Deposition Area Research Articles

Three-Dimensional Rockslide Analysis Using Unmanned Aerial Vehicle and LiDAR: The Castrocucco Case Study, Southern Italy

Rockslides are one of the most dangerous hazards in mountainous and hilly areas. In this study, a rockslide that occurred on 30 November 2022 in Castrocucco, a district located in the Italian municipality of Maratea (Potenza province) in the Basilicata region, was investigated by using pre- and post-event high-resolution 3D models. The event caused a great social alarm as some infrastructures were affected. The main road to the tourist hub of Maratea was, in fact, destroyed and made inaccessible. Rock debris also affected a beach club and important boat storage for sea excursions to Maratea. This event was investigated by using multiscale and multisensor close-range remote sensing (LiDAR and SfM) to determine rockslide characteristics. The novelty of this work lies in how these data, although not originally acquired for rockslide analysis, have been integrated and utilized in an emergency at an almost inaccessible site. The event was analyzed both through classical geomorphological analysis and through a quantitative comparison of multi-temporal DEMs (DoD) in order to assess (i) all the morphological features involved, (ii) detached volume (approximately 8000 m3), and (iii) the process of redistributing and reworking the landslide deposit in the depositional area.

Development and characterization of a chronic high intraocular pressure model in New Zealand white rabbits for glaucoma research

Glaucoma is a neurodegenerative disease characterized by visual field loss associated with optic nerve damage and ocular hypertension. The biological basis for the elevated intraocular pressure (IOP) is largely unknown, such that lowering the IOP is currently the only established treatment. Several animal models have been developed to elucidate the mechanism underlying the increased IOP and for use in drug discovery research, but their utility is often limited by the occurrence of severe intraocular inflammation and by technical challenges. In this study, we developed a rabbit glaucoma model that does not require experimental disease induction. Rabbits were chosen as the model because their eyeballs are similar in size to those of humans, and they are easy to breed.By crossing rabbit strains with inherited glaucoma, as indicated by obvious buphthalmos, we produced a strain that exhibits ocular hypertension. The IOP of the Ocular Hypertension (OH) rabbits was significantly higher than that of the wild type (WT; normal New Zealand white rabbits) from the age of 3 weeks to at least 22 weeks. The significantly larger corneal diameter of the OH rabbits indicated ocular enlargement, whereas there was no significant difference in corneal thickness compared with WT rabbits. Anterior segment ocular coherence tomography and gonioscopic observations revealed an open angle in the OH rabbits. Hematoxylin and eosin (HE) staining together with Masson's trichrome staining showed abnormal collagen accumulation in the angle of the OH rabbit's eyes. Furthermore, aqueous humor (AH) outflow imaging following an intravitreal injection of a fluorescent probe into the anterior chamber for tissue-section analysis revealed retention of the probe in the area of collagen deposition in the OH eyes. The OH rabbits also had a time-dependent increase in the cup/disc ratio.In conclusion, investigations using our newly developed rabbit model of open-angle ocular hypertension showed that abnormal accumulation of extracellular matrix at the angle increased AH outflow resistance in the conventional outflow pathway, leading to a high IOP. Furthermore, the OH rabbits exhibited glaucomatous optic disc cupping over time. These findings suggest the utility of the OH rabbits as a model for open-angle glaucoma (OAG).

Legacy effects of historical gold mining on floodplains of an Australian river

The gold rush at the end of the nineteenth century in south-eastern Australia resulted in the mobilization and re-deposition of vast quantities of tailings that modified the geomorphology of the associated river valleys. Previous studies of contamination risk in these systems have either been performed directly on mine wastes (e.g., battery sand) or at locations close to historical mine sites but have largely ignored the extensive area of riverine alluvial deposits extending downstream from gold mining locations. Here we studied the distribution of contaminant metal(loids) in the Loddon River catchment, one of the most intensively mined areas of the historical gold-rush period in Australia (1851–1914). Floodplain alluvium along the Loddon River was sampled to capture differences in metal and metalloid concentrations between the anthropogenic floodplain deposits and the underlying original floodplain. Elevated levels of arsenic up to 300 mg-As/kg were identified within the anthropogenic alluvial sediment, well above sediment guidelines (ISQG-high trigger value of 70 ppm) and substantially higher than in the pre-mining alluvium. Maximum arsenic concentrations were found at depth within the anthropogenic alluvium (plume-like), close to the contact with the original floodplain. The results obtained here indicate that arsenic may pose a significantly higher risk within this river catchment than previously assessed through analysis of surface floodplain soils. The risks of this submerged arsenic plume will require further investigation of its chemical form (speciation) to determine its mobility and potential bioavailability. Our work shows the long-lasting impact of historical gold mining on riverine landscapes.

