Denitrification Kinetics Research Articles

Greenhouse gas emission and denitrification kinetics of woodchip bioreactors treating onsite wastewater

The accurate evaluation of denitrification rate and greenhouse gas (GHG) emission in field-scale woodchip bioreactors for onsite wastewater treatment are problematic due to inevitably varied environmental conditions and underestimated GHG production with limited analysis of dissolved gas in field samples. To address these problems, batch incubation experiments were conducted with controlled conditions to precisely evaluate the denitrification kinetics and N2O and CH4 emission of both gaseous and dissolved phases in fresh (6 months) and aged (5 years) woodchip bioreactors treating onsite wastewater at high (1–3 mg L-1) and no (0 mg L-1) dissolved oxygen (DO) levels. NO3- removal rate decreased from 37.5–119.0 g NO3--N m-3d-1 at no DO to 8.8–16.6 g NO3--N m-3d-1 at high DO (1–3 mg L-1) due to the growth suppression of NO2- reducing microorganisms (37–55 % lower nirS+nirK abundance). However, the presence of high DO increased N2O emission level from 5.6–6.9 mg N2ON m-3 at no DO to 179.5–273.6 mg N2ON m-3) due to the enhanced growth of NO reducing microorganisms (1–7 times higher norB levels) and the decreased abundance of N2O reducing microorganisms (53–75 % lower nosZ abundance). On the other hand, increased DO level negatively correlated with CH4 production (1.0–3.9 g CH4-C m-3d-1) in fresh woodchips, while showed insignificant impact on CH4 production (0.1–1.4 g CH4-C m-3d-1) in aged woodchips. Woodchip age increase (5 years) negatively impacted the NO3- removal rate (75–85 % lower than fresh woodchips) and CH4 production rate (>3 times lower than fresh woodchips), probably due to the reduced biomass density of NO2- reducing microorganisms (52–58 % lower nirS+nirK abundance) and methanogens (95–98 % lower mcrA levels). The incubation results suggested that long hydraulic retention time (>2–5 days) and anaerobic/anoxic condition are preferred for the optimal NO3- removal and low N2O emission potential of woodchip bioreactors treating onsite wastewater.

Characterisation of Pseudomonas mosselii H6 for aerobic denitrification: stoichiometry and reaction kinetics

The application of aerobic denitrifying bacteria in biological nitrogen removal has been of great interest. However, the mechanism of nitrogen conversion by aerobic denitrifying bacteria is unclear due to the lack of stoichiometry and kinetic studies. Therefore, this study aimed to investigate the stoichiometry and kinetics of aerobic denitrification using Pseudomonas mosselii H6 as a model strain. The results showed that the stoichiometric equations of strain H6 under different nitrogen source conditions were obtained by nitrogen balance analysis; the concentrations of different limiting substrates were set and combined with the multi-substrate Monod kinetic equation to fit the half-saturation constants of COD, ammonia, nitrate and nitrite nitrogen for strain H6, which were 230.1008, 25.3435, 4.1009 and 23.4601 mg/L, respectively; based on the model of “two-step denitrification”, the numerical simulation of the growth and aerobic denitrification of strain H6 under different limiting substrate concentrations was carried out by using AQUASIM software to test the validity of stoichiometric coefficients and kinetic constants and perfect fitting results were obtained. These parameters will help to simulate the growth and denitrification performance of aerobic denitrifying bacteria and provide an experimental basis for further quantitatively elucidating the mechanism of action in the aerobic denitrification process.

