Soil Organic Matter Degradation Research Articles

Sustainable remediation of acid mine drainage (AMD)- and crude oil-contaminated sites through wastewater-enhanced Pseudomonas aeruginosa ATCC 15442 treatment

The increasing rate of soil contamination poses a major challenge to the sustainability of the ecosystem. Acid mine drainage (AMD) and crude oil are among the major soil contaminants contributing to the degradation of soil organic matter. This study aims to evaluate the application of Pseudomonas aeruginosa ATCC 15442 (PA-15442) and wastewater for the treatment of AMD- and crude oil-contaminated soils. A microcosm containing 1 kg each of AMD- and crude oil-contaminated soils was inoculated with PA-15442, and brewery and domestic wastewater were added for the bioaugmentation study. The result of the 28-day study conducted under mesophilic conditions showed an average TPH and metal removal efficiency of 58.84% and 52.75%, corresponding to 51.07, 47.29, 59.32, 58.98, and 47.1% for the individual metals (Fe, Al, Cu, Zn, and Mn), respectively, and 49% for the average sulfate removal after the treatment period. This study has shown that bioaugmentation of contaminated soils with the strain of PA-15442, and the addition of wastewater could be an environmentally friendly and sustainable approach for the remediation of AMD- and petroleum-contaminated soils.Graphical

Quantitative and Qualitative Relationships between Phospholipid Fatty Acid Analysis Biomarkers and Lignin in Soil from the Tibetan Plateau (China) under Laboratory Incubation Conditions

Lignin, an organic compound with a complex structure, is formed through the polymerization of structural units linked by carbon–carbon bonds and ether bonds. The question of whether lignin is labile or resistant to biological and chemical degradation in soil, particularly in alpine ecosystems, remains unresolved. To address this knowledge gap, we analyzed the relationship between phospholipid fatty acid biomarkers and the abundance of lignin components in grassland soils from North Tibet, China. Soil samples were collected from alpine grasslands, including alpine meadows and alpine steppes. The relative abundance of lignin in these alpine grassland soils before and after a 210-day incubation period was measured. Our results indicate that the relative abundance of lignin in the alpine grassland soils decreased during the incubation period. Significant relationships were found between the phospholipid fatty acid biomarkers of bacteria, fungi, Gram-positive bacteria, and Gram-negative bacteria and the relative abundance of lignin components. This research was conducted under laboratory conditions that are optimal for the development of microorganisms but significantly different from the conditions in Tibet. Furthermore, this study contributes to the understanding of soil organic matter degradation and the dynamics of microbial communities in alpine grassland soils in the context of future global warming.

Integrating cover crops and organic amendments to mitigate the limitations of tillage on soil health and cotton productivity

AbstractVarious tillage systems have limitations on soil health, such as the degradation of soil structure and organic matter under conventional tillage (CT) systems, as well as short‐term soil compaction in conservation tillage systems. A 3‐year field experiment was established to evaluate the integration of cover crop (CC) and organic amendments (OAs) into CT and strip tillage (ST) systems, and their impact on soil properties and cotton (Gossypium hirsutum L.) productivity. The CC was cereal rye (Secale cereale), and the combined application of animal manure and biochar constituted the OA. In the third year, differences in soil compaction between the CT and ST systems were observed when the measurements were made after tillage. Moreover, integrating CC and OA under the CT and ST systems increased the soil depth to compaction zones. Soil compaction was observed at 27.5‐cm depth under CT, at 30‐cm depth under CT integrated with CC and OA, at 10‐cm depth under ST, and at 15‐cm depth under ST integrated with CC and OA, using 2 MPa as the threshold. In general, the integration of CC and OA tended to increase soil respiration, organic matter, and available nutrients, but the effects were not consistent across years and soil depth. Despite differences in the various soil health properties, the management systems had minimum impact on cotton productivity and fiber quality, indicating the ST was effective in preparing the seedbed. Moreover, the differences in soil properties were not at yield‐limiting levels within 3 years of the study.

