Dengue Prevention Research Articles

Identification of Primary Container of Aedes Mosquitoes Breeding Site in Urban Region of Dengue Endemic Area, Purwokerto Indonesia

Dengue prevention and control more reliant on reducing its vector, Aedes sp. mosquitoes by mosquito breeding nest eradication method. Therefore, identification of the primary container of the Aedes sp. breeding site particularly in the urban region of dengue-endemic area is a crucial effort to conduct an effective dengue prevention program. This research aimed to identify the primary container of the Aedes sp. breeding site in the urban region of the dengue-endemic area. This is a descriptive cross-sectional study, located in an urban region of dengue-endemic area within Banyumas Regency of Central Java Indonesia. A total of 300 houses in three urban areas were observed in urban areas of Purwokerto namely Arcawinangun, Purwanegara, and Karangpucung (100 houses each area). A total of 1504 water-holding containers were observed. All container was observed and recorded the type, color, lid condition, position, and the presence of mosquito larvae. The analysis was conducted by calculating the percentage of types, color, lid condition, and position of the container also the presence of mosquito larvae. The result of this study highlighted that flower pots and bathtubs were the primary types of containers with Aedes sp. larvae in the urban area. The characteristics of larvae-positive containers mostly open, bright, and located indoor position. Dengue prevention efforts by targeting the primary types of containers for mosquito breeding are expected to reduce the adult mosquito population.

A simplified monotone model of Wolbachia invasion encompassing Aedes aegypti mosquitoes

AbstractWolbachia‐based biocontrol has recently emerged as a potential method for the prevention and control of dengue and other vector‐borne diseases. Major vector species, such as Aedes aegypti females, when deliberately infected with Wolbachia become far less capable of getting infected and transmitting the virus to human individuals. In this paper, we propose and qualitatively analyze a simplified model of Wolbachia invasion that describes an interaction and competition between Wolbachia‐infected and wild Aedes aegypti mosquitoes. This model not only facilitates better visualization of the Wolbachia invasion dynamics thanks to its bidimensionality, but it also possesses the noticeable property of monotonicity and exhibits the saddle‐point behavior. The latter helps to identify a threshold manifold consisting of points in that couple the minimum viable population sizes of wild and Wolbachia‐carrying insects and also regulate the survival and extinction of each population of mosquitoes.

Knowledge About Dengue Fever Prevention Among People Visiting Benazir Bhutto Hospital

Background: The higher incidence of dengue fever in Pakistan demands additional efforts in order to limit the disease. Despite active public health campaigns, low public awareness is one of the factors facilitating dengue virus transmission. For effective preventive measures, the assessment of the knowledge gap and then taking appropriate steps to fill the gap is required.The objective of this study is to assess knowledge about dengue fever prevention among people visiting Benazir Bhutto Hospital, Rawalpindi.Methods: The descriptive cross-sectional study of 6 months duration was conducted with 280 participants selected via nonprobability convenience sampling. After informed consent, an interview was conducted based on a questionnaire that assessed socio-demographic parameters and knowledge about dengue virus transmission and prevention. Data were analyzed through SPSS v. 22. The study was approved by the Ethical Review Board (ERB) of Rawalpindi Medical University and Allied hospitals.Results: Out of 280 respondents, 54.6% were males and 45.4% females and the mean age was 35.0 ± 13.1 years. The respondents having high knowledge scores were 66(23.6%) while those having moderate and low scores were 159 (56.8%) and 55 (19.6%) respectively. Educated respondents (p=0.03) and urban residents (p=0.05) had higher knowledge scores.Conclusion: The majority of the participants know about dengue fever. However, only one out of every four respondents has good knowledge scores for dengue fever prevention.

