Denture Base Research Articles

Three-dimensional accuracy evaluation of post-curing of artificial teeth, denture bases, and denture base monoblocks manufactured by digital light processing

Three-dimensional accuracy evaluation of post-curing of artificial teeth, denture bases, and denture base monoblocks manufactured by digital light processing

Evaluation of the Effect of Impregnation Methods on Flexural Strength of Polypropylene Fibers Reinforced Polymethyl Methacrylate Denture Base Material: An In Vitro Study

Evaluation of the Effect of Impregnation Methods on Flexural Strength of Polypropylene Fibers Reinforced Polymethyl Methacrylate Denture Base Material: An In Vitro Study

COMPARATIVE EVALUATION OF HARDNESS AND SURFACE ROUGHNESS AFTER DIFFERENT COOLING PROCEDURES OF HEAT CURE DENTURE BASE MATERIALS

Aim: To evaluate the influence of different cooling procedures on mechanical properties of DPI & LUCITONE heat cure poly methyl methacrylate denturebase materials before andafter 1year of brushing simulation. Materials And Methods: A total of 100 specimens were prepared using two heat cure denture base materials i.e, DPI and LUCITONE. The specimens in each group were subdivided into five groups (n=10 ) based on the cooling procedure followed: A: Flask remains in water bath till room temperature B: Remove flask from water bath, bench cool for 30min and then cool under running water for 15min C: Remove flask from water bath, bench cool for 10min and then cool under running water for 15min D: Remove flask from water bath and bench cool till room temperature E: Remove the flask from water bath and cool under running water for 15 minutes.After finishing and polishing, samples were placed in a brushing machine to simulate one year of mechanical cleansing. The specimens were tested for surface roughness with profilometer and hardness with Vickers hardness tester before and after brushing. Results were statistically analysed by two and one- way Analysis of variance (ANOVA) plus Tukey post hoc tests. Results: Specimens with type C(Remove flask from water bath, bench cool for 10min and then cool under running water for 15min)cooling procedures have high hardness values, among whichLucitone showed better properties. Surface roughness did not show significant changes among different groups. Brushing did not significantly affect surface roughness and hardness of denture base materials. Conclusion: Two of the treatments tested, Treatment C: bench-cooling for 10 min and cooling under running water for 15min and Treatment A: cooling in water-bath till room temperature provided the highest hardness values.

Effect of Denture Base Material on the Peri-implant Stresses of Implant Supported Overdenture: An In Vitro Study

Effect of Denture Base Material on the Peri-implant Stresses of Implant Supported Overdenture: An In Vitro Study

POLYAMIDE FLEXIBLE DENTURES – A BETTER OR WORSE STRATEGY

Background: Polyamide dentures have added advantages over the acrylic dentures in terms of esthetics, comfort and versatility to adapt to the undercut areas. However, the effect of flexibility of these dentures from the view of residual ridge resorption has always been obscure. The aim of this study is to compare the type of denture base with the distribution of masticatory forces by examining the changes on the alveolar mucosa in the histological picture before and after insertion of two widely used dentures. Materials and methods: A total of 20 participants were included in the study, among which 10 patients were allocated for acrylic removable partial dentures and 10 patients were allocated for polyamide flexible removable partial dentures. The patients were asked to rinse their mouth and cement spatula was used to collect the epithelial mucous cells noninvasively by scraping the crest of the residual alveolar ridge with light pressure in premolar-molar area both before insertion and 2 weeks after insertion of the removable partial dentures. The said swab was then smeared on a clean glass slide and fixed in 95% ethyl alcohol and was stained by Papanicolaou (PAP) stain. The PAP-stained smear was analyzed for the presence of keratinized cells and the number of keratinocytes was counted for both acrylic and polyamide denture groups. Results: The results show that both types of denture bases altered the alveolar mucosal keratinization but there was a significant difference in number of keratinized cells between all the three groups. Polyamide flexible partial dentures showed more ortho-keratinized epithelium, which favours better occlusal load distribution. Conclusion: The results concluded that there was decreased masticatory load on the residual alveolar ridge under polyamide denture group due to the flexibility of the denture base material compared to that of the acrylic denture group.

