Dental Deposits Research Articles

Changes in oral microbiota at different stages of active orthodontic treatment

INTRODUCTION: There are no sufficient numbers of studies in the modern literature devoted to investigation of the relationship between the use of fixed orthodontic appliances and changes in the qualitative and quantitative bacterial composition at certain periods of orthodontic treatment. AIM: To identify changes in the qualitative and quantitative composition of the microbiocenosis in the area of installed fixed appliances at different periods of orthodontic treatment. MATERIALS AND METHODS: The study included 40 participants aged 18–44 years. All patients had a history of occlusion pathologies, dento-alveolar anomalies, and needed orthodontic treatment with use of fixed metal bracket systems. Before installing a fixed ligature vestibular metal bracket system (OC Orthodontics, USA), all patients underwent sanitation by a dental therapist, professional oral hygiene (removal of hard and soft dental deposits, tooth polishing), and, if necessary, extraction of wisdom teeth/wisdom tooth germs. The microbiocenosis was assessed in the area of intended placement of braces before orthodontic treatment, in the area of installation of braces in the period of adaptation to the fixed appliance (at 1 month after fixing the appliance) and in the period of active treatment (at 6 months after fixing the appliance). RESULTS: On the initial examination, the frequency of detection of stabilizing microflora was 81.25%, periodontopathogenic microflora — 18.75%. In the period of adaptation, the frequency of detection of stabilizing microflora was 56.25%, the frequency of detection of periodontopathogens increased to 43.75% (p = 0.052). In the phase of active orthodontic treatment, a decrease in the frequency of detection of periodontopathogenic bacteria to 40.96% was observed, and the frequency of detection of stabilizing microflora was 59.04% (p = 0.007). The microbial number before the installation of fixed orthodontic appliances was 105 ± 102 CFU/cm2, in the adaptation phase — 107 ± 102 CFU/cm2, in the period of active treatment — 105 ± 102 CFU/cm2 (p = 0.008). CONCLUSION: In the course of clinical and laboratory study, species and quantitative composition of the oral microbiocenosis was found to have characteristic features at different stages of the orthodontic treatment: before installation of fixed orthodontic appliances, the so-called ‘stabilizing’ microorganisms prevail over representatives of the group of periodontopathogens, in the period of adaptation, the proportion of periodontopathogens increases significantly with a decrease in the proportion of stabilizing microflora, in the phase of active orthodontic treatment their ratio normalizes.

Morphological study of hard dental tissue condition with various types of dental deposits

Today, it has been established that the primary factor in preventing dental caries is controlling the level and type of dental deposits. However, the literature has not yet addressed the specific morphological changes in the hard dental tissues when non-mineralized and mineralized dental plaque is present on the tooth surface. The aim of this study was to examine the microscopic structure of the cuticle and hard dental tissues and to assess the X-ray spectral changes associated with different types of dental deposits. Epimicroscopic and X-ray spectral examinations were performed on 32 teeth with various types of supragingival deposits, extracted for orthodontic and surgical indications. Each tooth was cut in the vestibulo-oral direction using a diamond bur at low speed, then one half was polished, stained with PAS-Alcian blue, and photographed at different magnifications in epiprojection. Samples were carbon-coated in a vacuum chamber, and the inorganic components in the cuticle and mineralized deposits were assessed using the Link AN 10.85s micro X-ray analyzer. Thin sections of the other tooth halves were prepared, stained with PAS-Alcian blue, and examined under the “Olympus” microscope at various magnifications. Statistical analysis was performed using standard parametric methods with “Statistica 6.0” and “Microsoft Excel 2002” software. Significant findings in the hard dental tissues included the presence of non-mineralized (soft plaque, dental biofilm), mineralized (dental calculus), and smoker's plaque. In areas with soft plaque, enamel prisms remained intact, with no changes in the enamel-dentin junction or dentin, though the cuticle was nearly absent. In areas with dental biofilm, enamel thickening, the absence of characteristic enamel prism patterns, fragmentation of the reticular layer, and formation of interglobular dentin were observed. Morphological changes in mineralized deposits included enamel thinning, prism disorganization, localized hypermineralization, destroyed enamel-dentin junctions, and “dead tracts” in the dentin. In cases with smoker's plaque, nicotine pigment accumulated in Retzius line biomineralization zones, along with thickening of the reticular layer and “dead tracts” in the deeper dentin layers. A comparison of the main inorganic components (Ca, P, Mg, Ca/P) in the cuticle and mineralized deposits showed significant differences in phosphate, magnesium, and Ca/P ratios, indicating disrupted metabolic processes at the “enamel-oral fluid” interface, promoting calculus formation. Thus, the morphological and X-ray spectral changes observed in the enamel and surrounding tissues provide an objective assessment of tissue condition with various types of deposits. These findings should be considered in selecting a scaling method.

