Degree Of Neutralization Research Articles

Characterization of anti-AAV2 neutralizing antibody levels in sheep prior to and following intravitreal AAV2.7m8 injection

Gene augmentation therapy is a promising treatment for incurable, blinding inherited retinal diseases, and intravitreal delivery is being studied as a safe alternative to subretinal injections. Adeno-Associated Viruses (AAV) are commonly-used vectors for ocular gene augmentation therapy. Naturally occurring pre-operative exposure and infection with AAV could result in presence of neutralizing antibodies (NAB’s) in patients’ serum, and may affect the safety and efficacy of treatment. Our aim was to characterize the humoral response against AAV pre- and post-intravitreal delivery of AAV2.7m8 vectors in a naturally-occurring sheep model of CNGA3 achromatopsia. Serial serum neutralization assays were performed to screen sheep for pre-exiting anti-AAV2 NAB’s, and to assess the effect of intravitreal AAV2.7m8 injection on post-operative NAB titers and intraocular inflammation in sheep. The effect of viral dose and transgene type were also assessed. Serological screening revealed pre-operative seropositivity in 21.4% of animals, with age being a risk factor for the presence of anti-AAV2 NAB’s. NAB titers increased following intravitreal AAV administration in the majority of sheep. There was no significant difference in the degree of post-operative serum neutralization between pre-operatively seronegative sheep and those with pre-existing antibodies. However, only sheep with pre-existing antibodies presented with signs of post-operative inflammation. We conclude that pre-existing anti-AAV2 NAB’s do not affect the level of post-operative NAB titers; however, they increase the risk of post-operative ocular inflammation. Our results could have implications for the management of AAV-mediated ocular gene therapies, a technology being increasingly studied and used in patients.

Hamacher Operations on Interval Valued Picture Fuzzy Matrices

Picture Fuzzy Sets (PFS) extend fuzzy sets and intuitionistic fuzzy sets, offering a more robust framework for handling ambiguous, uncertain, and imprecise data. Interval-valued Picture Fuzzy Set represent membership, neutral, and non-membership degrees as intervals, providing a flexible solution for situations where precise values are difficult to determine. This paper introduces novel Hamacher operations for interval-valued picture fuzzy matrices, building upon existing Hamacher operations for Picture Fuzzy matrices. We develop and examine scalar multiplication and exponentiation operations using these new Hamacher operations. This research advances the development of sophisticated medical diagnosis systems, leading to improved patient outcomes and more accurate decision-making.

On the Size Dependence in the Recent Time-Dependent Theory of Tropical Cyclone Intensification

Abstract Previous observational studies have shown that the intensification rate (IR) of a tropical cyclone (TC) is often correlated with its real-time size. However, no any size parameter explicitly appears in the recent time-dependent theory of TC intensification, while the theory can still well capture the intensity evolution of simulated TCs. This study provides a detailed analysis to address how TC real-time size affects its intensification and why no size parameter explicitly appears in the theory based on the results from axisymmetric numerical simulations. The results show that the overall correlation between the TC IR and real-time size as reported in previous observational studies, in terms of both the radius of maximum wind (RMW) and the radius of 17 m s−1 wind (R17), is largely related to the correlation between the IR and intensity because the size and intensity are highly interrelated. As a result, the correlation between the TC IR and size for a given intensity is rather weak. Diagnostic analysis shows that the TC real-time size (RMW and R17) has two opposing effects on intensification. A larger TC size tends to result in a higher steady-state intensity but reduce the conversion efficiency of thermodynamic energy to inner-core kinetic energy or the degree of moist neutrality of the eyewall ascent for a given intensity. The former is favorable, while the latter is unfavorable for intensification. The two effects are implicitly included in the theory and largely offset, resulting in the weak dependence of the IR on TC size for a given intensity.

