Dental Wear Analyses Research Articles

Paleoenvironment in East Java during the last 25,000 years as inferred from bovid and cervid dental wear analyses

This paper reports the results of dental mesowear and microwear analyses of bovid and cervid specimens recovered from Late Pleistocene to Middle Holocene deposits of Braholo Cave and Song Terus in Eastern Java. The aim is to infer ungulate dietary traits from dental wear signatures and gain insights regarding the paleoenvironment of the region during the last 25,000years, a period of drastic climatic fluctuations. We considered three cervid (Rusa, Axis and Muntiacus muntjak) and two bovid (Bubalus and Bos javanicus) taxa in the analyses. Results from both cave sites suggest that the Pleistocene-Holocene transition was accompanied by expansion of rainforests as evidenced by the predominance of species that had a browsing or browse-dominated mixed diet. However some of the taxa examined exhibited strong grazing signatures indicative of the continued presence of patches of grasslands up to the Mid-Holocene. Dental wear signatures of specimens from layers dating to the Last Glacial Maximum (LGM) suggest a mosaic environment dominated by grasslands. In Braholo Cave, we observed discrepancies between the mesowear and microwear signatures in specimens dating to the LGM and we take this as evidence of seasonality during this period. The results of the study provide important information on the environments encountered by the hunter-gatherer communities that lived in East Java in periods of climatic change.

Dietary adaptability of Late Pleistocene Equus from West Central Mexico

Most of the Pleistocene species of Equus from Mexico have been considered to be grazers and highly specialized on the basis of their craniodental features, and therefore analogous to their modern relatives from an ecological point of view. Here we rely on dental wear analyses of three different species of Rancholabrean Equus that exhibit differences in limb proportions and body mass and occurred in two different habitats in order to test whether species exhibited more heterogeneous dietary capabilities compared to modern horses. Overall, and although our analyses show grazer lifestyles, there is a significant degree of dietary variability among taxa according to the site, apparently as a response to differences in climate and environmental conditions, with intermediate feeding preferences on the basis of the last items consumed during the last days prior to death in some cases. Accordingly, fossil Equus might have been more sensitive to different environments and diverse in terms of dietary preferences, thus incorporating a higher proportion of browse vegetation if necessary, than their modern relatives. These results highlight the dietary plasticity of certain fossil species, and evidence the caution that should be paid when reconstructing the ecology of fossil species using their modern relatives as analogs.

Investigation of equid paleodiet from Schöningen 13 II-4 through dental wear and isotopic analyses: Archaeological implications

The paleodietary traits of the equid population from Schöningen 13 II-4 were investigated through tooth mesowear and microwear analyses, as well as stable isotopic analyses. The mesowear pattern observed on the upper teeth indicates a low abrasion diet with a significant amount of browse in the diet of the horses. The tooth microwear analysis and the isotopic data confirm that the horses from Schöningen 13 II-4 were mixed feeders, like many populations from other Pleistocene localities in Northern and Eastern Europe. Microwear also provides information on seasonal changes in the diet of the horses and offers the possibility to test hypotheses about the presence of one or several horse populations. Our analysis determined that the assemblage of horse remains from Schöningen 13 II-4 resulted from multiple accumulation events, which took place at different periods of time.

Extending dental mesowear analyses to Australian marsupials, with applications to six Plio-Pleistocene kangaroos from southeast Queensland

Mesowear analysis is a form of dental wear analysis used to infer the diets of herbivorous mammal species. It makes use of percentage indices of blunt, round and sharp cusp shape and high occlusal relief to classify the diet of species into one of three categories: browser, grazer or mixed feeder. Previously, this form of analysis has been limited to placental mammals, restricting the use of such analyses in Australia where the dominant herbivorous mammalian fauna consist of marsupials. In order to address this limitation, mesowear variables of extant marsupials were examined to determine whether their diets can accurately be predicted using mesowear analyses. Discriminant Function Analysis of mesowear variables and analysis of variance (ANOVA) of univariate mesowear scores for marsupial species demonstrate that mesowear analysis can be used to classify marsupial diets. A dataset of 24 typical marsupial species considered to be representative of the three dietary categories with respect to mesowear was generated and significantly increased cross-validated classification levels from 74.4% to 100% for the second molar. Mesowear analysis for marsupial species is most effective for the second molar with high predictive power also being evident for the first and third molars. When mesowear analysis was applied to six Plio-Pleistocene macropods (Marsupialia: Macropodidae) from the Darling Downs region, southeast Queensland, all species were classified as mixed feeders with the exception of Protemnodon roechus which was classified as a grazer. This study demonstrates the effectiveness of mesowear analysis as a dietary proxy for herbivorous marsupial species.