Karst carbon sink mechanism and its contribution to carbon neutralization under land- use management

The chemical weathering process of carbonate rocks consumes a large quantity of CO2. This has great potential as a carbon sink, and it is one of a significant pathway for achieving carbon neutrality. However, the control mechanisms of karst carbon sink fluxes are unclear, and there is a lack of effective and accurate accounting. We took the Puding Shawan karst water‑carbon cycle test site in China, which has identical initial conditions but different land use types, as the research subject. We used controlled experiments over six years to evaluate the mechanisms for the differences in hydrology, water chemistry, concentrations and fluxes of dissolved organic carbon (DOC) and dissolved inorganic carbon (DIC). We found that the transition from rock to bare soil to grassland led to increases in the DIC concentration by 0.08–0.62 mmol⋅L−1. The inorganic carbon sink flux (CSF) increased by 3.01–5.26 t⋅C⋅km−2⋅a−1, an increase amplitude of 30–70 %. The flux of dissolved organic carbon (FDOC) increase by 0.28 to 0.52 t⋅C⋅km−2⋅a−1, an increase amplitude of 34–90 %. We also assessed the contribution of land use modifications to regional carbon neutrality, it indicate that positive land use modification can significantly regulate the karst carbon sink, with grassland having the greatest carbon sequestration ability. Moreover, in addition to DOC from soil organic matter degradation, DOC production by chemoautotrophic microorganisms utilizing DIC in groundwater may also be a potential source. Thus, coupled studies of the conversion of DIC to DOC processes in groundwater are an important step in assessing karst carbon sink fluxes.

Enhanced response of soil respiration to experimental warming upon thermokarst formation

As global temperatures continue to rise, a key uncertainty of terrestrial carbon (C)–climate feedback is the rate of C loss upon abrupt permafrost thaw. This type of thawing—termed thermokarst—may in turn accelerate or dampen the response of microbial degradation of soil organic matter and carbon dioxide (CO2) release to climate warming. However, such impacts have not yet been explored in experimental studies. Here, by experimentally warming three thermo-erosion gullies in an upland thermokarst site combined with incubating soils from five additional thermokarst-impacted sites on the Tibetan Plateau, we investigate how warming responses of soil CO2 release would change upon upland thermokarst formation. Our results show that warming-induced increase in soil CO2 release is ~5.5 times higher in thermokarst features than the adjacent non-thermokarst landforms. This larger warming response is associated with the lower substrate quality and higher abundance of microbial functional genes for recalcitrant C degradation in thermokarst-affected soils. Taken together, our study provides experimental evidence that warming-associated soil CO2 loss becomes stronger upon abrupt permafrost thaw, which could exacerbate the positive soil C–climate feedback in permafrost-affected regions.

Soil Fertility and Bacterial Community Composition in Response to the Composting of Biochar-Amended Chicken Manure

Amidst the burgeoning expanse of the poultry sector, the escalation of chicken manure production has ensued, potentially exacerbating ecological contamination. However, the application of chicken manure is bound to transmute the habitat of edaphic microorganisms, precipitating an alteration in the soil’s microbial consortium. The composting of biochar-amended chicken manure and wood chips (biochar composting products, BCPs) was used to improve Chinese cabbage (Brassica campestris L.) production and regulate soil properties and bacterial community structure. On the 40th day of Chinese cabbage growth, soil and Chinese cabbage were collected for laboratory analysis. The effects of different proportions of BCPs (0, 1%, 3%, 5% and 7% biochar) on soil fertility, enzyme activity, the microbial community and the growth of Chinese cabbage were studied under facility conditions. The results showed that the growth performance and quality of Chinese cabbage were significantly increased with increasing BCP ratios. Soil fertility indicators including pH, AN, AP, AK and SOM were significantly increased, except for the pH value in the 1% BCP group. The activities of phosphatase, catalase and urease were increased for all groups of BCP treatment. The soil microbial community response was significantly different, and the application of 5% and 7% BCPs reduced the abundance, diversity and evenness of soil bacteria. Chinese cabbage growth performance was positively correlated with an increase in BCP supplemental levels in the range of 3–5%. Also, the abundance, diversity and uniformity of the soil microbial community were improved in the 3% BCP treatment group. Therefore, the dominant bacterial phyla were Bacteroidetes, Actinobacteriota, Gemmatimonadota, Myxococcota, Bdellovibrionota and Firmicutes, especially the Bradyrhizobium of Proteobacteria. BCP treatment reduced the degradation of soil organic matter. In addition, it also improved the relative abundance of sequences associated with improving soil fertility. Collectively, these findings offer insights for the re-evaluation of application management strategies for BCP organic fertilizers.

