The present study was designed to understand the impact of season on biochemical, metabolic and hormonal parameters in Deoni cow. Study period comprised winter, spring and summer seasons. Ambient temperature and relative humidity were recorded from sensor based automatic weather station and temperature relative humidity index was calculated. Blood samples were collected two times in each season in selected animals. Biochemical parameters viz., glucose, total protein, albumin, triglycerides, cholesterol, creatinine, BUN, Sodium and potassium were estimated using semi-automated biochemistry analyzer. The hormonal parameters viz., tri-iodothyronine, thyroxin and cortisol were estimated by radioimmune assay technique. Glucose, protein, triglycerides, cholesterol and creatinine was significantly lower in summer season. Significantly higher BUN, Na+ and K+ were noticed in summer. T3 and T4 were significantly higher in winter and lowest in summer. The cortisol was significantly higher in summer followed by winter and the lowest was recorded during spring.