Denomination Of Origin Research Articles

High gamma-aminobutyric acid (GABA) producing Enterococcus malodoratus isolated from protected denomination of origin (PDO) cheese

Four strains of Enterococcus malodoratus were isolated from São Jorge cheese – and showed a high conversion of L-glutamate to GABA, achieving a yield above 50%. These new strains neither fermented lactose nor produced hydrogen sulphide. They produced slow acidification and low amounts of diacetyl, and lacked most enzymatic activities and genes encoding virulence traits. They were sensitive to ampicillin, vancomycin, fluoroquinolones, chloramphenicol and streptogramins, but resistant to penicillin, oxacillin, erytromycin and tetracycline. One strain (SJC62) showed antimicrobial activity against Listeria monocytogenes, Salmonella enterica, Escherichia coli and Pseudomonas aeruginosa; and antifungal activity against Aspergillus caesiellus and Mucor racemosus. The strains also showed probiotic potential in vitro, as they exhibited good resistance to gastrointestinal conditions, high hydrophobicity and mucin adhesion. The spectrum of favourable technological, antimicrobial and probiotic properties, in combination with their high ability to convert glutamic acid into GABA make these strains relevant for use in novel dairy foods.

Future aridity and drought risk for traditional and super-intensive olive orchards in Portugal

Portugal, a leading olive oil producer, boasts six Protected Denomination of Origin (PDO) regions, with distinct olive orchard (OR) densities (traditional rainfed to super-intensive irrigated). This study aimed to assess future drought and aridity conditions and the impacts on ORs located in the PDOs. Therefore, drought and aridity indicators were considered for the historical (ERA5: 1981–2000) and future periods (2041–2060; 2081–2100), and anthropogenic forcing scenarios (RCP4.5 and RCP8.5), using a 7-member ensemble of global climate models. From Spearman’s correlation analysis, Annual Mean Aridity (AIA) was selected as the most representative indicator of the climate conditions, to which the ORs were exposed. Readily Available Soil Water (RAW; mm) was considered to represent the available soil water reservoir for olive trees. Moreover, the Olive Drought and Aridity Risk Index (ODAR) was developed to determine each OR's future risks. This index considered that the AIA and RAW were weighted by OR density fractions. In the future, southern Portugal will be more arid (0.69) than northern and central (0.60). ORs soil shows lower RAW in southern PDOs (< 60 mm) than in central and northern regions (> 90 mm). These results suggest that the south of ORs will be more exposed to water stress than the northern regions. According to ODAR, the ORs exposed to low and high risk will be mainly located in the central parts of the PDOs. In northern ORs, moderate to high risk will predominate. In the south, however, the risk will be very high, which means that the olive tree growth, fruit development, and olive oil quality could be negatively affected. Implementation of tailored adaptation measures will be required to improve the climate resiliency of the sector.

Effects of Aging in Wood Casks on Anthocyanins Compositions, Volatile Compounds, Colorimetric Properties, and Sensory Profile of Jerez Vinegars

The Jerez (Sherry) vinegars, including Vinagre de Jerez, Reserva, and Gran Reserva, are crafted from Sherry wines and are protected under the Denomination of Origin in Spain. The aim of this study was to (i) characterize the physicochemical properties and composition; (ii) investigate the impact of the aging process on color properties, phenolics, volatile compounds, and sensorial profiles; and (iii) find a marker for tracing the authenticity of non-aged (Apto) and aged Jerez vinegars. The phenolic components were identified through LC-MS/MS, whereas the volatile compounds were examined using the GC-MS/MS technique. As the aging progressed, a decrease was observed in the levels of flavonol and phenolic acids, with anthocyanin components being undetectable in non-aged and aged samples. In the Gran Reserva variety, 2-methylbutyl acetate, acetic acid, and ethanol emerged as the predominant volatile substances. The presence of oaklactone and ethyl butanoate components served as marker substances to authenticate the Gran Reserva. Additionally, alterations in color properties were noted, marked by a decrease in yellow content and an increase in the red component depending on aging. Furthermore, novel sensory descriptors, such as vanilla, clove, woody, and nutty notes, and winy character emerged in the samples with prolonged aging.