Unraveling the impact of perfluorooctanoic acid on sulfur-based autotrophic denitrification process

PFOA has garnered heightened scrutiny for its impact on denitrification, especially given its frequent detection in secondary effluent discharged from wastewater treatment plants. However, it is still unclear what potential risk PFOA release poses to a typical advanced treatment process, especially the sulfur-based autotrophic denitrification (SAD) process. In this study, different PFOA concentration were tested to explore their impact on denitrification kinetics and microbial dynamic responses of the SAD process. The results showed that an increase PFOA concentration from 0 to 1000 μg/L resulted in a decrease in nitrate removal rate from 9.52 to 7.73 mg-N/L·h. At the same time, it increased nitrite accumulation and N2O emission by 6.11 and 2.03 times, respectively. The inhibitory effect of PFOA on nitrate and nitrite reductase activity in the SAD process was linked to the observed fluctuations in nitrate and nitrite levels. It is noteworthy that nitrite reductase was more vulnerable to the influence of PFOA than nitrate reductase. Furthermore, PFOA showed a significant impact on gene expression and microbial community. Metabolic function prediction revealed a notable decrease in nitrogen metabolism and an increase in sulfur metabolism under PFOA exposure. This study highlights that PFOA has a considerable inhibitory effect on SAD performance.

The superiority of citrate as denitrification carbon source in an activated sludge system: Substrate metabolism, electron transfer and microbial characteristics

Citrate, a low-molecular-weight organic substance, was rich in citric acid production wastewater with the potential for reuse. Although citrate has been extensively used as a denitrification carbon source, previous studies mainly focus on its application by pure cultures of denitrifying bacteria. The information about denitrification performance and mechanisms using citrates as carbon source in an activated sludge system is still very limited. This study investigated the denitrification efficiency and mechanisms using citrate as carbon source through a long-term operation of continuous reactor. Under the C/N ratio of 5, the total nitrogen (TN) removal efficiency using citrate as carbon source was 93.21 ± 4.24 %, which was comparable to that of acetate (99.84 ± 0.44 %), and much higher than glucose (65.64 ± 3.86 %). Higher denitrification rate was achieved using citrate as carbon source, which was slightly higher than acetate and >4 times of that using glucose. Due to the high activities of denitrifying enzymes including nitrite reductase (NIR), nitric oxide reductase (NOR) and nitrous oxide reductase (N2OR), there was almost no NO2‐-N and N2O accumulation in the reactor fed with citrate, which was superior to the reactors fed with acetate or glucose. Azoarcus (48.2 %) with the ability of complete denitrification was the most dominant bacteria in the reactor fed with citrate. Considering denitrification kinetics, activities of key enzymes and microbial community characteristics, citrate had a superiority as an effective denitrification carbon source.

New insights into the effect of EDTA on pyrite oxidation and N2O emission during pyrite autotrophic denitrification

Pyrite autotrophic denitrification (PAD) is crucial for removing nitrate in groundwater and advanced wastewater treatment. While disulfide is typically seen as the electron donor, the role of iron in pyrite oxidation is often ignored. In this study, Ethylene Diamine Tetraacetic Acid (EDTA) had been proven to have the potential to facilitate pyrite oxidation by promoting the solubility of iron in solution, thereby enhancing the kinetics of pyrite autotrophic denitrification. Firstly, EDTA could improve PAD performance, i.e. the nitrate removal rate at EDTA addition of 5 mM was 0.69 mg N/L•h and twice that of the control. However, high concentrations of EDTA inhibited the activity of the nitrous oxide reductase, resulting in the maximum accumulation of N2O reaching 13.26 mg N/L with an EDTA concentration of 15 mM. Furthermore, the X-ray Photoelectron Spectroscopy (XPS) result indicated that the EDTA addition promoted the production of bioavailable sulfur, with the concentration of S0 at 15 mM EDTA being more than twice higher than that of the control. Transmission electron microscopy (TEM) analysis indicated that EDTA could relieve the damage of iron encrustation on cell activity because it was difficult for EDTA-chelated ferrous iron to penetrate the cell membrane. Microbial community analyses showed an increase abundance of iron oxidizing bacteria, suggesting that EDTA addition promoted the bioavailability of iron for Fe(II) autotrophic denitrification. Overall, this study will provide insights into the understanding of oxidation mechanism of PAD process and the practical application for efficient nitrogen removal and N2O mitigation.