Having the stomach for it: a contribution to Neanderthal diets?

Due to the central position of diet in determining ecology and behaviour, much research has been devoted to uncovering Neanderthal subsistence strategies. This has included indirect studies inferring diet from habitat reconstruction, ethnographic analogy, or faunal assemblages, and direct methods, such as dental wear and isotope analyses. Recently, studies of dental calculus have provided another rich source of dietary evidence, with much potential. One of the most interesting results to come out of calculus analyses so far is the suggestion that Neanderthals may have been eating non-nutritionally valuable plants for medicinal reasons. Here we offer an alternative hypothesis for the occurrence of non-food plants in Neanderthal calculus based on the modern human ethnographic literature: the consumption of herbivore stomach contents.

Opportunistic Feeding Strategy for the Earliest Old World Hypsodont Equids: Evidence from Stable Isotope and Dental Wear Proxies

BackgroundThe equid Hippotherium primigenium, with moderately hypsodont cheek teeth, rapidly dispersed through Eurasia in the early late Miocene. This dispersal of hipparions into the Old World represents a major faunal event during the Neogene. The reasons for this fast dispersal of H. primigenium within Europe are still unclear. Based on its hypsodonty, a high specialization in grazing is assumed although the feeding ecology of the earliest European hipparionines within a pure C3 plant ecosystem remains to be investigated.Methodology/Principal FindingsA multi-proxy approach, combining carbon and oxygen isotopes from enamel as well as dental meso- and microwear analyses of cheek teeth, was used to characterize the diet of the earliest European H. primigenium populations from four early Late Miocene localities in Germany (Eppelsheim, Höwenegg), Switzerland (Charmoille), and France (Soblay). Enamel δ13C values indicate a pure C3 plant diet with small (<1.4‰) seasonal variations for all four H. primigenium populations. Dental wear and carbon isotope compositions are compatible with dietary differences. Except for the Höwenegg hipparionines, dental microwear data indicate a browse-dominated diet. By contrast, the tooth mesowear patterns of all populations range from low to high abrasion suggesting a wide spectrum of food resources.Conclusions/SignificanceCombined dental wear and stable isotope analysis enables refined palaeodietary reconstructions in C3 ecosystems. Different H. primigenium populations in Europe had a large spectrum of feeding habits with a high browsing component. The combination of specialized phenotypes such as hypsodont cheek teeth with a wide spectrum of diet illustrates a new example of the Liem’s paradox. This dietary flexibility associated with the capability to exploit abrasive food such as grasses probably contributed to the rapid dispersal of hipparionines from North America into Eurasia and the fast replacement of the brachydont equid Anchitherium by the hypsodont H. primigenium in Europe.

First evidences of an underwater Final Pleistocene terrestrial extinct faunal bone assemblage from Central Chile (South America): taxonomic and taphonomic analyses

Mesowear and microwear on enamel from 763 teeth of middle and late Pleistocene ungulates were analysed to infer the potential of dental wear analysis of faunal remains as a paleoenvironmental and paleoclimatic proxy in relation to climatic changes and diversity of vegetation available in the environment. Fossil localities including levels belonging to two glacial and two interglacial stages were selected in Germany, France, and Spain. At a temporal scale, results indicate that the dietary diversity in ungulates is higher during interglacial phases (MIS 5 and 3) than during pleniglacial phases (MIS 8 and 4). Dietary diversity is concluded to be related to climate-driven vegetation changes which during interglacials lead to increased variety of potential food items available to ungulates. At the geographical scale, during interglacials, changes in diet composition are evident along geographical gradients. The corresponding dietary gradients are proposed to be related to climate and vegetation gradients reflecting more arid climates in the Mediterranean area compared to North-Western Europe. Species consistently represented at all localities investigated are Cervus elaphus (Cervidae, Artiodactyla) and Equus ferus (Equidae, Perissodactyla). C. elaphus populations are found to consistently have less abrasive diets than E. ferus populations but dietary traits of both species varied largely, revealing a significant plasticity in the feeding adaptation of both species. Those traits are concluded to be related to differences in vegetation structure at each locality and complement the evidence that ungulates have broader dietary habits than what is usually assumed.