Dimensional reliability in CAD/CAM production of complete denture bases: A comparative study of milling and various 3D printing technologies.

This study aims to assess the dimensional accuracy of complete denture bases fabricated from different CAD/CAM technologies and a conventional method, including milling (CNC), PolyJet (PJ), laser sintering (SLS), digital light processing (DLP), and injection molding (IM). It also examines the influence of the removal of technology-specific connectors or support structures when present. Denture base surfaces were digitized using a laboratory scanner, and virtual measurement points were calculated with tetrahedral reference geometries. Defined distances were measured in all spatial directions and compared to design data (p<0.05), revealing significant differences in sagittal (p=0.004), transversal (p<0.001), and vertical (p<0.001) dimensions. Connector removal had no significant impact for CNC but significantly affected DLP. All technologies yielded clinically acceptable results, with CNC milling demonstrating the best overall outcome.

Effect of aging on dimensional accuracy and color stability of CAD-CAM milled and 3D-printed denture base resins: a comparative in-vitro study

BackgroundThere is a lack of studies comparing the dimensional accuracy and color stability of denture base resins made using computer-aided design and computer-aided manufacturing (CAD-CAM) milling, 3-dimensional (3D) printing, and conventional denture processing techniques. This makes it challenging to determine the best method to fabricate complete dentures. The objective of this in vitro investigation was to assess and contrast the color stability and dimensional accuracy of denture base resins that were 3D printed and CAD-CAM milled, both before and after aging by thermocycling using digital surface matching technology and a benchtop laser scanner without using a spray, to optimize adaptation of the denture base and cast to minimize any imperfections and to evaluate the impact of the denture cleansing solution on the stability of color.MethodsEvaluation of the dimensional accuracy (n = 27) was completed on a sectional maxillary stone cast using a digital 3D-surface matching software before and after 5000 thermocycles. A spectrophotometer was used to measure the color change (△E00) of all disc specimens (N = 54) before and after 500 thermocycles and immersion in denture cleansing solution for 30 cycles (3 min each) daily for 6 days. The Kruskal Wallis test, Dunn’s post hoc test, Tukey’s test with Bonferroni adjustment, one sample t test and independent t test were used to statistically analyze the data (α < 0.05).ResultsThermocycling decreased the dimensional accuracy of the heat polymerized group at all 5 locations and the 3D-printed group at locations 1, 3 and 5 (P > .05), while it had no significant difference on the CAD-CAM milled group at all locations (P < .05). The color change (△E00) was lowest in the CAD-CAM milled group, moderate in the heat-polymerized group and highest in the 3D-printed group. After immersion in denture cleanser, the color change (△E00) was significantly higher in the 3 groups compared with after thermocycling (P > .001).ConclusionsCAD-CAM milled resins had the highest dimensional accuracy and the best color stability, conventional heat polymerized acrylic resins showed moderate change in dimensional accuracy and color stability, while the 3D- printed resin had the lowest dimensional accuracy and color stability after aging by thermocycling.

Effect of Hybrid Nanofillers on the Mechanical Characteristics of Polymethyl Methacrylate Denture Base Composite