The effectiveness of non-drug correction of chronic periodontitis in patients who have undergone a new coronavirus infection on the severity of oxidative stress

Purpose of the study. To evaluate the effect of the use of non-drug correction of chronic periodontitis in patients who have undergone a new coronavirus infection on the severity of oxidative stress. Material and methods. A total of 156 patients with CP aged 26 to 63 years participated in the study (the average age was 44.7±6.15 years). When dividing patients by gender, the majority were men — 86 patients (55.1 %). 126 patients out of 156 examined had a history of COVID-19 with a prescription of 1 to 6 months. At the first stage, by stratified randomization, all patients (156 patients), depending on the presence of a history of NCI, were divided into two unequal groups: 126 patients with CP who underwent COVID-19, and 30 patients with CP who did not have NCI. At the second stage, 126 patients with CP who underwent COVID-19 were divided into 4 groups using simple fixed randomization: a control group, two comparison groups and a main one. The control group received standard periodontal therapy (SPT), regulated by clinical guidelines. In comparison groups 1 and 2, SPT was supplemented, respectively, with applications of liposomal gel with DHA and NILI. In the main group, a complex of liposomal gel and NILI was used as an adjunct to SPT. Results. The SPT included professional oral hygiene with ultrasonic scalers and curettes of the Piezon Master 700 device (EMS, Switzerland). The removal of dental deposits was carried out using air-abrasive technology Aig-Flow (EMS, Switzerland). Individual oral hygiene was trained and monitored. Conclusion. The factors contributing to the development of inflammatory periodontal diseases, namely carious cavities, wedge-shaped defects, overhanging edges of fillings, were also eliminated.

ОЦЕНКА ЭФФЕКТИВНОСТИ ПРИМЕНЕНИЯ ПРИ ВОСПАЛИТЕЛЬНЫХ ЗАБОЛЕВАНИЯХ ПАРОДОНТА СПРЕЯ НА ОСНОВЕ МУКОПРОТЕКТОРА: РЕОЛОГИЧЕСКИЕ ХАРАКТЕРИСТИКИ СМЕШАННОЙ СЛЮНЫ

Subject. The subject of the study is a mucoprotective spray, mixed saliva. Objectives. The aim of the study was to evaluate the effectiveness of use of the mucorotective spray on periodontal inflammation by analyzing dynamic changes in the rheological properties of mixed saliva. Methodology. The study is conducted by the Department of Therapeutic Dentistry and Propaedeutics of Dental Diseases, the Department of General Chemistry, Ural State Medical University. The research objects are: 1 – the spray with mucoprotector, titanium glycerosolvate aquacomplex used as an “inorganic nanoparticle” with high delivery capabilites into deep tissue layers and activity enhancement of anti–inflammatory, antimicrobial and regenerating components of the spray; 2 – the spray without titanium glycerosolvate aquacomple (aqueous solution), placebo. The study involved 30 dental volunteer students whose average age was 20.5 ± 0.95 years (12 young men, 18 young women) with periodontal inflammation. Two groups were formed consisting of 15 people each who used the study sprays during two weeks. The dynamic changes in the rheological parameters of mixed saliva were determined by the following methods: measuring salivary flow rates, viscosity measurements, evaluation of surface tension of mixed saliva to characterize its rinsing function and cleansing properties. Conclusions. Analysis of the findings shows high effectiveness of use of the mucorotective spray on the basis of titanium glycerosolvate aquacomplex with anti-inflammatory effect, that is confirmed by the dynamic changes in the hygiene index and the PMA index (p ⩽ 0.05). The spray has got high trans-mucus penetration and good tolerability; it is easy to use and economically affordable. The analysis of changes in the rheological parameters of mixed saliva in patients from the first group during the application course of the spray on the basis of the the titanium glycerosolvate aquacomplex mucoprotector showed increased saliva flow rates and decreased viscosity of saliva. These enhance the rheological propertiies of mixed saliva, its rinsing function and cleansing properties, as well reduce the risk of tooth decay and gum swelling, and prevent dental deposits in patients with periodontal inflammation.

Oral Health in Patients Hospitalized Because of Ischemic Stroke.

Background/Objectives: Maintenance of good oral health is relevant to overall health and quality of life. Results of many analyses showed that stroke patients had worse oral health than the control population. The aim of this study was a clinical assessment of oral condition in post-stroke patients and a healthy population. Methods: Oral health was assessed in stroke patients on the first day of ischemic stroke, and in a control group of healthy subjects. The number of teeth, the presence of active carious foci, fillings, and prosthetic restorations were evaluated. To assess oral hygiene, the Approximal Plaque Index (API) was used. In periodontal examinations, the presence of dental deposits, the depth of the existing periodontal pockets, tooth mobility, and the Sulcus Bleeding Index (SBI) during probing were assessed. Results: Significantly higher mean values of Decayed Teeth (DT), Missing Teeth (MT), and Decayed, Missing, and Filled Teeth (DMFT) indices were recorded in the study group. The incidence of dental caries, API, and SBI was also significantly higher in the study group. The study and control groups did not differ significantly in the average number of pockets 3 mm deep and deeper and in the frequency of having prosthetic restorations. Conclusions: Oral health and the level of oral hygiene in patients hospitalized because of ischemic stroke, in comparison with that in a healthy population, is not satisfactory. Active interdisciplinary collaboration between various medical specialists in the therapy of patients with general illnesses, including stroke, is strongly recommended.