The use of gross dental wear in dietary studies of extinct lagomorphs

Studies of paleoenvironments have commonly focused on large mammalian herbivores such as ungulates. Many localities, however, have yielded large numbers of small mammalian herbivores, including lagomorphs and rodents. These fossils represent an untapped paleoecological resource. However, the fossils are often in the form of isolated teeth, and microwear analysis cannot be used due to taphonomic alteration. As a result, we use ungulate gross dental wear as a model. The dental wear features of extant western Canadian lagomorphs are identified and used to create dietary categories that can be applied to make predictions about the diets of extinct forms. The Horse Local Fauna of the Cypress Hills Formation of Saskatchewan has yielded approximately 2,500 fossil specimens, of which nearly 300 are lagomorphs. Two leporid species (rabbits and hares) are present in the Horse Local Fauna, Palaeolagus temnodon and Megalagus brachyodon. Qualitative analysis of the gross dental wear of the lagomorphs of the Horse Local Fauna indicates that M. brachyodon was mainly folivorous and P. temnodon was primarily frugivorous, suggesting that the contemporaneous ecosystem was tree dominated. Gross dental wear analysis allows the use of small herbivores and isolated teeth in paleoecological studies. Studying the diets of small herbivorous mammals will allow more nearly complete reconstructions of past environments and will become increasingly important as more detailed reconstructions are required by paleontologists.

The use of gross dental wear in dietary studies of extinct lagomorphs

Studies of paleoenvironments have commonly focused on large mammalian herbivores such as ungulates. Many localities, however, have yielded large numbers of small mammalian herbivores, including lagomorphs and rodents. These fossils represent an untapped paleoecological resource. However, the fossils are often in the form of isolated teeth, and microwear analysis cannot be used due to taphonomic alteration. As a result, we use ungulate gross dental wear as a model. The dental wear features of extant western Canadian lagomorphs are identified and used to create dietary categories that can be applied to make predictions about the diets of extinct forms. The Horse Local Fauna of the Cypress Hills Formation of Saskatchewan has yielded approximately 2,500 fossil specimens, of which nearly 300 are lagomorphs. Two leporid species (rabbits and hares) are present in the Horse Local Fauna, Palaeolagus temnodon and Megalagus brachyodon. Qualitative analysis of the gross dental wear of the lagomorphs of the Horse Local Fauna indicates that M. brachyodon was mainly folivorous and P. temnodon was primarily frugivorous, suggesting that the contemporaneous ecosystem was tree dominated. Gross dental wear analysis allows the use of small herbivores and isolated teeth in paleoecological studies. Studying the diets of small herbivorous mammals will allow more nearly complete reconstructions of past environments and will become increasingly important as more detailed reconstructions are required by paleontologists.

Palaeoecology of the Mammoth Steppe fauna from the late Pleistocene of the North Sea and Alaska: Separating species preferences from geographic influence in paleoecological dental wear analysis

The paleodietary ecology of Late Pleistocene ungulate faunas of the Mammoth Steppe ecosystem was investigated at Fairbanks (Alaska) and Brown Bank (North Sea) through dental mesowear and microwear analysis. The purpose of the study is to address questions concerning the paleoecology of the Mammoth Steppe, an ecosystem that has no extant analog. Dental wear patterns indicate that the niche partitioning at Brown Bank (BB) region was consistent with ecosystem dynamics found in diverse ungulate faunas in recent time. In contrast, despite the lower numbers of extinct taxa, the Fairbanks (FB) fauna is ecologically bizarre. In general, the microwear of this fauna includes excessive numbers of very (narrow) fine scratches that are atypical for extant mixed feeders and grazers. Moreover, the mesowear signal suggests unusual paleodiets (niche dynamics), where low-crowned mixed-feeding cervids, Rangifer and Cervus, adopted diets that are similar to Equus in terms of mesowear (abrasion) and much more abrasive than the diets of either Bison or Ovis. We suggest that the anomalies may relate to unique, possibly disharmonious, ecosystem dynamics during the Pleistocene–Holocene transition. In addition to these findings, it was discovered (found) that dental wear patterns, particularly microwear variables such as scratch frequency and scratch width are strongly affected by geographic region, irrespective of species-specific dietary preferences.

Late and middle Pleistocene ungulates dietary diversity in Western Europe indicate variations of Neanderthal paleoenvironments through time and space

Mesowear and microwear on enamel from 763 teeth of middle and late Pleistocene ungulates were analysed to infer the potential of dental wear analysis of faunal remains as a paleoenvironmental and paleoclimatic proxy in relation to climatic changes and diversity of vegetation available in the environment. Fossil localities including levels belonging to two glacial and two interglacial stages were selected in Germany, France, and Spain. At a temporal scale, results indicate that the dietary diversity in ungulates is higher during interglacial phases (MIS 5 and 3) than during pleniglacial phases (MIS 8 and 4). Dietary diversity is concluded to be related to climate-driven vegetation changes which during interglacials lead to increased variety of potential food items available to ungulates. At the geographical scale, during interglacials, changes in diet composition are evident along geographical gradients. The corresponding dietary gradients are proposed to be related to climate and vegetation gradients reflecting more arid climates in the Mediterranean area compared to North-Western Europe. Species consistently represented at all localities investigated are Cervus elaphus (Cervidae, Artiodactyla) and Equus ferus (Equidae, Perissodactyla). C. elaphus populations are found to consistently have less abrasive diets than E. ferus populations but dietary traits of both species varied largely, revealing a significant plasticity in the feeding adaptation of both species. Those traits are concluded to be related to differences in vegetation structure at each locality and complement the evidence that ungulates have broader dietary habits than what is usually assumed.