Participation of Community in Prevention and Control of Dengue Fever in Dharan Sub Metropolitan City of Province No 1, Nepal

Background: Dengue fever is known as a break-bone fever and its common symptoms are headaches, high grade fever, muscular, bone pains and decrease of platelets count. Community participation involving local government and household participants in controlling breeding areas of dengue mosquitoes is the only cost-effective and sustainable activities of ensuring prevention and control of Dengue fever. This study aimed to assess the perception of the general population regarding community participation in the prevention of dengue and to verify its effectiveness with the control practices observed at the homes in Dharan sub-metropolitan city of Province No 1.
 Material and Methods: A cross-sectional study was conducted among 250 households at the Dharan sub-metropolitan city of Province no. 1 in Nepal from August to October 2019. The technique of sample collection was done using purposive sampling and data were collected using a pretested close-ended questionnaire and observational checklists.
 Results: Slightly over half of the respondents 129 (51.6%) reported a positive attitude regarding community practice and almost three out of five 157 (62.8%) houses were observed to have good dengue control practices. The univariate results revealed attitude of households regarding community participation were significantly associated with the control practices observed in their homes (p<0.001).
 Conclusion: The study concludes that good community participation in collaboration with health volunteers and health institutions can have a positive impact on the dengue control practices at the household level. Hence, the spread of dengue out-break can be controlled by active collaborative community participation.

Health preparedness plan to prevent dengue epidemic in an urban area of Delhi

Background: Dengue is a vector-borne disease that has emerged as an important public health threat. Since the mid-1990s, outbreaks of dengue in India have become more frequent, especially in urban zones and the trend continues to rise. The number of dengue cases has increased 30-fold globally over the past five decades. Therefore, this study aims to find out the knowledge and preparedness of individuals against dengue. Methods and materials: It was a community based cross-sectional study conducted in urban area of Delhi for a period of 3 months. 200 adults were selected by house-to-house visit using systematic random sampling. A pre-tested predesigned questionnaire in local language was used to take information about socio demographic profile and knowledge and preparedness of individuals regarding dengue. Data was analysed using SPSS software. Chi-square/fisher exact or T test/Mann Whitney tests were used to find any significance. Institutional ethical clearance was taken for the study and written informed consent was taken from all participants. Results: Knowledge regarding transmission of dengue was correct in 160 (80%) subjects. Knowledge regarding type of mosquit that causes dengue was adequate in only 12.5% of the subjects. Only 9% of the subjects had good knowledge regarding symptoms of dengue. Similarly only 8% had good knowledge regarding prevention of dengue. Prevention practices were good in 27.5% subjects. Only 7 participants had adequate knowledge score regarding prevention of breeding of mosquitoes both inside and outside the house. Only twenty three percent of the subjects had received health promotion by one or more means. No significant association was seen between good knowledge and gender, marital status or literacy status. Conclusion: Knowledge and practices were not satisfactory towards dengue fever. Additional training is required to plug this gap. People need to be educated regarding symptoms of dengue fever and measures of prevention of dengue fever.

Knowledge, attitude and practices about dengue fever among adults living in Pwani Region, Tanzania in 2019.

Dengue fever (DF) is currently widespread in tropical and sub-tropical countries. Among the triggers of epidemic include urbanization and internal migrations. Within the past few years, there have been DF outbreaks in Tanzania. Although Pwani region is among the predicted risk areas for the DF, there is insufficient data about people's knowledge, attitude and practices towards prevention of DF in their settings. Therefore, the aim of this study was to assess knowledge, attitude and practices about DF among adults in Pwani region in Tanzania. The cross-sectional study conducted in Mkuranga District, Pwani region in Tanzania. We used face-to-face interviews to collect data. The main analytical procedure was descriptive using frequencies. The majority, 97.7%, were aware of DF. Nevertheless, almost 80% had a low knowledge on symptoms, transmission and vector control measures. Furthermore, less than 20% had positive attitude towards dengue fever prevention, severity of the illness and health seeking behavior. Lack of enough knowledge and positive attitude about disease transmission, symptoms and preventive measures put the population at high risk of contracting the disease. There is need to create and improve friendly, correct and simple information, education and education messages for the rural populations.

Factors Correlated with Practices Regarding Care of Dengue Patients Among Nurses from 94 Primary Care Units in a High- Risk Province in Southern Thailand.