The translation of food in Hong Lou Meng: a cross-linguistic study based on the English and French versions

ABSTRACT This paper presents the results of a corpus-based investigation of the English and French translations of Chinese food-related culture-specific items (CSIs) in the classic Chinese novel Hong Lou Meng (A Dream of Red Mansions). The aim of the study is threefold: i) to determine what (translation) techniques were commonly used to render these Chinese CSIs through systematic quantitative analysis; ii) to determine whether the translations tended to be foreignized, neutralized, or domesticated; and iii) to compare the two target texts (TTs) in order to identify similarities and differences. The quantitative analysis was conducted within the existing theoretical framework, and methods from the field of corpus-based studies were used. The results indicate two significant similarities between the English and French TTs: first, neutralizing techniques are the most prominent, followed by foreignizing and domesticating techniques in descending order; second, direct translation and paraphrase are the most frequently used techniques. However, data from the two TTs show significant differences in the distribution of techniques, the degree of neutralization, and the frequency of several techniques (e.g. generalization and compression). Moreover, comparing the English and French translations of the same Chinese CSI reveals more cross-linguistic differences than similarities.

Enabling Si‐Dominant Anodes: Influence of Neutralization Degree of Polyacrylic Acid on Low‐Cost Micron‐Sized Silicon Anode in High‐Energy Li‐Ion Full Cell

AbstractMicron‐sized silicon is a promising low‐cost, abundant material to increase the energy density of lithium‐ion batteries. Nevertheless, significant volume change and therefore excessive solid electrolyte interphase (SEI) growth lead to fast capacity fading. In this work, polyacrylic acid (PAA) with different neutralization degrees is used for the fabrication of Si anodes for practical applications. The electrochemical performance in full pouch cells reveals that the increase in neutralization degree of PAA up to 70 % enhances the overall performance by improved electrode properties, higher first cycle efficiency (FCE up to 78.1 % at C/3) and better capacity retention (85.4 % after 150 cycles at 1 C) over cycling, while with even higher neutralization degrees (such as 80 %) the performance declines. Since proper mixing of the slurry is another important factor, we optimized the mixing procedure by increasing the solid content of the slurry, which has shown positive influence on the electrochemical performance and electrode properties. To summarize, this work shows full cell 1 C cycling until capacity retention of 85 % after 150 cycles with pure Si microparticle anodes for 70 % neutralized PAA as well as increased C‐rate performance up to 5 C. Post‐mortem, less degradation on electrode and particle level is observed.

Essential Oil Content, Composition and Free Radical Scavenging Activity from Different Plant Parts of Wild Sea Fennel (Crithmum maritimum L.) in Montenegro.

This study was conducted to determine the sea fennel essential oil (SFEO) yield, composition, and antioxidant activity of leaves, stem, inflorescences, and umbels from seeds of wild sea fennel (SF) (Crithmum maritimum L.) from the Montenegro coast. The chemical composition of isolated essential oil was determined by GC/MS and GC/FID analyses. The antioxidant activity was determined using the DPPH assay. The maximum SFEO yield was found in umbels with seeds (4.77 mL/100 g p.m.). The leaves contained less EO (0.52 mL/100 g p.m.) than immature inflorescence (0.83 mL/100 g p.m.) The minimum EO content was found in the stem (0.08%). Twenty components were isolated from SFEO leaves, twenty-four from inflorescence, thirty-four components from the stem, and twenty-one components from umbels with seeds. Limonene (62.4-72.0%), γ-terpinene (9.5-14.0%), α-pinene (1.4-5.8%), and sabinene (1-6.5%) were found to be the main components of the SFEO from monoterpene hydrocarbons as dominant grouped components (86% to 98.1%). SF plant parts showed differences in chemical profiles, especially in specific and low-represented ingredients. (E)-anethole (4.4%), fenchone (0.5%), and trans-carveol (0.2%) were present only in umbel with seeds, while the β-longipipene (0.5%), (E)-caryophyllene (0.5%), and (2E)-decenal (0.2%) were found only in the stems. The degree of DPPH radical neutralization increased with incubation time. The SFEO isolated from the stems showed stronger antioxidant activity during the incubation times of 20 and 40 min (EC50 value of 5.30 mg/mL and 5.04 mg/mL, respectively) in comparison to the SFEO isolated from the other plant parts. The lowest antioxidant activity was obtained with the SFEO leaves (155.25 mg/mL and 58.30 mg/mL, respectively). This study indicates that SFEO possesses significant antioxidant activities and is animportant component in the food and pharmaceutical industries. It is important to preserve the existing gene pool and biodiversity with rational use SF for the extraction of high-quality essential oils.

