Using the techniques of seismic tomography three-dimensional velocity images at crust and upper mantle in Yunnan province and its adjacent region have been successfully reconstructed. The results of image are: (1) The image of the velocity in the upper crust is closely related to the well-known geological structure of the surface, the Kangdian earth axis is a distinct high velocity area, and a high velocity rock stratum, which appoaching the surface of the earth, has been formed. (2) There is a low-velocity layer between 26°–31°N and 100°–104°E in deep crust, the depth of Moho discontinuity in Sichuan bass in is less than 50 km. (3) The results of seismological tomography not only reveal the lateral heterogeneity in the researched region, but also find approximately the strike of Honghe fault from the image at bottom of crust, and the velocity in both side of the fault are different obviously. (4) There is a low-velocity column within 25 km to 110 km in Tengchong region, which may be occured by upward moving of the basalt in the mantle. (5) In studied area, the thickness of the crust in west part is thicker than in southeast part. (6) From the image at bottom of the crust we can find that earthquakes with magnitude greater than 5 occurred in big velocity gradient zones, especially in transition zone between high and low velocity. There are a few earthquake in the low-velocity area. (7) We can see from Figure 6 that there still clearly exists lateral heterogeneity at 450 depth.