Insights into the transport and bio-degradation of dissolved inorganic nitrogen in the biochar-pyrite amended stormwater biofilter using dynamic modeling

The stormwater biofilter is a prevailing green infrastructure for urban stormwater management, but it is less effective in dissolved nitrogen removal, especially for nitrate. The mechanism that governs the nitrate leaching and performance stability of stormwater biofilters is poorly understood. In this study, a water quality model was developed to predict the ammonium and nitrate dynamics in a biochar-pyrite amended stormwater biofilter. The transport of dissolved nitrogen species was described by advection-dispersion models. The kinetics of adsorption and pyrite-based autotrophic denitrification are included in the model and simulated with a steady-state saturated flow. The model was calibrated and validated using eleven storm events. The modeling results reveal that the contribution of pyrite-based autotrophic denitrification to nitrate leaching alleviation improves with the increased drying duration. The nitrate removal efficiency was affected by a series of design parameters. Pyrite filling rate has a minor effect on nitrate removal promotion. Service area ratio and submerged zone depth are the key parameters to prevent nitrate leaching, as they influence the emergence and discharge time of nitrate breakthrough. The high inflow volume (high service area ratio) and small submerged zone can lead to earlier and increased discharge of peak nitrate otherwise the peak nitrate could be retained in the submerged zone and denitrified during the drying period. The developed mechanistic model provides a useful tool to evaluate the treatment ability of stormwater biofilters under varying conditions and offers a guideline for biofilter design optimization.

Factors Affecting Nitrous Oxide Emissions from Activated Sludge Wastewater Treatment Plants—A Review

Nitrous oxide (N2O) is a greenhouse gas contributing to ozone layer depletion and climate change. Wastewater treatment plants (WWTPs) contribute significantly to the global anthropogenic N2O emissions. The main factors affecting N2O emissions are the dissolved oxygen concentration (DO), the nitrite accumulation, the rapidly changing process conditions, the substrate composition and COD/N ratio, the pH, and the temperature. Low DO in the nitrification process results in higher N2O emissions, whereas high aeration rate in the nitration/anammox process results in higher N2O production. High DO in the denitrification inhibits the N2O reductase synthesis/activity, leading to N2O accumulation. High nitrite accumulation in both the nitrification and denitrification processes leads to high N2O emissions. Transient DO changes and rapid shifts in pH result in high N2O production. Ammonia shock loads leads to incomplete nitrification, resulting in NO2− accumulation and N2O formation. Limiting the biodegradable substrate hinders complete denitrification, leading to high N2O production. A COD/N ratio above 4 results in 20–30% of the nitrogen load being N2O emissions. Maximum N2O production at low pH (pH = 6) was observed during nitrification/denitrification and at high pH (pH = 8) during partial nitrification. High temperature enhances the denitrification kinetics but produces more Ν2O emissions.

Climate change effects on denitrification performance of woodchip bioreactors treating agricultural tile drainage

Denitrifying woodchip bioreactors (WBRs) are a nature-based technology that are increasingly used to control nonpoint source nitrate (NO3−) pollution in agricultural catchments. The treatment effectiveness of WBRs depends on temperature and hydraulic retention time (HRT), both of which are affected by climate change. Warmer temperatures will increase microbial denitrification rates, but the extent to which the resulting benefits to treatment performance may be offset by intensified precipitation and shorter HRTs is not clear. Here, we use three years of monitoring data from a WBR in Central New York State to train an integrated hydrologic-biokinetic model describing links among temperature, precipitation, bioreactor discharge, denitrification kinetics, and NO3− removal efficiencies. Effects of climate warming are assessed by first training a stochastic weather generator with eleven years of weather data from our field site, and then adjusting the distribution of precipitation intensities according to the Clausius-Clapeyron relationship between water vapor and temperature. Modeling results indicate, in our system, faster denitrification rates will outweigh the influence of intensified precipitation and discharge under warming, leading to net improvements in NO3− load reductions. Median cumulative NO3− load reductions at our study site from May - October are projected to increase from 21.7% (interquartile range 17.4%–26.1%) under baseline hydro-climate to 41.0% (interquartile range 32.6–47.1%) with a + 4 °C change in mean air temperature. This improved performance under climate warming is driven by strong nonlinear dependence of NO3− removal rates on temperature. Temperature sensitivity may increase with woodchip age and lead to stronger temperature-response in systems like this one with a highly aged woodchip matrix. While the impacts of hydro-climatic change on WBR performance will depend on site-specific properties, this hydrologic-biokinetic modeling approach provides a framework for assessing climate impacts on the effectiveness of WBRs and other denitrifying nature-based systems.