The Effects of Air Abrasive Polishing on the Release of Iron Ions from Various Orthodontic Arch Wires (Stainless Steel and Nickel Titanium)

Orthodontic treatment with has been increasingly demanded. However, when using fixed appliances for orthodontic treatment, oral hygiene is compromised, and there is a higher chance of developing dental stains, plaque-related diseases, and corrosion-related problems. Air abrasive polishing had a superior effect over the conventional method in removing dental deposits, however, its effect on corrosion process was not investigated thoroughly in details. This study designed to assess the effects of air polishing onion release and surface micromorphology of stainless steel and Nickel titanium arch wires. A total of 288 ( stainless-steel , coated stainless steel , Nickel titanium and coated Nickel titanium rectangular arch wires ) of one brand ( The Ultimate Wire) ™ From IOS (International Orthodontic Services, Stafford, USA), (0.016 * 0.022 ) were subjected to varying air abrasion polishing periods (5, 10, and 20 seconds), after which they were immersed in artificial saliva with a pH of 6.75 and incubated at 37°C for 28 days. Ion release was measured using an atomic absorption spectrophotometer at 7 days, 14 days, and 28 days. The cumulative effect was then calculated. After polishing the chosen sample, corrosion and surface changes were evaluated using a scanning electron microscope (SEM). The significant difference between the analysed groups was found using an analysis of variance (ANOVA) and the LSD test, with a significance level of p 0.05. The results revealed that all types of arch wire showed a significant amount of release in the tested ions and the amounts of nickel and iron ions released was higher than chromium ion. Additionally, the longer the polishing time the higher the number of ions release that’s yields the level of significance, air abrasion polishing increased surface roughness and surface pits and crevices.It may be concluded that the air polishing method enhanced the amount of ion release to a subtoxic level and could be employed in adult patients with a lengthy time between visits.This study was performed to:1. Evaluate the effect of calcium carbonate airborne particles abrasion with different polishing times 5, 10 and 20 seconds on (Fe) ions release from orthodontic arch wires. 2. Assess the surface micromorphology of orthodontic arch wires after air polishing procedure.

Clinical study of cervical caries treatment the effectiveness by assessing composite restorations condition

Background. Cervical caries is a fairly common teeth pathology and its treatment is carried out in complicated clinical conditions. Therefore, increasing the term of functioning of composite restorations is an urgent task. Aim. Identification of the effectiveness of a differential approach to dissection of hard dental tissues depending on the depth of enamel microcracks and implementation of professional hygiene in the treatment of cervical caries in the short- and long-term periods. Materials & Methods. The study involved 50 people (average age [23.28±5.52] years) in whom cervical caries was treated in 72 teeth. All patients were divided into three equal groups based on the number of restorations according to the selected dissection technique and the chara­cteristics of professional hygiene. The dissection technique depended on the depth of the enamel microcracks in Main Group I (MG I), the dissection was carried out within the limits of clinically intact hard dental tissues in Control Group (CG) and Main Group II (MG II). The dental biofilm was removed from the vestibular surface of the teeth by the method of air-abrasive cleaning using the erythritol-based powder in MG I and MG II, the vestibular surface of the tooth was cleaned of dental deposits with a polishing paste without fluoride in CG. The quality of restorations was assessed based on the United States Public Health Service (USPHS) criteria on the day of restoration, 6 and 12 months later. The effectiveness of the prescribed treatment was identified by the dynamic indicators of the dental pulp electroexcitability and the electrical conductivity of the enamel. Results. There were no partial or completely destroyed restorations in all terms of the observation period. Composite restorations were preserved according to the criteria of "anatomical shape" and "color matching". One year later restoration defects were absent in 61 teeth (84.72%): in MG I – in 22 (91.67%), in CG – in 19 (79.17%), in MG II – in 20 (83.33%). Recurrent caries and symptoms of hypersensitivity were not identified in MG I. A slightly greater effectiveness of the treatment was noted in MG II group in comparison with CG (by 4.16%) that confirms the importance of removing the dental biofilm before restoration by the method of air-abrasive clea­ning using erythritol. Conclusions. The analysis of the state of restorations in the long-term period showed that the dissection technique and the features of professional hygiene affected their quality before restoration. The number of high-quality composite restorations was by 10.42% more on average in MG I than in the other groups. The obtained results make it possible to recommend the proposed method for using in practical dentistry. Keywords: electroodontology, electroodontometry, microcracks.