Determining Carbohydrates for Increasing Safety: GC-FID Quantification of Lactose, Galactose, Glucose, Tagatose and Myo-Inositol in ‘Maturo’ PDO Pecorino Sardo Cheese

Although PDO Pecorino Sardo is one of the oldest traditional cheeses of Sardinia, Italy, data on its nutritional properties and food safety are lacking. In particular, significant amounts of lactose and galactose may be a health concern for consumers. The primary objective of this study is to quantify, using a validated GC-FID method, the residual lactose and galactose content in “maturo” (i.e., ripened for at least two months) Protected Denomination of Origin (PDO) Pecorino Sardo cheese. A statistically representative sampling from seven dairies distributed throughout Sardinia has been selected for this aim. In addition to lactose and galactose, two of their metabolites (i.e., glucose and tagatose, respectively) and a bioactive polyol like myo-inositol were quantified. The concentration of lactose (mean 26 mg kg−1, range 4–90 mg kg−1) was below the strictest limit set in the European Union (i.e., 100 mg kg−1), while the galactose content was found to be in an amount (mean: 76 mg kg−1, range: 10–200 mg kg−1) that even patients afflicted with severe galactosemia, albeit with some circumspection, could consume this cheese. Ripening (two to four months) had no significant effect on the amount of all analytes, while a slight decrease in galactose levels was observed during the manufacturing season. Finally, the amounts of glucose, tagatose, and myo-inositol are constant in the range of a few tens of mg kg−1.

Decision-making model in ancestral knowledge management: The case of the Raicilla in Mexico

Ancestral knowledge is essential in the construction of learning to preserve the sense of relevance, transmit and share knowledge according to its cultural context, and maintain a harmonious relationship with nature and sustainability. The objective of this research is to study and analyze the management of ancestral knowledge in the production of the Raicilla to provide elements to rural communities, producers, and facilitators in decision-making to be able to innovate and be more productive, competitive, sustainable, and improve people’s quality of life. The methodological strategy was carried out through Bayesian networks and Fuzzy Logic. To this end, a model was developed to identify and quantify the critical factors that impact optimally managed technology to generate value that translates into innovation and competitive advantages. The evidence shows that the optimal and non-optimal management of knowledge, technology, and innovation management and its factors, through the causality of the variables, permits us to capture the interrelationship more adequately and manage them. The results show that the most relevant factors for adequate management of ancestral knowledge in the Raicilla sector are facilitators, denomination of origin, extraction and fermentation, and government. The proposed model will support these small producers and help them preserve their identity, culture, and customs, contributing greatly to environmental sustainability.

Disentangling the relationships between denomination of origin regulatory councils activities and Spanish wineries' export performance

AbstractWorld markets for quality differentiated agri‐food products are highly competitive, presenting significant challenges for firms aiming to compete effectively. Government agencies and business organizations often implement various export promotion policies to address these challenges. This paper explores the role of Denomination of Origin Regulatory Councils in the export activities of Spanish wineries. Hypotheses are developed based on the Resource‐Based View and Institutional‐Based View, considering divergent theoretical predictions. Empirical findings from a comprehensive sample of Spanish wineries across all Denominations of Origin reveal positive associations between wineries' resources and Regulatory Councils' activities with export performance. However, these relationships vary depending on the specific resource or activity. These findings are consistent across different measures of export performance, highlighting the intricate relationships between institutions like Regulatory Councils and the internationalization of member firms.

Geographical origin determination of the PDO hazelnut (cv. Giresun Tombul) by chemometric analysis of FT-NIR and Raman spectra acquired from shell and kernel.

