The name Enterobacter gergoviae sp. nov. is proposed for a group of organisms isolated from various clinical sources in France, Africa, and the United States and from environmental sources. E. gergoviae deoxyribonucleic acid contains 60% guanine plus cytosine. Deoxyribonucleic acid relatedness studies showed E. gergoviae strains to be very highly related, as is characteristic of strains of a single species. The biochemical profiles of E. gergoviae strains were extremely similar. Phenotypically, E. gergoviae belongs to the family Enterobacteriaceae and is most similar to Enterobacter aerogenes. E. gergoviae is distinguishable from E. aerogenes by its positive urea reaction and negative reactions for KCN, sorbitol, mucate, and gelatinase. Strain CIP 76.01 (= ATCC 33028 = CDC 604-77) is the type strain of this new species.