FLUORESCENT DIAGNOSTIC METHOD AS A TOOL FOR COMPREHENSIVE EVALUATION OF THE HYGIENE OF NATURAL TEETH AND FIXED DENTAL PROSTHESIS CONSTRUCTIONS

This study focuses on the critical need for an oral care, especially when using fixed bridge prostheses. The analysis and impact of insufficient care for oral hygiene on the development of dental diseases and the need to develop effective methods for its assessment have been carried out. The research consists of the improvement of fluorescent diagnostics in orthopedic dentistry, as well as, in the development of an index for the assessment of the hygiene of natural teeth and fixed denture structures based on an in vivo experiment. This aims to improve the diagnosis of the condition of the oral cavity in patients with fixed prostheses, contributing to the improvement of the quality of their orthopedic treatment. Materials and methods: The use of fluorescent diagnostics with Rhodamine G dye allows to identify the presence of dental deposits under the influence of ultraviolet light. The technique involves treating teeth and dentures with a solution that enhances the visibility of dental plaque under ultraviolet light. Research results: The obtained research data confirm the high efficiency of the fluorescent technique in the detection of dental deposits, both on natural teeth and on the surfaces of fixed prostheses. Digital analysis allows you to visualize the specific graphic characteristics of the biofilm, which facilitates the adaptation of an individual approach. Conclusions: The conducted study demonstrates that the improved fluorescent method was extremely effective in determining the state of oral hygiene, effective both for the analysis of natural teeth and fixed prostheses. The proposed method allows you to determine the hygienic condition in detail, makes a significant contribution to the prevention of dental diseases and improves the optimization of medical interventions. The technique is easy to use, and the corresponding software is available for dentists. Further expansion of observations for the use of this technique in patients with a different orthopedic spectrum of needs is expected.

INDEX-BASED ASSESSMENT ALGORITHM FOR ORAL HYGIENE IN WEARERS OF FIXED DENTAL BRIDGES

Introduction: The study focuses on the importance of maintaining oral hygiene, especially in wearers of fixed dental bridge. The impact of inadequate oral hygiene care on the development of dental diseases and the necessity for developing effective methods for its assessment are examined. Research objective: The aim of this study is to refine the fluorescent diagnostic method in orthopedic dentistry and to develop an index for assessing the hygienic status of natural teeth and fixed dental prostheses through an in vivo experiment. This method is intended to improve the diagnosis of oral conditions in wearers of fixed prostheses, ultimately enhancing the quality of their orthopedic treatment. Materials and methods: The study is based on the application of fluorescent diagnostics using Rhodamine B dye, which, when exposed to ultraviolet light, allows for the detection of dental deposits. The technique involves treating the surfaces of teeth and prostheses with a special solution that enhances the visibility of dental plaque under ultraviolet illumination. Research results: The experimental results demonstrated the high efficiency of the fluorescent method in detecting dental deposits both on the enamel of natural teeth and on the ceramic surface of fixed dentures. The digital analysis method allows for obtaining specific graphical indicators of the biofilm area, enhancing the relevance of an individual approach. Conclusions: Conclusions: The experimental study confirmed the high efficiency of the refined fluorescent method for assessing the hygienic status of the oral cavity, applicable to both natural teeth and fixed orthopedic constructions. This technique enables accurate assessment of hygiene status, facilitating effective prevention of dental diseases and optimization of treatment procedures. The method is convenient to use, and the software is accessible for clinicians. Further observations on the outcomes of this technique in patients with various orthopedic profiles are planned to expand its applicability.

Effects of a new magnetostrictive ultrasonic scaler and a traditional piezoelectric ultrasonic scaler on root surfaces and patient complaints

Tooth wear and pain are the primary concerns of patients undergoing periodontal scaling. The aims of this study were to compare the effects of a new magnetostrictive ultrasonic scaler and a traditional piezoelectric ultrasonic scaler on tooth surface roughness and calculus removal and to determine their impacts on patient discomfort during supragingival cleaning. This article had two parts: an in vitro study and a clinical study. In the in vitro study, thirty teeth with subgingival calculus were randomly assigned to two scaling treatment groups: magnetostrictive scalers (n = 15) and piezoelectric scalers (n = 15). Surface roughness measurements were taken at baseline and after scaling, and the root samples were visualised by SEM after scaling. Additionally, a single-centre randomised split-mouth clinical trial was conducted. Eighty-five participants diagnosed with chronic gingivitis or periodontitis were randomly assigned to receive supragingival scaling. The magnetostrictive scaler was used in half of the mouths (n = 85), and the piezoelectric scaler was used in the other half of the mouths (n = 85). Data on pain, noise, and vibration were collected using a VAS questionnaire, and the operating time was recorded. In both in vitro and clinical studies, magnetostrictive scalers were reported to be more effective than piezoelectric scalers in removing dental deposits (P < 0.05). Additionally, the root surface after scaling with the magnetostrictive scaler was smoother than that after scaling with the piezoelectric scaler in the in vitro study (P = 0.02). SEM examination also revealed that fewer dental materials were lost after instrumentation with the magnetostrictive scaler than after instrumentation with the piezoelectric scaler. Piezoelectric scalers caused less discomfort to patients in terms of pain, noise, and vibration than magnetostrictive scalers (P < 0.05). According to this clinical study, the magnetostrictive scaler caused more discomfort during supragingival scaling than the piezoelectric scaler. Moreover, the magnetostrictive scaler was also more efficient and produced a smoother root surface with less material loss after scaling than the piezoelectric scaler, as demonstrated in the in vitro study.