Turkey is the leading producer of hazelnuts, contributing to 62% of the total global production. Among 18 distinct local hazelnut cultivars, Giresun Tombul is the only cultivar that has received Protected Designation of Origin denomination from the European Comission (EC). However, there is currently no practical objective method to ensure its geographic origin. Therefore, in this study NIR and Raman spectroscopy, along with chemometric methods, such as principal component analysis, PLS-DA (partial least squares-discriminant analysis), and SVM-C (support vector machine-classification), were used to determine the geographical origin of the Giresun Tombul hazelnut cultivar. For this purpose, samples from unique 118 orchards were collected from eight different regions in Turkey during the 2021 and 2022 growing seasons. NIR and Raman spectra were obtained from both the shell and kernel of each sample. The results indicated that hazelnut samples exhibited distinct grouping tendencies based on growing season regardless of the spectroscopic technique and sample type (shell or kernel). Spectral information obtained from hazelnut shells demonstrated higher discriminative power concerning geographical origin compared to that obtained from hazelnut kernels. The PLS-DA models utilizing FT-NIR (Fourier transform near-infrared) and Raman spectra for hazelnut shells achieved validation accuracies of 81.7% and 88.3%, respectively, while SVM-C models yielded accuracies of 90.9% and 86.3%. It was concluded that the lignocellulosic composition of hazelnut shells, indicative of their geographic origin, can be accurately assessed using FT-NIR and Raman spectroscopy, providing a nondestructive, rapid, and user-friendly method for identifying the geographical origin of Giresun Tombul hazelnuts. PRACTICAL APPLICATION: The proposed spectroscopic methods offer a rapid and nondestructive means for hazelnut value chain actors to verify the geographic origin of Giresun Tombul hazelnuts. This could definitely enhance consumer trust by ensuring product authenticity and potentially help in preventing fraud within the hazelnut market. In addition, these methods can also be used as a reference for future studies targeting the authentication of other shelled nuts.

What happened to the subjects? Questions about mezcal

Mezcal is a Mexican agave spirit that has helped to create a sense of authenticity among global consumers, significantly increasing its presence on the world market. This has led to a great expansion of the production areas included in the Denomination of Origin, changing its artisanal forms of production and creating an ambiguous position for the subjects that produce this drink. The aim of this essay is to contribute a new theoretical perspective to the academic debate on the patrimonialisation of mezcal, incorporating analyses that focus on the issues behind the production of this emblematic beverage. It discusses the need to understand the productive dynamics of this predominantly rural beverage, based on an understanding of the relationships between subjects at different levels of the production chain. We have opted for a classical theoretical framework that dares to consider mezcal producers as peasants and that affirms the explanatory potential of revising the classics of peasant social theory in order to offer new insights into the problem of dispossession through the appropriation of the biocultural knowledge that underlies the production of mezcal.

International property rights for Cannabis landraces and terroir products. The case of Moroccan Cannabis and hashish

BackgroundIn recent years, the cannabis industry has evolved from a world defined by the simplicity and ubiquity of illegality of recreational drug cannabis to a world marked by the legal and geographic complexity of ongoing depenalisation, decriminalisation, and legalisation processes. Within this landscape where drug Cannabis plants and their many derivatives see their legal status change, Cannabis cultigens and end products are increasingly likely to becoming subject to protection by intellectual property rights. This article delves into the implications of these changes for traditional Cannabis farmers, particularly in the Global South, as they face economic and legal threats amidst global legalisation efforts. It examines the potential role of appellations of origin in protecting local Cannabis cultigens and end products, focusing on Moroccan Cannabis and hashish as a case study. MethodsThe text resorts to the treaties and agreements regulating international property rights and plant variety protection, but also to the concepts of terroir and landrace and their definitions, in order to determine, by way of treaty interpretation and conceptual analysis, what type of legal and economic protections can apply to Cannabis landraces and terroir products. The analysis is also based on previous empirical research published by the author. ResultsThe text argues that appellations of origin are the best intellectual property protections possible for landraces and terroir products because what needs to be protected is not innovation and individual ownership, but tradition and collective ownership, and because appellations of origin are suitable collective intellectual property rights. It shows that appellations of origin are best suited to protect terroir products and landraces because their originality and distinctiveness are place-based originality and distinctiveness. ConclusionThe text concludes that appellations or origin offer the only existing intellectual property protection for preserving the distinctiveness of terroir cannabis products, and for landrace conservation. It acknowledges that neither appellations of origin nor existing plant variety protection laws can be legal forms of control of third parties’ uses of landraces but that appellations of origin can help protect terroir products and landraces by way of their associated agro-ecosystems.

