Mass Depletion Research Articles

Automated Assessment of Sarcopenia with Hounsfield Unit Average Calculation in Computed Tomography Scans Using Deep Learning Techniques

Abstract Introduction Skeletal muscle is increasingly plastic with an ability to gain or lose tissue. Depletion of muscle mass and quality occurs due to various factors such as aging, disease, and disuse. Sarcopenia can be loosely defined as a significant loss of muscle mass and function. Sarcopenia is now recognized as an independent risk factor for various patient-related negative outcomes after various surgeries. Various computed tomography (CT) based imaging indices for assessment of sarcopenia exist in practice. The psoas muscle Hounsfield unit average calculation (HUAC) has been proven to be an effective one as it is independent of patient anthropometric data, and it can be calculated in the images provided. Aim The aim of this study is to develop automated tools for estimation of the HUAC using deep learning algorithms. Materials and Methods A total of 41 abdominal CTs were used. Ground truth was established and validated by two radiologists with more than 5 and 10 years of experience each. Models were trained to identify the psoas muscle among the slices and calculate the HUAC. Results At inference, an average intersection over union (IoU) value of 90% was obtained between the deep learning model outputs and the original annotated test images for the CT slices. The Dice coefficient was 0.90 between the ground truth labels and the output from the model. Conclusion We have demonstrated the accuracy of our deep learning–based algorithm for quantifying the psoas muscle HUAC, which is a marker for sarcopenia. There is a potential for a fully automated measure to calculate the HUAC for any patient undergoing CT scan.

Increasing density and viscosity of light non-aqueous phase liquids are indicators of natural source zone depletion

Natural source zone depletion (NSZD) acts to reduce the mass and/or toxicity of light non-aqueous phase liquids (LNAPL) in the shallow subsurface. Several indirect measurement techniques are commonly used to estimate LNAPL mass depletion based on CO 2 generation by hydrocarbon mineralization, heat production by exothermic biodegradation processes, and subsurface gas concentration gradients. This paper presents data on LNAPL density and viscosity and shows changes that result from NSZD processes. Increasing trends in LNAPL density and viscosity were observed that provided a qualitative line-of-evidence for NSZD. When LNAPL density and viscosity were combined with contemporaneous LNAPL chemistry analysis it was possible to correlate changes in the physical properties to depletion of alkanes from the LNAPL in a manner that would inform a quantitative NSZD assessment. LNAPL density and viscosity measurements are relatively cheap and reproducible, and increasing trends in LNAPL density and viscosity provide additional lines-of-evidence for NSZD.

Gender-Specific Malnutrition and Muscle Depletion in Gastric and Colorectal Cancer: Role of Dietary Intake in a Jordanian Cohort.

This study aimed to assess malnutrition and muscle mass depletion risk in gastrointestinal cancer patients, exploring the differences between gastric and colorectal cancer, with a focus on gender0specific variations and dietary intake. It also examined whether muscle depletion mediates the relationship between dietary intake and malnutrition risk. A sample of 100 Jordanian pre-operative gastrointestinal cancer patients (60 male, 40 female) with gastric or colorectal cancer were assessed for malnutrition risk using the malnutrition universal screening tool (MUST) and for muscle depletion using fat-free mass index (FFMI) and mid-upper arm muscle area (MUAMA). The study found that 80% (95% CI: 0.708-0.873) of patients were at high risk of malnutrition, with over 60% experiencing severe muscle loss. Gastric cancer patients showed higher, though not statistically significant, malnutrition risk (90.2% vs. 72.9%) and muscle depletion compared to colorectal cancer patients. Advanced cancer stages were associated with significantly higher risk of malnutrition and muscle depletion. Significant gender-specific differences in muscle depletion via FFMI (p = 0.012) and via MUAMA (p = 0.028) were also noted, especially in females with gastric cancer. Additionally, males exhibited a significantly higher malnutrition risk (p < 0.001) based on cancer stage. Patients' dietary intake was significantly (p < 0.001) below the recommended levels for energy, protein, carbohydrates, fiber, and essential fatty acids, which was associated with higher malnutrition risk, muscle depletion, low BMI (<18.5 kg/m2), and significant weight loss (>10%). Low dietary intake was strongly linked to increased malnutrition risk and muscle depletion, with muscle loss partially mediating (b = 0.4972, p < 0.0001) the relationship between poor dietary intake and malnutrition risk. Additionally, higher muscle mass was protective against malnutrition (OR = 16.0, 95% CI: 1.706-150.507), and cancer type was a significant predictor of malnutrition risk (OR = 14.4, 95% CI: 1.583-130.867). Malnutrition risk and significant muscle loss are common in GI cancer patients, highlighting the urgent need for tailored nutrition care plans and lifestyle modifications.