The research study focused on enhancing the mechanical characteristics of polymethyl methacrylate (PMMA) denture bases. PMMA is commonly used in dentistry due to its easy fabrication, cost-effectiveness, and favourable physical properties. However, its limitations include low wear resistance, hardness, and mechanical strength, making it less suitable for long-term dental applications. To address these limitations, the study employed a combination of hybrid nano-fillers, specifically HNTs (halloysite nanotubes) and MWCNTs (multi-walled carbon nanotubes), at varying loading levels to improve the mechanical characteristics of the PMMA composite. These nano-fillers underwent treatment by using a coupling agent to enhance their compatibility with PMMA. Key findings of the research include that introducing HNTs/MWCNTs into the PMMA matrix led to a substantial increase in flexural strength, with a significant improvement of 109.1 MPa compared to unfilled PMMA. This indicates that the composite material became more resistant to bending or deformation. There was a substantial rise in flexural modulus values, suggesting improved stiffness in the nanocomposite compared to unfilled PMMA. In addition, the tensile strength of the PMMA composite increased by 64.4 MPa with the addition of the hybrid nano-fillers, indicating enhanced resistance to stretching or pulling forces. The study found that the improvement in flexural and tensile strength was dependent on the concentration of MWCNTs. Increasing the MWCNT concentration up to 0.75 wt.% led to improved mechanical properties, but further increases resulted in a reduction in PMMA properties. Although there was a modest improvement in Vickers hardness (approximately 18.93 kg/mm&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;), the addition of HNTs/MWCNTs as hybrid nano-fillers effectively enhanced the properties of the PMMA nanocomposite. The study concludes that incorporating hybrid nano-fillers into PMMA could contribute to the longevity and durability of dental composites, addressing some of the material&amp;apos;s inherent limitations. The specific combination and concentration of nano-fillers played a crucial role in determining the mechanical properties of the resulting nanocomposite.

Effect of Addition of Metal Oxide Nanoparticles on the Strength of Heat-Cured Denture Base Resins: Protocol for Systematic Review and Meta-Analysis of In Vitro Studies.

Metal oxide nanoparticle-reinforced polymethyl methacrylate (PMMA) has been shown to improve mechanical properties, such as strength. Different types of metal oxide nanoparticles have been used previously, but the comparative effect on the strength of heat-cured denture base resins remains unclear. This is a protocol for a systematic review and meta-analysis that will aim to pool evidence to compare and analyze the effects of the addition of different metal oxide nanoparticles, with varied sizes and concentrations, on the strength (flexural, impact, transverse, compressive tensile strength, and fracture toughness) of heat-cured PMMA. In addition, this review aims to analyze methodological factors, such as adherence to testing and sample-making guidelines, and the effects of surface treatments of the nanoparticles on the strength of heat-cured denture base resins. The protocol has been registered in the Open Science Framework. Search strategies to identify studies on the effect of metal oxide nanoparticles on the strength of heat-cured PMMA were developed by the subject matter expert in library science. Following this, a systematic search of 5 electronic databases (PubMed [NCBI], Scopus [Elsevier], Cochrane Library [Wiley], CINAHL Plus with Full Text [EBSCO], and Dimensions Free Web App) was conducted to retrieve in vitro studies published in English from January 2012 to October 2023. Along with this citation chasing, other online sources and gray literature were also searched. Furthermore, papers will be screened, and appropriate data elements will be extracted in a standardized manner. A risk-of-bias assessment will be performed using a modified Cochrane Risk of Bias Tool. A meta-analysis will be performed using a random-effects model. Search in databases resulted in 1837 papers, of which 1752 were duplicates, leaving 85 records that were screened for titles and abstracts based on the eligibility criteria. A similar search run on other online sources identified 129 papers that will be further analyzed for inclusion. The study was initiated in November 2023 and research questions and search strategies were formulated. The proposed study is expected to be completed by December 2024. This systematic review will comprehensively analyze the effects of the incorporation of metal oxide nanoparticles in heat-cured denture base resins on the strength of the material. We anticipate gaining a deeper understanding of the effects and method of use of metal oxide nanoparticles to improve the strength of PMMA denture base resins. PRR1-10.2196/59999.

The Antimicrobial Effect of the Incorporation of Inorganic Substances into Heat-Cured Denture Base Resins—A Systematic Review

The Antimicrobial Effect of the Incorporation of Inorganic Substances into Heat-Cured Denture Base Resins—A Systematic Review

Reinforcement of 3D-printed resins for denture base by adding aramid fibers: Effect on mechanical, surface, and optical properties.

