's efficacy, fluorescence titration spectra were employed to determine the detection limits for Co2+, Cu2+ and Hg2+, yielding values of 2.92 × 10-8, 8.91 × 10-8, and 4.39 × 10-3 M, respectively. The Benesi-Hildebrand plots provided association constant values for the ligand-cobalt, ligand-copper, and ligand-mercury complexes as 0.74, 2.52, and 13.89 M-1, respectively.

A One‐Pot Synthesized Chemodosimetric Chemosensor for Highly Sensitive Cyanide Ion Detection in Aqueous Media and from Tobacco Smoke

AbstractA sustainable future world consistently attributes to the development of eco‐friendly methods and materials for detection and elimination of toxic substances from the environment. While addressing the issues, pyrazole carbonitrile derived Schiff base PYR−TZ has been designed and employed for colorimetric and turn on fluorometric cyanide ion sensing. PYR−TZ demonstrated high cyanide ion (CN−) sensitivity with a 1 : 1 binding stoichiometry in aqueous solution. By preventing intramolecular charge transfer, the PYR−TZ sensor demonstrated excellent selectivity towards CN, which caused a color transition from colorless to yellow and impressive “turn‐on” fluorescence emission. The detection thresholds for CN− are 6.5 nm for absorbance spectra and 0.396 nm emission spectra, respectively. Under typical ecological and physiological settings (pH 1–12), the PYR−TZ sensor can function as an effective chemical sensor for detecting the CN− ions. Additionally, the sensor can find CN in tobacco commercial products like cigarettes and cigars. PYR−TZ is a great cyanide sensor probe that has a number of benefits, which includes simple synthesis, distinct color and emission change, a high degree of selectivity, lesser limit of detection, reversibility, and good anti‐interference ability to analyze solution and tobacco commercial things, together with fluorescence imaging. The theoretical optimized structure and electronic transitions were confirmed by DFT and TDDFT studies. On the basis of theoretical and experimental calculations (DFT), the mechanism of the metal ion complex is postulated.

Design, spectral, antibacterial and in-silico studies of new thiosemicarbazones and semicarbazones derived from symmetrical chalcones

A series of new Schiff bases of semicarbazones and thiosemicarbazones have been derived from the condensation of symmetrical chalcones with semicarbazide and thiosemicarbazide, respectively. Various physicochemical and spectroscopic approaches (UV, IR, 1H, and 13C NMR) were used to fully describe the newly synthesized compounds. Schiff base derivatives were screened against bacterial strains viz. Bacillus Subtilis and Escherichia Coli. The DFT approach was also used to analyze some sensitivity descriptors alike chemical potential (µ), electronegativity (χ), hardness (η), and electrophilicity index (ω). Molecular docking was used to determine binding affinity of compounds with the desired protein. Compounds were identified to be more effective against Bacillus Subtilis than E. Coli. The molecular docking calculations of compound (6) exhibited the escalated binding affinity of -8.01 kcal/mol and binds with the active amino acids (Glu57 and Asp162). The prediction of in silico ADME properties discovered that synthesized molecules acquire significant drug-likeness properties.

Synthesis, docking and biological evaluation of some novel [1,2,4] triazolo [5,1-b] quinazoline Schiff base derivatives

Synthesis, docking and biological evaluation of some novel [1,2,4] triazolo [5,1-b] quinazoline Schiff base derivatives

L-alanine Derived Schiff Base Ligated Vanadium(IV) Complex with Phenanthroline as Co-ligand: Synthesis, Crystal Structure and Hirshfeld Surface Analysis

L-alanine Derived Schiff Base Ligated Vanadium(IV) Complex with Phenanthroline as Co-ligand: Synthesis, Crystal Structure and Hirshfeld Surface Analysis

Synthesis, Identification, Biological Evaluation of Seven-membered Heterocyclic Derivatives from 2,3-di Chloroaniline