Influence of Gas Washing and Oil Mixing on the Phase Behavior and Physical Properties of Cambrian Light Oils in the Tazhong Uplift, Tarim Basin

Gas washing and oil mixing have been widely studied in the Tazhong area of the Tarim Basin, but their effects on Cambrian subsaltic dolomite reservoirs have not yet been adequately explored. In this study, the influence of gas washing and oil mixing on light oils from these reservoirs was investigated. Using n-alkane mass depletion (Q value) analysis, light oils from wells ZS1 and ZS5 were analyzed to quantify the intensity of gas washing and to identify possible oil mixing in the Cambrian Awataga (Є2a) and Wusongar (Є1w) reservoirs. Unaltered light oil was recovered and models of phase behavior were constructed by PVT simulation and basin modeling to compare with altered light oils. The results show that the light oil in well ZS1 experienced medium-strength gas washing (Q value = 40.88%), while the gas washing in well ZS5 was weaker (Q value &lt; 40.88%), providing evidence of oil mixing. Despite these changes, the light oils in the Cambrian reservoirs maintained a single liquid-phase state over geologic time without transitioning to condensate or gas phases. These results improve the understanding of gas washing in the Tazhong area and show that it has significantly altered the Cambrian reservoirs and affected the preservation of hydrocarbons after oil accumulation.

New Results on the Onset of a Coronal Mass Ejection from 5303 Å Emission Line Observations with VELC/ADITYA-L1

We report on the onset of a coronal mass ejection (CME) using spectroscopic observations in the 5303 Å coronal emission line with the Visible Emission Line Coronagraph (VELC) onboard ADITYA-L1, the recently launched first Indian space solar mission. The CME was observed on 2024 July 16 in association with an X1.9 class soft X-ray flare from heliographic location S05W85. The VELC observations were near the west limb of the Sun during the CME. The results obtained helped to constrain the onset time of the CME. In addition, they indicate a ≈50% decrease in the coronal intensity near the source region of the CME due to mass depletion, a ≈15% enhancement in the emission line width, and a redshifted Doppler velocity of about ≈10 km s−1. The nonthermal velocity associated with the line broadening is ≈24.87 km s−1.

Targeting MuRF1 to Combat Skeletal Muscle Wasting in Cardiac Cachexia: Mechanisms and Therapeutic Prospects.

Cardiac cachexia, the terminal stage of chronic heart failure, is characterized by severe systemic metabolic imbalances and significant weight loss, primarily resulting from skeletal muscle mass depletion. Despite the detrimental consequences, there is no standardized and clinically-approved intervention currently available for cardiac cachexia. In the context of cardiac cachexia, accelerated protein turnover, that is, inhibited protein synthesis and enhanced protein degradation, plays a crucial role in skeletal muscle wasting. This process is primarily mediated by various proteins encoded by atrogenes. Among them, the atrogene Trim63 (tripartite motif family 63) and its encoded protein MuRF1 have been extensively studied. This review article aims to elucidate the pathogenic mechanisms underlying skeletal muscle wasting in cardiac cachexia, describe the biochemical characteristics of MuRF1, and provide an overview of the investigation into MuRF1-targeting inhibitors. The ultimate goal is to offer novel strategies for the clinical treatment for skeletal muscle wasting associated with cardiac cachexia.