Statement of Retraction: Discovery and significance of ancient cod fossils in hydrothermal fluid deposition areas: a case study of Chang 7-3 from the Triassic Yanchang Formation in the Ordos Basin

Statement of Retraction: Discovery and significance of ancient cod fossils in hydrothermal fluid deposition areas: a case study of Chang 7-3 from the Triassic Yanchang Formation in the Ordos Basin

Unraveling radiation resistance strategies in two bacterial strains from the high background radiation area of Chavara-Neendakara: A comprehensive whole genome analysis.

This paper reports the results of gamma irradiation experiments and whole genome sequencing (WGS) performed on vegetative cells of two radiation resistant bacterial strains, Metabacillus halosaccharovorans (VITHBRA001) and Bacillus paralicheniformis (VITHBRA024) (D10 values 2.32 kGy and 1.42 kGy, respectively), inhabiting the top-ranking high background radiation area (HBRA) of Chavara-Neendakara placer deposit (Kerala, India). The present investigation has been carried out in the context that information on strategies of bacteria having mid-range resistance for gamma radiation is inadequate. WGS, annotation, COG and KEGG analyses and manual curation of genes helped us address the possible pathways involved in the major domains of radiation resistance, involving recombination repair, base excision repair, nucleotide excision repair and mismatch repair, and the antioxidant genes, which the candidate could activate to survive under ionizing radiation. Additionally, with the help of these data, we could compare the candidate strains with that of the extremely radiation resistant model bacterium Deinococccus radiodurans, so as to find the commonalities existing in their strategies of resistance on the one hand, and also the rationale behind the difference in D10, on the other. Genomic analysis of VITHBRA001 and VITHBRA024 has further helped us ascertain the difference in capability of radiation resistance between the two strains. Significantly, the genes such as uvsE (NER), frnE (protein protection), ppk1 and ppx (non-enzymatic metabolite production) and those for carotenoid biosynthesis, are endogenous to VITHBRA001, but absent in VITHBRA024, which could explain the former's better radiation resistance. Further, this is the first-time study performed on any bacterial population inhabiting an HBRA. This study also brings forward the two species whose radiation resistance has not been reported thus far, and add to the knowledge on radiation resistant capabilities of the phylum Firmicutes which are abundantly observed in extreme environment.

High-resolution water temperature and salinity evolutions and associated drivers of the North Yellow Sea during the Late-Holocene

The mud deposit areas on continental shelf have developed in a relatively steady sedimentary condition with high sedimentation rates, thus rendering them ideal regions for exploring high-resolution paleo-sedimentary environment records. Since the sedimentary environment of the continental shelf is subject to the compound influence of multiple factors, the reconstruction of water salinity and temperature and comprehensive analysis of their response to global climate change remains challenging. Therefore, the present study characterized water salinity and temperature of the North Yellow Sea (NYS) using proxies, such as ratios of halogen elements of sediments and stable isotopic compositions of benthic foraminifera, and found the halogen elements of Cl in sediments was good proxy for water salinity. The evolutions of water salinity and temperature of the NYS for the past 3000 years were reconstructed, while the evolution stages and drivers of the sedimentary environment were explored. From approximately 3000 cal yr BP, the sea bottom salinity and temperature of the NYS were found to follow the same evolution trends predominantly consisting of the three stages: relatively low seawater salinity and temperature during 3000−2000 cal yr BP; relatively high seawater salinity and temperature during 2000−460 cal yr BP; and rapid changes of seawater salinity and temperature since 460 cal yr BP. The changes in seawater salinity and temperature of the NYS were largely driven by the East Asian winter monsoon (EAWM) as well as the influence of the Kuroshio Current. As the EAWM fluctuated, changes occurred in the flux of low temperature, low salinity coastal current water into the Yellow Sea, with concomitant changes in bottom temperature and salinity. Strengthening of the Kuroshio Current promoted the development of the Yellow Sea Warm Current, while the influx of warm, salty water into the Yellow Sea influenced the respective sea region. The abrupt global climate changes such as the “Little Ice Age” and the Medieval Warm Period first affected the East Asian monsoon, followed by the respective sea region.

