Department Of Biology Research Articles

Flow cytometry for meningeal infiltration in B acute lymphoblastic leukemia in a low middle income country.

Meningeal infiltration in children with B acute lymphoblastic leukemia is one of the most serious complications. Timely diagnosis not only significantly enhances treatment efficacy but also leads to improve patient outcome and reduce risk of relapse. This is particularly crucial in low to middle income countries facing health constraints, where optimizing resources is essential. Conventional cytology (CC) study of cerebrospinal fluid (CSF) is considered in different countries to be the Gold-standard despite its low sensitivity (< 50%). The study of CSF by multiparametric flow cytometry (MFC) appears to be an alternative. The aim of our study was to assess MFC analytical performance compared with CC. Our cross sectional study was conducted over a six-month period in the biological hematology department. CSF samples underwent analysis for the presence of blasts using both CC and MFC. Cytological slides of the CSF were prepared by cytocentrifugation in a Shandon Cytospin 4™. Flow cytometric analysis was performed on the BD FACSLyric™ flow cytometer. All statistical analyses were performed using SPSS version 21.0 (SPSS Inc.). Agreement between the two methods was made using the Kappa index and χ2 test. This study was approved by the local ethics committee. Sixty CSF samples from 39 children with B acute lymphoblastic leukemia were analyzed. Meningeal infiltration was detected respectively in 20% of cases by MFC and 5% of cases by CC, with a significant difference p = 0.006. Comparing the two methods, the Kappa coefficient was 0.35, indicating weak agreement between the two methods. Moreover, MFC positivity was higher even for hypocellular samples. Of the 51 hypocellular samples, eight were positive by MFC while they were negative by CC. MFC shows better sensitivity while retaining good specificity for the detection of meningeal involvement. MFC could therefore be a complementary method to CC for detecting blast cells in the central nervous system.

Methodological aspects of using NIR spectroscopy to assess biochemical indicators in barley grain

Background. The possibility of applying near-infrared reflectance (NIR) spectroscopy for rapid assessment of various biochemical parameters in barley varieties and forms is discussed. The data obtained by the Biochemistry and Molecular Biology Department of VIR served to construct calibration models for the content of protein, starch, oil, beta-glucans, and total phenolic compounds (PhC) in grain, facilitating further screening of various barley samples.Materials and methods. The chemical composition of grain was studied in naked and covered spring barley (Hordeum vulgare L.) accessions grown in 2022 in the northwest of Russia. Calibration models were developed to measure the content of protein, oil, starch, beta-glucans, and PhC (80 accessions) in barley grain with the Matrix-I IR analyzer (Bruker Optics, Germany). The models were constructed on the basis of the data obtained by conventional techniques of chemical analysis. The protein/ nitrogen content was assessed using the Kjeldahl method, oil according to the method of defatted dry residue modified by S. V. Ruszkovsky, starch by the polarimetric method according to Evers, beta-glucans by gravimetric analysis, and the total PhC content by the Folin–Ciocâlteu method modified by Singleton and Rossi.Results. Statistical significance of the constructed calibration tests was compared with the results of measuring protein, starch, oil, beta-glucan and PhC levels by chemical methods. It was shown that the data of calibration techniques for protein and starch were significant, while the remaining models required improvement.Conclusion. The proposed method helps to preserve valuable source material, increases labor efficiency, and does not require chemical reagents. Scanning each sample makes it possible to obtain data for several indicators at once, with a specified replication and standard deviation.

A study of macrodontia of the permanent maxillary central incisors among Delta State University students in Abraka, Nigeria

Macrodontia (or megadontia) is a rare dental anomaly characterized by excessive enlargement of all tooth structures, and in few cases may be associated with morphological anomalies. The goal of this study was to find out how common macrodontia of the maxillary central incisors was among Delta State University students in Abraka. The Research and Ethical Committee of the Department of Human Anatomy and Cell Biology, Delta State University, Abraka, gave approval for this study. A descriptive cross-sectional survey with a representative sample of 102 subjects was conducted (58 female and 44 male). The method used was a simple random sampling technique. The age and sex of each subject were obtained using a data form, and intra-oral measurements of the mesio-distal width of the maxillary right central incisors were taken. Results were arranged according to age and sex to ascertain the occurrence of sexual dimorphism; independent sample t test and chi-square test were used as inferential statistical tools. The prevalence of macrodontia was 35.3%; there was a significant sex difference, as p value was &lt;0.05; there was no significant difference within the age groups, as p-value was &gt; 0.05. The mean mesio-distal width was 8.54±0.25 mm in those who did not have macrodontia and 9.63±0.54 mm in those who did have macrodontia. Macrodontia of central incisors is dominant amid Delta State University learners in Abraka.