Effect of air abrasive polishing on nickel release, stainless steel corrosion, and nickel-titanium archwires.

Orthodontic treatment is becoming more and more popular. However, using fixed orthodontic devices for treatment affects oral hygiene and raises the risk of corrosion, plaque-related illnesses, and dental discoloration-related issues. Air abrasive polishing has a superior effect over the conventional method in removing dental deposits. Using fixed orthodontic appliances affects oral hygiene and raises the risk of diseases caused by plaque, tooth discoloration, and corrosion, as well as corrosion by ions. This study evaluated the impact of air polishing on nickel ion release and corrosion from stainless steel, nickel-titanium, coated stainless steel, and coated nickel-titanium. A total of 288 (stainless steel, coated stainless-steel, nickel-titanium, and coated nickel-titanium rectangular archwires) of one brand were subjected to varying air abrasion polishing times (5, 10, and 20 seconds). Then, they were submerged in artificial saliva with a pH of 6.75 and incubated for 28 days at 37 °C. The release of nickel ions (Ni2+) was measured using an atomic absorption spectrophotometer at 7, 14, and 28 days to estimate the cumulative effect. The corrosion of the test-selected samples and surface alterations was evaluated using scanning electron microscopy (SEM). Prolonged polishing significantly increased Ni2+ release and corrosion. Archwires made of coated stainless steel exhibited the least amount of Ni2+ release. The air polishing process increased the Ni2+ release at a subtoxic level and could be used on adult patients with long gaps between visits with a polishing period of 5 seconds.

ORTHOPEDIC REHABILITATION OF PATIENTS WITH CHRONIC GENERALIZED PERIODONTISIS USING DENTAL IMPLANTS

One of the most common methods of correcting dental defects used in modern dentistry is dental implantation. However, in conditions of periodontal pathology, planning and carrying out dental implantation causes certain difficulties. The aim of the work was to analyze the clinical effectiveness of orthopedic rehabilitation based on implants in patients with chronic periodontitis. The task of the study was to study and compare the indicators of implant stability, marginal bone tissue loss and postoperative complications in patients with chronic generalized periodontitis under the conditions of using the technique of immediate and delayed dental implantation.&#x0D; The object and methods of research. To achieve the goal, the authors formed two observation groups, in which the replacement of tooth row defects with the help of dental implants was performed in patients with generalized periodontitis of the I-II degree of severity. The first group consisted of 10 patients who underwent direct implantation and immediate functional loading. There were 10 patients in the second group, after delayed implantation, loading occurred in the generally accepted terms (2-3 months on the lower jaw and 4-5 months on the upper).&#x0D; The stability of the implants was determined using the Osstell ISQ device. A comparison of the indicators of implant stability with different implantation protocols and functional load: immediate and delayed load was carried out.&#x0D; Conclusion. The maximum value of stability of implants is registered after one year. This fact is proven in a statistically significant increase (p&lt;0.05) in the stability of the implant in both groups.X-ray evaluation of bone tissue resorption in the cervical area after the first year of loading indicates certain risks of direct implantation and immediate functional loading.&#x0D; Thus, effective prosthetics for patients with generalized periodontitis is determined by a personalized approach to each individual case, taking into account local factors and individual characteristics of the body. Most often, it is unsatisfactory oral hygiene that leads to an increase in the amount of dental deposits and creates prerequisites for the exacerbation of the inflammatory-dystrophic process in the periodontal tissues.

ИЗУЧЕНИЕ ОЧИЩАЮЩЕГО ЭФФЕКТА СПРЕЯ ДЛЯ ПРОФИЛАКТИКИ КАРИЕСА, СОДЕРЖАЩЕГО STREPTOCOCUS THERMOPHILUS ЛИЗАТ И ПРЕБИОТИКИ, КАК ПРОМЕЖУТОЧНОГО СРЕДСТВА ГИГИЕНЫ РТА

