To assess methods of sterilization in dental practices in Karachi and secondly to investigate methods of monitoring sterilization in dental practices in Karachi, Pakistan. Cross-sectional, descriptive study. Dental colleges, hospitals and private clinics of Karachi, Pakistan, from January to March 2013. A total of 251 questionnaires were obtained. Descriptive statistics were computed and differences between groups were assessed through chi-square test using Statistical Package for the Social Sciences (SPSS) version 16.0. P-value < 0.05 was taken as statistically significant. Autoclave, used by 155 (61.8%) dentists was the most common method of sterilization followed by more than one method, 65 (25.9%); dry heat, 24 (9.6%); and cold sterilization, 7 (2.8%). Majority of dentists, 126 (50.1%), never monitored sterilization and those who did monitored mostly monthly. Statistically significant difference was found amongst the three groups of dentists monitoring sterilization (p=0.09) and methods of sterilization (p < 0.01). Statistically significant difference was found in infection control practices of specialists, postgraduate trainees and general dentists regarding method of monitoring sterilization with majority of dentists never monitoring sterilization.