Topics of Ecology Course in Preparing STEM-based Module

Ecology is a compulsory course for students of Bachelors’ Degree in Biology Department. The course lecture includes theories and practices. During the delivery of theories, content conveyed to the students must be in accordance with the course characteristics. Based on interview results, students considered some of the contents to be difficult. The research aims to select topics in Ecology course as a basis for developing STEM (Science, Technique, Engineering, Mathematics)-based module. The expectation is that students could gain a better understanding after the implementation of the STEM-based module approach. The research is qualitative research with a survey method. The data collection were done using survey questionnaire on Google Form platform. The data analysis technique was carried out by elaborating the questionnaire survey results regarding content requirement of the Ecology course. The research finds that several contents delivered in the Ecology course are deemed as difficult. The contents include biogeochemical cycles as well as energy and productivity. Keywords: ecology, course, STEM-based module

Long COVID: Epidemiology, post-COVID-19 manifestations, possible mechanisms, treatment, and prevention strategies – A review

Background and objectives: The respiratory disease COVID-19 began in 2019 and quickly became a pandemic infecting millions of individuals across the globe. Many patients show lingering effects of the infection several days after testing negative for the disease. This has become known as “long COVID” and is defined by various sources as lasting anywhere from 4 weeks to periods. This is a review of the existing literature on long COVID which offersextensive insights into its clinical features, diagnosis, and treatment. Materials and method: Information on clinical features, mechanisms, treatment options, preventive measures, and epidemiology of long COVID is derived from an extensive review of scientific journals and pertinent authoritative sources. Results: The virus enters the cells via angiotensin-converting enzyme 2(ACE2) receptors. ACE2 receptors are present on numerous cell types throughout the body and thus the virus can affect several organs resulting in avariety of different symptoms. Long COVID symptoms include fatigue, dyspnea, headache, brain fog, and symptoms related to cardiovascular and pulmonary systems. Fatigue can affect upwards of 93% of patients suffering from long COVID. Failure of the body to clear the virus could initiate this chronic effect. Studies indicate that the use of antiviral drugs at the early phase of COVID-19 could prevent long COVID symptoms. Vaccines against SARS-CoV-2 also might help prevent long COVID. Conclusion: Diagnosing and managing long COVID is challenging due to diverse symptoms, including mental health issues like anxiety and depression. Longitudinal studies and patient-oriented approaches are crucial for treatment, supported by policies and educational campaigns. Understanding the pathophysiology remains a top priority. July 2024; Vol. 18(2):003. DOI: https://doi.org/10.55010/imcjms.18.015 *Correspondence: M. S. Zaman, Department of Biological Sciences, Alcorn State University, Lorman, MS 39096, USA; Department of Biology, South Texas College, McAllen, TX 78501, USA. Emails: zaman@alcorn.edu; mzaman@southtexascollege.edu

Investigation of Parasites and Microbial Load in Local Beverages Sold by Vendors in the Port Harcourt Metropolis, Nigeria

Aim: To determine the prevalence and distribution of gastrointestinal parasites and microbes in Fura da Nono, Gbagaba (Washing and setting), Agbo-iba (Malaria) and Agbo-jedi jedi sold by street vendors in Rivers State. Study Design:  A total of 216 samples were procured randomly from eight different locations. Place and Duration of Study: Department of Animal and Environmental Biology [parasitology unit), University of Port Harcourt, from March - July 2022. Methodology: The 216 samples were examined for gastrointestinal parasites using the Concentration method, while 120 of the samples selected based on type and location were examined for bacteria and fungi using standard microbial techniques. Results: out of the 216 samples examined, 105(48.61%) local made drinks were contaminated with parasites as follows; Gbagagba (washing &amp; setting) 48(81.3%), Agbo jedi-jedi 24(44.4%), Agbo Iba 22(40.7%) and Fura da Nono 11(20.4%) with the following species of parasites; Hookworm 48(22.2%), Ascaris lumbricoides 34(15.7%), and Trichuris trichiura 23(10.6%) p=.05. Of the120 local drinks subjected to bacterial evaluation, 92(76.67%) were positive for bacteria as follows; Fura da Nono 26(86.7%), Agbo jedi-jedi 24(80%), Gbagagba 22(73.3%), and Agbo jedi jedi 20(66.7%), with the following species of bacteria; Staphylococcus aureus 49(40.83%), Escherichia coli 16(13.3%) and Klebsiella spp 27(22.5%) p=.05. while 73(60.8%) was positive for fungi as follows; Fura da Nono 22(73.3%), Agbo Jedi-Jedi 21(70%), Gbagagba 12(56.7%) and Ago-Iba 13(43.2%). Candida species were the only fungi observed in the drinks. This study has shown various microorganisms present in locally made drinks which could have resulted from contaminated soils or poor hygiene during preparation processes. Conclusion: Street vendors who produce these drinks should be regularly trained on safe handling of their products and send them to approved laboratories for quality assessment before marketing.