Developments and Issues in Renewable Ecofuels and Feedstocks

Ecofuels and their feedstock come in three main product classes: electrofuels (e-Fuels), biofuels, and non-biowaste-derived fuels. Ecofuels originate from non-fossil sources, derived from circular raw materials such as non-food organic waste, renewable hydrogen, and captured CO2 through a rapid process of carbon fixation. Proposed regulation drafts under discussion indicate that new fuels would need to reach a substantial degree of climate neutrality. The manufacture of all ecofuels, however, requires energy input to accomplish the conversion of the initial feedstock; their climate neutrality claims stem from the use of renewable electric energy and/or biomasses in the production process, but fossil fuels are still the main primary sources of global (and the EU’s) electric power, and most biofuels consumed in the EU transport mix are still crop-based, with potential conflicts with food and land use. Furthermore, entirely neglecting GHG emissions from renewable energy generation is scientifically debatable, as the impact of the energy (and the related GHG emissions) embedded in the materials used to build renewable energy facilities is small, but not nil. The paper reports ecofuel trends according to the above-mentioned originating technologies and discusses the issues related to their development.

Are Solar Active Regions Born with Neutralized Currents?

Solar active regions (ARs) are formed by the emergence of current-carrying magnetic flux tubes from below the photosphere. Although for an isolated flux tube the direct and return currents flowing along the tube should balance with each other, it remains controversial whether such a neutralization of currents is also maintained during the emergence process. Here we present a systematic survey of the degrees of the current neutralization in a large sample of flux-emerging ARs which appeared on the solar disk around the central meridian from 2010 to 2022. The vector magnetograms taken by Helioseismic and Magnetic Imager onboard Solar Dynamic Observatory are employed to calculate the distributions of the vertical current density at the photosphere. Focusing on the main phase of flux emergence, i.e., the phase in which the total unsigned magnetic flux is continuously increased, we statistically examined the ratios of direct to return currents in all the ARs. Such a large-sample statistical study suggests that most of the ARs were born with currents close to neutralization. The degree of current neutralization seems to be not affected by the active-region size, the active-region growing rate, and the total unsigned current. The only correlation of significance as found is that the stronger the magnetic field nonpotentiality is, the further the AR deviates from current neutrality, which supports previous event studies that eruption-productive ARs often have non-neutralized currents.

A flexible, soft superabsorbent polyester-cotton blended fabric in-situ coated by poly(sodium acrylate-co-acrylic acid)

A novel pre-synthesized polymer is fabricated with sodium polyacrylate and acrylic acid through free radical polymerization. Interestingly, the pre-synthesized polymer is further polymerized on the polyester-cotton fabric in the presence of glycerol as a cross-linking agent forming superabsorbent fabric. The composite functional fabrics is prepared by two steps, namely synthesis and finishing process. Owing to the vital role of the viscosity of pre-polymer for the superabsorbent property, the effect of monomer concentration, temperature, time, dosage of initiator and degree of neutralization on the viscosity of pre-polymer is thoroughly investigated. Besides, the effects of the degree of neutralization and dosage of polymerization solution as well as cross-linking agent on the water absorption of the composite and unfinished polyester-cotton blended fabrics is compared, respectively. After treatment, the water absorption capacity increases from 1 to 323.52 g/g in 1 h, reaching a maximum value of 417.53 g/g after 3.5 h. Besides, the water transportation obeys a Fickian diffusion mechanism. Furthermore, the composite fabric exhibits an optimum water retention capacity of up to 4 h. This speedy and super-water-absorbing material exhibits great potential in practical applications, such as in sanitary articles, wound dressings, and soil moisture.