Effect of copper, arsenic and nickel on pyrite-based autotrophic denitrification

Pyritic minerals generally occur in nature together with other trace metals as impurities, that can be released during the ore oxidation. To investigate the role of such impurities, the presence of copper (Cu(II)), arsenic (As(III)) and nickel (Ni(II)) during pyrite mediated autotrophic denitrification has been explored in this study at 30 °C with a specialized microbial community of denitrifiers as inoculum. The three metal(loid)s were supplemented at an initial concentration of 2, 5, and 7.5 ppm and only Cu(II) had an inhibitory effect on the autotrophic denitrification. The presence of As(III) and Ni(II) enhanced the nitrate removal efficiency with autotrophic denitrification rates between 3.3 [7.5 ppm As(III)] and 1.6 [7.5 ppm Ni(II)] times faster than the experiment without any metal(loid) supplementation. The Cu(II) batches, instead, decreased the denitrification kinetics with 16, 40 and 28% compared to the no-metal(loid) control for the 2, 5 and 7.5 ppm incubations, respectively. The kinetic study revealed that autotrophic denitrification with pyrite as electron donor, also with Cu(II) and Ni(II) additions, fits better a zero-order model, while the As(III) incubation followed first-order kinetic. The investigation of the extracellular polymeric substances content and composition showed more abundance of proteins, fulvic and humic acids in the metal(loid) exposed biomass.Graphical

Development of a mathematical model for a microbial denitrification co-culture system comprising acetogenic bacterium Sporomusa ovata and denitrifying bacterium Pseudomonas stutzeri.

Previous study has shown that co-culturing acetogenic bacterium Sporomusa ovata (SO), with denitrifying bacterium Pseudomonas stutzeri (PS), is a promising strategy to enhance the microbial denitrification for nitrate-contaminated groundwater remediation. However, the mutual effects and reaction kinetics of these two bacteria in the co-culture system are poorly understood. In this study, a mathematical model for this co-culture system was established to fill this knowledge gap. Model simulation demonstrated that SO had a significant effect on the kinetics of denitrification by PS, while PS slightly affected the kinetics of acetate production by SO. The optimal initial HCO3-/NO3- ratio and SO/PS inoculation ratio were 0.77-1.48 and 67 for the co-culture system to achieve satisfied denitrification performance with less acetate accumulation. Finally, the minimum hydrogen supply was recommended when the initial bicarbonate and nitrate concentrations were assigned in the range of 2-20 mM and 2-4 mM for simulating the natural nitrate-contaminated groundwater treatment. These findings could provide useful insights to guide the operation and optimization of the denitrification co-culture system.

NaHS regenerated Fe(II) EDTA denitrification mechanism and kinetics investigation

Fe(II) EDTA regeneration is a hot research topic in wet denitrification. In this study, NaHS was used to regenerate Fe(II)EDTA. The effects of Fe(II)EDTA-NO concentration, temperature and pH on the reduction of NaHS were investigated separately. On this basis, the reduction reaction mechanism and kinetics are clarified. A comparison was made with other reductants in terms of reduction efficiency and cost. The experimental results show that the reduction of Fe(II)EDTA-NO by NaHS is a first-order reaction for Fe(II)EDTA-NO. The NaHS reduction rate increases with increasing temperature. The destination of N in the reduction reaction is N2 and the destination of S is mainly S monomer. In addition, the activation energy (Ea) for the reduction of Fe(II)EDTA-NO by NaHS was 34.328 kJmol-1, the enthalpy of activation (ΔH#) was31.7879 kJmol-1, and the entropy of activation (ΔS#) was −160.3614 Jk-1mol-1. In addition, NaHS can simultaneously reduce Fe(II)EDTA-NO and Fe(III)EDTA, and Fe(II)EDTA-NO will reduce the efficiency of NaHS reduction of Fe(III)EDTA. These data can further promote the industrial denitrification of Fe(II)EDTA.

