Department Of Agriculture Research Articles

Thermal Inactivation of Cells of Salmonella spp. in Pot Pies Prepared With a Beef, Chicken, or Meat Alternative Filling, With and Without Gravy, During Cooking in a Convection Oven

Cooking parameters elaborated in the U.S. Department of Agriculture’s Food Safety and Inspection Service Cooking Guideline for Meat and Poultry Products (Appendix A) were evaluated for inactivation of Salmonella spp. in pot pies. To prepare dough for pot pies, flour, butter, sugar, salt, and water were mixed, portioned into balls (65 or 85 g each), flattened (ca. 13 or 15 cm diameter, ca. 0.5 cm thick), and hand-pressed into pans (ca. 19.4 cm diameter). Next, a 100-g portion of beef, chicken, or a meat alternative, with or without added gravy (55 g of protein and 45 g of gravy), was inoculated with a cocktail of Salmonella spp. (ca. 6.5 or 7.9 log CFU/g of filling) and distributed onto the pie crust. After covering with a 65-g sheet of dough, pies were heat sealed in nylon polyethylene bags and stored at −20 °C for up to 72 h. Frozen pot pies containing a beef or meat alternative filling were cooked in a convection oven to an internal temperature of 57.8 °C (136°F) instantaneous, 62.8 °C (145°F) and held for 4 min, 67.2 °C (153°F) and held for 34 sec, or 71.1 °C (160°F) instantaneous; whereas chicken pot pies were cooked to an internal temperature of 57.8 °C (136°F) instantaneous, 62.8 °C (145°F) and held for 13 min, 67.2 °C (153°F) and held for 96 sec, or 73.9 °C (165°F) instantaneous. Cells of Salmonella spp. were recovered from uncooked or cooked pot pies by stomaching each pie in peptone water and enumerating pathogen levels via direct plating. Cooking delivered Salmonella spp. reductions of ca. 3.6 to ≥6.3 log CFU/g of pot pie. With few exceptions, when pathogen levels decreased to below detection by direct plating (0.5 log CFU/g of pot pie), cells of Salmonella were not recovered by enrichment. In addition, there were minimal differences in the aw and moisture content of the protein filling before and after cooking, suggesting that enclosing the meat, poultry, or meat alternative filling within a dough wrapping maintains moisture in the filling during the cooking process.

A National Study of the Associations between Hormonal Modulators in Hydraulic Fracturing Fluid Chemicals and Birth Outcomes in the United States of America: A County-Level Analysis.

Risk of preterm birth (PTB) and low birth weight (LBW) due to hydraulic fracturing (HF) exposure is a growing concern. Regional studies have demonstrated links, but results are often contradictory among studies. This is the first US national study to our knowledge linking fracturing fluid ingredients to the human hormone pathways targeted-estrogen, testosterone, or other hormones (e.g., thyroid hormone)-to assess the effect of HF ingredients on rates of PTB and LBW. We constructed generalized linear regression models of the impact of HF well density and hormone targeting chemicals in HF fluids (2001-2018) on the county-level average period prevalence rates of PTB and LBW (2015-2018) with each outcome measured in separate models. Our data sources consisted of publicly available datasets, including the WellExplorer database, which uses data from FracFocus, the March of Dimes Peristats, the US Census Bureau, the US Department of Agriculture, and the Centers for Disease Control and Prevention. We conducted additional stratified analyses to address issues of confounding. We used stratification to address issues regarding outcomes in rural vs. urban communities by assessing whether our models achieved similar results in nonmetro counties, as well as farming and mining counties. We also stratified by the year of the HF data to include HF data that was closer to the time of the birth outcomes. We also added covariate adjustment to address other important factors linked to adverse birth outcomes, including the proportion of the population belonging to various racial and ethnic minority populations (each modeled as a separate variable); education (bachelor's degree and high school); use of fertilizers, herbicides, and insecticides, acres of agricultural land per square mile; poverty; insurance status; marital status; population per square mile; maternal care deserts; and drug deaths per 100,000 people. We found that the density of HF wells in a county was significantly associated with both PTB and LBW rates (percentage of live births) in our fully adjusted models. We report the results from our more restrictive stratified analysis with a subset including only the 2014-2018 data, because this resulted in the most meaningful time frame for comparison. Across all models, the magnitude of effect was highest for wells with ingredients that include estrogen targeting chemicals (ETCs), testosterone targeting chemicals (TTCs) and other hormone targeting chemicals (OHTCs), and, finally, all wells grouped regardless of chemical type. For every unit increase in well density per square mile of wells that use chemicals that include an ETC, we observed a 3.789-higher PTB rate (95% CI: 1.83, 5.74) compared with counties with no ETC wells from 2014 to 2018 and likewise, we observed a 1.964-higher LBW rate (95% CI: 0.41, 3.52). Similarly, for every unit increase in well density per square mile of wells that use TTC, we observed a 3.192-higher PTB rate (95% CI: 1.62, 4.77) compared with counties with no TTC wells. Likewise, for LBW, we found a 1.619-higher LBW rate (95% CI: 0.37, 2.87). We also found that an increase in well density per square mile among wells that use chemicals that include an OHTC resulting in a 2.276-higher PTB rate (95% CI: 1.25, 3.30) compared with counties with no OHTC wells, and for LBW, we found a 1.244-higher LBW rate (95% CI: 0.43, 2.06). We also explored the role of HF well exposure in general (regardless of the chemicals used) and found that an increase in total well density (grouped regardless of hormonal targeting status of the chemicals used) resulted in a 1.228-higher PTB rate (95% CI: 0.66, 1.80) compared with counties with no wells, and for LBW, we found a 0.602-higher LBW rate (95% CI: 0.15, 1.05) compared with counties with no wells. We found similar results in our primary analysis that used all data without any exclusions and the statistical significance did not change. Our findings reinforce previously identified regional associations between HF and PTB and LBW, but on a national scale. Our findings point to dysregulation of hormonal pathways underpinning HF exposure risk on birth outcomes, which warrants further exploration. Future research must consider the specific ingredients used in HF fluids to properly understand the differential effects of exposure. https://doi.org/10.1289/EHP12628.

