PurposeThe purpose of this study is to examine the lasting effect of reproductive behaviour on career cycles for women. Women are the main bearers of population reproduction and family division of labour and a source of innovation for social and economic construction. However, few studies have used a comprehensive theoretical framework to research why female employees suffer from employment exclusion (EE) after multiple births. Therefore, structured equation modelling is used to test the degree of fit between the theoretical model and the data, to verify whether the hypotheses are valid and then make an appropriate interpretation of the theoretical model.Design/methodology/approachThe paper focusses on working mothers with two or more children and is based on questionnaire surveys, with 306 pieces of primary data obtained. This paper constructed the chained mediation model on the theoretical basis of the conservation of resources theory to examine and analyse the mechanisms of family–work conflict (FWC) and human capital depreciation (HCD) on EE.FindingsEmpirical results suggested that FWC has a significant positive impact on EE, while HCD does not. Chain mediation variables of family orientation (FO) and work engagement (WE) mediated the relationship between FWC and EE significantly, as well as between HCD and EE. Each antecedent positively predicted FO and, through FO, indirectly affected WE, then indirectly affected EE.Research limitations/implicationsIn the context of fertility policy adjustment and population structural imbalance, research from the perspective of females may better reflect reality and deepen understanding of EE to avoid it.Originality/valueFrist, relevant studies mostly study the factors affect in women’s equal employment from the macro levels, and focus on qualitative research, and lack sufficient empirical analysis. Second, this study locates the research object in the group of working mothers with two or more children. The focus of the research objects makes the research conclusions help to improve the work dilemma of special groups. Third, this study has opened up the “black box” between employees’ individual resources and work attitudes and behavior tendencies. Fourth, this study expands the possible outcome variables of FWC.