Optimization of preparation conditions and performance of a new degradable soil water retaining agent

Using polyaspartic acid (PAsp) and bentonite (BT) as the main raw materials, a new type of degradable soil water retaining agent (PAsp-AA/BT) was synthesized by microwave radiation. The optimum synthesis conditions and comprehensive properties of PAsp-AA/BT were discussed and the structure and surface characteristics of PAspsp-AA/BT were characterized by FTIR, SEM, XRD and TGA in the paper. The results showed that the optimum synthesis conditions of PAsp-AA/BT were as follows: the dosages of polyaspartic acid (PAsp), bentonite (BT), initiator potassium persulfate, crosslinking agent N,N′-methylene bisacrylamide was 5, 3, 0.3, 0.03%, respectively, the neutralization degree of acrylic acid was 75%, and the microwave power was 490W. Under this condition, the absorption ratio of the synthesized PAspsp-AA/BT in deionized water and 0.9% NaCl solution was 953 and 164 g/g, respectively. The synthesized PAsp-AA/BT had a high water absorption rate, good water retention and repeated water absorption, and the degradation rate in soil within 30 days reached 32.75%, with good degradation effect. The analysis of SEM, FT-IR, XRD and TGA showed that: the surface of PAsp-AA/BT was rough and had obvious pore structure, which was conducive to the diffusion of water molecules; polyaspartic acid, bentonite and acrylic acid were polymerized; the cross-linking structure was formed between polyaspartic acid, bentonite and acrylic acid; the product of PASP-AA/BT had good thermal stability. This study provides a new soil water retaining agent, which is helpful for the better development of soil water retaining agent research.

Preparation and Properties of Attapulgite-Hydroxyethyl Cellulose Composite Poly (Acrylic Acid-co-2-acrylamide-2-methylpropanesulfonic Acid) Concrete Internal Curing Material

Attapulgite-hydroxyethyl cellulose-poly (acrylic acid-co-2-acrylamide-2-methylpropanesulfonic acid) (ATP-HEC-P(AA-co-AMPS)) in-concrete curing material was synthesized by aqueous solution polymerization using attapulgite (ATP) as an inorganic filler and hydroxyethyl cellulose (HEC) as a backbone. The effects of relevant factors such as ATP dosage, HEC dosage, degree of neutralization, initiator quality, and cross-linking agent quality on the water absorption characteristics of ATP-HEC-P (AA-co-AMPS) were investigated through expansion tests. The micro-morphology of ATP-HEC-P (AA-co-AMPS) was also comprehensively characterized using Fourier transform infrared spectroscopy, scanning electron microscopy, a thermal analysis, and other applicable means. The results showed that the prepared ATP-HEC-P (AA-co-AMPS) had a strong water absorption and water retention capacity, with a water absorption multiplicity of 382 g/g in deionized water and 21.55% water retention capacity after being placed at room temperature for 7 d in a bare environment. Additionally, ATP-HEC-P (AA-co-AMPS) showed good performance for absorbing liquids within the pH range of 7–12. The material’s thermal stability and mechanical properties were also significantly improved after the addition of ATP. The preparation cost is low, the process is simple, and the material meets the requirements for concrete curing materials.

Study on the plume self-neutralization of ionic liquid electrospray thruster based on median potential

Abstract Ionic liquid electrospray thruster (ILET) has the advantages of high specific impulse, precise thrust control, and low structural mass, which make it ideal for small satellites. The charged particles of ILET’s plume may lead to device charging or even damage, restricting its engineering applications. Thus, this paper examines the self-neutralization effectiveness of the ILET's plume under various emission conditions using particle-in-cell simulations. In order to accurately evaluate the self-neutralization effectiveness of the ILET’s plume, the median potential is explained in this paper and its reasonableness as the evaluation criterion for self-neutralization of the plume is verified. The working envelope for achieving self-neutralization of the ILET’s plume is determined by simulating the bipolar plume under various emission conditions. The results indicate that when the highest and lowest potentials are the same, the mean electric field strength between two points in space with a better degree of neutrality is 200% higher compared to points with a lesser degree of neutrality. The study determines the working envelope to realize self-neutralization of the ILET’s plume with an effectiveness of 70%. When the emission voltage of the anode thruster is fixed, the range of the cathode thruster’s voltage ranges from 108.36 V to 228.74 V. The asymmetry between the anode and cathode emissions of the ILET prototype significantly influences the operational range of the cathode thruster. Greater asymmetry leads to a narrower operating range for the ILET to achieve self-neutralization of the plume. This study serves as a guide for the ILET to achieve self-neutralization of the plume.