Hot topic: Epidemiological and clinical aspects of highly pathogenic avian influenza H5N1 in dairy cattle

On March 24, 2024, the US Department of Agriculture (USDA) confirmed the first case of highly pathogenic avian influenza (HPAI) H5N1 clade 2.3.4.4b in dairy cattle. Following this initial detection, the virus was found to have spread to other species, including humans. Since then, at least 192 dairy herds across 13 states have tested positive for the virus. The emergence of HPAI H5N1 in dairy cattle poses a serious threat to the dairy industry, requiring an approach that integrates human, animal, and environmental health. Enhancing biosecurity, improving surveillance, and advancing research are critical to managing this challenge. Swift adaptation is needed to protect both public health and dairy production. Through this work, we discuss the epidemiological and clinical aspects of HPAI H5N1 in cattle by reviewing the spatiotemporal situation of the disease across the United States, the clinical signs and testing leading to a confirmed case, and the surveillance and control measures implemented at the national and state levels. Further, considering pathogenesis and risk factors, we discuss surveillance and control measures to implement within herds, and note areas where research is needed to understand how to control this emerging disease.

Development and Outlook for Self-priming Pumps

Background: Self-priming pumps are a type of general mechanical equipment that is widely used in the petroleum, chemical, electric power, metallurgy, mining, shipbuilding, light industry, agriculture, civil defense, and other departments, and they play an important role in the global economy. The pump system is the most common piece of equipment that consumes the most electricity, and reducing the energy consumption of the pump will save a significant amount of energy. Objective: For the benefit of scholars and engineers, this paper summarizes the most recent research on self-priming pumps, analyzes their solutions to the problem of reducing energy consumption, and discusses their advantages and disadvantages. Methods: The typical self-priming pump patents are outlined below based on the many functions of the improved self-priming pump structure, including pump body protection, high efficiency, and energy savings, ease of movement, and improved seal. The current self-priming pump patents are used to examine the development trend for these pumps. Results: The fundamental issues with self-priming pumps are outlined and studied through comparisons of various types and functions, and a future course for their development is suggested. Conclusion: The development of self-priming pump technology benefits from the improvement of self-priming pump structure. A self-priming pump with a straightforward design, automation, high efficiency, and energy conservation has a promising future.

SNAP-Ed Supporting Innovative Nutrition Education for American Indian and Indigenous-Serving Organizations in Colorado

