Introduction: Depression is a leading source of disability world-wide withprevalence ranging from 8.8% to 18.3% in people aged 60 years or older. Depression is a not asimple term but has been used to describe a variety of infirmities, ranging from minor to debilitating.It is a multi-symptom disease and can even lead to suicidal attempt. Major depressive disorder(MDD), although one of the most common psychiatric illnesses, has an unknown etiology.Decreased serum levels of vitamin D have been implicated in the pathogenesis of MDD. Casecontrol studies have supported a relationship between low vitamin D as possible predictorsof depression. We designed a study to determine relationship, if any, between low vitaminD deficiency with depression and with severity of disease in our local population. Objective:The aim of this study to see any association of vitamin D deficiency in depressed Pakistanipatients. Study Design: Cross-sectional study. Setting: Fatima Memorial Hospital, Shadman,Lahore. Period: 2013-2015. Methods: Total of 150 diagnosed patients of 18-75 years of agewas enrolled for this study, after a written consent. Depressed patients were further categorizedinto three groups depending on severity of disease i.e.; mild, moderate and severely depressedpatients. Blood measures included serum vitamin D levels and data was recorded on structureddata collection form. Results: In this study, we investigated the association between vitamin Dstatus and depression in our local Pakistani population. Out of 150 depressed patients, 84.7%were vitamin D deficient. In our study, Out of 44 male depressed patients 9 (20.5%) had milddepression, 8 (18.2%) had moderate depression and 27 (61.4%) had severe depression. Outof 106 depressed female patients 42 (39.6%) had mild depression, 26 (24.5%) had moderatedepression and 38 (35.8%) had severe depression symptoms. A negative correlation of vitaminD with severity of disease was also found in female depressed patients in our study.A significantnegative correlation of vitamin D in all 44 male subjects with severity of disease was observedin our study. Higher prevalence of vitamin D deficiency was found in both males and females inour study. Conclusions: Vitamin D deficiency is associated with an increased risk of developingdepression, but more prospective observational studies may be needed. Efficacy of vitamin Dsupplementation for preventing onset of depressive disorder is unknown. Efficacy of vitamin Dsupplementation for reducing depressive symptoms is largely unstudied. The study suggestsdietary intervention with vitamin D would boost brain serotonin concentrations and help preventand possibly relieve some of the symptoms associated with depression without side effects.