Background. The problem of controlling the formation and development of dental plaque as a risk factor for the development of dental diseases is leading in preventing a decrease in the level of oral hygiene. In this regard, it remains relevant to study the preventive effects of liquid oral hygiene products with an anti-carious effect. Spray for the prevention of caries «DentaBalance®» synbiotic complex including lysate from Streptococcus Thermophilus and prebiotics, plant extracts that prevent the adhesion of bacteria to the surface of the teeth. At the same time, the metabolism of microorganisms that form plaque is disrupted, leading to their death, and the amount of acids produced by bacteria is also reduced, which increases the level of dental health.&#x0D; Material and methods. In the conditions of the clinic, a spray for the prevention of caries was tested in order to determine its cleansing effect as an intermediate oral hygiene product used during the day. The spray for the prevention of caries «DentaBalance®» synbiotic complex was used by probants independently 2 times a day for 30 days for intermediate use during the day. Repeated examinations were carried out every two weeks, for 4 weeks. The control of the cleansing effect of the intermediate liquid oral hygiene product was carried out for 30 days. The assessment was carried out according to the Green-Vermillion index using indicators of dental deposits.&#x0D; Results. Based on the results of the study, the cleansing effect according to the Green - Vermillion index was established in persons who used the spray for the prevention of caries «DentaBalance®» synbiotic complex during the day, by the end of 4 weeks was 50.66 ± 0.51. The data obtained were confirmed by statistical processing of the results of the study.&#x0D; Conclusion. It has been established that the spray for the prevention of caries «DentaBalance®» synbiotic complex has a pronounced cleansing effect when used during the day, which is confirmed by its cleansing effectiveness. Batch spraying of liquid hygiene products in the dosage form of the spray effectively delivers microparticles with their active components, and also ensures its mobility in use.

A water additive with pomegranate can reduce dental plaque and calculus accumulation in dogs.

Oral homecare plays a major part in dental disease prevention but it can be difficult to perform and time-consuming. Furthermore, the product used can be of limited efficiency. The goal of this study was to assess the efficacy of a water additive to limit the accumulation of plaque and calculus in dogs. Forty dogs were selected and randomly allocated to one of the two groups after scaling and polishing on day 0. The control group received no oral hygiene while the second group received the water additive (Vet Aquadent® FR3SH™, Virbac) every day. After 30 days, plaque and calculus accumulations were evaluated under anesthesia. The Gingival Bleeding Index (GBI) was assessed on days 0 and 30. On day 30, the plaque and calculus indices were significantly smaller (p < 0.05) in the Aquadent group compared to the control group with median (Q1-Q3) scores of 1.22 (0.99-1.44) vs. 2.31 (1.65-3.86), respectively for plaque and 0.25 (0.15-0.42) vs. 0.33 (0.32-0.69) for calculus. Between day 0 and day 30, the GBI significantly decreased in the control group [from 0.39 (0.21-0.56) to 0.19 (0.08-0.29)] and in the Aquadent group [from 0.33 (0.18-0.47) to 0.00 (0.00-0.00)] but the decrease was significantly greater in the Aquadent group. These results show for the first time that the water additive tested can reduce dental deposit accumulation in dogs and improve gingival health. It can be recommended after a dental cleaning, especially to owners who are reluctant to provide dental care at home due to a lack of time or convenience.

EVALUATION OF LOCAL THERAPEUTIC AGENTS IN ANIMALS WITH EXPERIMENTAL PERIODONTITIS

Main etiological factor in the development of generalized periodontitis is considered to be the presence of periodontopathogens of the red complex, namely Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Bacteroides forsythus in the oral cavity. Among the widely used local therapeutic agents for periodontal diseases, researchers highlight chlorhexidine. Chlorhexidine is available in the form of toothpaste, mouthwashes, gels, sprays, and inserts (PerioChip). In dentistry, the antiseptic is commonly applied in concentrations of 0.2%, 0.12%, and 0.06%. The aim of this study was to propose and evaluate new local therapeutic agents in experimental animals with modeled chronic periodontitis. The study included 36 experimental animals. We suggested using an insert with subgingival application, soaked with chlorhexidinebased official products – Dentaid Perio-Aid Gel and Perio-Aid Protect Gel. The animals were divided into groups as follows: Group I intact rats, Group II modeled periodontal pathology without treatment on the 7th day of modeling, Group III rats with experimental periodontitis without treatment on the 12th day of modeled pathology, Group IV rats with experimental periodontitis treated with an insert soaked in 0.12% chlorhexidine gel (Dentaid Perio-Aid Gel), and Group V rats with experimental periodontitis treated with an insert soaked in 0.2% chlorhexidine gel + hyaluronic acid (PERIO-AID Protect Gel).&#x0D; The experimental periodontitis was induced using an ultrasonic device. On the 7th and 12th days of the experiment, the rats from Groups IV and V were treated according to the specified scheme, preceded by removal of dental deposits using manual instruments. Group IV was treated with an insert with subgingival application soaked in Dentaid Perio-Aid Gel, and Group V was treated with an insert soaked in Perio-Aid Protect Gel. Euthanasia of the rats and sampling of blood and periodontal tissues (gingiva, alveolar process) were performed on the 7th and 12th days in the animals with modeled periodontitis. The assessment of the modeled pathological process was based on clinical examination and biochemical analysis. After the experiment, it was observed that the probable reduction in antioxidant defense during the treatment of experimental animals with Dentaid Perio-Aid Gel and Perio-Aid Protect Gel, applied on the insert, was associated with a decrease in lipid peroxidation. At the same time, alkaline phosphatase activity increased, both in serum and in the homogenate, which may indicate activation of bone formation processes.