BackgroundSNAP-Education (SNAP-Ed) is the nutrition education component of the United States Department of Agriculture (USDA) Supplemental Nutrition Assistance Program (SNAP). ObjectiveTo elucidate the experiences of three Colorado-based, American Indian or Indigenous-led organizations who engaged in a SNAP-Ed pilot funding project in FY2022. MethodsAn instrumental, single case-study design was employed to conduct this evaluation. The multi-phase method evaluation included: listening sessions (n=8 Indigenous-serving organizations), invited request for proposals, pilot award funding (n=3 awarded organizations), and longitudinal, storytelling-based qualitative data collection during the funding cycle. ResultsThere are many ongoing food and nutrition education programs serving Indigenous/American Indian peoples in Colorado, which largely promote wellness and healing through traditional cultural practices and are not overtly framed as “nutrition education.” Two salient themes emerged: 1) defining and describing Indigenized, decolonized nutrition education; and 2) description of experiences working with SNAP-Ed funding during FY2022, including challenges and future opportunities. ConclusionsTo effectively address the priority area for SNAP-Ed funding to reach Tribal communities in each state, understanding the perspectives of Tribal and Indigenous-led communities who are new to SNAP-Ed funding is prudent. Next steps to continued co-creation of SNAP-Ed opportunities in Indigenous spaces include educating funders and policymakers on Indigenous ways of knowing; traditional food and nutrition practices; and importance of relationality, land, healing, and other Indigenous values. Determining the “success” of a program (e.g., program evaluation) needs to be defined by the community itself.

Phenological Adaptation Is Insufficient to Offset Climate Change-Induced Yield Losses in US Hybrid Maize.

Climate change is projected to decrease maize yields due to warmer temperatures and their consequences. Studies using crop growth models (CGMs), however, have predicted that, through a combination of alterations to planting date, flowering time, and maturity, these yield losses can be mitigated or even reversed. Here, we examine three assumptions of such studies: (1) that climate has driven historical phenological trends, (2) that CGM ensembles provide unbiased estimates of yields under high temperatures, and (3) that the effects of temperature on yields are an emergent property of interactions between phenology and environment. We used data on maize phenology from the United States Department of Agriculture, a statistical model of maize hybrid heat tolerance derived from 80 years of public yield trial records across four US states, and outputs of an ensemble of CMIP6 climate models. While planting dates have advanced historically, we found a trend toward later planting dates after 2005 and no trend for silking or maturity, shifting more time into the reproductive period. We then projected maize yields using the historical model and crop calendars devised using three previously proposed adaptation strategies. In contrast to studies using CGMs, our statistical yield model projected severe yield losses under all three strategies. Finally, we projected maize yields accounting for historical genetic variability for heat tolerance, discovering that it was insufficient to overcome the negative effects of projected warming. These projections are driven by greater heat stress exposure under all crop calendars and climate scenarios. Combined with analysis of the internal sensitivities of CGMs to temperature, our results suggest that current projections do not adequately account for the effects of increasing temperatures on maize yields. Climate adaptation in the US Midwest must utilize a richer set of strategies than phenological adaptation, including improvements to heat tolerance and crop diversification.

The Adoption and Impact of Climate-Smart Water Management Technologies in Smallholder Farming Systems of Sub-Saharan Africa: A Systematic Literature Review

Agriculture plays a significant role in global water consumption, accounting for approximately 70% of the world’s freshwater usage. This makes this sector a critical factor in the depletion of water resources. Accordingly, this paper explores potential mitigatory impacts of climate-smart water management (CSWM) technologies in sub-Saharan Africa. About 70% of the population in sub-Saharan Africa is dependent on agriculture for sustaining their livelihoods. This is despite the low agricultural output in smallholder farming systems (SFS) due to water scarcity. This has spurred several attempts to promote the adoption of climate-smart agriculture (CSA) to raise agricultural outputs and improve smallholder farmers’ livelihoods. However, there has not been a comprehensive analysis of data categorised by various aspects of climate-smart water management technologies. In this systematic literature review, climate-smart water management technologies in sub-Saharan Africa’s agricultural sector were identified and analysed to determine strategies that could enhance their adoption and impact. To this end, academic articles reporting on the adoption of climate-smart water management technologies in databases were reviewed. Four significant literature databases were used. These were limited to Springer Link, ScienceDirect, MDPI, Wiley Online, and Google Scholar. The findings demonstrate that rainwater harvesting and micro-irrigation are the primary climate-smart water management technologies used by smallholder farmers. The literature review shows that adoption of CSWM practices is constrained by inadequate technological infrastructure, financial implications, unsuitable policies, and low user skills, particularly. It is therefore recommended that government agricultural departments and relevant advocates of CSA should incentivise and subsidise smallholder farmers to encourage CSWM technology adoption. This can be achieved through the implementation of suitable policies directed at technological infrastructure development, financial support for adoption, and technical skills training.