Comparison of oxidative stress, lipid peroxidation and inflammatory markers between rheumatoid arthritis and ankylosing spondylitis patients

Objective: To measure the levels of superoxide dismutase and malondialdehyde along with erythrocyte sedimentation rate and C-reactive protein in patients of rheumatoid arthritis and ankylosing spondylitis. Method: The comparative, cross-sectional study was conducted from February 2 to December 30, 2022, at the Centre for Research in Experimental and Applied Medicine laboratory of the Department of Biochemistry and Molecular Biology, Army Medical College, Rawalpindi, Pakistan, in collaboration with the Department of Rheumatology, Pak Emirates Military Hospital, Rawalpindi. The sample comprised healthy controls in group 1, patients of rheumatoid arthritis in group 2 and patients of ankylosing spondylitis in group 3. Blood samples were assessed for levels of superoxide dismutase, malondialdehyde, erythrocyte sedimentation rate and C-reactive protein. Data was analysed using SPSS 25. Results: Of the 180 subjects, 60(33.3%) were in group 1; 32(53.3%) females and 28(46.7%) males with mean age 34.9±6.4 years. There were 60(33.3%) patients in group 2; 35(58.3%) females and 25(41.7%) males with mean age 46.0±11.1 years. There were 60(33.3%) patients in group 3, and all 60(100%) were males with mean age 35.9±6.9 years. Superoxide dismutase level was significantly low and malondialdehyde level was significantly high in groups 2 and 3 compared to group 1 (p&lt;0.05). Erythrocyte sedimentation rate was the highest in group 2, followed by group 3 (p&lt;0.05). C-reactive protein levels were the highest in group 2 and the lowest in group 3 (p&lt;0.05). A significantly negative correlation (p&lt;0.001) was found between superoxide dismutase and malondialdehyde. Conclusion: Oxidative stress played a pivotal role in chronic inflammatory rheumatic diseases, like rheumatoid arthritis and ankylosing spondylitis. Key Words: Rheumatoid arthritis, Ankylosing spondylitis, Superoxide dismutase, Malondialdehyde.

Peptidoglycan modulation by Francisella tularensis

HAYDEN HESS, STUART CANTLAY, JOSEPH HORZEMPA, Department of Biomedical Sciences, West Liberty University, West Liberty, WV 26074 and MELANIE SAL, Department of Biology, West Virginia Wesleyan College, Buckhannon, WV 26201. Peptidoglycan modulation by Francisella tularensis.   Peptidoglycan, a crucial component of bacterial cell walls, plays a fundamental role in maintaining cell shape, integrity, and resistance to osmotic pressure. Mycoplasmas are the only bacteria that do not produce peptidoglycan cell walls.  Other peptidoglycan-producing bacteria are capable of transiently existing without a peptidoglycan as an “L-form”, but only on solid media.  Preliminary observations made by our laboratory suggested that Francisella tularensis bacteria do not produce detectible levels of peptidoglycan while growing in liquid media.  However, increasing salt concentration induces the production of peptidoglycan in all bacteria.  In this study we confirmed and extended these observations by incubating bacteria with fluorescent D-alanine (which integrates into the peptidoglycan during synthesis).  Fluorescence microscopy revealed that very few F. tularensis bacteria produce detectable peptidoglycan in low salt media, while seemingly all individual bacteria produce this layer in high salt media.  Interestingly, the genome of F. tularensis encodes two distinct beta-lactamases.  Here we show that both the wild-type F. tularensis LVS and beta-lactamase null mutant were both resistant to high levels of beta-lactam antibiotics in low salt liquid media.  However, only the wild type and complemented mutant strains were resistant to beta-lactam antibiotics in high salt media while the beta lactamase null mutant was susceptible.  These data further support the conclusion that F. tularensis does not produce, nor need, peptidoglycan in low salt liquid media.  Future studies will investigate whether peptidoglycan modulation is required to avoid detection by the innate immune system.