Inter–Intraseasonality of Meteorological Drivers of Chorabari Glacier, Central Himalaya: Implications for Mass Fluctuations and Associated Hazards

Abstract High-altitude meteorological data are scarce in the Himalayan region, particularly for glacierized catchments. In-depth knowledge of meteorological variables is crucial to describe numerous cryospheric processes and associated hazards. The present study comprehensively assesses time series data (2013–16) of meteorological parameters in the Chorabari Glacier catchment (CGC) using an automatic weather station (∼4270 m MSL). Results revealed that average near-surface air temperature varied seasonally, ranging from 6.4°C in the summer monsoon (June–September) to −6.3°C in the winter season (December–February). The minimum temperature (Tmin) remained positive throughout the monsoon seasons in the higher elevations (∼5050 m MSL), indicating the melting of glaciers and their reduced mass. The daily relative humidity in different seasons fluctuated from 39% to 90%, whereas the highest seasonal average (∼63%) was observed in the monsoon season. The average wind speed was high (∼1.7 m s−1) during winter and dropped to 1.1 m s−1 in the monsoon season. The average daily influx of solar radiation ranged from 19 to 374 W m−2, with the highest value recorded during the premonsoon (March–May ∼ 212 W m−2) and the lowest during the winter (∼149 W m−2) season. The daily outflux solar radiation varied from 18 to 186 W m−2, with the maximum value in premonsoon (∼145 W m−2) and the minimum value in the monsoon season (∼30 W m−2). The mean zero-degree isotherm was found above the equilibrium line altitude (ELA ∼ 5120 m MSL) during the monsoon season, indicating a considerable mass depletion of the Chorabari Glacier. The abnormal variations in climatic variables on 16–17 June 2013 were signs of the Kedarnath disaster. Significance Statement The main objective of this study is to understand the variability of meteorological drivers at daily, monthly, and seasonal scales and how these changes may impact glacier mass and downstream hydrology. Our research revealed considerable variations in maximum temperature throughout the year and positive minimum temperature at higher elevations during monsoon months, which significantly control the glacier mass changes. Meteorological variables showing cyclic behavior and indicators of the “monsoon lowering phenomenon” are important in determining the timing and intensity of the seasonal shift. Investigation of abnormal changes in the climatic drivers in the 2013 Kedarnath disaster provides valuable insight into the glacier-related hazards and draws attention to decision-makers to create robust strategies to monitor the dynamic response of Himalayan glaciers.

Abstract B014: NF-kB Regulates Innate Immunity in the Muscle Microenvironment to Control Distinct States of Wasting in Pancreatic Cancer-Induced Cachexia

Abstract Cancer cachexia is a debilitating syndrome characterized by unintentional weight loss largely due to the depletion of adipose and skeletal muscle mass. Cachexia occurs in at least half of all patients with cancer, with increasing incidences in more advanced cases, and is estimated to be responsible for greater than 20% of all cancer related deaths. In pancreatic ductal adenocarcinoma (PDAC), which has a 5-year survival rate of 13%, the incidence of cachexia can be as high as 80% and is associated with poor disease outcomes. Mitigating the effects of cachexia has the potential to increase both quality of life and survival for patients with PDAC. However, there are currently no effective means of treating cachexia, thereby underscoring the need to further understand the pathology of this disease. Although cancer cachexia is classically characterized as a systemic inflammatory disorder, emerging evidence indicates that weight loss also associates with local tissue inflammation. We queried the regulation of this inflammation and its causality to cachexia by exploring skeletal muscle, whose atrophy strongly associates with poor outcomes. Using both a genetically engineered mouse model of PDAC- induced cachexia named KPP and patient samples, we show that cachectic muscle is marked by enhanced innate immunity. Accumulation of macrophages is regulated by an NF-kB activity that localizes to multiple cells within the muscle microenvironment. In addition, this accumulation of macrophages derives from an equal contribution of infiltrating monocytes and resident cells. Moreover, NF-kB activated cells and macrophages undergo a crosstalk; whereas NF-kB+ cells signal and recruit macrophages to inhibit muscle regeneration and promote atrophy, but interestingly at the same time can also protect myofibers, macrophages signal back to NF-kB+ cells to sustain an innate immune response in a feed-forward loop. Together, we propose that NF-kB functions in multiple cells in the muscle microenvironment to regulate innate immunity that both promotes and protects against muscle wasting in cancer. Citation Format: Denis C Guttridge, Benjamin R Pryce, Alexander Oles, Erin E Talbert, Katherine A Morgan, David A Mahvi, Michael C Ostrowski, Teresa A Zimmers, James G Tidball, David J Wang. NF-kB Regulates Innate Immunity in the Muscle Microenvironment to Control Distinct States of Wasting in Pancreatic Cancer-Induced Cachexia [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Advances in Pancreatic Cancer Research; 2024 Sep 15-18; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2024;84(17 Suppl_2):Abstract nr B014.