Correlation between Farmers' Activeness in Farmer Groups with Productivity and Income of Cocoa Farming in Trenggalek Regency

The cocoa plantation in Suruh Village, Trenggalek Regency is the main supplier of cocoa beans to UPH Rumah Coklat owned by the Department of Agriculture and Food of Trenggalek Regency. For example, in cocoa farmers' participation in the "Tunggal Jaya" Farmers Group, farmers are taught to make rorak and worm palaces to deal with climate change which helps maintain soil moisture and increase fertility. The objectives of this study are to determine 1) the level of farmer activeness, 2) the level of productivity, 3) the level of income, and 4) the correlation between farmer activeness in farmer groups with productivity and income of cocoa farming in Suruh Village, Trenggalek Regency. The number of samples used was 111 respondents. Data analysis used the Likert scale, productivity analysis, income analysis, and Pearson correlation. The results of the study showed: 1) cocoa farmer activeness in farmer groups in Suruh Village, Trenggalek Regency is in the active category with an overall average score of 3.56; 2) the average productivity of cocoa farming is in the medium category at 0.54 tons/ha/year; 3) the average income of cocoa farming is Rp6,016,562/year; 4) the correlation between farmer activeness with productivity is 0.865, farmer activeness with income is 0.760, and productivity with income is 0.868. The direction of the three correlations is positive and significant.

Evaluation of new sorghum varieties for grain production cultivated in mineral soil

Sorghum bicolor L. (Sorghum) has been recognized as the fifth largest cereal crop in the world, following maize, wheat, rice, and barley, with a mean annual production of more than 5.89 million tons over the past five years. Grain sorghum is one of the cultivated varieties of the sorghum genus. It is primarily used as human food and animal feed due to the beneficial effects of bioactive compounds in the grain. Sorghum is less popular and unrecognizable in Malaysia, as it was not included in the reports by the Agriculture Department regarding plantation areas. In Malaysia, sorghum is limited because this crop is considered new, and no studies were conducted on the adaptability of its varieties to Malaysian soil and local climatic conditions. Crop production practices such as variety selection, planting density and fertilization will affect yield production. Therefore, a study on grain sorghum varieties (Brachy Sorgho, Jumbo Super, India and Pacific Seed 99) was conducted to evaluate the growth and yield performance of sorghum. Jumbo Super had a significantly higher value for plant height, followed by Brachy Sorgho, India and Pacific Seed 99. India showed the highest value of leaf fresh and dry weight, but no significant difference compared with Brachy Sorgho. Brachy Sorgho recorded the highest value of stem fresh and dry weight, while the least was recorded for Pacific Seed 99. Brachy Sorgho and India showed significantly higher grain yields than Jumbo Super and Pacific Seed 99 in terms of panicle length, panicle weight without grain, grain weight without panicle per plant, number of grains per panicle, and 100-grain weight. The highest gross weight of grain sorghum is Brachy Sorgho (140.00 g/plant), which showed no significant difference compared to the India variety (131.13 g/plant). Meanwhile, Jumbo Super and Pacific Seed 99 varieties showed the lowest grain weight, 77.27 g/plant and 80.16 g/plant, respectively. Overall, all four varieties are adaptable in mineral soil and are considered potential alternative crops for grain production. Bachy Sorgho and India varieties showed the best performance on grain yield compared to Jumbo Super and Pacific Seed 99 varieties. Thus, Brachy Sorgho and India varieties can be recommended for future grain production in mineral soil.

MyPlate Food Model and Family Grocery Shopping with Second and Third Graders are Significant Interventions for the Development of Interactive Health Literacy Skills

A school-based nutrition education intervention was implemented with second and third graders who used their functional health literacy skills of reading, writing, and speaking to learn about the MyPlate food model from the United States Department of Agriculture. The pretest-posttest intervention determined if children had ever seen the MyPlate food picture and if children participated in grocery shopping and meal making events with their family at home. Interactive health literacy accounted for the conversations that children had with their teachers about food and nutrition when learning about the MyPlate food model and when preparing three recipes over three days in the school classroom. Interactive health literacy strategies also included children’s ability to interact and learn about the MyPlate food model as a valid and reliable print and electronic material associated with meal planning. Implications and guidelines for teachers and parents are discussed for advancing the need for and understanding of functional and interactive health literacy for elementary children in the United States.

Predictors Of Handgrip Strength And Its Relationship To Physical Performance In Older Irish Adults