Enhancing microscale additive manufacturing: Electrolyte-column localized electrochemical deposition for microwire and microdevice substrate connection

Electrochemical deposition is a microscale additive manufacturing technology. Due to the excellent performance of electrodeposited coating layers which exhibit no thermal stress, low porosity, and minimal microcracks, electrodeposition has been used in the connections of microdevices to solve the problems of shrinkage and deformation of microparts caused by thermal stress generated by conventional connections. Due to the low efficiency of Meniscus-Confined electrodeposition, a novel connection process for microwires and microdevice substrate using electrolyte-column localized electrochemical deposition (ECL-ECD) is proposed. The connecting mechanism is determined through simulation and experimentation. Simulation results indicated that the current density in the connection area exhibited a Gaussian distribution and the deposition layer preferentially grew at the interface between the microwire and the substrate. A high-speed camera was used to observe the liquid beam throughout the experiment, the results indicated that the microwires didn’t affect the stability of the liquid beam, and the ECL-ECD had excellent domain fixation. Smooth and dense surfaces on the connection area of the microwire and substrate were obtained at a current density of 50–60 A/dm2. Performance testing results showed that the connection point has excellent corrosion resistance and electrical properties. When the deposition time was short, the microwire was detached from the deposition area due to relatively thin deposition layer thickness and the tensile strength was only 133.82 MPa during the tensile testing. When the appropriate deposition process parameters were chosen, the fracture occurred on the microwire under tensile load and the fracture strength was 198.84 MPa, which equals the tensile strength of the microwire and indicated that the connection point can provide sufficient strength. The experimental results demonstrated that ECL-ECD can provide an effective and reliable connection process for the microwires interconnection.

Dynamic changes in autophagy activity in different degrees of pulmonary fibrosis in mice.

The aim of this study is to observe the changes in autophagy activities in lung tissues of mice with different degrees of pulmonary fibrosis (PF), and explore the association between PF and autophagy activity. The PF model was established by bleomycin (BLM, 25 and 35 mg/kg) atomization inhalation in C57BL/6 mice, samples were collected on the 7, 14, and 28 days after BLM administration. Hematoxylin-eosin staining was used to observe the pathological changes in lung tissues. Masson staining was utilized to assess areas of blue collagen fiber deposition in lung tissues. Quantitative real time polymerase chain reaction was used to detect the mRNA expressions of autophagy-related genes, including Atg5, Atg7, and Atg10 in lung tissues. Western blot was used to detect the protein expressions of autophagy-related genes, including p62 and LC3II/LC3I in lung tissues. Compared with control group, BLM dose-dependently decreased PaO2, mRNA expressions of Atg5, Atg7, Atg10, and LC3II/LC3I, while increased lung wet weight, lung coefficient, PF score, the blue area of collagen fibers, and p62 protein on the 7th, 14th, and 28th days. In conclusion, the more severe the PF induced by BLM, the lower the autophagy activity.

Hydrologic evolution of two Martian deltas in the Ismenius Cavus system

Ismenius Cavus (33.76° N, 17.05° W) is a large Martian basin that has been interpreted as an ancient paleolake. It is situated in the middle of the path Mamers Valles traces from the Cerulli Crater to the Borealis Basin. This ancient lake served as a key deposition area (or depocenter) for multiple lake chains originating in the southern highlands. The collected water was then transported downstream to the Borealis Basin. The ancient hydrologic activity of this zone is evidenced in the widespread appearance of valley networks and fretted channels, but especially in the presence of deltaic deposits converging in Ismenius Cavus. We made a hydrological and geomorphological analysis of two of these deltas, which compose a three-crater lake chain system. It was interpreted that both deltas, although being close to each other, were created by different processes and at different times. The Aracataca Delta was deposited during the Noachian by a valley network system. The Ariguani Delta, in turn, was the result of the discharge of a fretted channel carved by groundwater sapping. The transformations of the hydrologic systems in the Ismenius Cavus region were established by analyzing the change in the deltaic pulses over time. It was determined that the first hydrologic stage was dominated by widespread valley networks that probably represent subglacial hydrologic systems, a second stage started when these systems were replaced by groundwater activity; and finally, when the water sources were exhausted, a final stage of glacial processes prevailed until the present.