BackgroundIn Thailand, primary care units (PCUs) play a part in the health assessment, diagnosis, care, basic treatment and referral of patients suffering from dengue.MethodsIn Nakhon Si Thammarat province in Thailand, we (i) undertook assessment of the personal information, experience, knowledge, attitudes, preparation of equipment and use of medical supplies of nurses in PCUs with regard to dengue patient care (DPC); (ii) analyzed the factors and practices of nurses regarding DPC; (iii) explored nurses’ opinions regarding DPC in PCUs. A cross-sectional mixed methods of collecting data consisted of quantitative and qualitative methods. The study cohort was 94 nurses from 94 PCUs in an area of high risk of dengue outbreaks. The quantitative component involved questionnaires. The qualitative component consisted of nurses’ discussions in focus groups. Spearman’s test was employed to analyze quantitative data, and thematic analyses were employed for qualitative data.ResultsOf 94 nurses from 94 PCUs, 77.7% (n = 73) had a poor knowledge level of DPC and negative attitude, preparation of equipment was carried out by 60.6% (n = 58), and 1 to 6 aspects of DPC were at good practice level: 88.3% (n = 83), 87.2% (n = 82), 85.1% (n = 80), 50.0% (n = 47), 51.1% (n = 48) and 77.7% (n = 73). There was no significant correlation (i) between the knowledge, attitudes and practices with regard to DPC (P > 0.05); (ii) with practice guidelines for dengue prevention (P > 0.05). The quantitative results were related to the four main themes of the qualitative study.ConclusionThere was a few who had good knowledge and positive attitude levels among participants, and >90% of all nurses had good practices regarding DPC. PCU nurses need training and supporting equipment to carry out a DPC program.

Comparative Study of Dengue Prevention Measures in Malaysian Construction Site

Dengue cases have been a significant health threat to Malaysia for more than a century since the year 1902, and this situation has worsened every year. This paper covered the comparative study to analyse further the differences and similarities between countries such as Singapore regarding the existing guidelines and legislation implemented towards dengue prevention measures. The method of this study is using a depth literature review which adopted from various and related documents such as journals, reports, proceedings, and Government Act. A total of 20 published article systematic surveys were included, a complete of 28 relevant references were identified by database literature search. Thus, a comparative analysis was conducted to compare and contrast dengue preventive measures in Malaysia and Singapore, suffering from dengue cases. Therefore, a comparison of perspectives legislations between Singapore and Malaysia can identify the efficiency of dengue prevention and the effectiveness of enforcement, which available to be implemented in Malaysia. In conclusion, it is essential to improve the legislations and guidelines for strengthening dengue prevention in Malaysia. Indirectly, it can finally solve the dengue issue in this country. Therefore, the government, especially the Ministry of Health, needs to reconsider these preventive measures to curb dengue cases in Malaysia.

Effect of community-based intervention on awareness of dengue and its prevention among urban poor community in India: a systematic review approach

Owing to increased epidemic activity and difficulties in controlling the insect vector, dengue has become a major public health problem globally. The prevention and control of dengue rely mainly on vector control methods. A systematic review was conducted using four databases (PubMed, Cochrane, Google Scholar and Scopus) and a manual search of the reference lists of the identified studies. Data from included studies were extracted, analyzed. Applying all inclusion and exclusion criteria 23 articles was included. Further relevant articles using this keywords Aedes dengue breeding habits housing and community intervention were selected. Eight studies combined community participation programme with dengue control tools. Findings of the published literature indicate that at baseline, almost half the respondents did not know that dengue is serious but preventable, or that it is transmitted by mosquitoes. The analysis showed that dengue vector control is carried out by vertically structured programmes of national, state, and local administrative bodies through fogging and larval control, without any involvement of community-based organizations, and that vector control efforts were conducted in an isolated and irregular way. The most productive container types for Aedes pupae were cement tanks, drums, and discarded containers. Evidence that community-based dengue control programmes alone and in combination with other control activities can enhance the effectiveness of dengue control programmes is weak. This review demonstrates a paucity of reliable evidence for the effectiveness of any dengue vector control method. Standardized studies of higher quality to evaluate and compare methods must be prioritized to optimize cost-effective dengue prevention. Clear best practice guidelines for the methodology of entomological studies should be developed.