Occurrences of Dental Diseases in Dogs in a Teaching Animal Hospital, Morogoro, Tanzania

Dental diseases are common in dogs with a prevalence of more than 80% in some breeds of dogs. They cause damage to oral health and may act as a source of bacteria causing other diseases such as endocarditis, pneumonia and arthritis. A cross section study was conducted to determine the current status on the occurrence of dental diseases in dogs attended at Sokoine University of Agriculture (SUA) Teaching Animal Hospital in Morogoro, Tanzania. A total of 87 dogs were examined macroscopically for the presence of dental diseases. Dogs examined in this study ranged from medium (93.1%) to large (6.9%) sized, and up to 73.6% were mixed-breed. The minimum and maximum ages of the dogs were 6 and 72 months, respectively, with majority being females (60.9%). The proportions of intact, spayed and castrated were 92%, 5.8% and 2.3%, respectively. Of the 87 dogs examined, 62.1% had different kinds of dental diseases. The frequently observed dental diseases were; dental deposits (43.7%), missing teeth (24.1%), periodontal disease (20.7%), dental attrition (16.1%), and dental abrasion (10.3%). Further, it was noted that none of the dogs had been previous underwent routine dental check-up or anaesthetised for dental cleaning. This study indicates that dental diseases are frequent in dogs attended at SUA Teaching Animal Hospital. Therefore, it is recommended that routine dental examination should be performed in dogs presented at the Teaching Animal Hospital. Further, there is a need for educating dog breeders and owners on the importance of dental home care.

Evaluating the Efficacy of Synbiotic Mouthwash in Patients with Dyscirculatory Encephalopathy: Observational Clinical Study

Background. At present, the priority direction of medical science and practice is the prevention of dental diseases, especially in patients with somatic pathology. Patients with dyscirculatory encephalopathy develop a complex of neurological disorders, accompanied by impaired motor functions of hands, lips and chewing muscles, which leads to ineffective cleansing of the oral cavity from food residues and formation of soft and mineralized dental deposits on the dental surface. Therefore, these patients should use additional personal hygiene products.Objective. To evaluate the efficacy of hygienic care in patients with dyscirculatory encephalopathy using mouthwash with synbiotic complex.Methods. An observational clinical study was conducted on the basis of Neurology Unit No. 2 of Research Center of Neurology, Department of Cariology and Endodontics and Department of Biological Chemistry, A.I. Yevdokimov Moscow State Medical and Dental University. The study enrolled 82 patients aged from 45 to 74 years with cerebrovascular disease, dyscirculatory encephalopathy, decompensation (I68.7, according to ICD-10). Patients were divided into two study groups: group 1 (n = 44) — patients who used mouthwash with synbiotic complex; group 2 (n = 38) — patients who did not use mouthwash. The efficacy of mouthwash with synbiotic complex was assessed by using an index score of a simplified Greene Vermillion Oral Hygiene Index (OHI-S), Russell Periodontal Index (PI), Papillary Bleeding Index (PBI), and physicochemical parameters of the oral fluid. Statistical processing of the results was carried out using Stat Plus 7 (Analyst Soft Inc., USA).Results. The comparative analysis proved the efficacy of mouthwash with synbiotic complex. The hygienic status improved to (1.50 ± 0.12) points, the state of periodontal tissues — to (PI = (1.94 ± 1.45) points; PBI = (1.12 ± 0.11) points). The hydrogen index (pH) of the oral fluid decreased to weakly alkaline values — (7.92 ± 0.22) points; the activity of alkaline phosphatase decreased by a factor of 1.4, lactate dehydrogenase — by half, aminotransferases — by a factor of 1.3, and D-dimer — by a factor of 1.2.Conclusion. The obtained results demonstrate the efficacy of mouthwash as an additional means for personal hygiene. Mouthwash with synbiotic complex should be included in the daily oral hygiene care for patients with dyscirculatory encephalopathy.