Abstract Background Handgrip strength declines with age and predicts poor physical performance and increased risk of falls and mortality. However, the strength of the relationship between handgrip strength and other physical measures is less well studied. We aimed to explore the predictors of handgrip strength in older Irish adults and its relationship to the Timed and Up and Go (TUG). Methods Study participants were from a longitudinal cohort of Irish adults aged >60 years; Trinity Ulster University Department of Agriculture (TUDA) study. Participants on current or long-term steroids, a history of stroke and with MMSE <24 were excluded. Handgrip strength was assessed with a dynamometer and defined as <27 kg for males and <16 kg for females. Abnormal performance in the TUG was defined as >12 seconds. Predictors of impaired handgrip were explored in multivariate logistic regression. Results There were 748 participants (68.9% female, mean age 70.2 years), of whom 33.4% had abnormal handgrip strength, which was more common in female’s vs males (39.4 vs 20.2%, p<0.001). Both abnormal handgrip strength and TUG was present in 13.6%. Normal handgrip strength had a negative predictive value of 76.0% for ruling out poor TUG, while abnormal handgrip had a positive predictive value of 62% for poor TUG. Predictors of impaired handgrip strength were older age (P<0.0001), female sex (p<0.0001), lower protein intake (P=0.024) and higher waist hip ratio (p=0.014). Conclusion Handgrip strength had a modest correlation with TUG A significant proportion had abnormal TUG but normal handgrip. Changes in lower limb muscle strength (affecting TUG) precede muscle loss in the upper limb. Higher protein intake predicted greater grip strength and supports the recommendation to maintain adequate intakes to prevent sarcopenia. Greater waist hip ratio is associated with reduced lean muscle mass which may explain the negative relationship we identified with handgrip strength.

Predictors Of Loneliness In Older Irish Adults And Its Relationship To Depression

Abstract Background Loneliness can affect up to 30% of older adults and is associated with increased morbidity and all cause mortality. Loneliness is also a major risk factor for depression. We aimed to explore the predictors of loneliness in older Irish adults and its relationship to depression. Methods Study participants were Irish adults aged >60 years in the Trinity Ulster University Department of Agriculture (TUDA) study. Participants with an MMSE <24 were excluded, to prevent the inclusion of individuals with dementia. Loneliness in the last week was identified from the 'loneliness' question in the Center for Epidemiological Studies Depression Scale (CES-D) and considered present when experienced ≥1-2 days in the last week and absent when experienced rarely or none of the time. Depression was defined as a CES-D ≥16. Predictors of loneliness were explored in multinomial regression models as well as its association with depression. Results There were 4561 participants, 70% female, mean age 70.1 years and 21.6% experienced loneliness. Positive independent predictors of loneliness were living alone (OR 5.2, CI 4.41-6.14, p<0.001), female sex (OR 1.29, CI 1.07-1.56, p=0.009), timed up and go >12 seconds (OR 1.46-2.32, p<0.001) non-driving status (OR 1.37, CI 1.13-1.67, P=0.001) and lower MMSE (OR 0.57, CI 0.41-0.79, p=0.0006). Those who reported loneliness had a higher prevalence of depression (32.1 vs 3.3%, p<0.0001). Conclusion Living alone was, as expected, the greatest predictor of loneliness followed by physical frailty, non-driving status, female sex, and lower cognition. A high proportion (one third) who felt lonely had depression, though low mood may increase the perception of loneliness independent of social engagement. A limitation is that we did not have a specific validated tool to assess loneliness or to distinguish between `emotional' or `social' loneliness.

Exploring The Relationship Between SSRIs, Falls, Fractures, And Bone Mineral Density In Older Irish Adults: Results From The TUDA Study

Abstract Background Selective Serotonin Reuptake Inhibitors (SSRIs) are associated with an increased risk of falls and fractures, though the results from studies are inconsistent. SSRIs may cause a decline in bone mineral density (BMD), but this remains unclear. We aimed to explore the relationship between SSRI use and falls, fractures and BMD in older Irish adults. Methods Study participants were from a cross-sectional study of adults aged >60 years; Trinity Ulster University Department of Agriculture (TUDA) study. Falls and falls resulting in fracture in the preceding year were self-reported so participants with an MMSE < 24 were excluded. The relationship between SSRIs and falls and falls with fractures was explored in multivariate logistic regression adjusting for relevant covariates and medications: loop and thiazide diuretics, alpha and beta blockers, calcium channel blockers, ACE-inhibitors, benzodiazepines, opioids. In a subgroup with no prior diagnosis of osteoporosis or on medications for same, the relationship with SSRI's and BMD (as assessed with DXA) was explored. Results There were 4521 participants, 67.6% female, mean age 73.3 ± 8.1 years, 9.4% (n=427) using SSRIs. SSRI use predicted an increased risk of falls in the last year both before and after multivariate adjustment (OR 1.71, 1.37-2.14, p<0.001). In participants with falls (n=1448), those who had a fall with fracture versus a fall with no fracture were no more likely to be using SSRIs. In a subsample, (n=2178) no relationship was found between SSRI use and BMD at the hip, neck of femur or spine. Conclusion SSRIs use was associated with a 71% increased risk of falls though did not predict BMD or fractures. However, duration of SSRI use was not known and there was no prospective follow up. It remains unclear whether increased fracture risk in SSRI users found elsewhere is explained solely by falls risk.