ECR Spotlight – Mads Kuhlmann Andersen

ECR Spotlight is a series of interviews with early-career authors from a selection of papers published in Journal of Experimental Biology and aims to promote not only the diversity of early-career researchers (ECRs) working in experimental biology but also the huge variety of animals and physiological systems that are essential for the ‘comparative’ approach. Mads Kuhlmann Andersen is an author on ‘ The freeze-avoiding mountain pine beetle (Dendroctonus ponderosae) survives prolonged exposure to stressful cold by mitigating ionoregulatory collapse’, published in JEB. Mads Kuhlmann conducted the research described in this article while a postdoc in Heath MacMillan's lab at Department of Biology, Carleton University, Ottawa, ON, Canada. He is now a postdoc in the lab of Johannes Overgaard at Department of Biology, Aarhus University, Denmark, investigating comparative physiology and integrative biology with a main interest in how animals respond, adjust and adapt to different thermal environments.

Predictors of Global Scientific Literacy of Pre-Service Teachers: A Structural Equation Modeling Study

The purpose of this study was to examine the relationship among pre-service science teachers' global scientific literacy and various variables within the scope of the structural equation model. Since the relations among the variables were investigated in the structural model established for this study, correlational research methodology was applied. The application was carried out with 294 pre-service teachers studying in the departments of chemistry, physics, biology and elementary science education of a state university. The data of the study were obtained with the scales of global scientific literacy, the perception of science process skills, the inquiry skills, the self-directed learning readiness in laboratory, the attitude scale towards the laboratory and the personal information questionnaire. Within the framework of structural equation modeling, the causal and relational analyses of the data were examined. The results showed that the proposed model of the relationship among global scientific literacy and the variables predicting global scientific literacy was also found to be compatible with the data, and the entire model was confirmed except for the attitude towards the laboratory. In this context, it can be claimed that these variables play an essential role in pre-service teachers’ being global scientifically literate.

Morphometric studies of the skin of calfs

Relevance. Previously, we conducted comparative morphometric studies of skin samples from different topographic sites in adult cows obtained in winter. At the same time, morphological features of the skin of different topographic areas were established. In this paper, the purpose of our study was to conduct comparative morphometric studies of the skin of different topographic areas in calves.Methods. Morphometric studies of histological preparations were carried out at the Department of Pathological Anatomy and Forensic Veterinary Medicine and at the Department of Biology, Ecology and Histology. Morphometric studies of histological preparations were used to study the thickness of the dermis proper, as well as bundles of collagen fibers of the mesh layer of the dermis. The research results were statistically processed using the "Biostat" program.Results. Morphological features of the skin of different topographic areas in calves have been established, manifested by different thicknesses of skin layers and bundles of collagen fibers of the dermis. The morphometry performed made it possible to supplement the data on the morphological structure of the skin of cattle and can also be used in the study of morpho- and pathogenesis of skin diseases.

The phenetic relationship of citrus plants based on the morphological and anatomical characteristics

Abstract. Nurzaman M, Setiawati T, Hasan R, Qotrunnada NK, Kusmoro J, Permadi N, Julaeha E. 2024. The phenetic relationship of citrus plants based on the morphological and anatomical characteristics. Biodiversitas 25: 1201-1213. Indonesia stands as a global biodiversity hub, boasting an exceptional richness that earns it the distinction of being a mega-biodiversity country. Among its diverse ecosystems, the Rutaceae family holds significance, encompassing numerous genera, notably the Citrus genus, which showcases a splendid array of citrus plants. This research aimed to determine phenetic relationships based on the morphological and anatomical characters of seven citrus plants (Citrus reticulata var. Chokun, C. reticulata var. Fremont, C. reticulata var. Santang, C. reticulata var. Dekopon, C. aurantium var. variegata, C. aurantium, and C. limon) in Parongpong District, West Bandung Regency, West Java. The method used in this research was explorative with purposive sampling. This study collected three samples for respective citrus plants for character observations of leaf morphology, anatomy, and fruit morphology. Morphology and anatomy observations were conducted in the Department of Biology, Padjadjaran University laboratory. The description of morphological and anatomical data obtained from each orange plant was then analyzed using NTSYS PC version 2.11a software to obtain similarity values using the dendrogram analysis method. Phenetic analysis of seven orange plants produced a dendrogram divided into 2 main branches, branch I and branch II, with a similarity coefficient of 0.3846. Branch I consists of Chokun orange (Citrus reticulata var. Chokun), Fremont orange (C. reticulata cv. Fremont), Santang orange (C. reticulata cv. Santang), and variegated orange (C. sinensis cv. variegata) and branch II consists of dekopon orange (C. reticulata var. Dekopon), Sunkist orange (C. aurantium), and lemon (C. limon).

