It is important to clarify air flow induced bibration characteristics in hard disk drives in order to achieve ultra-high magnetic recording density. We studied flow induced flutter vibration in actual 2.5 inch HDD which has one disk and two magnetic heads. In this study, the disk flutter vibration as well as static pressure distribution between the top cover and disk were measured, changing the track following and seek modes of the magnetic head slider. From the experimental results, it was found that the disk flutter amplitude increases and its frequency decreases, when the magnetic head is operating on a disk. We also found that the mean pressure distribution was changed under various head operating conditions. As a result, it suggests that a disk flutter amplitude increase is due to the change of mean pressure distributions and disk flutter frequency decrease is caused by the effect of head-disk coupling vibration system.