Conversion of Natural Soil to Paddy Promotes Soil Organic Matter Degradation in Small-Particle Fractions: δ13C and Lipid Biomarker Evidence

The stabilization mechanism of soil organic matter (SOM) has received considerable attention. It is widely accepted that mineral sorption/protection is important for SOM stabilization. However, it remains unclear which organic carbon component is beneficial for mineral protection. We collected soil samples from a paddy field (TP) to compare with natural soil (NS). To illustrate the behavior of different SOM pools and their protection by particles, we separated the soils into different particle-size fractions and then removed the active minerals using an acid mixture (1 M HCl/10% HF). The different carbon pools were analyzed using stable carbon isotopes and lipid biomarkers. Our study showed that acid treatment evidently increased the extractability of free lipids, usually over 60%, which confirmed the predominant role of minerals in SOM protection. For NS, the δ13C values increased with decreasing soil particle sizes and soil depths, indicating that 13C-enriched SOM was selectively preserved. However, this trend disappeared after cultivation, which was mainly attributed to the combined effects of the input of 13C-depleted fresh SOM and decomposition of the preserved 13C-enriched SOM. Meanwhile, based on the degradation parameters of the overall lipid biomarkers, SOM showed higher degradation states in clay and silt fractions than in the sand fraction before cultivation. It is possible that the small particle-size fractions could selectively absorb highly degraded SOM. The clay-associated SOM showed a low degradation state, but its carbon content was low after cultivation. We propose that the previously protected SOM was degraded after cultivation and was replaced by relatively fresh SOM, which should be carefully monitored during SOM management.

Soil organic matter degradation and methylmercury dynamics in Hg-contaminated soils: Relationships and driving factors

Biodegradation of soil organic matter (SOM), which involves greenhouse gas (GHG) emissions, plays an essential role in the global carbon cycle. Over the past few decades, this has become an important research focus, particularly in natural ecosystems. SOM biodegradation significantly affects contaminants in the environment, such as mercury (Hg) methylation, producing highly toxic methylmercury (MeHg). However, the potential link between GHG production from SOM turnover in contaminated soils and biogeochemical processes involving contaminants remains unclear. In this study, we investigated the dynamics of GHG, MeHg production, and the relationship between biogeochemical processes in soils from two typical Hg mining sites. The two contaminated soils have different pathways, explaining the significant variations in GHG and MeHg production. The divergence of the microbial communities in these two biogeochemical processes is essential. In addition to the microbial role, abiotic factors such as Hg species can significantly affect MeHg production. On the other hand, we found an inverse relationship between CH4 and MeHg, suggesting that carbon emission reduction policies and management could inadvertently increase the MeHg levels. This highlights the need for an eclectic approach to organic carbon sequestration and contaminant containment. These findings suggest that it is difficult to establish a general pattern to describe and explain the SOM degradation and MeHg production in contaminated soils within the specific scenarios. However, this study provides a case study and helpful insights for further understanding the links between environmental risks and carbon turnover in Hg mining areas.