Toward the authentication of wines of Itata valley denomination of origin through total reflection x-ray fluorescence (TXRF) method for testing adulteration. A novel valuable tool by using Compton/Rayleigh scattering signals in wines

The evaluation of wine adulteration is often a cumbersome process not exempt from multiple difficulties. In this work, a valuable methodology to rapidly assess wine adulteration was used. Harnessing the ratio of Compton and Rayleigh scattering signals obtained in TXRF (total reflection X-ray fluorescence). The Compton/Rayleigh signal ratio as a sensitive way to estimate the average effective atomic number (Zeff) of a sample was used. Thus, any addition made into the wine, would cause a change in its Zeff which could be detected by TXRF. Non-adulterated and adulterated wines were selected and its Zeff was estimated. The method was developed using X-ray excitation Molybdenum tube. Deconvolution of independent Compton and Rayleigh signals was performed by non-Gaussian and Gaussian curve resolution methods, and the area ratio was evaluated. A calibration curve for Compton/Rayleigh signal ratio versus Zeff was established and wine adulterated samples were tested in a Zeff range between 4.3 and 7.2. Wine adulteration was detected in all cases. The method is simple, fast, sensitive, precise and non-destructive. It procedure is usefulness as an important tool for wine industry and for the maintenance of origin and quality of wines.

Generation of tourism from the Denomination of Origin (D.O.) “Raicilla case”

Generation of tourism from the Denomination of Origin (D.O.) “Raicilla case”

Discrimination of Brazilian green canephora coffee beans by ultraviolet–visible spectroscopy as a non-target analysis: A tool for recognizing geographical indications

Specialty green coffee beans have a higher commercial value and some of them have recently been classified in Brazil based on the indication of provenance and denomination of origin. In this context, the classification of the type of coffee bean is still a challenge, using traditional analytical techniques. Thus, alternative analytical techniques, such as ultraviolet–visible spectroscopy (UV–Vis), as non-target analysis can be applied as a quick and reliable method of coffee classification using data science, such as chemometric tools. In the present study, UV–Vis were evaluated as a new strategy for discrimination of green beans of Brazilian specialty canephora coffees with recognized geographical indications (Robusta Amazônico and Conilon from state of Espírito Santo), for the first time. Spectra obtained from the aqueous extract of 222 samples. The Principal Component Analysis (PCA) was performed and subsequently Partial Least Squares with Discriminant Analysis (PLS-DA) model developed. The PCA indicated tendency to group the samples in their respective classes, pointing to the similarities in the spectra of samples of the same origin. The PLS-DA model obtained showed figures of merit values starting at 89.3% in the test set. The VIP scores showed that the variables associated with chlorogenic acids, caffeine and chlorophyll are the most important for differentiating the studied coffees. The results obtained showed that UV–Vis fingerprint − non-targeted analysis associated with PLS-DA is appropriate for the discrimination of green beans of Brazilian specialty coffee from different origins, in a simple way, using common equipment in several laboratories.

The Portuguese mertolenga cattle breed: a review.

This review summarizes the state of the art regarding the phylogenetic origins, recent history and present-day main traits and uses of the Mertolenga cattle breed from Southern Portugal, particularly those related to production performances and product quality. Named after the historical city of Mértola, in southern Portugal, the Mertolenga is one of the fifteen autochthonous bovine breeds of Portugal. It is a cattle breed thoroughly adapted to the poor Mediterranean pastures of the southern regions of the Iberian Peninsula. It is used predominantly in an extensive to semi-intensive sylvopastoral production system called montado, where pastures are combined with helm and cork trees. Its productive traits allow for a good adaptation to the intense dry heat and pasture shortage during the summer, and compensatory growth in autumn and spring, when pastures regenerate. They are small to medium sized animals, with well-balanced bodies, roan, red, or, less often, red-spotted coats, and known for their nervous temperament. Although this breed experienced a severe decline in numbers in the 1970s and classified as endangered in the 1990s, the work of a few breeders led to the establishment of larger Mertolenga breed inventories, starting from a limited base. For this reason, the entire breed has today a strong influence from a very few herds and sires. Reproduction is still mostly achieved using natural mating, and the males are often kept with the breeding females all year long. It is a heterogeneous breed both phenotypically and genetically. Recent phylogenetic studies have revealed the Mertolenga as a one of the most genetically diverse breeds in the country and in the Iberian Peninsula and helped classify this breed, once believed to be a variety of the Alentejana breed. These studies also showed genetic relations with other breeds in the Iberian Peninsula. Mertolenga beef currently benefits from several certifications, the most important one being the PDO - Protected Denomination of Origin.