The present study evaluated the mechanical, surface, and optical properties of 3D-printed resins for removable prostheses reinforced by the addition of aramid fibers. According to ISO 20795-1:2013 standards, specimens were printed using a digital light processing 3D printer and divided into two groups (n=06/group): 3D-printed resin for denture base as the control group, and a group with the same 3D-printed resin in addition of 5% aramid fibers as the experimental group. Red aramid fibers were chosen for aesthetic characterization. The specimens were evaluated for their mechanical properties, such as elastic modulus (GPa), flexural strength (MPa), and superficial properties by their surface microhardness (KHN), surface roughness (μm), and surface free energy (mJ/m2). Optical properties were evaluated by the color difference (∆E00) between groups. The statistical test chosen after the exploratory analysis of the data was One-way ANOVA followed by Tukey's HSD (α=0.05). The results showed statistical differences in elastic modulus (p<0.0001), flexural strength (p<0.0001), surface free energy polar variable (p=0.0322), total surface free energy (p=0.0344), with higher values for the experimental. Surface hardness and surface roughness showed no statistical difference (p≥0.05). The color difference (∆E00) obtained through the CIEDE2000 calculus was below the perceptibility threshold (≤1.1). Adding aramid fibers to 3D-printed resin for denture bases resulted in better mechanical properties, without major alterations in surface properties. In addition, it is an easy-to-apply choice for mechanical reinforcement and aesthetic characterization, with the expression of small blood vessels in the 3D-printed resin for removable denture bases.

Flexural strength, fracture toughness, translucency, stain resistance, and water sorption of 3D-printed, milled, and conventional denture base materials.

To compare mechanical, optical, and physical properties of denture base materials fabricated with various 3D printing systems to reference milled and conventionally heat-processed denture base materials. Specimens of denture base materials were either 3D-printed (DLP in-office printer, CLIP laboratory printer, or material jetting laboratory printer), milled, or heat processed. 3-point bend flexural strength testing was performed after 50 hours of water storage following 1hour of drying (dry testing) or in 37°C water (wet testing). Fracture toughness was performed with a notched beam specimen after 7 days of water storage and tested dry. The translucency parameter was measured with 2mm thick specimens. Stain resistance was measured as color change following 14 days of storage in 37°C coffee. Water sorption was measured following 7 days of storage in 37°C distilled water. For dry testing, all but one of the 3D-printed materials attained higher or equivalent flexural strength as the reference materials. For wet testing, all 3D-printed materials attained higher or equivalent strength as the reference materials and dry-tested materials. For 3D-printed materials, wet testing increased displacement before fracture whereas it decreased displacement for the reference materials. Only two of the 3D-printed materials had similar fracture toughness as the reference materials. One of the 3D-printed materials was more translucent and one was more opaque than the reference materials. Only one of the 3D-printed materials absorbed more water than the reference materials. 3D-printed denture base materials have mostly equivalent mechanical, optical, and physical properties to conventional and milled denture base materials.

Evaluation of the Bond Strength of Acrylic Teeth to Denture Base after Various Chemical Surface Treatments: An In Vitro Study.