This study?s focus was centered on the synthesis of heterocyclic compounds incorporating a seven-membered ring, specifically 1,3-oxazepine. The initial phase of the synthesis involved the preparation of azo compound (1) through the coupling of the diazonium salt of 2,3-dichloroaniline with 4-bromoacetophenone in an alkaline alcoholic medium. This was then succeeded by the reaction of azo compound (1) with 4-nitroaniline and 4-methoxyaniline in absolute ethanol, employing glacial acetic acid as a catalyst, resulting in Schiff base derivatives (2) and (3), respectively. Subsequent to this, Schiff base (2) was reacted with phthalic anhydride and maleic anhydride, as well as succinic anhydride, in a dehydrated benzene environment, leading to the formation of heterocyclic seven-membered compounds (4), (5), and (6), respectively. Finally, Schiff base (3) was subjected to reaction with phthalic anhydride and maleic anhydride, as well as succinic anhydride, in dry benzene, resulting in the synthesis of heterocyclic seven-membered compounds (7), (8), and (9) respectively. Each of these synthesized compounds underwent characterization through Fourier-transform infrared and proton nuclear magnetic resonance spectroscopic analysis, and the progress of reactions was monitored through Rf and thin-layer chromatography techniques. Melting points were recorded as part of the characterization process. In addition, an investigation into the biological activity of these compounds was conducted against both positive and negative bacterial strains.. KEYWORDS :Azo compounds, Oxazepine, Schiff bases.

Synthesis and Characterization of Heterocyclic Derivatives from 5-Aminotetrazole and Investigation of Their Biological Activities

This study included the synthesis of some new heterocyclic compounds starting with 5-aminotetrazole, which reacted with 2-hydroxy-4-methoxybenzaldehyde to get compound 1. Then, 1 was converted to a Schiff base (2) derivative by reacting 1 with 2-aminothiazole. Then, compound 2 was reacted with different compounds, namely alanine, 2-mercaptoacetic acid, 2-aminobenzoic acid, phthalic anhydride, and malic anhydride, leading to the heterocyclic products imidazolidine (3), thiazolidine (4), quinazoline (5), and oxazepane (6) derivatives, respectively. All these compounds are characterized by 13C-NMR, Fourier transform infrared spectroscopy, and 1HMNR. We also studied the biological activity of all heterocyclic derivatives toward two different kinds of bacteria and molecular docking studies.. KEYWORDS :Imidazolidine, Oxazepine, Quinazoline, Schiff bases, Thiazolidine.

Studies in synthesis of 6-aminocoumarin Schiff base derivatives as mesogens and their DFT approach

In first series, 6-amino coumarin Schiff base derivatives 10a-g were synthesized with alkoxy chain n = 6–18 and studied for their mesomorphic properties. In this series, compounds 10b with n = 8 showed monotropic nematic mesophase, while enantiotropic smectic A mesophase for higher analogues compounds 10c-g (with n = 10–18) was observed. In another series of Schiff base derivatives 12a-h additional ester linkage was introduced at the aldehyde end and were studied for their mesomorphic behavior. In second series, compounds 12a-c (with n = 4–8) showed enantiotropic nematic mesophase with very good thermal stability. Higher analogues of the second series with n = 12–18 showed stable enantiotropic smectic A mesophase. Compounds of both series were studied by DFT calculations. Different electronic parameters such as dipole moment, polarizability, molecular electrostatic potentials, frontier molecular orbitals, and thermodynamic variables were calculated and compared with the observed mesomorphic trends in both the studied series. Further, electronic parameters of compounds 10b and 12c and that of reference compound 4 were calculated and discussed to understand the effect of the removal of a hydroxyl group on the ortho position of the aldehyde part.

Ho(III)-coordination Complex: Fluorescence Performances and Combined with Captopril-Hydrogels against Glioma.