Assessing cachexia in obesity: contradiction or perfectly possible?

Existing definitions of clinically important weight loss in patients with cancer do not specifically address weight loss in patients who are obese at presentation. This review explores the clinical impact of weight loss and depletion of the skeletal muscle mass (i.e., criteria defining cancer cachexia), in patients with obesity. Overweight and obese BMI values are shown by many recent studies to pose a survival advantage in patients with cancers of advanced stage, when compared with BMI in normal and underweight ranges. The classification of cancer-associated weight loss has evolved, and current grading schemes evaluate the impact of weight across the range of BMI values. Weight loss is associated with mortality in patients with BMI more than 30 kg/m 2 , however this is to a much lesser degree than in patients with lower BMI values. Diagnostic imaging permits the precise assessment of skeletal muscle index (SMI) in patients with cancer, and it has been clearly shown that while usually quite muscular, obese patients can have profound muscle depletion (i.e., sarcopenia), independent of the presence of weight loss. Muscle depletion associates strongly with mortality in obese patients, as well as with complications of cancer surgery and systemic therapy. It would seem contradictory to diagnose concurrent obesity and cachexia, as these terms represent opposite ends of the weight spectrum. Weight loss can occur in anyone with cancer, however its priority for clinical management may be lesser in obese versus low body weight individuals. Sarcopenic obesity is strongly associated with a poor clinical outcome and deserves further research, diagnosis in clinical practice, and new strategies for mitigation.

Galaxies with grains: unraveling dust evolution and extinction curves with hydrodynamical simulations

Dust in galaxies is an important tracer of galaxy properties and their evolution over time. The physical origin of the grain size distribution, the dust chemical composition, and, hence, the associated ultraviolet-to-optical extinctions in diverse galaxies remains elusive. To address this issue, we introduce a model for dust evolution in the RAMSES code for simulations of galaxies with a resolved multiphase interstellar medium. Dust is modelled as a fluid transported with the gas component, and is decomposed into two sizes, 5 nm and 0.1 μm, and two chemical compositions for carbonaceous and silicate grains. This dust model includes the growth of dust by accretion of elements from the gas phase and by the release of dust in stellar ejecta, the destruction by thermal sputtering, supernovae, and astration, and the exchange of dust mass between the two main populations of grain sizes by coagulation and shattering. Using a suite of isolated disc simulations with different masses and metallicities, the simulations can explore the role of these processes in shaping the key properties of dust in galaxies. The simulated Milky Way analogue reproduces the dust-to-metal mass ratio, depletion factors, size distribution and extinction curves of the Milky Way. Galaxies with lower metallicities reproduce the observed decrease in the dust-to-metal mass ratio with metallicity at around a few 0.1 Z⊙. This break in the dust-to-metal ratio corresponds to a galactic gas metallicity threshold that marks the transition from an ejecta-dominated to an accretion-dominated grain growth, and that is different for silicate and carbonaceous grains, with ≃0.1 Z⊙ and ≃0.5 Z⊙ respectively. This leads to more Magellanic Cloud-like extinction curves, i.e. with steeper slopes in the ultraviolet and a weaker bump feature at 2175 Å, in galaxies with lower masses and lower metallicities. Steeper slopes in these galaxies are caused by the combination of the higher efficiency of gas accretion by silicate relative to carbonaceous grains and by the low rates of coagulation that preserves the amount of small silicate grains. Weak bumps are due to the overall inefficient accretion growth of carbonaceous dust at low metallicity, whose growth is mostly supported by the release of large grains in SN ejecta. We also show that the formation of CO molecules is a key component to limit the ability of carbonaceous dust to grow, in particular in low-metallicity gas-rich galaxies.