Protists regulate microbially mediated organic carbon turnover in soil aggregates

AbstractSoil protists, the major predator of bacteria and fungi, shape the taxonomic and functional structure of soil microbiome via trophic regulation. However, how trophic interactions between protists and their prey influence microbially mediated soil organic carbon turnover remains largely unknown. Here, we investigated the protistan communities and microbial trophic interactions across different aggregates‐size fractions in agricultural soil with long‐term fertilization regimes. Our results showed that aggregate sizes significantly influenced the protistan community and microbial hierarchical interactions. Bacterivores were the predominant protistan functional group and were more abundant in macroaggregates and silt + clay than in microaggregates, while omnivores showed an opposite distribution pattern. Furthermore, partial least square path modeling revealed positive impacts of omnivores on the C‐decomposition genes and soil organic matter (SOM) contents, while bacterivores displayed negative impacts. Microbial trophic interactions were intensive in macroaggregates and silt + clay but were restricted in microaggregates, as indicated by the intensity of protistan‐bacterial associations and network complexity and connectivity. Cercozoan taxa were consistently identified as the keystone species in SOM degradation‐related ecological clusters in macroaggregates and silt + clay, indicating the critical roles of protists in SOM degradation by regulating bacterial and fungal taxa. Chemical fertilization had a positive effect on soil C sequestration through suppressing SOM degradation‐related ecological clusters in macroaggregate and silt + clay. Conversely, the associations between the trophic interactions and SOM contents were decoupled in microaggregates, suggesting limited microbial contributions to SOM turnovers. Our study demonstrates the importance of protists‐driven trophic interactions on soil C cycling in agricultural ecosystems.

Long-Term Nitrogen Addition Could Modify Degradation of Soil Organic Matter through Changes in Soil Enzymatic Activity in a Natural Secondary Forest

Soil extracellular enzymes play a key role in mediating the degradation of soil organic matter, but little is understood as to how the pattern of soil extracellular enzymes could be altered by nitrogen (N) addition. In this study, the effects of N addition (started from 2006, four treatments: control, 0 g N·m–2·yr–1; low N addition, 2.5 g N·m–2·yr–1; high N addition, 5.0 g N·m–2·yr–1) on soil extracellular enzymes and microbial biomass in a natural secondary forest of Northeastern China. The results showed that the activity of urease, sucrase, peroxidase and N-acetyl-β-D-glucosidase decreased with N addition, and the activity of acid phosphatase and leucine aminopeptidase increased significantly with N addition. Soil total N, temperature at 5 cm soil depth, pH value, microbial biomass carbon and microbial biomass N were the key factors affecting soil enzyme activity. In summary, the enzyme activity related to soil organic matter degradation shows a decreasing trend under N addition. The results suggest that the increase in N deposition will slow down the degradation of soil organic matter in natural secondary forest, which is more conducive to the accumulation of organic matter in the soil.

Soil carbon plays a role in the climate impact of diet and its mitigation: the Finnish case

IntroductionDiet has a significant impact on the consumer’s climate impact, and a radical global change in the food system is necessary. However, the change needs to be interpreted and adapted to local conditions.MethodsTo support national climate policy, we evaluated current Finnish diet and its four alternatives: “current diet”; “meat to half diet”; “meat to a third diet”; “a diet rich in fish and milk”; and “a vegan diet”. We created the FoodMin model to simultaneously address both climate impacts and nutrient uptake and to combine carbon dioxide (CO2) emissions from soil organic matter (SOM) degradation with dietary climate impact assessments. As a well-established assessment method remains lacking, product group-specific estimates for the CO2 emissions of SOM origin were produced in two different ways, based on long-term observational data or by modelling with the Yasso07. We also examined, using three scenarios, how much the achievement of soil carbon (C) balance in Finnish production could affect the climate impact of the diet.Results and DiscussionThe climate impact of the current diet was 6.0 kg CO2 per person per day, and for alternative diets, the change compared with the current diet was −14%, −20%, −31%, and −39% respectively, for “meat to half” (5.2 kg CO2 eq. per person per day), “meat to third” (4.8), “a diet rich in fish and milk” (4.2), and “the vegan diet” (3.7). SOM-derived CO2 emissions from domestic fields accounted for 18% of the climate impact of the current diet and up to 23% of alternative diets. In terms of the soil C-balance scenarios, all actions together could mean a 3–13% reduction in the climate impact of the diet: the more products of animal origin, the more reduction opportunities in the diet. With the change in diet, these measures could reduce the climate impact of diets by 13–41%. The nutritional value of a “diet rich in fish and milk” was the best. The study revealed that SOM-induced CO2 emissions and SOM-related activities play a very important role in the climate impact of the diet and its mitigation; they cannot be ignored in dietary assessments in addition to direct product choices.