The purpose of the present study was to assess the bonding capacity and efficacy of acrylic teeth to denture bases following two different chemical surface treatments. A two-metal mold measuring 35 mm in length and 12 mm in diameter was created specifically for the investigation in order to standardize the wax pattern-based tooth attachment at 45°. Following standard protocol, 75 wax cylinder specimens were flasked, dewaxed, and surface treatment of teeth was done as follows with 25 samples in each group-group I: control group, group II: monomethyl methacrylate monomer group, group III: acetone group. The curing process was completed following the packing of the denture base material. The samples' shear bond strength was assessed using a universal testing machine. Every sample was taken out when it fractured, and the shear load (Newton, N) was noted. The significance of the variation in applied shear load was assessed using one-way analysis of variance (ANOVA) and post hoc ANOVA Tukey's honestly significant difference (HSD) test at the 5% level of significance. The maximum shear bond strength was found in the samples treated with acetone (183.21 ± 0.06) followed by samples treated with monomethyl methacrylate monomer (171.64 ± 0.12) and the control group (149.32 ± 0.04). A statistically significant difference was found between the different groups (p < 0.001). In conclusion, according to the current study's findings, acetone chemical surface treatment of acrylic teeth produced the strongest bond when compared with the control group and monomethyl methacrylate monomer. In prosthodontic practice, artificial teeth regularly de-bond and separate from the denture base. A weak interface is produced when certain clinical conditions, such as ridge prominence, cause excessive cutting of the acrylic teeth and base. Where the denture base polymer meets the teeth's highly cross-linked matrix, it de-bonds adhesively. Therefore, the bonding between the acrylic teeth and the denture base material can be improved by the chemical surface treatment. How to cite this article: Chaudhuri NG, Lahiri B, Francis NT, et al. Evaluation of the Bond Strength of Acrylic Teeth to Denture Base after Various Chemical Surface Treatments: An In Vitro Study. J Contemp Dent Pract 2024;25(6):514-517.

Comparison of Direct Intraoral Scan and Traditional Impression for CAD/CAM Mandibular Overdenture Base: RCT on Peri-implant Marginal Bone Changes.

This study aimed to evaluate the impact of digital vs traditional impression techniques on peri-implant vertical bone resorption in the creation of mandibular overdenture bases supported by four implants using CAD/CAM technology. Twenty edentulous patients were placed in four mandibular implants and randomly divided into groups: (A) the control group (CIG) (n = 10); patients obtained CAD/CAM denture base using conventional impression technique and group (B) the study (DIG) group (n = 10); patients obtained CAD/CAM denture base using digital impression technique. Peri-implant vertical bone height was measured immediately (T0), 6 (T6), and 12 (T12) months after insertion. Peri-implant vertical bone loss (VBL) was calculated first 6 months (T1), the second 6 months (T2), and 1 year (T3) after insertion. For both groups, the survival rates of inserted implants were 100%. The amount of VBL in the first year in both groups was within normal ranges. In both groups, VBL significantly decreased over time. The control group recorded significantly higher VBL than (DIG) group at T2 (p = 0.006) and at T3 (p = 0.005). Digital intraoral scanning technique may be considered a more beneficial registration method than traditional impression technique for the construction of CAD/CAM 4-implant-assisted overdenture base regarding the preservation of vertical bone levels. Both digital intraoral scanners and conventional impression techniques can be used for the construction of CAD/CAM-implant-assisted overdenture bases regarding the preservation of peri-implant vertical bone resorption. How to cite this article: Seifeldeen AR, Aboelez MA, Gebreel AA, et al. Comparison of Direct Intraoral Scan and Traditional Impression for CAD/CAM Mandibular Overdenture Base: RCT on Peri-implant Marginal Bone Changes. J Contemp Dent Pract 2024;25(6):527-534.

An approach for accurately orienting artificial teeth into their denture base sockets of CAD‐CAM milled dentures: A dental technique

AbstractA novel technique was devised to create a stabilizing guide to accurately maintain the position of CAD‐CAM‐milled artificial teeth in their denture base socket during the bonding process. This ensures that the artificial teeth and denture base are adequately bonded to reproduce the designed occlusion and reduce chair‐side adjustment.

