Cosmetology Department Research Articles

In vitro cytotoxicity of the ternary PAMAM G3–pyridoxal–biotin bioconjugate

A third-generation polyamidoamine dendrimer (PAMAM G3) was used as a macromolecular carrier for pyridoxal and biotin. The binary covalent bioconjugate of G3, with nine molecules of biotin per one molecule of G3 (G39B), and the ternary covalent bioconjugate of G3, with nine biotin and ten pyridoxal molecules (G39B10P), were synthesized. The biotin and pyridoxal residues of the bioconjugate were available for carboxylase and transaminase enzymes, as demonstrated in the conversion of pyruvate to oxaloacetate and alanine to pyruvate, respectively, by in vitro monitoring of the reactions, using 1H nuclear magnetic resonance spectroscopy. The toxicity of the ternary bioconjugate (BC-PAMAM) was studied in vitro on BJ human normal skin fibroblasts and human squamous cell carcinoma (SCC-15) cell cultures in comparison with PAMAM G3, using three cytotoxicity assays (XTT, neutral red, and crystal violet) and an estimation of apoptosis by confocal microscopy detection. The tests have shown that BC-PAMAM has significantly lower cytotoxicity compared with PAMAM. Nonconjugated PAMAM was not cytotoxic at concentrations up to 5 μM (NR) and 10 μM (XTT), and BC-PAMAM was not cytotoxic up to 50 μM (both assays) for both cell lines. It has been also found that normal fibroblasts were more sensitive than SCC to both PAMAM and BC-PAMAM. The effect of PAMAM and BC-PAMAM on the initiation of apoptosis (PAMAM in fibroblasts at 5 μM and BC-PAMAM at 10 μM in both cell lines) corresponded with cytotoxicity assays for both cell lines. We concluded that normal fibroblasts are more sensitive to the cytotoxic effects of the PAMAM G3 dendrimer and that modification of its surface cationic groups by substitution with biologically active molecules significantly decreases that effect, confirming that PAMAM G3 is a useful candidate as a carrier for active biocompound delivery.

Research of nursing crisis management in medical cosmetology department

Objective To explore the causes of nursing crisis in medical cosmetology department and develop appropriated measurements,reduce the substantial risk of crisis events,and ultimately,improve the nursing quality.Methods Before the nursing crisis management implement,810 patients were collected from August 2009 to August 2010.After implement,1032 patients were collected from September 2010 to August 2011,Nursing deficiency rate,dispute rate between patients and nurses and satisfaction of patients were compared before and after implement of nursing crisis management.Results Nursing deficiency rate and dispute rate between patients and nurses were decreased from 4.2％ and 1.6％ to 2.2％ and 0.5％ after the implement of nursing crisis management.Satisfaction degree of patients increased from 86.9％ to 90.4％,and all the differences were statistically significant ( x2 =5.866,7.279 and 5.599 ; P ＜ 0.05 ).Conclusions Application of nursing crisis management in medical cosmetology department could reduce the substantial risk of crisis events,increase the anti-risk ability in cosmestic nursing,provide safe,high-quality nursing care and improve the satisfaction degree of patients. Key words: Cosmetology; Evaluation research; Nursing; Crisis management