Different responses of representative denitrifying bacterial strains to gatifloxacin exposure in simulated groundwater denitrification environment

The impact of antibiotics on denitrification in the ecological environment has attracted widespread attention. However, the concentration threshold and inhibitory effect of the same antibiotic on denitrification mediated by mixed denitrifying microbes were conflicting in some studies. In this study, Paracoccus denitrificans, Acidovorax sp., and Pseudomonas aeruginosa were selected as representative denitrifying bacterial strains to explore the response of a single strain to gatifloxacin (GAT) exposure in groundwater denitrification. The results showed that the nitrate and nitrite removal efficiencies of Pseudomonas aeruginosa decreased by 34.87–36.25 % and 18.27–23.31 %, respectively, with exposure to 10 μg/L GAT, accompanied by a significant decline in denitrifying enzyme activity and gene expression. In contrast, the elevated denitrifying enzyme activity and gene expression of Paracoccus denitrificans promoted its nitrate and nitrite reduction by 2.09–10.00 % and 0–8.44 %, respectively. Additionally, there were no obvious effects on the removal of nitrate and nitrite by Acidovorax sp. in the presence of 10 μg/L GAT, which was consistent with the variation in denitrifying enzyme activity and total gene expression levels. The fit results of the Monod equation and its modification further elucidated the nitrate degradation characteristics from the perspective of denitrification kinetics. Furthermore, antibiotic resistance gene (ARG) analysis showed that the addition of 10 μg/L GAT (approximately 30 days) did not observably increase the relative abundance of ARGs. This study provides some preliminary understanding of the response differences of representative denitrifying bacterial strains to antibiotic exposure in groundwater denitrification.

Denitrification kinetics during aquifer storage and recovery of drainage water from agricultural land

An aquifer storage transfer and recovery (ASTR) system was studied in which tile drainage water (TDW) was injected with relatively high NO3 (about 14 mg/L) concentrations originating from fertilizers. Here we present the evolution of denitrification kinetics at 6 different depths in the aquifer before, and during ASTR operation. First-order denitrification rate constants increased over time before and during the first days of ASTR operation, likely due to microbial adaptation of the native bacterial community and/or bioaugmentation of the aquifer by denitrifying bacteria present in injected TDW. Push-pull tests were performed in the native aquifer before ASTR operation. Obtained first-order denitrification rate constants were negligible (0.00–0.03 d−1) at the start, but increased to 0.17–0.83 d−1 after a lag-phase of about 6 days. During the first days of ASTR operation in autumn 2019, the arrival of injected TDW was studied at 2.5 m distance from the injection well. First-order denitrification rate constants increased again over time (maximum >1 d−1). Three storage periods without injection were monitored in winter 2019, fall 2020, and spring 2021 during ASTR operation. First-order rate constants ranged between 0.12 and 0.61 d−1. Denitrification coupled to pyrite oxidation occurred at all depths, but other oxidation processes were indicated as well. NO3 concentration trends resembled Monod kinetics but were fitted also to a first-order decay rate model to facilitate comparison. Rate constants during the storage periods were substantially lower than during injection, probably due to a reduction in the exchange rate between aquifer solid phases and injected water during the stagnant conditions. Denitrification rate constants deviated maximally a factor 5 over time and depth for all in-situ measurement approaches after the lag-phase. The combination of these in-situ approaches enabled to obtain more detailed insights in the evolution of denitrification kinetics during AS(T)R.