Angiotensin Receptor Blockers, But Not Angiotensin Converting Enzyme Inhibitors, Improve Bone Mineral Density: Results From The TUDA study

Abstract Background Both hypertension and osteoporosis are common in older adults and frequently co-exist. The renin angiotensin system has been implicated in osteoclast activation and bone loss. Several studies have previously examined the relationship between various inhibitors of RAS and bone mineral density (BMD). In this study we examined the relationship between angiotensin receptor blockers (ARBs) and angiotensin converting enzyme ACE inhibitors and BMD. Methods Study participants were from a cross-sectional study of adults aged >60 years; Trinity Ulster University Department of Agriculture (TUDA) study. We excluded participants on treatments for osteoporosis. In users and non-users of ARBs and ACE inhibitors, we compared least squares means BMD measured by densitometry at the neck of femur, hip, and lumbar spine adjusted for age, sex, body mass index, timed up and go, vitamin D, parathyroid hormone, eGFR, diabetes mellitus, smoking, alcohol consumption, dairy intake, glucocorticoid exposure, thiazide, and loop diuretic use. Results There were 2218 participants (mean age 70.1±6.5 years, 58.3% female, 23.4% (n=519) on ARBs and 30.9% (n=686) on ACE inhibitors). In both univariate and multivariate analysis, ARB users had higher BMD than non-users were associated with higher BMD at the neck of femur (0.860 g/cm² vs. 0.846 g/cm², p=0.016); at the total hip, (0.938 g/cm² vs. 0.919 g/cm², p=0.004); at the lumbar spine, BMD was 1.106 g/cm² vs. 1.074 g/cm² p< 0.001). No significant relationship between ACE inhibitors and BMD at any site was observed after multivariate adjustment. Conclusion ARBs were associated with improved BMD while ACE inhibitors were not. Similar results have been found in other studies but the mechanisms but there is substantial uncertainty regarding the mechanisms underlying these relationships. These findings could be important in the choice of antihypertensive agent in older patients at risk of fracture and further research is warranted.

Vitamin D Deficiency Is Associated With An Increased Risk of Diabetes In Older Irish Adults: Results From The TUDA Study

Abstract Background Several studies have shown that vitamin D deficiency may increase the risk of developing type II diabetes and prediabetes. However, the data are inconsistent. Vitamin D may improve insulin sensitivity and pancreatic beta cell function, which could influence diabetes risk. We aimed to examine the relationship between vitamin D deficiency and type II diabetes in older Irish adults. Methods Study participants were from a cross-sectional study of adults aged > 60 years; Trinity Ulster University Department of Agriculture (TUDA) study. Diabetes was defined by self-report, use of diabetic medications or HBA1c ≥ 6.5%. Vitamin D deficiency was defined as 25(OH)D < 30 nmol/l. The relationship between vitamin D deficiency and diabetes was explored in multivariate logistic regression adjusting for age, sex, BMI, waist hip ratio, season, vitamin D supplement use, timed up and go, alcohol intake, current or previous steroid use, smoking, use of statins and socioeconomic status. Results There were 5171 participants (67.2% female, mean age 74.0 years) of whom 19.7% were vitamin D deficient and 15.7% had diabetes. Vitamin D deficiency versus non-deficiency was associated with an increased risk of diabetes both before and after multivariate adjustment (OR 1.46, 1.98-1.79, p<0.0002). Conclusion Vitamin D deficiency was associated with a 46% increased risk of having type II diabetes. However, the results of longitudinal studies and randomised trials have produced inconsistent results, though many had participants with higher baseline 25(OH)D status. A recent review of 46 trials of vitamin D supplementation found that it may reduce Hba1c in diabetics with vitamin D deficiency. Well designed trials to assess the potential effect of vitamin D supplementation on diabetic risk should be conducted.

Geospatial Analysis of Abiotic and Biotic Conditions Associated with Leptospirosis in the Klaten Regency, Central Java, Indonesia.