Study on Preparation and Humidity-Control Capabilities of Vermiculite/Poly(sodium Acrylate-acrylamide) Humidity Controlling Composite.

This paper focuses on the preparation and evaluation of a novel humidity-control material, vermiculite/(sodium polyacrylate(AA)-acrylamide(AM)), using inverse suspension polymerization. Acrylic acid and acrylamide were introduced into the interlayer of modified vermiculite during the polymerization process, leading to the formation of a strong association with the modified vermiculite. The addition of vermiculite increased the specific surface area and pore volume of the composites. To investigate the moisture absorption and desorption properties of the composites, an orthogonal experiment and single-factor experiment were conducted to analyze the impacts of vermiculite content, neutralization degree, and the mass ratio of AA to AM. According to the control experiment, the addition of vermiculite was found to enhance the pore structure and surface morphology of the composite material, surpassing both vermiculite and PAA-AM copolymer in terms of humidity control capacity and rate. The optimal preparation conditions were identified as follows: vermiculite mass fraction of 4 wt%, a neutralization degree of 90%, and mAA:mAM = 4:1. The moisture absorption rate and moisture release rate of the composite material prepared under these conditions are 1.285 g/g and 1.172 g/g. The humidity control process of the composite material is governed by pseudo second-order kinetics, which encompasses the complete adsorption process. These results indicate that the vermiculite/PAA-AM composite humidity control material has excellent humidity control performance and is a simple and efficient humidity control method.

Factors influencing the decision-making process in breast reconstruction from the perspective of reconstructive surgeons: A qualitative study involving Korean plastic surgeons

BackgroundLittle research has been conducted on factors influencing the decision-making process for immediate breast reconstruction (IBR) options from the perspective of reconstructive surgeons, despite its significant impact on doctor-patient communication and shared decision-making. This study aims to explore the multiple factors and the mechanisms by which they interact using a qualitative methodology. We also address potential barriers to shared decision-making in IBR. MethodsSemistructured interviews were conducted with a purposive sample of reconstructive surgeons. Thematic analysis was used to identify key influences on IBR decision-making process from the perspective of reconstructive surgeons. ResultsFour major themes were identified: 1. Patient clinical scenarios; 2. Nonclinical practice environments; 3. Reconstructive surgeon preferences; and 4. Patient consultation. Reconstructive surgeons demonstrated diverse approaches to patient clinical scenarios. High-volume centers were significantly influenced by nonclinical factors such as scheduling and operating room allocation systems. Reconstructive surgeons often had strong personal preferences for specific IBR options, shaped by their expertise, experience, and clinical environment. Based on the preliminary decision, surgeons provided information with varying degrees of neutrality. Patients varied in their knowledge and participation, resulting in variation in the final decision authority among surgeons. ConclusionsThis study highlights the need to address nonclinical environmental constraints to improve shared decision-making process in IBR. Surgeons should recognize power imbalances in the doctor-patient relationship and be aware of their biases.

Neutralization of Anthropogenic Acidic Particles by NH3 From Wildfire Over Tropical Peatland