Nitrite accumulation, denitrification kinetic and microbial evolution in the partial denitrification process: The combined effects of carbon source and nitrate concentration

The combined effects of carbon source (HAc, HPr, Glu, Glu + HAc) and nitrate concentration (40, 80 mg/L labeling as R40, R80) on partial denitrification (PD) were discussed at C/N ratio of 2.5 (COD = 100, 200 mg/L). The optimal NO2−-N and NTR reached to 67.03 mg/L, 99.14% in HAc-R80 system, and denitrification kinetics revealed the same conclusion, corresponding to higher COD utilization rate (CUR: 58.46 mgCOD/(gVSS·h)), nitrate reduction rate (NaRR: 29.94 mgN/(gVSS·h)) and nitrite accumulation rate (NiAR: 29.68 mgN/(gVSS·h)). The preference order was HAc > HPr > Glu + HAc > Glu in both R40 and R80 systems due to different metabolic pathways, however, the NO2−-N accumulation and kinetic parameters of R80 group were dramatically higher than those in R40 for the same carbon source. The R80 group facilitated more concentrated biodiversity (607–808 OTUs) with Terrimonas and norank_f_Saprospiraceae responsible for high NO2−-N accumulation in HAc and HPr served systems, while norank_f_norank_o_Saccharimonadales and OLB13 dominated the Glu containing systems.

A new insight to explore toxic Cd(II) affecting denitrification: Reaction kinetic, electron behavior and microbial community

Denitrification process is a crucial step in nitrogen removal and is more vulnerable to external shocks due to the fact that anoxic process is always located before aerobic process in conventional sewage treatment. This study aims to elaborate the nitrogen conversion characteristics by investigating denitrification kinetics, electron behavior and microbial community under Cd(II) shock. Reaction kinetics showed that 10 mg/L of Cd(II) accelerated nitrate reduction rate by 52.29% but 80 mg/L of Cd(II) severely decelerated it by 95.41% with the accumulation of nitrite. High concentration of COD (C/N = 10.4) in the system caused by Cd(II) disrupting the integrity of cell membrane (lactate dehydrogenase increased by 328.7%) was proved to induce occurrence of Dissimilatory Nitrate Reduction to Ammonia (DNRA). The electron transport system activity (ETSA), electron consumption and electron distribution were combined to reveal the electron behavior regulated by Cd(II). The electron ratio of nitrate reductase to nitrite reductase increased from 1.48 (control) to 3.91 and 3.52 (40 and 80 mg/L of Cd(II)) indicated the electrons allocating tendency and further explained the nitrite accumulation. High concentration of Cd(II) also decreased ETSA by weakening the physiological activities of flavin adenine dinucleotide, flavin mononucleotide and cytochrome c or hindered the microbes to secrete these electron carriers. Furthermore, Cd(II) inhibited dominant bacteria genera containing napA gene (Azospirillum and Thauera) and nirS gene (unclassified_c_Betaproteobacteria). Enterobacteriaceae family was found to dominate the DNRA process.

N2-Based Determination of Denitrification Kinetics with Confirmation of Simultaneous Denitrification and Fermentation of Carbohydrates

N₂-Based Determination of Denitrification Kinetics with Confirmation of Simultaneous Denitrification and Fermentation of Carbohydrates

Nitrogen and Phosphorus Removal Efficiency and Denitrification Kinetics of Different Substrates in Constructed Wetland

Constructed wetlands (CWs) are generally used for wastewater treatment and removing nitrogen and phosphorus. However, the treatment efficiency of CWs is limited due to the poor performance of various substrates. To find appropriate substrates of CWs for micro-polluted water treatment, zeolite, quartz sand, bio-ceramsite, porous filter, and palygorskite self-assembled composite material (PSM) were used as filtering media to treat slightly polluted water with the aid of autotrophic denitrifying bacteria. PSM exhibited the most remarkable nitrogen and phosphorus removal performance among these substrates. The average removal efficiencies of ammonia nitrogen, total nitrogen, and total phosphorus of PSM were 66.4%, 58.1%, and 85%, respectively. First-order continuous stirred-tank reactor (first-order-CSTR) and Monod continuous stirred-tank reactor (Monod-CSTR) models were established to investigate the kinetic behavior of denitrification nitrogen removal processes using different substrates. Monod-CSTR model was proven to be an accurate model that could simulate nitrate nitrogen removal performance in vertical flow constructed wetland (VFCWs). Moreover, PSM demonstrated significant pollutant removal capacity with the kinetics coefficient of 2.0021 g/m2 d. Hence, PSM can be considered as a promising new type of substrate for micro-polluted wastewater treatment, and Monod-CSTR model can be employed to simulate denitrification processes.