Smoothed particle hydrodynamics simulation of debris flow on deposition area

Smoothed particle hydrodynamics simulation of debris flow on deposition area

Origin of ferruginous coated grains in the Lower Jurassic palustrine limestones of the Pranhita–Godavari Basin, India

AbstractThe formation of calcareous coated grains like ooids and oncoids have been extensively researched. In contrast, the origin and genesis of ferruginous coated grains are less clear. Radically different processes like surficial weathering, hydrothermal exhalation and microbial activity have been advocated for the source of iron and the development of coatings. Modern examples are sparse and from marine environments affected by igneous activity. This study presents the features of ferruginous coated grains found in freshwater lacustrine–palustrine deposits of the Lower Jurassic syn‐rift strata of the Pranhita–Godavari Basin, India. The coated grains occur in metre‐thick lenticular bodies of nodular limestones enclosed in black mudstones containing plant debris and sulphates (gypsum and baryte). In the nodular limestones, sand‐size coated grains, along with chert and quartz grains, float in a micritic groundmass. Angular quartz grains embedded in dark, amorphous haematite–goethite constitute the nuclei of the coated grains. Cortices comprise numerous, thin, wavy laminae of goethite with trapped detrital grains. These coated grains are typically devoid of carbonates, although the outermost part of the cortex is replaced by calcite spars to varying extent. The black mudstone‐nodular limestone association occurs in between siliciclastic lacustrine deposits (laminated red and green mudstones) below, and the palustrine limestones‐green shale association above. The palustrine limestones contain remains of aquatic organisms, evidence of desiccation, paedogenesis, microbial‐mat formation, chert, baryte and gypsum layers. A few decimetre‐scale, wedge‐shaped, flood‐derived cross‐bedded sandstones occur locally. Due to the proximity of the depositional area to the marginal fault system of the rift basin and association of the limestones containing coated grains with sub‐aerially weathered microbialites, these ferruginous coated grains are ideally poised for a comparative assessment of various hypotheses of solute supply and precipitation. Based on field, microscopic and chemical characteristics, it is possible to conclude that microbially mediated precipitation in hydrothermal (hot spring)‐influenced pools is the most probable origin for these ferruginous coated grains.

Endoscopic diagnosis and treatment of lichen planus of the esophagus

Purpose of the study: to attract the attention of endoscopists and doctors of other specialties to the problem of diagnosis and treatment of lichen planus of the esophagus. Materials and methods. From January 2010 to December 2023, lichen planus was the cause of dysphagia in 7 of 17 patients with unexplained cicatricial strictures of the esophagus. Our own experience and literature data on endoscopic semiotics and treatment of lichen planus of the esophagus are presented. Results of the study. Lichen planus is most often localized in the upper third of the esophagus and is accompanied by a narrowing of the esophageal lumen. The mucosa is hyperemic, dull, with areas of epithelial detachment, erosions, and fibrin deposits. All 7 patients had grade 2-3 esophageal strictures with isolated esophageal involvement (3) or oral involvement (4). Morphological changes were nonspecific: ulcerations, granulation tissue and fibrosis of the underlying layers, atrophy and thinning of the epidermis, acanthosis. Only in 2 cases apoptotic Civatte bodies were detected in the suprabasal layer, which is a characteristic feature of lichen planus of the esophagus. All patients underwent courses of endoscopic bougienage, supplemented by intramural injections of triamcinolone. This manipulations led to stabilization of the esophageal lumen at 10-15 mm without a tendency to restenosis. Conclusion. Further experience is needed to determine optimal treatment strategies, but it is critical to pay particular attention to symptom assessment in patients with skin disorders and odynophagia or dysphagia. These actions will facilitate an earlier diagnosis of lichen planus of the esophagus and increase the effectiveness of endoscopic treatment.