AbstractAcidity is an essential characteristic of aerosol particles that influences chemical and physical properties. Aerosol particles in tropical Asia, such as Singapore, have been reported as highly acidic due to intense anthropogenic sulfur emissions. At the same time, the region has also been experiencing wildfire over tropical peatlands, which is recognized as an intense source of NH3. Here, we investigated the role of NH3 from wildfire on aerosol particles in Singapore by employing the aerosol mass spectrometric technique. The observation was conducted both during wildfire haze (2015 October and 2019 September–October) and non‐haze (2018 October and 2019 April) periods. The observation result demonstrated that inorganic ionic species in Singapore were neutralized by NH4+ during the haze periods. Namely, the degree of neutralization of aerosol particles (i.e., measured NH4+ concentration/predicted NH4+ concentration by assuming that NH4+ fully neutralized SO42−, NO3−, and Cl−) was lower than 0.77during the non‐haze periods. On the other hand, the corresponding values were higher than 0.93 during the haze periods. In addition, NO3− concentration during the daytime of the haze period in 2015 was higher than that in other observation periods. A thermodynamic model calculation suggested that the regime shifts from the “NH3 sensitive region” to the “NH3 and HNO3 sensitive region” or “HNO3 sensitive region” might have occurred during the haze period. In the future, continuous monitoring of both gas‐ and particle‐phase inorganic chemical species will need to be conducted to investigate the impact of wildfire haze on atmospheric chemical processes in more detail.

A nonautonomous model for the effect of rarity value on the dynamics of a predator–prey system with variable harvesting

The human inclination to value rarity contributes to the exploitation of many wildlife species by driving up their market value when their numbers are scarce. This research investigates a model to explore how the value attached to rarity impacts the harvesting of predator species and subsequently influences the dynamics of predator–prey interactions in an ecological system. To broaden the model's scope, it's extended to its nonautonomous form by allowing the harvesting rate of predator species and the degree of neutrality in consumers to respond for the rarity of predator species to change over time. Both the autonomous and nonautonomous systems are explored through analytical and numerical investigation. Numerical findings reveal that as the prey population's growth rate gradually increases, the unforced system shifts from stable boundary equilibrium (harvesting absent) to stable interior equilibrium, then to oscillatory interior equilibrium, followed by period doubling oscillations leading to chaotic behavior. The autonomous system exhibits limit cycle oscillations around the boundary equilibrium when predator species are minimally harvested. Moderate harvesting stabilizes the system, while excessive harvesting induces instability. However, the chaotic oscillations can be eliminated through period‐halving route upon decreasing the consumers' response to predator rarity. Furthermore, the seasonally forced model demonstrates periodic solutions, higher order periodic solutions, and chaotic dynamics contingent upon different settings of prey growth rate, the strength of seasonality in predator harvesting, and the consumers' response to predator rarity.

Optimization of swelling and mechanical behavior of Acer platanoides cellulose combo hydrogel

To reduce resource scarcity and environmental concerns, it is better to choose both sustainable and biodegradable materials for the synthesis of value-added products. In this study, a biodegradable combo hydrogel was developed that is prone to decompose and possesses high swello-mechanical properties. The combo hydrogel is fabricated through crosslinking of Acrylic acid (AA) and n-isopropyl acrylamide monomers on Acer platanoides cellulose (APC) in the presence of MBA (N, N-methylene bisacrylamide). The APC-combo hydrogel was characterized through SEM, FTIR, swelling, mechanical, and biodegradability tests and got results in favor. SEM images show high crosslinking density and porous surface that facilitates water penetration for swelling behavior. The potential use of cellulose in combo hydrogel helped in intensifying its mechanical and biodegradable properties. APC-combo hydrogel (APC-CH9) has a higher swelling ratio at 2:1 monomer to APC ratio, 0.5 percent MBA concentration, 70% neutralization degree, 40 °C temperature, and pH 6. Similarly, the hydrogel possesses higher values for tensile strength and Young's modulus at 0.6 percent MBA concentration but shows higher compression strength and elongation at break at 0.2% MBA. The high swello-mechanical and moderate biodegradability properties of combo hydrogel make it a promising material for minimizing the agricultural water and nutrient leaching issues and results in reducing plant wither rate.

