Ring spinning frame is the most common and widely used technique for the production of yarn. This yarn subsequently utilized for the production of fabrics. Recently the research studies are more emphasis to create the living comfort condition. The yarn package density has a vital role to determine the feel, comfort, bulkiness and dyeing characteristics of the spun yarn. The yarn packaging density is largely influenced by the yarn process parameters. Ring spinning technique is the most common and widely used for the production of yarn. This yarn subsequently utilized for the production of fabrics. Recently the research studies are more emphasis to create the living comfort condition. The yarn package density is a vital role to determine the feel, comfort, bulkiness and dyeing characteristics of the spun yarn. It is largely influenced by the yarn manufacturing techniques and raw material used. The effects of spinning process parameters on packing density will be helpful to predetermine the yarn comfort behaviour prior to the complete the fabric manufacturing process. Mechanical behaviour of the staple yarn depends on the fiber characteristics and the yarn structure i.e. the arrangement of the individual fiber on the cross-section of the yarn. The fiber distribution on the cross section of the yarn and packing density of yarn has been investigated. The study provides how the spinning process parameters affect the internal structure of ring spun yarn. The aim of the study is to improve the package density of the yarn to achieve dynamometric and comfort properties of yarn to attract a wide variety of customers. Yarn Packing Density represents inter-fiber distance and friction, hence controlling the mechanical and comfort properties of the yarn. The main objective of this study is to control the packing density of yarn during the spinning process. In this work the 100 percent cotton used as raw material and spun on a ring spinning machine. The 135 ring yarns samples have been produced from three different types of yarn counts include (Ne 8, 16 and 24) with nine different samples of identical counts were produced. The three different variables within the count sample are TPI and spindle speed and count. Yarn packing density was calculated by using the driven equation by the application of multiple regression models. The final result shows that packing density has directly related to the TPI, Spindle speed and count