Developing Supplementary Books on Mangroves in Aceh Tamiang for Biology Students

This study aimed to produce supplementary books on mangroves in Aceh Tamiang for undergraduate students in the Ecology course. Research and Development (R&amp;D) adapted from the Thiagarajan (4D) model consisting of defining, designing, developing and disseminating stages were used. Field research was employed to develop the product. Material expert lecturers, learning design experts, and layout design experts validated the supplement book developed. Ecology lecturers and students of the Biology department at the State University of Medan assessed it. The results of the assessment by the material experts had a mean score of 86.6% in the very good/feasible category. Meanwhile, learning design experts had a mean score of 93.2% in the very good/feasible category. Likewise, the layout design experts had a mean score of 91.3% in the very good/feasible category. Responses towards the books by lecturers had a high very good/feasible category with an overall percentage of 90.26%. The students’ results based on individual, small group and limited field tests obtained the mean scores of 97.9%, 89% and 90.95%, respectively, in the very good/feasible category. These results indicated that the supplementary books that have been developed were very good/feasible. This product’s effectiveness could be seen from the gain score of 0.52 in the moderate category and the results of the Wilcoxon test t-count 0.000 &lt; t-table 0.05. It can be concluded that using the supplement books effectively increases students’ knowledge of mangrove ecosystems with significant differences in pretest and posttest scores so that supplement books can be used as additional teaching materials for lecturers, students and readers.&#x0D; Keywords: supplementary books, mangroves, ecology course

Transitions in development – an interview with Rajendhran Rajakumar

Rajendhran Rajakumar is a cross-appointed Assistant Professor in the Department of Biology and Department of Cellular and Molecular Medicine at the University of Ottawa, Canada. Rajendhran's lab uses multiple integrative approaches to understand how environmental factors act on evolution and development (eco-evo-devo) and how this leads to the evolution of biodiversity. We spoke to Rajee over Teams to learn more about his passion for emerging and model systems, most notably ants, and the importance of serendipity in research.

Optimization of Multiplex-PCR Technique To Determine Azf Deletions in infertility Male Patients.

To optimize the multiplex polymerase chain reaction (M-PCR) technique to diagnose microdeletions of azoospermia factors (AZF) on the Y chromosome and initially apply the technique to diagnose male patients with sperm density less than 5×106 million sperm/mL was assigned to do a test to check for AZF microdeletions on the Y chromosome. Based on the positive control samples which belong to male subjects who have had 2 healthy children without any assisted reproductive technologies, the M-PCR method was developed to detect simultaneously and accurately AZF microdeletions on 32 male patients with sperm densities below 5×106 million sperm/mL of semen at the Department of Biology and Medical Genetics - Vietnam Military Medical University. Successful optimization of the M-PCR technique including 7 reactions arranged according to each AZFabc region using 24 STS/gene on the Y chromosome. Initial application to diagnose AZF deletion on 32 azoospermic and oligospermic men reveals that AZFa deletion accounts for 6.25% (2/32); deletion of all 3 regions AZFa,b,c with 18.75% (6/32 cases); The combined deletion rate of AZFb,c is highest, accounting for 56.24% (18/32 patients). Successfully optimized the M-PCR technique in identifying AZF microdeletions using 24 sequence tagged sites (STS)/gene for azoospermic and oligozoospermic men. The M-PCR technique has great potential in the application of AZF deletion diagnosis.