Participatory ecodesign of crop management based on Life Cycle Assessment: an approach to inform the strategy of a Protected Denomination of Origin. A case study in viticulture

Since the context on environmental issues is challenging, the Cognac protected denomination of origin (PDO) adopted life cycle assessment (LCA) to inform its environmental strategy, initially focusing on vineyard soil management. This study developed a participatory eco-design approach involving stakeholders to inform the PDOs’ strategy for transitioning to less impactful crop management practices. It did this by drawing on literature that emphasises the use of participatory design, serious games, and LCA to foster knowledge sharing, engagement, and sustainability assessment. The approach that was put to the test in the Cognac PDO involved 17 elected officials with varying levels of experience. The participants used the serious game known as Vitigame (a game done for participatory ecodesign in viticulture) to ecodesign a soil management and fertilisation pathway for 2030, with the process guided by the results of the LCA. Exchange dynamics, LCA results, and participant feedback were analysed. The study revealed diverse dynamics and efficient ecodesign strategies among the participant groups, which resulted in a reduction of environmental impacts by up to 51% and raised new questions for the territory. The diversity of participants presented challenges, highlighting the need to improve LCA knowledge sharing. The workshop’s results could inform the PDO’s strategic planning. The interest of this innovative process including a serious game was confirmed, suggesting potential applications to other viticultural PDOs and adaptability to diverse sectors.

Diversity of Culturable Yeasts Associated with the Technification Level in the Process of Mezcal Production in the State of Durango

Durango State has the denomination of origin for the production of mezcal, which is made from Agave durangensis, mainly in an artisanal way; therefore, differences in the fermentation process affect the quality of the final product. The main objective of the present study was to evaluate the diversity of culturable yeasts involved in the artisanal and semi-technified process of mezcal production in the State of Durango. Three distilleries with different production processes were monitored at different fermentation stages (beginning, mid-fermentation, and end of fermentation) in the spring and summer seasons. A greater diversity was found in the distillery of Nombre de Dios in both the spring and summer production seasons (H’ = 1.464 and 1.332, respectively), since it maintains an artisanal production process. In contrast, the distillery of Durango, where a Saccharomyces cerevisiae commercial inoculum is used to start fermentation, presented low diversity indexes (H’ = 0.7903 and 0.6442) and only S. cerevisiae, Kluyveromyces marxianus, and, sporadically, Pichia manshurica were found. Results suggest that the yeast microbiota involved in mezcal fermentation during the different seasons is affected by the type of inoculum; changes include the presence of some species that were only identified during a specific season in alcoholic fermentation, such as Torulaspora delbrueckii and Pichia kluyveri.

Geographical indications in brazilian regions: a strategy for protection and local development

Geographical Indication (GI) refers to a certification granted for products or services that present unique characteristics or that originate from territories that are renowned as centers of extraction or production, favoring local development and the appreciation of traditions, customs and knowledge. This research aimed to identify in Brazilian territory the regions that invest most in the search for GI as a strategy for protection and local development. Therefore, searches were carried out on the website of the National Institute of Industrial Property (INPI) to identify GIs recognized in Brazil in the modalities of Indication of Origin (IP) and Denomination of Origin (DO). Subsequently, two scripts were prepared for data collection, considering the GI modality (IP or DO), type of certified product or service, year of concession, region (North, Northeast, South, Southeast, and Central-West), and state. The main results found indicate that there are several areas demarcated by GI in Brazil, especially in the IP modality. In addition, the use of GI as a protection and local development strategy is more significant in the Southeast and South regions, when compared to other regions. Therefore, it is necessary to form collaboration networks between the different social actors to act in the dissemination of knowledge and in the structuring of new GI requests and the demarcation of new areas.