Four Different Build Angles in 3D-Printed Complete Denture Bases: A Comparative In Vitro Study

In this study, we aimed to investigate the differences in tissue surface adaptation and the variations in distances between reference points on the polished surfaces of 3D-printed denture bases produced at different build angles. The build angles were 0°, 30°, 60°, and 90°, with 15 denture bases printed for each angle. Using the Geomagic Control® software, a 3D best-fit alignment was conducted between the denture base tissue surface and the reference shape of the edentulous maxilla model to calculate the root mean square error. The distances between reference points on the polished surface were measured using digital calipers. A one-way analysis of variance was conducted for statistical analysis. The adaptation, as measured by the root mean square error, varied significantly among denture bases with different build angles. The distances between the anterior and posterior reference points of the polished surface were also significantly different. However, within the limitations of this study, the variations in adaptations and dimensional accuracy across different build angles were within clinically acceptable ranges. In clinical practice, the print angle can be adjusted based on factors such as printing time, resin consumption, and the number of denture bases being printed simultaneously.

The Effect of Incorporating Dimethylaminohexadecyl Methacrylate and/or 2-Methacryloyloxyethyl Phosphorylcholine on Flexural Strength and Surface Hardness of Heat Polymerized and 3D-Printed Denture Base Materials.

A major disadvantage of polymethyl methacrylate (PMMA) acrylic resins is susceptibility to biofilm accumulation. The incorporation of antimicrobial agents is a reliable prevention technique. The purpose of this study is to investigate the effect of incorporating dimethylaminohexadecyl methacrylate (DMAHDM) and/or 2-methacryloyloxyethyl phosphorylcholine (MPC) into heat-polymerized (HP) and 3D-printed (3DP) denture base materials on the flexural strength, modulus of elasticity, and surface hardness. DMAHDM and/or MPC were mixed with the acrylic resin liquid of a heat-polymerized (ProBase Hot) and a 3D printed (NextDent Denture 3D) material at mass fractions of 1.5% and 3% and a combination of 3% MPC and 1.5% DMAHDM. Significant differences in mechanical properties between the control and experimental groups have been detected (p-value < 0.0001). In HP materials, the addition of DMAHDM and/or MPC generally decreased the flexural strength, from (151.18 MPa) in G1 down to (62.67 MPa) in G5, and surface hardness, from (18.05 N/mm2) down to (10.07 N/mm2) in G5. Conversely, in 3DP materials, flexural strength was slightly enhanced, from (58.22 MPa) in G1 up to (62.76 MPa) in G6, although surface hardness was consistently reduced, from (13.57 N/mm2) down to (5.29 N/mm2) in G5. It is recommended to carefully optimize the concentrations of DMAHDM and/or MPC to maintain mechanical integrity.

Reduction of Multispecies Biofilms on an Acrylic Denture Base Model by Antimicrobial Photodynamic Therapy Mediated by Natural Photosensitizers.

The aim of this study is to investigate the antimicrobial efficacy of antimicrobial photodynamic therapy (PDT) using natural photosensitizers (curcumin, riboflavin, and phycocyanin) and light-emitting diode (LED) irradiation against multispecies biofilms in an acrylic denture base model. Forty-five acrylic specimens were fabricated using heat-curing acrylic resin. The specimens were then infected with a mixed culture of bacterial and fungal species (including Streptococcus mutans, Streptococcus sanguinis, Candida albicans, and Candida glabrata) for 4 days. The acrylic discs were divided into nine groups, with each group containing five discs: control, 0.2% chlorhexidine, 5.25% sodium hypochlorite, curcumin, riboflavin, phycocyanin alone or along with LED. After treatment, the number of colony-forming units (CFUs) per milliliter was counted. In addition, the extent of biofilm degradation was assessed using the crystal violet staining method and scanning electron microscopy. All experimental groups exhibited a significant reduction in colony numbers for both bacterial and fungal species compared to the control (p < 0.001). The PDT groups exhibited a statistically significant reduction in colony counts for both bacteria and fungi compared to the photosensitizer-only groups. The results of this in vitro study show that PDT with natural photosensitizers and LED devices can effectively reduce the viability and eradicate the biofilm of microorganisms responsible for causing denture infections.