Influence of the Neutralization Degree on the Ice Nucleation Ability of Ammoniated Sulfate Particles

AbstractPrevious laboratory measurements suggest that ammonium sulfate crystals (AS, (NH4)2SO4) are efficient ice‐nucleating particles under cirrus conditions. Sulfate particles not completely neutralized by ammonium are less well studied and include two other solids, ammonium bisulfate (AHS, NH4HSO4) and letovicite (LET, (NH4)3H(SO4)2). In this work, we have obtained the first comprehensive data set for the heterogeneous ice nucleation ability of crystallized particles in the AS–LET–AHS system as a function of their degree of neutralization at a temperature of about 220 K. Quantitative data on nucleation onsets, ice‐active fractions, and ice nucleation active surface site densities were derived from expansion cooling experiments in a large cloud chamber and measurements with two continuous flow diffusion chambers. We found a strong dependence of the efficiency and the mode of heterogenous ice nucleation on the degree of neutralization. Ice formation for AS, mixed AS/LET, and LET crystals occurred by the deposition nucleation or pore condensation and freezing mode. The lowest nucleation onset was observed for AS, where 0.1% of the particles became ice‐active at an ice saturation ratio of 1.25. This threshold gradually increased to 1.35 for LET, and abruptly further to 1.45 for mixed LET/AHS crystals, which partially deliquesced and induced ice formation via immersion freezing. Pure AHS crystals did not form due to the inhibition of efflorescence. Our data allow for a more sophisticated treatment of ice formation in the AS–LET–AHS system in future model simulations, which have so far only considered the available data for AS alone.

TOPSIS method based on q-rung orthopair picture fuzzy soft environment and its application in the context of green supply chain management

<abstract> <p>Green supplier selection has been an important technique for environmental sustainability and reducing the harm of ecosystems. In the current climate, green supply chain management (GSCM) is imperative for maintaining environmental compliance and commercial growth. To handle the change related to environmental concern and how the company manages and operates, they are integrated the GSCM into traditional supplier selection process. The main aims of this study were to outline both traditional and environmental criteria for selecting suppliers, providing a comprehensive framework to assist decision-maker in prioritizing green supplier effectively. In order to address issue to simulate decision-making problems and manage inaccurate data. A useful technique of fuzzy set was proposed to handle uncertainty in various real-life problems, but all types of data could not be handled such as incomplete and indeterminate. However, several extensions of fuzzy set were considered, such as intuitionistic fuzzy set, Pythagorean fuzzy set, q-rung orthopair fuzzy set, and q-rung orthopair fuzzy soft set considering membership and nonmember ship grade to handle the uncertainty problem. However, there was a lack of information about the neutral degree and parameterization axioms lifted by existing approaches, so to fill this gap and overcome the difficulties Ali et al. proposed a generalized structure by combining the structure of picture fuzzy set and q-rung orthopair fuzzy soft set, known as q-rung orthopair picture fuzzy soft sets, characterized by positive, neutral and negative membership degree with parameterization tools and aggregation operator to solve the multi criteria group decision-making problem. Additionally, the TOPSIS method is a widely utilized to assist individuals and organizations in selecting the most appropriate option from a range of choices, taking into account various criteria. Finally, we demonstrate an illustrative example related to GSCM to enhance competitiveness, based on criteria both in general and with a focus on environmental consideration, accompanied by an algorithm and flow chart.</p> </abstract>

Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19.

COVID-19 is a global pandemic caused by the highly infectious SARS-CoV-2 virus. Efforts to combat SARS-CoV-2 infection include mass vaccination and development of monoclonal and convalescent plasma therapeutics that require precise measurements of correlative, functional neutralizing antibodies that prevent virus infection. Developing rapid, safe, easy-to-use, and high-quality neutralization assays are essential for the success of the massive effort. Here, we developed a vesicular stomatitis virus-based neutralization assay that was capable of quantifying varying degrees of neutralization in patient serum samples. This assay has two detection readouts, flow cytometry and live cell imaging. The two readout methods produced consistent values of all 50% neutralization titers, further enhancing measurement confidence on the assay. Moreover, the use of available reference standards such as the World Health Organization International Standard (NIBSC code 20/136) enables quantification and standardization of the pseudovirus neutralization assay with neutralizing antibody titers measured in International Units/mL. Quantitative and standardized neutralization assays are critical for reliable efficacy evaluation and comparison of numerous vaccines and therapeutics.