Surveillance of dengue virus in individual Aedes aegypti mosquitoes collected concurrently with suspected human cases in Tarlac City, Philippines

BackgroundVector control measures are critical for the prevention and reduction of dengue virus (DENV) transmission. Effective vector control is reliant not only on knowledge of mosquito abundance, but also on the timely and accurate detection of mosquito-borne infection. Mosquito-based virus surveillance programs typically rely on pool-based mosquito testing, although whether individual-based mosquito testing is a feasible alternative to this has not been widely studied. Applying an individual-based mosquito testing approach, we conducted a 1-month surveillance study of DENV in adult Aedes aegypti mosquitoes in homes of suspected dengue patients during the 2015 peak dengue season in Tarlac City, Philippines to more accurately assess the mosquito infection rate and identify the DENV serotypes and genotypes concurrently co-circulating in mosquitoes and patients there.MethodsWe performed a one-step multiplex real-time reverse transcription-polymerase chain reaction (RT-PCR) assay for the simultaneous detection and serotyping of DENV in patients and individual female Ae. aegypti mosquitoes. Additionally, we performed sequencing and phylogenetic analyses to further characterize the detected DENV serotypes in mosquitoes and patients at the genotype level.ResultsWe collected a total of 583 adult Ae. aegypti mosquitoes, of which we individually tested 359 female mosquitoes for the presence of DENV. Ten (2.8%) of the 359 female mosquitoes were positive for the presence of DENV. We detected DENV-1, DENV-2, and DENV-4 in the field-collected mosquitoes, which was consistent with the serotypes concurrently found in infected patients. Sequencing and phylogenetic analyses of the detected DENV serotypes based on the partial sequence of the evelope (E) gene revealed three genotypes concurrently present in the sampled mosquitoes and patients during the study period, namely DENV-1 genotype IV, DENV-2 Cosmopolitan genotype, and DENV-4 genotype II.ConclusionsWe demonstrated the utility of a one-step multiplex real-time RT-PCR assay for the individual-based DENV surveillance of mosquitoes. Our findings reinforce the importance of detecting and monitoring virus activity in local mosquito populations, which are critical for dengue prevention and control.

Knowledge, attitudes, and practices on climate change and dengue in Lao People's Democratic Republic and Thailand

BackgroundDengue is linked with climate change in tropical and sub-tropical countries including the Lao People's Democratic Republic (Laos) and Thailand. Knowledge about these issues and preventive measures can affect the incidence and outbreak risk of dengue. Therefore, the present study was conducted to determine the knowledge, attitudes, and practices (KAP) among urban and rural communities and government officials about climate change and dengue in Laos and Thailand. MethodsA cross-sectional KAP survey about climate change and dengue were conducted in 360 households in Laos (180 urban and 180 rural), 359 households in Thailand (179 urban and 180 rural), and 20 government officials (10 in each country) using structured questionnaires. Data analysis was undertaken using descriptive methods, principal component analysis (PCA), Chi-square test or Fisher's exact test (as appropriate), and logistic regression. ResultsSignificant differences among the selected communities in both countries were found in terms of household participant's age, level of education, socioeconomic status, attitude level of climate change and KAP level of dengue (P < 0.05; 95% CI). Overall, participants' KAP about climate change and dengue were low except the attitude level for dengue in both countries. The level of awareness among government officials regarding the climatic relationship with dengue was also low.In Lao households, participants’ knowledge about climate change and dengue was significantly associated with the level of education and socioeconomic status (SES) (P < 0.01). Their attitudes towards climate change and dengue were associated with educational level and internet use (P < 0.05). Householders' climate change related practices were associated with SES (P < 0.01) and dengue related practices were associated with educational level, SES, previous dengue experience and internet use (P < 0.01).In Thailand, participants' knowledge about climate change was associated with the level of education and SES (P < 0.01). Their attitudes towards climate change were associated with residence status (urban/rural) and internet use (P < 0.05); climate change related practices were associated with educational level and SES (P < 0.05). Dengue related knowledge of participants was associated with SES and previous dengue experience (P < 0.05); participants’ dengue related attitudes and practices were associated with educational level (P < 0.01). ConclusionThe findings call for urgently needed integrated awareness programs to increase KAP levels regarding climate change adaptation, mitigation and dengue prevention to improve the health and welfare of people in these two countries, and similar dengue-endemic countries.