The Klaten Regency, Central Java Province, Indonesia, is a leptospirosis endemic area. The purpose of this study is to spatially describe the abiotic and biotic environmental factors that contributed to the incidence of leptospirosis in the Klaten Regency in 2018. This was a descriptive observational with a cross-sectional approach conducted in the Klaten Regency, Central Java, in 2019 with 59 respondents. The results revealed that the percentage of abiotic environmental factors such as poor waste disposal facilities, poor gutter conditions, rivers < 200 m, and flooding history, namely 35.6%, 41.2%, 54.2%, and 6.8%, respectively. The highest leptospirosis cases occurred in April 2018, with 325 mm of rainfall, an average temperature of 27 °C, an average humidity of 82.3%, and an altitude of 100-200 MASL (79.7%). Meanwhile, biotic factors included rat nest existence (100%), having pets at risk (32.2%), and ≥three types of vegetation (79.7%). The main result confirmed that all leptospirosis cases had rat nests throughout the respondent's house. This finding emphasizes the importance of rat pest control programs by establishing cross-sectoral collaboration with the Department of Agriculture and educating the public to also play a role in environmental cleanliness in controlling rats.

Abstract B166: Impact of rurality on early-onset colorectal cancer outcomes: Age, geographic disparities, and the need for improved access and awareness in the United States

Abstract BACKGROUND: While overall colorectal cancer (CRC) rates have stabilized or declined in the United States (U.S.), early-onset CRC (EO-CRC), diagnoses before age 50, has increased. Rural U.S. residents face a higher CRC incidence (43.9 vs. 40.1 per 100,000) than urban residents. CRC mortality, once highest in the Northeast, is now most prevalent in the South and Midwest, particularly among Non-Hispanic Black populations. This geographic shift is also evident in EO-CRC. Existing literature lacks comprehensive analysis on the influence of rurality and age at CRC diagnosis. In this study, we sought to elucidate the relationships between age at diagnosis with CRC outcomes, while examining the moderating role of rurality. Methods: We used data from the 2006-2020 Surveillance, Epidemiology, and End Results (SEER) Program. Rurality was defined using census tract level U.S. Department of Agriculture (USDA)’s 2010 Rural Urban Commuting Area (RUCA) codes: rural (codes 8, 9, 10) and urban (all other listed codes). We conducted multilevel regression modeling (logistic and Cox proportional hazards) to examine the associations between age at diagnosis, rurality, and CRC outcomes (late-stage diagnosis and survival), adjusting for race, marital status, sex, and year of diagnosis. Results: We identified 835,907 patients aged 20-79 from the 2006-2020 SEER program. Most patients lived in urban areas (86%), were non-Hispanic white (66%), and married (41%). After adjusting for marital status, race, sex, and diagnosis year, we found that patients aged 30-39 (aOR: 1.49, 95% CI: 1.44–1.55) and 40-49 (aOR: 1.43, 95% CI: 1.39–1.46) had over a 40% increased likelihood of late-stage CRC compared to patients aged 50-59. Urban patients aged 30-39 had a 52% higher likelihood of late-stage CRC (aOR: 1.52, 95% CI: 1.46–1.58). Patients aged 20-29 (aOR: 0.81, 95% CI: 0.77–0.86) had a reduced risk of CRC death compared to those aged 50-59. Conversely, patients aged 40-49 (aOR: 1.09, 95% CI: 1.07–1.11), 60-69 (aOR: 1.16, 95% CI: 1.14–1.17), and 70-79 (aOR: 1.39, 95% CI: 1.28–1.36) had increased risks of CRC death compared to patients aged 50-59. Rural patients aged 20-29 (aOR: 0.79, 95% CI: 0.68–0.94) and 30-39 (aOR: 0.95, 95% CI: 0.87–1.03) had a reduced risk of CRC death compared to rural patients aged 50-59. Conclusion: We observed that patients aged 30-49 were more likely to be diagnosed with late- stage CRC compared to those aged 50-59, particularly in urban areas. To reduce the burden of EO-CRC and prevent advanced-stage diagnoses in young adults, it is essential to enhance access to care and raise awareness of CRC symptoms among younger Americans. Future research should explore urban -rural barriers to healthcare access, including the role of healthcare infrastructure, transportation, and provider availability, to develop strategies for improving EO-CRC early detection and treatment outcomes. Citation Format: Meng-Han Tsai, Justin X. Moore, Caitlyn Grunert. Impact of rurality on early-onset colorectal cancer outcomes: Age, geographic disparities, and the need for improved access and awareness in the United States [abstract]. In: Proceedings of the 17th AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2024 Sep 21-24; Los Angeles, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2024;33(9 Suppl):Abstract nr B166.