Thigh muscle mass evaluated by point-of-care ultrasound is associated with short-term mortality in patients with sepsis in the emergency department

Muscle mass depletion is associated with mortality and morbidity in various conditions including sepsis. However, few studies have evaluated muscle mass using point-of-care ultrasound in patients with sepsis. This study aimed to evaluate the association between thigh muscle mass, evaluated using point-of-care ultrasound with panoramic view in patients with sepsis in the emergency department, and mortality. From March 2021 to October 2022, this prospective observational study used sepsis registry. Adult patients who were diagnosed with sepsis at the emergency department and who underwent point-of-care ultrasounds for lower extremities were included. The thigh muscle mass was evaluated by the cross-sectional area of the quadriceps femoris (CSA-QF) on point-of-care ultrasound using panoramic view. The primary outcome was 28 day mortality. Multivariable Cox proportional hazard model was performed. Of 112 included patients with sepsis, mean CSA-QF was significantly lower in the non-surviving group than surviving group (49.6 [34.3–56.5] vs. 63.2 [46.9–79.6] cm2, p = 0.002). Each cm2 increase of mean CSA-QF was independently associated with decreased 28 day mortality (adjusted hazard ratio 0.961, 95% CI 0.928–0.995, p = 0.026) after adjustment for potential confounders. The result of other measurements of CSA-QF were similar. The muscle mass of the quadriceps femoris evaluated using point-of-care ultrasound with panoramic view was associated with mortality in patients with sepsis. It might be a promising tool for determining risk factors for mortality in sepsis patients in the early stages of emergency department.

The effects of oral nutritional supplements on functional outcomes and body composition in patients with solid cancer undergoing systemic chemotherapy: A pilot study.

10074 Background: Cancer cachexia is a complex syndrome characterized by significant weight loss and depletion of skeletal muscle mass. Despite its profound impact on the well-being of cancer patients, the effective management of cancer cachexia remains a challenging and underexplored aspect of oncology. This study aims to investigate the potential benefits of oral nutritional supplements (ONS) to cancer patients undergoing chemotherapy. Methods: This is single-arm, prospective pilot study. Patients with cancer cachexia undergoing chemotherapy were included. Patients received ONS twice a day for 8 weeks. The ONS contained 200 kcal, 22.5 gram of carbohydrates, 7.5 gram of fat, and 12.5 gram of protein per 200 mL. Primary endpoints were improvements of lean body mass and physical performance. The secondary endpoints were changes of health-care related quality-of-life (HRQoL), nutritional status, and gut microbiomes profiles. EORTC-QLQ-C30 questionnaire, body composition analysis, 4-meter-walk test, hand grip strength test, and fecal microbiome analysis were conducted at baseline and after 8 weeks. The bacterial 16S rRNA gene (V3 and V4 region) was amplified using PCR and sequenced on the Illumina MiSeq platform. The QIIME 2 pipelines were used to analyze the raw data. Results: From January 2023 to October 2023, total of 10 patients were included. There were no significant differences of the mean daily energy intake (p=0.62) and calf-circumferences (p=0.2) between baseline and after 8 weeks. However, participants exhibited a statistically significant improvement in hand-grip strength (p=0.002) and 4-meter walk test (p=0.021) after 8 weeks. In the body composition analysis using dual-energy X-ray absorptiometry, no differences were observed in body weight (p=0.43), fat mass (p=0.62), fat-free mass (p=0.27), and fat-free mass index (p=0.38). Also, no significant differences were detected in the results of HRQoL survey (p=0.85). In the microbiome analysis, no differences of microbiome composition at phylum and genus levels, α-diversity, and β-diversity were observed between baseline and after 8 weeks. Conclusions: While ONS have shown potential in enhancing physical functions of patients with cancer cachexia undergoing chemotherapy, this did not correlate with the anticipated microbiome changes or improvements in body composition. The absence of expected microbiome alterations, despite enriched protein and dietary fiber, underscores the complexity of cachexia and indicates that its management may extend beyond nutritional supplementation alone. Further research should explore the broader context of cachexia, including nutrition, metabolic processes, and the impact of chemotherapy.

Association between Muscle Mass Index and Neurological Outcomes of Patients with Out-of-Hospital Cardiac Arrest.