The 2024 IUPAC-Richter Award Goes to Craig M. Crews

Abstract Craig M. Crews has been awarded the 2024 IUPAC-Richter Prize in Medicinal Chemistry. Crews is a Professor in the Department of Molecular, Cellular and Developmental Biology and Professor of Chemistry and Pharmacology at Yale University. His discovery and implementation of the PROteolysis TArgeting Chimeras (PROTACs) technology for removing specific unwanted proteins revolutionized the field by identifying a new method for affecting protein function. The fact that this technology has been widely adapted and investigated not only within the pharmaceutical industry, but also in academia, demonstrates the significance and impact of this work. While no drugs have yet been approved, several are in development and these molecules are progressing, because they are effective and safe. He also played a key role in the discovery of carfilzomib, an approved proteasome inhibitor for the treatment of multiple myeloma. The acceptance lecture will be held in Rome, Italy (1-5 September 2024) at the XXVIII EFMC International Symposium on Medicinal Chemistry.

An interview with Sandra Schmid – Chief Scientific Officer of the Chan Zuckerberg Biohub

ABSTRACT Sandra Schmid is the Chief Scientific Officer of the Chan Zuckerberg Biohub in San Francisco, USA. She was previously Professor and Chair of the Cell Biology Department at the University of Texas Southwestern Medical Center, and Chair of the Department of Cell Biology at Scripps Research Institute, CA, USA. Sandra's research over the years has focused on the biochemical dissection of the endocytic pathway, in particular the role of dynamin and the mechanisms of clathrin-mediated endocytosis. We chatted to Sandy to find out more about her career, her role as an advocate for women in science, and her recent transition to the Chan Zuckerberg Biohub.

Memories of Professor François Gros.

I joined the laboratory of Professor Francois Gros in 1987 and worked there as a postdoc with Robert Whalen until 1992. I recount the research we carried out and mention that of the other scientists also working on skeletal muscle on the 6th floor of the Molecular Biology Department of the Institut Pasteur at that time. I then present my subsequent research when I returned to Japan. I pay tribute to the influence of Professor Gros and to his support in establishing Japanese/French meetings on muscle biology and muscular dystrophy. I also invoke personal memories of Robert Whalen and Margaret Buckingham and remember the occasions when I returned to Paris to honour François Gros.

Needs Analysis of Education for Sustainable Development-Oriented Textbooks in Biotechnology Courses

Sustainability competencies can be developed through Education for Sustainable Development (ESD) whose implementation will be helped by appropriate teaching materials. The purpose of this study is to determine the perceptions and initial knowledge of students, conditions, and difficulties experienced, as well as the form of teaching materials that can facilitate students to achieve competence in biotechnology courses. This descriptive quantitative research was conducted on 30 students who had taken biotechnology courses at the Department of Biology, Universitas Negeri Malang purposively. Data collection using survey techniques and review of Semester Learning Plan documents. The survey results were analyzed descriptively using percentages. The results showed that 83.3% of students ranked biotechnology in the field of biorefinery as the most difficult material to understand in biotechnology, 83.3% of students thought that textbooks were teaching materials that were considered to make it easier to understand biotechnology material, and the main problem felt by 53.3% of students while undergoing biotechnology courses was difficulty in accessing appropriate teaching materials. In addition, the low number of students (13.3%) who apply the knowledge they have obtained during college to support sustainability in the community around their place of residence. So, it can be concluded that ESD-oriented textbooks with material content of fermentation technology applications in the biorefinery field need to be developed.

Implementing the Concept of Integration of Science and Islamic Values at Islamic University

The integration of scientific principles within Islamic religious universities governed by the Ministry of Religion is a widespread practice. However, the application of this concept across universities offering programs under the auspices of both the Ministry of Education and the Ministry of Religion remains unexplored. This study delineates the framework for integrating scientific knowledge at an Islamic University in Madura, focusing on the execution and assessment of education that amalgamates scientific insights and Islamic values. Employing observational techniques on policy documents and teaching resources of instructors, alongside semi-structured interviews with fifteen educators from the physics, biology, and mathematics departments, this investigation reveals a unique five-pillar knowledge integration concept. These pillars encompass Islam, Indonesia, Islamic Boarding School, Madura, and Aswajaan perspectives. The formal documentation supporting this integration at Madura Islamic University is encapsulated within its Statutes and chancellor's guidelines for curriculum enhancement. Evidence of implementation is observed in instructors' Syllabi and teaching materials, albeit only 53.3% have successfully linked scientific content with Qur'anic verses. The primary challenge identified is the considerable time required to develop materials that effectively integrate Islamic values. Solutions proposed include the creation of a comprehensive guide for integrating the five knowledge pillars and extensive training for all faculty members to facilitate this integration process.