Effect of Inorganic Fertilizers and Organic Manures on Physical Properties of Soil: A Review

Deterioration of physical properties of soil and there after depletion the soil fertility are the main constrains in food and environment security of any country. This review paper summarizes the current knowledge and information on the effect of inorganic fertilizers and organic manures on soil physical properties. The use of inorganic fertilizer or organic fertilizer alone has both positive and negative effects on soil properties, plant growth and nutrient availability. Most of earlier investigators confirmed that combined application of inorganic fertilizers and organic manures improved the physical properties and available nutrient status in soils. Organic manures improve physical properties of soil but they are comparatively low in nutrient content, addition of larger quantity of manures is required for successful plant growth. However, inorganic fertilizers is contain all the essential nutrients which are early accessible for plants. Due to continuous use of inorganic fertilizers alone causes soil organic matter degradation, soil acidity or alkalization, soil deterioration and environmental pollution, so integrated or mixed inorganic and organic nutrient management system is an alternative system for the sustainable and cost effective management of soil and the result is improve in soil properties and raising soil fertility without affecting environment. The objective of the review is to assess the effect of inorganic fertilizer and organic manure on physical properties of soil. The study revealed that appropriate application of inorganic fertilizers with organic manures improves soil physical properties and increases the productivity without negative effect on soil health than the values obtained by organic or inorganic fertilizers separately.

Impact of a warmer cold season on soil nitrogen and carbon cycling in High Arctic wet sedge tundra

Soil available nitrogen (N) pools in the High Arctic are expected to increase due to permafrost thaw and elevated microbial activity stimulated by atmospheric warming and subsequent changes in soil moisture regimes. The cold season, or the period between soil freeze-up in fall and thaw in spring, is warming relatively faster compared to the growing season, which may increase the insulating snow cover and microbial degradation of soil organic matter. Changes in temperature and available N levels can stimulate soil CO₂, CH4, and N₂O flux rates which could trigger further warming. Despite the potential importance of the cold season, the influence of variability in cold season temperatures on the release and accumulation of inorganic N and greenhouse gases is poorly understood in High Arctic environments. We conducted a 50-day laboratory incubation study that mimics the annual soil temperature cycle to investigate the effects of different minimum cold season temperatures (- 10˚C, −15˚C, −20˚C, −25˚C) on spring and growing season N and carbon (C) cycling in the soil.We measured inorganic N pools, net N cycling rates, and CO₂, CH4, and N₂O fluxes throughout the study. Over the coldest period of the annum, the warmest and coldest soils displayed greatest net N mineralization rates (-0.035 mg kg−1 d-1 and −0.049 mg kg−1 d-1 respectively). During the thaw period, we found that soils treated with −10˚C resulted in a spring NO3–-N flush that was ∼ 700% larger than −15 ˚C, −20 ˚C, −25 ˚C treatments, which were relatively consistent with each other, suggesting a cold-temperature inhibition of nitrification. Although cold season fluxes of CH4 and CO₂ differed with temperature, there was no treatment effect on subsequent flux rates during the growing season. We conclude that changes in cold season temperatures can play a crucial role in soil N biogeochemistry in High Arctic wet-sedge communities and have a potential to disrupt surrounding downstream ecosystems. The impact of cold season warming, however, does not appear to be linear for all processes with temperature change.