Dynamic analysis of field-scale rockslides based on three-dimensional discontinuous smoothed particle hydrodynamics: A case study of Tangjiashan rockslide

To improve the understanding of the dynamic disastrous process of field-scale rockslides, a novel numerical approach, Three-dimensional Discontinuous Smoothed Particle Hydrodynamics (3DSPH), was originally developed. This method comprehensively captures sequential stages of crack initiation and propagation, formation of contacts, frictional slip, catastrophic slides of rock masses, and final deposition, which was verified by three benchmark tests including the bouncing test of rigid balls, block sliding test and unconfined compression test of layered rock specimens. The approach was subsequently employed in the case of the Tangjiashan rockslide blocking valley event with particular attention paid to the influence of layered rock structure on the sliding and deposition processes. Field survey, geomorphological analysis and laboratory test of rock specimens were conducted to determine fundamental geological conditions and parameters required by the numerical simulation. Finally, 3D rockslide simulations with different rock layer thickness and strength subject to seismicity were conducted. The duration of the actual Tangjiashan rockslide's valley-blocking event (approximately 60 s) and the deposition area derived from the numerical simulation closely align with the field investigation. The rockslide mode is characterized by an ‘en masse’ motion with a peak sliding velocity of approximately 35–37 m/s. This single numerical code systematically elucidated the authentic attributes of the sliding process of large-scale rockslides, and realistically captured the characteristics of preservation of layered features within the sliding mass and ‘high-speed and short-distance’ movements with fluidization. These insights offer a fresh perspective for understanding the dynamics of large-scale rockslides with complex geological structures and subsequent accumulation processes.

Erosive hand osteoarthritis and sarcopenia: data from Osteoarthritis Initiative cohort

ObjectivesThere is no evidence linking specific osteoarthritis (OA) types, such as erosive hand OA (EHOA), with distant generalised changes in muscle composition (sarcopenia), which can potentially be modified. This study...

Residual stress variations in substrate (AISI D2) during directed energy deposition process of high-speed tool steel (AISI M4) powder

This study aims to observe the residual stress in a substrate and predict stress behavior during the laser deposition process (DED) using finite element analysis (FEA). The residual stress observed on the substrate surface indicated that stress variation during the deposition process increases with proximity to the deposition area, resulting in higher residual stress levels. Additionally, tensile residual stress tends to increase with the height of the deposition area. While variations in the deposition area size influenced the residual stress, consistent stress levels were observed at the same measurement points across different area sizes. The deposition process was simulated using FEA, which confirmed that stress behavior is influenced by melting and solidification cycles. The residual stress levels after cooling aligned well with those observed in actual experiments. Therefore, this study suggests that stress variations can be effectively predicted by simulating the deposition process prior to conducting actual experiments.

Exploration Vectors and Indicators Extracted by Factor Analysis and Association Rule Algorithms at the Lintan Carlin-Type Gold Deposit, Youjiang Basin, China

The Youjiang Basin in China is the world’s second-largest concentrated area of Carlin-type Au deposits after Nevada, USA, boasting cumulative Au reserves nearing 1000 t. This study examined the recently unearthed Lintan Carlin-type Au deposit within the Youjiang Basin. Factor analysis and association rule algorithms were used to identify exploration vectors and indicators essential for navigating this promising geological territory. In the Lintan mining area, the geological strata encompass the Triassic Bianyang, Niluo, and Xuman formations comprised clastic rocks, followed by the deeper Permian Wujiaping Formation with massive carbonate rocks. The orebodies are restricted to the F14 inverse fault, cutting through the Xuman Formation, with an additional F7 fault between the Wujiaping and Xuman formations. A total of 125 rock samples from the F14 fault and a representative cross-section were analyzed for 15 elements (Au, Ag, As, Bi, Cd, Co, Cu, Hg, Mo, Ni, Pb, Sb, Tl, W, and Zn). The elements were divided into four groups based on cluster and factor analysis. Group 1 (Co, Cu, Zn, Ni, Tl, W, and Bi) was mainly enriched in the Xuman, Niluo, and Bianyang formations controlled by sedimentary diagenesis. Group 2 (Au, As, Hg, and Sb) was concentrated in the F14 and F7 faults, representing Au mineralization. Group 3 (Pb, Ag, and Mo) was mostly enriched near the F14 and F7 faults, displaying a peripheral halo of Au mineralization, and was probability controlled by ore-forming hydrothermal activities. Group 4 (Cd and Mo) exhibited extreme enrichment along the periphery of the F7 fault. This pattern indicates the presence of a substantial hydrothermal alteration zone surrounding the fault, likely influenced by ore-forming hydrothermal processes. Additionally, Pb, Ag, Cd, Mo, and W are considered essential indicators for ore formation besides Au, As, Sb, Hg, and Tl. Twelve effective association rules were derived using the association rule algorithm, which can aid in discriminating Au mineralization. The spatial distributions of the 15 elements indicated that the F14 fault is the main ore-bearing fracture zone, while the F7 fault serves as the ore-conducting structure, channeling ore-forming fluids into the F14 fault. Faults between the Wujiaping and Xuman formations, along with their associated reverse faults, present potential prospecting targets both within and outside the Lintan Au deposit in the Youjiang Basin. Exploration geochemical data can be fully utilized by combining factor analysis and association rule algorithms, offering key guidance for prospecting Carlin-type gold and similar deposits.