AWARENESS, KNOWLEDGE AND ATTITUDE ABOUT DENGUE AMONG SOCIETY

Objective: The survey about dengue was carried out to gather the information about dengue fever, create awareness among the people. To determined the index of knowledge, attitude and awareness among the surveyed population about dengue.&#x0D; Methods: The questionnaire was prepared under the guidance of a pharmacologist. Pilot study was conducted among ten nos. of the population prior actual study. Questionnaire was revised and finalized according to an input of the pilot study. This questionnaire presented to the hundred peoples of different age group. Method involves face to face interview and seeking answers for different questions. Responses were recorded for further analysis and interpretation. Responses were sorted and analyzed. Different questions were categorized into the domain of awareness, knowledge and attitude. Data was presented in the form of graphs.&#x0D; Results: Percentage of awareness, knowledge and attitude found to be 59, 44, 52 respectively. It is observed that three parameters are almost in the range of fifty percent. Precautions plays vital role in prevention of Dengue.&#x0D; Conclusion: it is concluded that there is scope for improvement for creating awareness, knowledge and attitude among the surveyed population as representative of the general population. This may help to overcome the risk of this communicable and deadly disease.

Lessons to Be Learnt For Policy Planners on Promoting Health Education on Childhood Fever Management: Evidence from a Cohort of Children with Dengue Fever

Introduction Dengue fever has been a public health challenge in Sri Lanka. Many of the home-based secondary preventive measures are influenced by the parental knowledge on fever. This study was done to describe the level of knowledge on fever among parents and its associations with the events of child’s dengue episode with the view of exploring the components that should be covered in related health educational messages. Methods A descriptive cross-sectional study was done in six general medical wards of the premier children’s hospital in Sri Lanka. Parents who were the bystanders of children admitted as suspected cases of dengue according to the surveillance guidelines, consisted the study population. The sample size was determined with a statistical formula. An interviewer administered questionnaire was used. Data collection was done by two trained data collectors. Descriptive statistics and the chi square test were used with a significance level of 5%. Prior ethics approval was obtained. Results Study sample included 423 parents with a response rate of 99.5%. Most were parents of children of school going age (64.8%) and majority were mothers (97.6%). In more than half (50.4%), the level of knowledge was unsatisfactory. The level of knowledge was significantly associated with the education level (p&lt;0.05), but not with the gender or the employment status (p&gt;0.05) of the parents. A higher knowledge was further associated with a higher utilization of thermometer, and the administration of the correct anti-pyretic dosage and early laboratory diagnosis (p&lt;0.05). Conclusions and Recommendations A higher level of knowledge on fever is associated with favourable clinical events in the dengue episode. The level of knowledge on fever must be improved irrespective of the gender and the employment status of the parents prioritizing those with a lower level of education. Public health interventions must be implemented by regular updating of public health staff’s knowledge on prevention of dengue, fever management their implications on laboratory diagnosis and treatment costs through in service training and in empowering the field health staff in disseminating relevant health education messages to the community.

Risk Factors associated with Dengue Fever Outbreak in Dire Dawa Administration City, Ethiopia, October, 2015