Muscle mass depletion is associated with unfavorable outcomes in many diseases. However, its relationship with cardiac arrest outcomes has not been explored. This retrospective single-center study determined the relationship between muscle mass depletion and the neurological outcomes of patients with out-of-hospital cardiac arrest (OHCA) by measuring muscle mass at various locations. Adult patients with OHCA, who were treated with target temperature management, and who underwent abdominal or chest computed tomography (CT) within 3 months of the cardiac arrest were included. Skeletal muscle index (SMI) was measured at the third lumbar vertebra (L3) level, psoas muscle, fourth thoracic vertebra (T4) level, and pectoralis muscle. The Youden index was used to determine a low SMI based on sex-specific cutoff values. The outcome variables were "good neurological outcome" and "survival" at hospital discharge. Multivariable analyses revealed that patients with low T4 SMI level were significantly associated with good neurological outcomes at hospital discharge (odds ratio = 0.26, 95% confidence interval: 0.07-0.88, p = 0.036). However, no significant differences were observed between good neurological outcomes and low SMI at the L3 level and psoas and pectoralis muscles; SMIs were not associated with survival at hospital discharge. T4 level SMI depletion was inversely associated with good neurological outcomes in patients with OHCA. Thoracic muscle depletion may be crucial for predicting the neurological outcomes in patients with OHCA and further investigation in larger prospective study is warranted.

A novel preclinical model of human lung cancer cachexia

Most patients with lung cancer are afflicted with cancer cachexia (CC), a syndrome characterized by wasting of muscle and fat tissues, which leads to poor treatment response and increased mortality. Pre-clinical models used to study CC often do not mimic the human condition, which may explain why there are no approved treatments for CC. To overcome this barrier, we sought to develop a model of lung CC that better recapitulates the pathoetiological characteristics of human lung CC using mice with a tamoxifen-inducible, lung epithelial cell (club cell)-specific KrasG12D allele ( G12D mice). We hypothesized that this model would mimic human CC in tumor location and burden, rate of progression, and tumor-driven tissue wasting. Following induction by tamoxifen injection, adenocarcinomas developed in the terminal bronchioles as early as 3.5 weeks post-induction and were accompanied by loss of 15% of animal body weight over a 12-week period. This weight loss began at 6 weeks and yielded a slow rate of CC progression (-1.9%/week) relative to currently available lung CC models (-5-10%/week). Body weight loss resulted from reductions in hindlimb muscle mass (18-30%) and profound depletion of inguinal and gonadal adipose tissue depots (60-70%) compared to age-matched wild-type (WT) littermates. At 6 weeks post-induction, fat tissue was reduced at the tissue and cellular levels while reductions in hindlimb muscle weight and fiber size were not observed. This fat loss corresponded with increased serum glycerol levels at 6 and 12 weeks, a metabolic characteristic that was also found in patients with lung cancer receiving treatment. Lung organoids were developed from G12D and WT mice. G12D organoids exhibited similarities in gene expression to human lung tumors ( RNAseq) and their conditioned medium (CM) elicited glycerol release from cultured 3T3L1 adipocytes compared to CM from WT organoids, suggesting that tumor-derived factors may contribute to early adipose tissue atrophy. Collectively, these findings suggest that G12D mice may be a valuable model, well-suited to identify mechanisms leading to CC and test preventative therapies. NIH R01 AR065826(MJT), NCI R21 CA283492(MJT), T32- HL076122-18(DBS/MEP), R01 CA273238 (YJ-H) and the University of Vermont Cancer Center. Funding support for RNAseq provided by Zymo Impact Initiative Grant (DBS). This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Investigations on Microstructure and Mechanical Properties of HAp Nanocomposites reinforced with ZrO2 and TiO2

In the contemporary context, approximately 15 million individuals in India grapple with bone-related issues stemming from accidents, fractures, bone mass depletion, and the natural aging process. To address this challenge, a diverse array of biomaterials has been innovated to rectify or enhance bone deficiencies within the human body. Nevertheless, over the past decade, calcium phosphate-based bioceramic materials have gained significant prominence as synthetic alternatives for bone and dental applications in the fields of orthopaedics and dentistry. One such bioactive ceramic, Hydroxyapatite (HAp), boasts exceptional versatility and can find utility in both dental and orthopaedic contexts. In the current research, exhaustive research was conducted to explore the impact of HAp/(ZrO2+TiO2) hybrid nano-composites synthesized through a mechanical synthesis process employing high-energy ball milling (HEBM), incorporating varying weight proportions. The resulting “HAp/(ZrO2+TiO2) nano-composites” were subjected to characterization through scanning electron microscopy combined with energy-dispersive X-ray spectroscopy (SEM-EDX) and X-ray diffraction (XRD). Furthermore, the study assessed the influence of these nano-composites regarding the HAp nano-composites’ mechanical characteristics.