TaqMan® and HRM approaches for SNP genotyping in genetic traceability of musts and wines

The fight against fraud in the wine sector requires continuous improvements and validations of new technologies applicable to musts and wines. Starting from published data from the Vitis18kSNP array, a series of new specific single nucleotide polymorphism (SNP) markers have been identified for some important north-western Italian cultivars, such as Barbera, Dolcetto and Arneis (Vitis vinifera L.), used in the production of high-quality wines under Protected Denomination of Origin. A pair of new SNP markers for each grape variety were selected and validated using two real-time PCR techniques: TaqMan® genotyping assays and high-resolution melting analysis (HRM). The TaqMan® assay has proven to be more reliable and repeatable than HRM analysis because despite being an economical and versatile technique for the detection of different types of genomic mutations (SNPs, insertions or deletions), HRM has shown limitations in the presence of poor-quality DNA extracted from musts and wines. TaqMan® assays have successfully identified Barbera, Dolcetto and Arneis in their respective musts and experimental wines, and with good efficiency in commercial wines. Marked differences between genotypes were observed, varietal identification in Dolcetto-based musts/wines was more efficient than that in Arneis-based wines. Therefore, the TaqMan® assay has considerable potential for varietal identification in wines and the procedure described in the present work can be easily adapted to all wines with adequate setup of DNA extraction methods that should be adapted to different wines.

Vanilla production in the world and Mexico: Market value and technology

Vanilla and vanillin are essential commodities for the food, pharmaceuticals, cosmetics, and tobacco industries. However, the global production of natural vanillin is insufficient to meet its demand and has been replaced by synthetic or biotechnological substitutes. Consequently, the opportunity to promote this sector is being missed, and the introduction of natural products with higher added value into the market is not encouraged. Therefore, this study sought, first, to conduct a study about the production and value of vanilla cultivation in Mexico and the world, and second, to examine the technology sectors that have developed around the production and processing of vanilla and vanillin. The methodological development includes: a) an export behavior study and a three-year forecast; b) a factor analysis to build a crop production index; c) the identification of conglomerates to define groups according to their behavior; and d) patent and network analyses to show the main technological sectors, countries, and applicants of technologies relevant to our object of study. Results show that vanilla production is dominated by countries such as Madagascar and Indonesia; although Mexico presents positive results in terms of yield, its participation is limited, in spite of having the appellation of origin for this crop. Data on Mexico indicate that technological development is being carried out by foreign companies and is oriented toward the industrial use of vanillin, disregarding agricultural development. The literature and results point to a lack of a public policy in México to promote this sector and insufficient scientific and technological development to achieve sustainable production. Training and market plans for the benefit of small producers should also be facilitated to boost their participation in the international arena.

Trademarks and Denomination of Origin in Portuguese Wine Sector (1918-1924)

In the Portuguese wine sector there was a strong adhesion of companies, producers and traders to the registration of trade marks since the modern commercial brands emerged after the creation of the legal framework for their protection, at the end of the 19th century. We also must consider the existence of different regions of production, from north to south of the country, and the historical process of recognition of the different denomination of origin, which occurred since the beginning of the 20th century, in a context of post-phylloxera reconversion, market instability and the proliferation of imitations and counterfeits. Because wine sector was strategic in the Portuguese economy, in this article we will analyze the marks of different companies, traders and producers representative of various Portuguese wine regions, between 1918 and 1924 from the Trademark Registration in the Boletim de Propriedade Industrial (Industrial Property Bulletin). The aim is to understand the relationship between trademarks and appellations of origin, to understand if there was the growth of the different terroirs and their types of wines, and to identify, on a semiotic basis, representative and identifying elements of the indication of provenance, used in countless brands. Keywords: Portuguese wine regions, brands, trademarks, labels, semiotics

Appellation of Origin and Geographical Indication: Comparative Legal Aspect

The article provides a comparative legal analysis of the concepts of appellation of origin and geographical indication. Often there is an identification of these concepts, in connection with which it seems necessary to correlate these designations and conduct their comparative characteristics in accordance with the norms of international agreements and the legislation of the Russian Federation. The norms of international agreements in the field of protection of the appellation of origin and geographical indication are analyzed. Examples from judicial practice are given. Examples of the appellation of origin of goods and geographical indication known in the Russian Federation are considered. As examples of the appellation of origin of goods, such goods as “Krasnodar tea”, “Vologda butter”, “Tula gingerbread”, “Astrakhan watermelons”, “Zhostovo”, “Khokhloma”, “Gzhel”, “Altai honey”, “Astrakhanskaya sturgeon caviar”. Well-known examples of geographical indications are cheese “Roquefort”, “Shuyskoye soap”.