Background: Dengue Fever is under recognized mosquito borne viral disease prevalent in tropical and subtropical regions. In 2013, Ethiopia reported the first Dengue outbreak in Dire Dawa city which affected 11,409 people. On October 3/2015, five suspected serum samples from Dire Dawa were confirmed positive for Dengue at national laboratory. We investigated to determine associated risk factors and implement control measures. Methods: We conducted a 1:2 un-matched case control study from October 7-15/2015. Case was any person with fever of 2-7 days and more than two symptoms: headache, arthralgia, myalgia, rash, or bleeding from any part of the body. We recruited participants using purposive sampling from health facilities in Diredawa and used structured questionnaire to collect data. Multivariable logistic regression analysis was conducted to identify risk factors. Sixty-nine serum-samples were tested by Enzyme-Linked Immunosorbent Assay (ELISA). Results: We enrolled 210 participants (70 cases and 140 controls) in the study. Females accounted for 51.4% of cases and 57.1% of controls. The mean age was 23.7±9.5 standard deviation (SD) for cases and 31.2±13 SD for controls. Close contact with Dengue patient (Adjusted odds ratio (AOR) =5.36, 95% confidence interval (CI): 2.75-10.44), nonuse of bed-nets (AOR=2.74, 95% CI: 1.06-7.08) and stagnant water around the village (AOR=3.61, 95% CI: 1.31-9.93) were independent risk factors. From the samples tested, 42 were confirmed positive.Conclusions: Individuals who live with Dengue Fever patient, around stagnant water and do not use bed nets are at high risk of contracting the disease. Health education on Dengue prevention was given and mosquito breeding sites were drained. Strong vector prevention strategies are recommended by enhancing the existing malaria prevention and control program.Key Words: Dengue Fever, Dire Dawa, Outbreak, Risk factors.

Utility of entomological indices for predicting transmission of dengue virus: secondary analysis of data from the Camino Verde trial in Mexico and Nicaragua

BackgroundDengue vector entomological indices are widely used to monitor vector density and disease control activities. But the value of these indices as predictors of dengue infection is not established. We used data from the impact assessment of a trial of community mobilization for dengue prevention (Camino Verde) to examine the associations between vector indices and evidence of dengue infection and their value for predicting dengue infection levels. In 150 clusters in Mexico and Nicaragua, two entomological surveys, three months apart, allowed calculation of the mean Container Index, Breteau index, Pupae per Household Index, and Pupae per Container Index across the two surveys. We measured recent dengue virus infection in children, indicated by a doubling of dengue antibodies in paired saliva samples over the three-month period. We examined the associations between each of the vector indices and evidence of dengue infection at household level and at cluster level, accounting for trial intervention status. To examine the predictive value for dengue infection, we constructed receiver operating characteristic (ROC) curves at household and cluster level, considering the four vector indices as continuous variables, and calculated the positive and negative likelihood ratios for different levels of the indices. None of the vector indices was associated with recent dengue infection at household level. The Breteau Index was associated with recent infection at cluster level (Odds ratio 1.36, 95% confidence interval 1.14–1.61). The ROC curve confirmed the weak predictive value for dengue infection of the Breteau Index at cluster level. Other indices showed no predictive value. Conventional vector indices were not useful in predicting dengue infection in Mexico and Nicaragua. The findings are compatible with the idea of sources of infection outside the household which were tackled by community action in the Camino Verde trial.

Empowering Village Health Sanitation and Nutrition Committee members on dengue prevention and control: An educational interventional study in rural areas of Mysuru, Karnataka

Objectives: Dengue, among all the vector-borne diseases, continues to be a major public health problem in India. Dengue once considered being problem in urban areas, now it is increasingly found in rural areas. Thus, empowering the village level functionaries like members of Village Health Sanitation and Nutrition Committee (VHSNC) can help in prevention and control of dengue in the rural areas. The present study was conducted to assess the effectiveness of educational intervention on perception regarding dengue and its prevention among VHSNC members. Material and Methods: This prospective interventional study was conducted among all 305 VHSNC members of two randomly selected primary health centers of Mysuru talukas for the period of 6 months. Baseline levels of perception on dengue were collected using a pre-tested structured questionnaire. Educational intervention on various domains of dengue and its prevention was provided using audiovisual aids, handouts, and group discussion. Endline survey was conducted 1 month after the education sessions to assess the effectiveness intervention. Results: A total of 305 VHSNC members participated in the study. In pre-test survey, only 189 (61.9%) had ever heard of disease dengue. In post-test survey, 274 (91.3%) had heard of dengue. There was a statistically significant improvement in perception regarding, preventable nature of dengue, mode of transmission, breeding and biting habits of mosquito, source reduction measures, and personal protective measures against mosquito bites following educational intervention. Conclusion: Educational intervention was found to be effective in empowering village level stake holders like VHSNC members regarding dengue and its prevention.