Gastrin: a new branch of the gastropancreatic axis that can explain the effect of sleeve gastrectomy on glucose metabolism

BackgroundAmong bariatric techniques, sleeve gastrectomy (SG) stands out owing to its efficiency. The role of the stomach as a secretory organ of many substances, such as gastrin, related to insulin secretion is well known. Gastrin induces insulin release in isolated pancreatic islets, limiting somatostatin-14 intraislet release, and has been associated with blood glucose level improvement in diabetic models after SG. SG involves gastric resection along the greater curvature. This study aimed to determine the role of gastrin in glucose metabolism improvement after SG with the aid of the gastrin antagonist netazepide. MethodsIn 12 sham-operated, 12 SG-operated, and 12 SG-operated/netazepide-treated Wistar rats, we compared medium- and long-term plasma insulin, oral glucose tolerance test (OGTT) results, and plasma gastrin levels. In addition, gastrin expression was assessed in the gastric remnant, and the beta-cell mass was measured. ResultsSG induced a medium-term elevation of the insulin response and plasma gastrin levels without modification of the OGTT results. However, long-term depletion of the insulin response with elevated OGTT areas under the curve and plasma gastrin levels appeared after SG. Netazepide prevented the SG effect on these parameters. Gastrin tissue expression was greater in SG animals than in SG/netazepide-treated or control animals. The beta-cell mass was lower in the SG group than in the control or SG/netazepide group. ConclusionGastrin plays a central role in glucose improvement after SG. It stimulates a medium-term strong insulin response but also causes long-term beta-cell mass depletion and a loss of insulin response. These effects are prevented by gastrin antagonists such as netazepide.

Glacier Mass Balance and Its Impact on Land Water Storage in the Southeastern Tibetan Plateau Revealed by ICESat-2 and GRACE-FO

The southeastern Tibetan Plateau (SETP), which hosts the most extensive marine glaciers on the Tibetan Plateau (TP), exhibits enhanced sensitivity to climatic fluctuations. Under global warming, persistent glacier mass depletion within the SETP poses a risk to water resource security and sustainability in adjacent nations and regions. This study deployed a high-precision ICESat-2 satellite altimetry technique to evaluate SETP glacier thickness changes from 2018 to 2022. Our results show that the average change rate in glacier thickness in the SETP is −0.91 ± 0.18 m/yr, and the corresponding glacier mass change is −7.61 ± 1.52 Gt/yr. In the SETP, the glacier mass loss obtained via ICESat-2 data is larger than the mass change in total land water storage observed by the Gravity Recovery and Climate Experiment follow-on satellite (GRACE-FO), −5.13 ± 2.55 Gt/yr, which underscores the changes occurring in other land water components, including snow (−0.44 ± 0.09 Gt/yr), lakes (−0.06 ± 0.02 Gt/yr), soil moisture (1.88 ± 1.83 Gt/yr), and groundwater (1.45 ± 0.70 Gt/yr), with a closure error of −0.35 Gt/yr. This demonstrates that this dramatic glacier mass loss is the main reason for the decrease in total land water storage in the SETP. Generally, there are decreasing trends in solid water storage (glacier and snow) against stable or increasing trends in liquid water storage (lakes, soil moisture, and groundwater) in the SETP. This persistent decrease in solid water is linked to the enhanced melting induced by rising temperatures. Given the decreasing trend in summer precipitation, the surge in liquid water in the SETP should be principally ascribed to the increased melting of solid water.

Prognostic Impact of Preoperative Assessment of Muscle Mass and Strength in Surgically Resected Lung Cancer.