Evaluating the physical properties of microwave-cured and heat-cured acrylic denture base materials after the addition of ZrO2 nanoparticles.

Background: The structural, physical, and mechanical properties of denture-based acrylic resin are still compromised by water sorption, water solubility, and porosity. These issues have long called for improved techniques, and the use of nanoparticles is one of them. The main objective of the current investigation was to evaluate and contrast some physical parameters (water sorption, water solubility, and porosity) after the incorporation of ZrO2 nanoparticles (0%, 3% and 5%) into heat-cured acrylic denture base materials (Ivoclare, Major) and microwave-cured acrylic (Acron MC). Materials and methods: A total of 60 resin specimens were manufactured, including 5 specimens for each concentration of ZrO2 NP (30 for porosity and 30 for water sorption and solubility). Followed the manufacturer's directions for preparing the sample of each material. Results: The results were analysed with descriptive statistics, Analysis of Variance(ANOVA) test, Duncan multiple range test, and independent T test demonstrated that the addition of nanoparticles (3% and 5%) decreased water sorption, water solubility, and porosity of PMMA(polymethyl methacrylate) for both types(microwave-cured and heat-cured acrylic denture base materials). Where, at 3%, the ZrO2 nanoparticles showed the best values for all tests compared to the control group. Conclusions: ZrO2 (3% and 5%) nanoparticle reinforcement of acrylic resin can be a useful tactic for lowering water sorption, solubility and porosity, thereby enhancing the performance of the material in various applications for microwave-cured and heat-cured acrylic denture base materials.

The wettability of complete denture base materials constructed by conventional versus digital techniques: an in-vitro study

BackgroundDecreased salivary flow can make the patients uncomfortable with their complete dentures and affects the retention of the dentures. Milling and 3D printing have become an alternative to conventional denture construction techniques. The goal of this study was to evaluate the effect of conventional and digital techniques of the complete denture construction on the denture surface wettability with distilled water and saliva substitute before and after thermocycling.MethodsA total of 30 specimens were utilized in the present study. Specimens were divided according to the construction techniques into 3 groups (n = 10 each). Group I: Heat-polymerized polymethylmethacrylate (PMMA) group, group II: Milled group, and group III: 3-dimensional (3D)-printed group. All the specimens were subjected to 2000 cycles of thermal aging in a thermocycler. The wettability of all specimens to water and saliva substitute was measured via a contact angle goniometer (Olympus TGHM, Rame-hart Inc, USA) before and after thermocycling. Descriptive statistical analysis, plots, and the Shapiro-Wilk test were used to verify normality for each variable. One-way ANOVA was used to compare the 3 study groups, while paired samples t-test was used to compare the differences within each group (P < .05).ResultsThe smallest contact angle of drop of water to the denture base specimens before and after thermocycling were recorded in the milled group (53.0 ± 4.77 and 50.27 ± 2.30, respectively), followed by the heat polymerized PMMA group (85.65 ± 4.71 and 65.06 ± 2.27, respectively), and the 3D-printed group (91.34 ± 6.74 and 90.86 ± 8.57, respectively). While the smallest contact angle of drop of saliva substitute to denture base specimens was recorded in the milled group (56.82 ± 2.29 and 34.85 ± 7.51, respectively), followed by the 3D-printed group (72.87 ± 4.83 and 58.14 ± 9.58, respectively) and the heat polymerized PMMA group (83.62 ± 4.12 and 67.82 ± 4.93, respectively). There was statistically significant difference between the groups (P < .05). A significant decline in the average contact angle of drop of saliva has been reported in all groups after thermocycling. The contact angle values differed significantly between saliva substitute and distilled water in both 3D-printed and milled groups after thermocycling (P < .001).ConclusionsThe milled denture base material presented the best wettability to water and saliva substitute than the 3D-printed and the heat-polymerized PMMA materials. Saliva substitutes improve the wetting ability of denture base materials manufactured by CAD/CAM compared with water.