Right handedness of Homo heidelbergensis from Sima de los Huesos (Atapuerca, Spain) 500,000 years ago

Handedness is a product of brain specialization, which in turn seems to be responsible for the higher cognitive capabilities of humans, such as language and technology. Handedness in living humans is well established and shows the highest degree of manual specialization. Studies on hand laterality in nonhuman primates, particularly in chimpanzees, remain a matter of controversy as results tend to vary depending on factors such as the tasks performed and the environment in which the individuals live. Studies in several disciplines have attempted to determine where in the course of human evolution handedness established itself, with evidence collected from sources such as paleoneurological analyses, stone tool flaking, zooarchaeological studies and dental wear analyses, the last one of which have proven the most reliable source of information. Here we report an experimental and paleoanthropological study on hand laterality of a sample of 28 hominids from Sima de los Huesos (Atapuerca, Spain), dated at about 500,000 years ago, and compare our results with dental microwear analysis in other fossil samples such as that from Krapina (Croatia), as well as modern traditional societies. Our results indicate that European Middle Pleistocene Homo heidelbergensis was already as right-handed as modern populations.

A new application of dental wear analyses: estimation of duration of hominid occupations in archaeological localities

Characterization of settlement patterns is one of the core concepts in archeological research. The duration of an occupation is usually estimated through zooarchaeology (e.g., density of remains, cementochronology) and is limited by taphonomic processes and sample size. We propose a new application of dental wear methods for estimating the relative duration of hominid settlements in Paleolithic sites. Dental microwear is known to be sensitive to seasonal changes in diet. In this new application we use microwear scratch counts to estimate the variation in the dietary signal of various ungulate species. We propose that this variation is correlated to the duration of site occupation. Each season presents a limited and different set of food resources available in the environment. If animals are sampled only during a specific season (i.e., during a short term occupation) then they would be expected to have a dental wear signal with little variation. On the other hand, a greater diversity of food is available across different seasons. Therefore, if game animals are hunted through various seasons during long occupation periods, then they would be expected to have more variable dental wear. The application of this technique to the Middle Paleolithic site of Arago Cave (France), where various types of occupations occurred, supports this hypothesis. When combined with multidisciplinary studies of archaeological localities (seasonality in particular), this new application of dental wear analysis presents valuable information about hominid settlements and behavior. We contextualize our data with results from lithic and zooarchaeological analyses from Arago. These results reveal the presence of both high and low mobility groups of Homo heidelbergensis throughout the sequence of the Arago Cave.

Seasonality and intra-site variation of Neanderthal occupations in the Middle Palaeolithic locality of Payre (Ardèche, France) using dental wear analyses

The reconstruction of Palaeolithic subsistence and settlement systems at the Middle Palaeolithic site of Payre (France) is undertaken through the application of dental wear analyses combined to zooarchaeological, technological, and ecological indicators. Three archaeological levels were investigated. Level D, dated to MIS 5, could correspond to an occupation during the cold season. In levels G and F, dated to MIS 8-7, animals were probably hunted during a warmer season. According to dental wear analyses, it is likely that level F has actually recorded a succession of short occupations, contrary to what is observed in the levels G and D. Those differences in the duration of site occupation are discussed in the light of previous sedimentological, zooarchaeological, and technological studies.

Dietary change in the Epi-Paleolithic and Neolithic of the Levant : the Sr/Ca evidence

Site catchment surveys, dental-wear analyses and conventional archaeological studies have provided a complex, often contradictory picture of the diet of Natufian and other Epi-Paleolithic peoples. In order to clarify the variability and possible diachronic changes in diet during this period, strontium-calcium ratios (Sr/Ca) were determined for all Kebaran, Natufian and PPNA sites in the Levant from which appropriate faunal and human samples were available. At all Kebaran and Early Natufian levels, including Kebara В and C, Hayonim Cave В and El Wad B, a moderate consumption of cereal foods was detectable. However the consumption of cereal foods was found to markedly decrease in the later Natufian at Mallaha and Nahal Oren. With the domestication of cereals in the PPNA, these foods were found to regain their previous importance in human diets.