The association between resected non-small cell lung cancer (NSCLC) and long-term outcomes of muscle mass depletion and muscle weakness has also not been well documented. This study evaluated whether muscle mass depletion assessed by bioelectrical impedance analysis (BIA) and low muscle strength assessed by the peak expiratory flow rate as a percentage of predicted value (%PEFR) were associated with surgical outcomes in patients with resected NSCLC. This retrospective study included 219 patients with resected NSCLC between 2016 and 2021. The cutoff value for muscle mass depletion was according to guidelines, for low muscle strength, we defined by receiver operating characteristics analysis for recurrence-free survival (RFS). Survival analysis was performed, and postoperative outcomes were compared. A total of 76 patients (34.7%) had muscle mass depletion, and 114 patients (52.1%) had low muscle strength. Muscle mass depletion and low muscle strength were independent poor prognostic factors for overall survival [hazard ratio (HR)=2.631, p=0.003; HR=1.983, p=0.044] and RFS (HR=3.120, p<0.001; HR=1.857, p=0.028) in multivariate analysis. Postoperative complication was associated with low muscle strength (p=0.009). Postoperative recurrence was associated with muscle mass depletion (p=0.03). Preoperative muscle mass depletion assessed by BIA and low muscle strength determined by %PEFR are worse prognostic factors after surgical resection for NSCLC. Our results may provide some important information for preoperative management.

Long-term ice mass changes in Greenland and Antarctica derived from satellite laser ranging

Time-variable gravity field models obtained from satellite gravimetric techniques allow for the assessment of ice sheet mass changes in remote polar regions, such as Greenland and Antarctica. So far, GRACE has been the primary mission for obtaining the global time-variable gravity field models. However, GRACE was launched in 2002, thus very little is known about the global mass changes before this data, as well as between GRACE and its successor – GRACE Follow-On. We derive a method of gravity field recovery based on Satellite Laser Ranging (SLR) data to geodetic satellites that allows for obtaining direct ice mass change estimates for a period longer by 10 years than that provided by the GRACE missions. The developed method is based on splitting normal equation systems and re-stacking the solutions which allow for stable inversion, reduces the correlations between obtained parameters, stabilizes the ice mass estimates in polar regions, and reduces the noise over oceans by a factor of four. The secular trends obtained from SLR are equal to −113.5 and −82.8 Gt/year, whereas these are −119.1 and −83.3 Gt/year from GRACE and GRACE-FO to degree and order 10 for Greenland and West Antarctica, respectively, for the common period of 2002–2021 and after removing the post-glacial rebound effect. Despite the conformity of the trend and patterns, an underestimation is observed in the solutions expanded to degree and order 10. Therefore, scaling factors between GRACE/GRACE-FO expanded up to a degree and order 10 × 10 and 60 × 60 were derived and applied to SLR solutions to account for the differences in mass estimates due to the truncation of the models. SLR data revealed that in Greenland the smallest ice mass trends are for 1995–2000, 2000–2005, and 2015–2020 which are equal to +54.3, −15.5, and −75.9 Gt/year. The largest ice mass depletion periods took place in 2005–2010, 2010–2015, and recently in 2019–2021 with trends of −213.9, −287.2, and −276.1 Gt/year, respectively. For Greenland and West Antarctica, the period 2010–2015 is characterized by the most enormous ice depletion events, whereas the later 5-year period of 2015–2020 provided a near mass-equilibrium for Antarctica reducing the negative trend and returning to the situation from the ‘90s when no significant ice mass changes were observed.

Increased Serum Soluble (Pro)renin Receptor Levels in Patients with Sarcopenia

Purpose. Sarcopenia is an advancing and widespread skeletal muscle condition characterized by the depletion of skeletal muscle mass. The (pro)renin receptor ((P)RR) is a multifaceted protein with both pathophysiological and physiological functions. (P)RR is cleaved to generate soluble (P)RR, whose blood concentration may reflect the expression level of tissue (P)RR. We have discovered that the expression of (P)RR was elevated in the atrophied skeletal muscles of aged mice and humans. Methods. In this study, we examined the correlation between muscle atrophy and serum soluble (P)RR levels in bedridden patients with sarcopenia. Results. In total, 45 subjects were enrolled. Their ages were 81 (59–89) y.o., and serum soluble (P)RR levels were 30.5 ± 7.3 ng/ml. Lean body mass ratio (lean body mass/body weight), an index of muscle mass, was independently significantly and negatively correlated with serum soluble (P)RR concentrations in all patients. In addition, the lean body mass ratio was independently significantly and negatively correlated with serum soluble (P)RR concentrations in older patients (≥82 y.o.) but not in younger patients (&lt;82 y.o.). Conclusion. These data suggested that patients with sarcopenia, especially in elderly patients, may be associated with increased (P)RR expression. The serum soluble (P)RR level could be a biomarker of sarcopenia although this issue should be investigated by future studies.