Comparison of primary and secondary sludge carbon sources derived from hydrolysis or acidogenesis for nitrate reduction and denitrification kinetics: Organics utilization and microbial community shift

Seeking available and economical carbon sources for denitrification process is an intractable issue for wastewater treatment. However, no study compared different types of waste sludge as carbon source from denitrification mechanism, organics utilization and microbial community aspects. In this study, primary and secondary sludge were pretreated by thermophilic bacteria (TB), and its hydrolysis or acidogenic liquid were prepared as carbon sources for denitrification. At C/N of 8–3, the variations of NO3−-N and NO2−-N were profiled in typical cycles and denitrification kinetics was analyzed. Primary sludge achieved a competitive NOX-N removal efficiency with less dosage than secondary sludge. Fourier transform infrared (FTIR) spectroscopy was introduced to analyze organic composition from functional-group perspective and the utilization of organic matters in different sludge carbon sources was investigated. To further analyze the microbial community shift in different denitrification systems, high-throughput sequencing technology was applied. Results showed that denitrifier Thauera, belonging to Proteobacteria, was predominant, and primary sludge acidogenic liquid enriched Thauera most intensively with relative abundance of 47.3%.

Cornstalk biochar promoted the denitrification performance and cellulose degradation rate of Burkholderia sp. CF6

A strain Burkholderia sp. CF6 with denitrification and cellulose degradation ability was isolated. When sodium carboxymethyl cellulose (CMC-Na) dosage was 5 g L−1, the nitrate removal efficiency reached 96.48% after 48 h and the nitrite accumulation was only 0.07 mg L−1. The exponential decay equation was used to match the kinetics of denitrification and the correlation coefficient (R2) was higher than 0.940. The denitrification and cellulose degradation capabilities were promoted by cornstalk biochar. Through the comparison of nitrogen balance of different biochars, biochar of 400 ℃ (BC400) had the highest gaseous nitrogen conversion efficiency (83.31%). BC400 can accelerate the nitrate removal rate (from 0.08 to 0.32 mg L−1 h−1) and shorten the peak time of cellulase activity (from 48 to 24 h). The fourier transform infrared spectroscopy (FTIR) manifested that the BC400 assisted biological denitrification was achieved through phenolic and quinone groups. From the results of three-dimensional excitation-emission matrix (3D-EEM), it can be concluded that soluble microbial by-product and aromatic protein have a significant correlation with the effect of BC400 on the biological process of strain CF6. This research will provide new insights for agricultural waste utilization to treat low carbon to nitrogen (C/N) ratio wastewater.

How to Produce an Alternative Carbon Source for Denitrification by Treating and Drastically Reducing Biological Sewage Sludge.

Minimizing the biological sewage sludge (BSS) produced by wastewater treatment plants (WWTPs) represents an increasingly difficult challenge. With this goal, tests on a semi-full scale Thermophilic Alternate Membrane Biological Reactor (ThAlMBR) were carried out for 12 months. ThAlMBR was applied both on thickened (TBSS) and digested biological sewage sludge (DBSS) with alternating aeration conditions, and emerged: (i) high COD removal yields (up to 90%), (ii) a low specific sludge production (0.02–0.05 kgVS produced/kgCODremoved), (iii) the possibility of recovery the aqueous carbon residue (permeate) in denitrification processes, replacing purchased external carbon sources. Based on the respirometric tests, an excellent biological treatability of the permeate by the mesophilic biomass was observed and the denitrification kinetics reached with the diluted permeate ((4.0 mgN-NO3−/(gVSS h)) were found comparable to those of methanol (4.4 mgN-NO3−/(gVSS h)). Moreover, thanks to the similar results obtained on TBSS and DBSS, ThAlMBR proved to be compatible with diverse sludge line points, ensuring in both cases an important sludge minimization.