High-resolution soil erosion mapping in croplands via Sentinel-2 bare soil imaging and a two-step classification approach

Erosion-induced lateral soil redistribution leads to spatially heterogenous soil composition, which can be captured through the distinctive spectral reflectance of soils under varying levels of erosion influence. This points to the potential of using remotely sensed soil spectra to detect severe erosion and deposition hotspots in exposed croplands and, importantly, further differentiate the intra-class spectral variability of moderate erosion that often occupies the largest proportion. Here, we aim to develop a two-step erosion classification and mapping approach based on multitemporal compositing of Sentinel-2 bare soil images of a typical agricultural region (11,500 km2) of northeast China. A random forest classifier was firstly trained against the ground-truth data derived from very high resolution (VHR) imagery in Google Earth, with an overall accuracy of 91 % that allowed for clear delineation of severe erosion and deposition areas based on their distinct topographic and spectral features particularly in the red and red-edge bands. In the second step, the remaining area of moderate erosion (60.30 %) was further differentiated using Iterative Self-Organizing cluster unsupervised classification to yield a five-class soil erosion map at 10 m spatial resolution. The accuracy of the predicted map was successfully validated by independent Caesium-137 (137Cs) and soil organic carbon observations at catchment and regional scales, as revealed by significant inter-class differences in soil redistribution rates estimated from 137Cs inventory. The severe erosion class had a soil loss rate of 5.5 mm yr−1, suggesting that previous assessments have underestimated erosion severity. The spatial accordance of crop growth with soil erosion intensity, particularly in localized settings, further highlighted the potential of bare soil imaging for mapping the spatiotemporal development of soil erosion and its response to targeted sustainable cropland management efforts.

The ablation behavior and modification mechanism of SiC under different laser energy

The laser modification pretreatment method is an effective way to address the machining difficulties of silicon carbide (SiC), a typical hard-to-machine material. Therefore, the ablation behavior and modification mechanism of SiC under different laser energy were explored in this paper. A new multi-scale model of laser irradiation SiC that couples heat transfer and fluid motion is first established. Then, a series of experiments is carried out to evaluate the model's accuracy, and the interaction between laser and SiC is discussed in detail. The results show that the dominant modification mechanism changes from coulomb explosion to multiphoton absorption, incubation effect, and heat accumulation with the laser energy increase. This leads the surface topography of SiC to transition from nanoparticle formation to disorder to a melting state. In ablative state, micro/nano porous and humps are formed at the edge of ablation groove due to surface tension, generation and rupture of bubbles, respectively. Furthermore, the surface roughness is not proportional to the laser energy due to the plasma shielding effect, and the surface roughness can be reduced by enhancing the flow of the molten material. Amorphous Si–O–C, Si and spherical SiO2 exist in deposition area, leading to SiC elastic modulus decreases from 347 GPa to 103.82 GPa, and the shear strength decreases from 20.9 GPa to 17.25 GPa. The results of this study can provide references for parameters selection and theoretical support for improving the machinability of SiC.