PSIX-30 Interaction between rumen microbiome and host feed efficiency phenotype across contrasting breeds and dietary sources

Abstract Provision of feed accounts for a high proportion of the variable cost in beef production systems, and consequently is a major determinant of overall profitability. Thus, improving the conversion of feed to animal product by identifying and breeding cattle with improved feed efficiency potential provides a method through which feed input costs may be reduced, whilst also contributing to more environmentally sustainable beef production. The rumen microbiome dictates the feed degradation capacity and subsequent nutrient supply in ruminants and is thus potentially impacted by feed efficiency phenotype. However, the relationship between rumen microbiota and host feed efficiency phenotype is yet to be fully elucidated. This is further complicated when contrasting diets, cattle breeds as well as various stages of animal development are considered. The objective of this study was to undertake network analysis on rumen microbiome data generated from two contrasting steer breed types (Charolais and Holstein-Friesian), divergent within-breed for residual feed intake (RFI), and offered contrasting diets during different stages of development: i) high-concentrate during the growing phase; ii) grass silage during the growing phase; iii) zero-grazed grass during the growing phase, and iv) high-concentrate during the finishing phase. From approximately 10 mo of age, Charolais (n = 90) and Holstein-Friesian (n = 77) steers were individually fed each of the aforementioned diets for 70 d, following an adaptation period between each diet. At the end of each dietary phase, RFI was determined for every steer and rumen fluid was sampled using a transesophageal sampling device from the 10 most-efficient (Low-RFI) and the 10 least-efficient (High-RFI) steers within each breed. Rumen fluid samples were then subjected to 16S rRNA sequencing and a network-based systems biology analysis subsequently undertaken using PCIT incorporating the 16S sequencing data and individual RFI values across each breed and dietary phase. A total of 110 microbial taxa were connected (P &amp;lt; 0.05; r &amp;gt; 0.8) to the breed and diet contrasts examined; however, no single microbe was commonly connected to all contrasts. The Pyramidobacter genus was the most represented phylotype, displaying a positive connection with the Charolais steers during the grass silage diet, as well as a negative association with Charolais steers during the first high-concentrate diet and Holstein-Friesian steers during the zero-grazed grass diet. Similarly, of the 35 microbial taxa significantly connected to more than one breed/diet contrast, 12 displayed altered direction of association, indicating a differential relationship between the rumen microbiome and RFI phenotype depending on the prevailing dietary management. Acknowledgement: The animal model used in this study was funded by the Irish Department of Agriculture, Food and the Marine (RSF13/S/519). Kate Keogh received funding for this work from the Research Leaders 2025 program (co-funded by Teagasc and the European Union’s Horizon 2020, Marie Skłodowska-Curie grant agreement number 754380).

Characterization of Genetic Diversity in the Capsid Protein Gene of Grapevine Fleck Virus and Development of a New Real-Time RT-PCR Assay.

The grapevine fleck virus (GFkV) is a ubiquitous grapevine-infecting virus found worldwide, is associated with the grapevine fleck complex, and is often found in mixed infections with viruses of the grapevine leafroll complex and/or vitiviruses. Although GFkV has been studied for a long time, limited sequence information is available in the public databases. In this study, the GFkV sequence data available in GenBank and data generated at the Foundation Plant Services, University of California, Davis, were used to perform nucleotide sequence comparisons, construct a phylogenetic tree, and develop a new RT-qPCR assay. Sequence comparisons showed high genetic diversity among the GFkV isolates, and the phylogenetic analyses revealed a new group comprised of GFkV isolates identified in the present study. A new assay, referred to as GFkV-CP, was designed and validated using an existing GFkV positive control together with 11 samples known to be infected with combinations of different marafiviruses and maculaviruses but not GFkV. In addition, the newly designed assay was used in a field survey to screen grapevines from diverse geographical locations that are maintained at the United States Department of Agriculture (USDA) National Clonal Germplasm Repository (NCGR) in Winters, CA.

Classical biological control of the western grape leaf skeletonizer in California, a review 1941–2022

ABSTRACT Western grape leaf skeletonizer was first reported in southern California in 1941 and soon became a serious pest of commercially produced grapes in that region. Their gregarious larvae can easily defoliate vineyards and they remained a major problem for production of grapes until this pest was reunited with its host-specific natural enemies. Efforts to import into southern California effective natural enemies during the 1950s from southern Arizona succeeded in reducing populations of this moth to economically tolerable levels. We report for the first time on releases of natural enemies into central and northern California for control of western grape leaf skeletonizer. Over 600,000 parasitoids new to this region were released during late 1970s–1980s by the state’s Biological Control Program. Foreign exploration by the California Department of Food and Agriculture (CDFA) into Mexico in 1978 resulted in additional parasitoids new to California: a new biotype of Ametadoria harrisinae and a new species of Chetogena sp. (Tachinidae). The high numbers of tachinids released throughout California is credited with aiding in the spread of a highly virulent strain of granulosis virus which in turn caused high levels of mortality to western grape leaf skeletonizer larvae. Reports of WGLS outbreaks in vineyards over the last 15 years are rare, in contrast to previous years.