Features of trophic processes occurring in the bone tissue after the operation of dental implantation

Relevance: A key aspect of success after dental implant surgery is obtaining reliable osseointegration of the installed dental implant. In modern literary sources, the problem of consistency of osseointegration after the installation of dental implants is quite often touched upon. A number of authors argue that the quality of osseointegration of a dental implant depends primarily on its primary stability. Long-term studies show that bone tissue permanently undergoes various resorption and recovery processes [1, 2]. Purpose to determine the effectiveness of alkaline phosphatase and collagen protein osteocalcin in oral fluid after dental implantation. Materials and methods: Clinical studies were carried out on the basis of the Clinics of the Samara State Medical University of the Ministry of Health of Russia. A total of 240 patients with a diagnosis of partial absence of teeth were operated on. All patients received dental implants. The median age was 44.2 years (24 to 62 years; 182 women and 58 men). Results and discussion: At the stage of orthopedic rehabilitation, most patients had a low index of oral hygiene, OHI-S averaged (2.63 0.27) c.u. All patients suffering from poor oral hygiene were recommended professional hygiene with the removal of soft and hard dental deposits. At the stage of dental implantation, the OHI-S and Mulleman index was (0.66 0.03) and (0.49 0.03) c.u. respectively. Conclusions: The results of the study showed that the increase in the titer of alkaline phosphatase and the collagen protein osteocalcin was statistically insignificant. The level of acid phosphatase titer in the oral fluid reaches its critical value two weeks after the dental implantation operation. Studies have shown that this indicator remains active up to six months after surgery. This indicator can be used to interpret various emerging trophic processes occurring in bone tissue after dental implantation.

Modern understanding of endoscopy technology at a periodontal appointment: a systematic review

Relevance. Periodontitis is a common chronic infectious and inflammatory disease. Multiple microorganisms, including periodontal pathogens in the dental biofilm, are the principal reason for inflammatory periodontal diseases. The initial stage of periodontitis treatment involves the mechanical removal of dental deposits from the tooth surface. Subgingival scaling is technically complex due to the limited visualization. An experienced clinician does not always have a chance to thoroughly treat all roo ts’ surfaces and remove all plaque and tartar.Modern technology, e.g., Perioscopy, enables illumination and visualization of periodontal pockets and their content. Thus, dental endoscopy technology practicability determination requires the study of the systematization of a large initial data array.Materials and methods. Publications were searched and studied in seven electronic databases PubMed, Google Search, Embase, Web of Science, ScienceDirect, and SciELO II eLibrary. The study reviewed the articles published from 2000 to 2022, available in full text, and assessed for relevance. The search resulted in 119 selected publications. Based on the inclusion criteria, we selected 44 articles, which included 42 clinical trials and two reviews. The study methodology meets the requirements for systematic reviews (PRISMA).Results. High-quality visualization allows for the operating field control enabling access to hard-to-reach areas and improves periodontal treatment outcomes. Closed periodontal scaling, the most commonly used non-surgical inflammatory periodontal disease treatment technique, is based on the dentist’s tactile sensations and experience. Due to the lack of visual control, even an experienced practitioner may not always effectively treat all surfaces or remove all plaque and tartar. The examination with the endoscope (Perioscopy) after the instrumentation reveals areas of tartar and biofilm remains, which may lead to further periodontal destruction and future surgical treatment. The article presents the studies proving the sufficient effectiveness of a dental endoscope for periodontal disease treatment. It is of note that the endoscope significantly increases the treatment quality in cases with deep pockets and severe periodontitis.Conclusion. Endoscopic imaging of dental deposits and pocket content indirectly reduces the risk of recurrence and complications of inflammatory periodontal diseases. The treatment of patients with moderate and severe periodontitis requires the development of algorithms for the management of such patients with the mandatory use of an endoscope.

Histomorphological study of gingival recession in the experiment

Among periodontal diseases, one of the most common forms is gingival recession, which is a progressive displacement of the marginal gingiva in the apical direction. Studies by a number of authors indicate an increase in the prevalence and intensity of recession from 9.7 % to 99.3 % (at the age of 15 years to adult age, respectively) [1–3]. The reasons for the development of recession may be the following factors: anatomical and physiological features of the structure of the alveolar process, anomalies of teeth, dentition and dental occlusion, microbial factor (hard and soft dental deposits), acute or chronic gum injury, bad habits, and iatrogenic causes [10, 9, 11, 17]. Gingival recession is the cause of dentin sensitivity, as well as a factor in additional plaque retention, which stimulates further loss of periodontal attachment [11, 17]. Any aesthetic violations significantly reduce the quality of life. In this aspect, gum recession, which causes exposure of the roots of the teeth, has an extremely negative effect on the psycho-emotional state of a patient. An integrated approach to the problem under consideration is of great importance, in particular, in elderly and senile people, where systemic disorders of mineral metabolism often come to the fore [4, 8, 12, 14, 15, 16]. Experimental modeling of periodontal pathologies is of great clinical and rehabilitation significance [6, 7, 10, 11, 13]. Currently, there are various methods of treating gingival recession using a complex of therapeutic, surgical, orthodontic, and orthopedic methods. However, the results of observations and monitoring of the effectiveness of known methods of treating gingival recession do not indicate long-term restoration of periodontal tissues [5, 10, 11, 17]. Thus, the foregoing indicates the feasibility of conducting experimental and clinical studies to find methods aimed at activating recovery processes in periodontal tissues and allowing to increase the effectiveness of treatment of patients with gingival recession.