Arthroscopic Decompression of Calcific Tendinitis of the Shoulder and Repair of Residual Rotator Cuff Defect

Background: Calcific tendinitis is a common source of shoulder pain and represents pathologic deposition of calcium hydroxyapatite within rotator cuff tendon tissue, most commonly the supraspinatus tendon. Indications: Arthroscopic decompression of calcific tendinitis with possible rotator cuff repair is indicated in patients with persistent, debilitating symptoms of pain and/or dysfunction who are recalcitrant to nonoperative treatments, including corticosteroid administration, ultrasound-guided needle barbotage, and/or extracorporeal shockwave therapy. Technique Description: With the patient in a beach chair position, a standard diagnostic shoulder arthroscopy is performed to evaluate for concomitant pathologies. Within the subacromial space, a thorough bursectomy is performed and the area of calcium deposition is localized with a spinal needle. A scalpel may be used to create a small incision through the rotator cuff tendon in line with its fibers to promote egress of calcific debris. Surrounding tissue and loose debris are removed with an arthroscopic shaver. Following decompression, the rotator cuff repair is inspected, and if a bursal-sided or full-thickness tear is identified, an arthroscopic repair is performed with a construct individualized to the specific tear pattern. Results: Surgical treatment conferred greater functional improvement and comparable pain reduction to nonoperative treatments in a systematic review comprised of 27 randomized trials. While the addition of a rotator cuff repair remains controversial, combined excision of calcific tendinitis with concomitant rotator cuff repair led to greater functional outcomes and pain reduction at 2-year minimum follow-up compared with isolated decompression. Conclusion: Calcific tendinitis within the shoulder may be treated successfully with arthroscopic decompression and subsequent repair of a residual rotator cuff defect, followed by a graduated physical rehabilitation program. Patient Consent Disclosure Statement: The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication.

Structure-Function Relationship of Respiratory Muscles in the mdx Mouse Model of Duchenne Muscular Dystrophy

Duchenne muscular dystrophy (DMD) is a genetic neuromuscular disorder, characterized by severe and progressive skeletal muscle weakness, which extends to the respiratory muscles. We have determined that peak inspiratory pressure-generating capacity is preserved in early dystrophic disease in the mdx mouse model of muscular dystrophy, but this early compensation is lost as disease progressesa. We sought to examine the structure-function relationship of obligatory and accessory respiratory muscles in early (4-month) and advanced (16-month) disease. Ex vivo muscle function of obligatory (diaphragm and parasternal) and accessory (sternomastoid, cleidomastoid and scalene) respiratory muscles was assessed in wild-type and mdx mice in early dystrophic disease (n = 8-15 per group). Diaphragm and scalene ex vivo muscle function was assessed in advanced dystrophic disease (n = 10-14 per group). Histology (sirius red, and haematoxylin and eosin) and immunofluorescence (laminin) were used to assess the form of obligatory (diaphragm, parasternal and intercostal) and accessory (sternomastoid, cleidomastoid, scalene and trapezius) respiratory muscles (n=6 per group). Pro-inflammatory cytokine concentrations were examined in muscle homogenates of obligatory and accessory respiratory muscles of wild-type and mdx mice in early and advanced dystrophic disease (n=10 per group). Our study revealed obligatory (diaphragm and parasternal) and accessory (sternomastoid and cleidomastoid) muscle weakness evident in early dystrophic disease. Scalene muscle function is preserved in mdx mice in early disease but is weak in mdx mice in advanced dystrophic disease. Structural remodelling by way of increased collagen deposition (fibrosis), increased central nucleation, increased area of inflammatory cell infiltrate and reduced mean minimal Feret’s diameter (relative atrophy) was evident in obligatory and accessory respiratory muscles in early dystrophic disease. The relative area of collagen deposition in obligatory and accessory respiratory muscles increased with disease progression, but interestingly the increase was most pronounced in the diaphragm muscle. Increased concentration of pro-inflammatory cytokines (IFN-γ, IL-1β, IL-6, KC/GRO, TNF-α, MCP-1, IL-33, MIP-1α, IP-10, MIP-2) was observed in mdx obligatory and accessory muscle homogenates compared to wild-type in early and advanced dystrophic disease. These data widen characterisation of the respiratory control system in mdx mice, potentially revealing novel therapeutic targets. References a O'Halloran KD, Maxwell MN, Marullo AL, Hamilton CP, Ó Murchú SC, Burns DP, Mahony CM, Slyne AD, Drummond SE (2023). Loss of compensation afforded by accessory muscles of breathing leads to respiratory system compromise in the mdx mouse model of Duchenne muscular dystrophy. J Physiol. 601(19):4441-4467. Eli Lilly & Science Foundation Ireland (19/FFP/6628). This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.