Dense Hydroxyapatite Implants Research Articles

Efeitos do alcoolismo e da desintoxicação alcoólica sobre o reparo e biomecânica óssea

OBJETIVO: Avaliar os efeitos do consumo crônico de etanol e da desintoxicação alcoólica sobre a resistência mecânica do osso e neoformação óssea junto a implantes de hidroxiapatita densa (HAD) realizados em ratos. MÉTODOS: Foram utilizados 15 ratos divididos em três grupos, sendo controle (CT), alcoolista crônico (AC) e desintoxicado (DE). Após quatro semanas, foi realizada implantação de HAD na tíbia e produzida falha no osso parietal, em seguida o grupo AC continuaram a consumir etanol e o grupo DE iniciaram a desintoxicação. Ao completar 13 semanas os animais sofreram eutanásia, os ossos foram coletados para o processamento histomorfométrico e os fêmures encaminhados ao teste mecânico de resistência. RESULTADOS: Os animais do grupo AC apresentaram menores valores de neoformação óssea, de calcemia e resistência mecânica, quando comparado aos grupos CT e DE. Os animais dos grupos DE apresentaram valores superiores em todas as variáveis avaliadas em relação ao grupo AC. CONCLUSÃO: O consumo de etanol interferiu na osteogênese ao redor de implante de HAD, nos níveis de cálcio e na resistência mecânica óssea. A desintoxicação alcoólica se mostrou eficaz, pois aumentou à osteogênese e osseointegração da HAD, a calcemia e resistência mecânica óssea. Nivel de Evidência I, estudo terapeutico.

Study of bone formation around dense hydroxyapatite implants using light microscopy, image processing and confocal laser scanning microscopy

The bone reaction to hydroxyapatite (HA) implants inserted transcortically and extending into the medullary canal of rat tibiae was quantitatively assessed using light microscopy, confocal laser scanning microscopy and an image processing system. Sixty-five male rats (6 weeks old) were divided into two groups, 60 for histological observation and image analysis and five for time-labelling. In the histological observation, control sections of 168 days showed a few bone trabeculae in the fatty bone marrow, and Ti implants had become gradually encapsulated with a thick bone tissue layer; however, HA implants became almost completely encapsulated with a thin bone tissue layer during the 168 day experimental period. Histometrical analysis of the percent bone contact revealed that Ti implants showed a continuous increasing curve, and HA implants showed rapid increase in the initial healing period up to 14 days, with 96% bone contact reaching a plateau at 84 days after operation. There was a significant difference in the percent of bone contact between Ti and HA implants throughout the experimental period. Confocal laser scanning microscopic observations revealed the presence of calcein at the 14th day and only slight alizarin colour layer in the bone tissue at the 28th day, both indicating bone formation. These findings suggest that the activity of bone formation was higher at the 14th day than at the 28th day. Also, the percentage of bone contact of HA is superior to titanium throughout the experimental period, and the ascending patterns of both implants are quite different to each other.

Ａｐａｔｉｔｅ ｃｅｒａｍｉｃｓを用いた歯内骨内インプラントに関する実験的研究

The purpose of this study was to investigate the possibility of applying hydroxyapatite ceramics as endodontic-endosseous implants. The upper medial incisors of 3 monkeys (Macaca fuscatae) were used. To prepare the implant cavity, the teeth were extracted and drilled from the apex by steel burns under water-cooling. Then, dense hydroxyapatite implants, 10mm in length, 2mm in maximum diameter and 1/20 tapered, were inserted into the cavity to have the implant project 3-4mm above the apex. When the teeth with the implants were replanted, the bone around the apex was removed. The teeth were splinted to the neighbouring teeth for 1 month. Five months after the operation, the specimens were taken out and fixed by 10% formalin alcohol. They were embedded in polyester resin and undecalcified sections were prepared. The sections were stained with toluidine blue and observed under light microscope. 1) At 5 months, no ankylosis between the tooth and the surrounding alveolar bone was observed. 2) There was newly formed hard tissue which extended from the cementum, on the surface of the implant. 3) Fiber bundles were observed around the implants which connected the newly formed hard tissue to the surrounding bone tissue. The results suggest that the application of hydroxyapatite ceramics as an endodontic-endosseous implant is effective.