Bead-based multiplex detection of dengue biomarkers in a portable imaging device.

Dengue is one of the most rapidly spreading mosquito-borne viral diseases in the world. Differential diagnosis is a crucial step for the management of the disease and its epidemiology. Point-of-care testing of blood-borne dengue biomarkers provides an advantageous approach in many health care settings, and the ability to follow more than one biomarker at once could significantly improve the management of the disease. Bead-based multiplex technologies (suspension array) can measure multiple biomarker targets simultaneously by using recognition molecules immobilized on microsphere beads. The overarching objective of our work is to develop a portable detection device for the simultaneous measurement of multiple biomarkers important in dengue diagnosis, monitoring and treatment. Here, we present a bead-based assay for the detection of one of the four serotypes of dengue virus non-structural protein (DENV-NS1) as well as its cognate human IgG. In this system, the fluorescent microspheres containing the classification fluorophore and detection fluorophore are imaged through a microfluidic chip using an infinity-corrected microscope system. Calibration curves were plotted for median fluorescence intensity against known concentrations of DENV-NS1 protein and anti-NS1 human IgG. The limit of quantitation was 7.8 ng/mL and 15.6 ng/mL, respectively. The results of this study demonstrate the feasibility of the multiplex detection of dengue biomarkers and present its analytical performance parameters. The proposed imaging device holds potential for point-of-care testing of biomarkers on a highly portable system, and it may facilitate the diagnosis and prevention of dengue as well as other infectious diseases.

The Study of Erigeron Canadensis in the treatment of Dengue

From centuries there are many species of plants and animals which have been discovered and are used in the treatment of some or the other disease for serving mankind. Similar to this another specie of a plant was also discovered and was used to treat more than one kind of disease named Erigeron Canadensis. It was found that it is affect full in treating a disease named dengue (CDC). It is a plant whose parts grow above the ground usually used for medicines. People use it for many medication purposes but there is no good scientific evidence to support these uses. It was predicted that it may be useful in dengue prevention used in mosquito repellents or for other factors but still there is n’t enough information known about Erigeron canadensis to know how it might work. The use of Erigeron, Canadensis is mostly in urban or local areas worldwide as it is one of the prevention method from dengue.

Dengue awareness, preventive behaviours and Aedes breeding opportunities among slums and slum-like pockets in Delhi, India: a formative assessment.

India is endemic for dengue. The present formative study reports awareness and practices of people regarding dengue and its prevention, personal protection behaviours and peoples' exposure to dengue-related health promotion activities. This is a cross-sectional study of 1194 households randomly selected from 20 slum clusters in Delhi. Data were collected from an adult member of the household through an interviewer-administered, pre-tested questionnaire. An observation checklist was used to identify potential breeding sources. People were aware that dengue is transmitted through mosquitoes and sources of breeding (clean, stagnant water -60.1%, domestic water containers -18.7%, coolers -15.0%). Each house had at least one potential source of mosquito breeding. Using mosquito repellents (83.3%) was the most common personal protection behaviour. Peoples' participation is limited in the mosquito prevention and health promotion activities carried out by the local municipality. Still, participation resulted in positive behavioural change. While people were aware of dengue transmission, preventive measures against mosquito breeding were not satisfactory. People could not relate their existing knowledge on dengue to their household surroundings, and their awareness had not been translated to behavioural change. Community participation is limited, but is significant in the prevention and control of dengue.

Cross-sectional study of dengue-related knowledge, attitudes and practices in Villa El Salvador, Lima, Peru

ObjectivesTo describe and quantify the dengue-related knowledge, attitudes and practices of residents in an urban shantytown in Lima, Peru.Design/settingA cross-sectional survey of adults between 18 and 80 years living in...