In this study, a novel dinuclear lanthanide complex, denoted as [Ho2(L)2(acac)2(CH3OH)2]·CH3OH (1) (where H2L is 2-[((4-methyl)-2-(carboxyl)-imino)]methyl]-8-hydroxyquinoline, and Hacac is acetylacetonate), was successfully synthesized and characterized using single-crystal and elemental analysis, with the auxiliary ligand β-diketonate and the 8-hydroxyquinoline Schiff base derivative serving as the foundation. Through ligand-to-metal charge transfer, Compound 1 demonstrated remarkable green fluorescence properties, showcasing potential applications in green fluorescence materials and fluorescence sensing. Additionally, Hyaluronic Acid (HA)/Carboxymethyl Chitosan (CMCS) hydrogels were synthesized through a chemical method. Utilizing captopril as a drug model, a novel metal gel granule of captopril was developed and assessed for its inhibitory activity against glioma cells. Molecular docking simulations revealed that only the methanol group in the Ho complex could contribute a polar atom for the formation of binding interactions.

Effectively regulation of the ESIPT process via ring-annelation modification for schiff base derivatives: A theoretical study

Precise regulation of the excited state intramolecular proton transfer (ESIPT) process using different new approaches remains a challenging task. Herein, based on an experimentally synthesized novel Schiff base (sp) which can be used as a Cr3+ ion sensor, the ESIPT mechanism and photophysical properties of six different Schiff base derivatives (b, 1n, 2n, 1a, 2a and sp) are theoretically analyzed by using the molecular ring-annelation modification to study the mechanism of their regulation for the ESIPT process. We find that the difference of the aromatic substituent at the hydrogen bond acceptor terminals will regularly enhance the excited-state intramolecular hydrogen bonds (IHBs) and weaken the intramolecular charge transfer (ICT) process. That is, the ring-annelation modification results in the expansive irradiative channels and restricted ICT process for Schiff base derivatives, thereby effectively reducing molecular ESIPT barrier. The above conclusions are confirmed by the qualitative and quantitative analyses of related hydrogen bonds and electron-hole. Our work provides a robust strategy for creating high-performance fluorescent chromophores via ESIPT manipulation.

Synthesis of Manganese(II), Iron(III), and Vanadium(IV) Complexes with New Schiff Bases and their Spectroscopic and Thermal Studies and Evaluation of their Antimicrobial Activity

Heterocyclic 3-acetyl coumarin with hydrazide derivatives and their metal complexes are a substantial family of pharmaceutical drugs used to treat infection, anti-inflammatory issues, diabetes, and neurological disorders in the field of medicinal chemistry. Cyclization of 5- floro-2-furaldehyde, ethyl acetoacetate, and urea or thiourea by LaCl3.7H2O, addition of hydrazine to form amine derivatives were performed, and respective Schiff base derivatives (L1, L2) were produced by adding acetyl coumarin in an ethanolic solution at ambient temperature. New ligands and its complexes of the V (IV), Fe (III) and Mn (II) ions were characterized using (FT-IR, UV, MS) and nuclear magnetic resonance (1H-NMR) as well as elemental analysis (CHN). The synthesized complexes chelate with ligands L1, L2 via (N, O2) atoms. The structural geometry of the complexes was illustrated in the solid phase using FT-IR and UV-VIS spectroscopy, elemental analysis (CHNS), and flame atomic absorption, in addition to magnetic susceptibility and conductivity measurement. The antibacterial activity of the newly prepared ligands and their metal complexes was evaluated against Pseudonomous aerugionosa as a gram negative and Bacillus subtilis as a gram positive microorganism. Moreover, the antifungal activity against two fungi Aspergillus flavus, and Saccharomyces cerevisiae was studied for all compounds. The coordinated ligands significantly increased their bactericidal and fungicidal action compared to the free ligands, which did not exhibit any activity against the selected fungal and bacterial strains. The results focused on the synergetic relationship between the metal ion and the ligand, in addition to the structural variation.