Enhancing Soil Organic Matter Transformation through Sustainable Farming Practices: Evaluating Labile Soil Organic Matter Fraction Dynamics and Identifying Potential Early Indicators

The growing global population and increasing demand for agricultural products have exerted significant pressure on agricultural systems. As a result, soil organic matter depletion and degradation have become prevalent issues, including in regions such as South Bohemia, Czech Republic, where conventional farming practices are predominant. Soil organic matter (SOM) plays a critical role in soil health, crop productivity, and the sustainability of agricultural systems, with changes occurring in both the total and labile fractions of the organic matter pools. However, changes in the total soil organic matter carbon pool (TOC) resulting from agricultural practices occur gradually and may become evident only after several years, posing challenges for timely management adjustments. Therefore, the identification of early indicators of SOM dynamics is crucial for implementing prompt corrective actions. The aim of this study was to evaluate the effects of sustainable management practices, such as cultivated crops (Pisum sativum and Lupinus albus), selected entomopathogenic and myco parasitic fungi (MEHA) (Trichoderma virens and Metarhizium brunneum), and lactic acid bacteria (LAB) on the labile fraction of the SOM pool (CLSOM) and identify potential early indicators. Our findings demonstrated that the type of crop and applied microorganisms (treatments) significantly affected the CLSOM in peas, and the crop growth stages affected the TOC in both pea and lupin. Growth stages also showed an impact on the CLSOM in lupin. Moreover, in both crops, the change in CLSOM correlated with changes in the SOM oxidation rate constant (k), carbon lability index (LI), carbon management index (CMI), and carbon enrichment ratio (ER). Conversely, changes in the TOC did not exhibit significant correlations, except for LI and CMI, which showed a positive correlation with the TOC in peas (p < 0.05). Furthermore, the separate application of MEHA and LAB on seeds or leaves resulted in increased SOM carbon pools compared with the combined application. The application of these beneficial microorganisms in pea and lupin crops showed potential in maintaining or increasing CLSOM, which can be assessed early through indicators such as k, LI, CMI, and ER. Contributing to the development of sustainable soil management strategies, future research should further investigate different crops and microorganisms—and the mechanisms underlying their observed relationships—and explore additional early indicators to refine and optimize sustainable agricultural practices.

Effect of reduced inherent organic matter on stability and transport behaviors of black soil colloids

Soil organic matter plays an important role in the stability, transport, and fate of soil colloids. At present, studies have mostly focused on the effects of adding exogenous organic matter on soil colloidal properties, while there is very limited research on the effect of reduced inherent soil organic matter on the environmental behavior of soil colloids. This study investigated the stability and transport behavior of black soil colloids (BSC) and black soil colloids with reduced inherent organic matter (BSC-ROM) under different ionic strength (5, 50 mM) and background solution pH (4.0, 7.0, and 9.0) conditions. Meanwhile, the release behavior of two soil colloids in the saturated sand column under transient ionic strength conditions was also studied. The results showed that both ionic strength reduction and pH increase increased the negative charges of BSC and BSC-ROM, and improved the electrostatic repulsion between soil colloids and grain surface, thereby promoting the stability and mobility of soil colloids. The decrease in inherent organic matter had little effect on the surface charge of soil colloids, suggesting that the electrostatic repulsion was not the main force affecting the stability and mobility of BSC and BSC-ROM, and reducing inherent organic matter might significantly reduce the stability and mobility of soil colloids by weakening the steric hindrance interaction. The decrease of transient ionic strength reduced the depth of the energy minimum and activated the soil colloids retained on the surface of the grain at three pH conditions. This study is helpful to predict the potential impact of soil organic matter degradation on the fate of BSC in natural environment system.

Coprecipitation with Ferrihydrite Inhibits Mineralization of Glucuronic Acid in an Anoxic Soil.

It is known that the association of soil organic matter (SOM) with iron minerals limits carbon mobilization and degradation in aerobic soils and sediments. However, the efficacy of iron mineral protection mechanisms under reducing soil conditions, where Fe(III)-bearing minerals may be used as terminal electron acceptors, is poorly understood. Here, we quantified the extent to which iron mineral protection inhibits mineralization of organic carbon in reduced soils by adding dissolved 13C-glucuronic acid, a 57Fe-ferrihydrite-13C-glucuronic acid coprecipitate, or pure 57Fe-ferrihydrite to anoxic soil slurries. In tracking the re-partitioning and transformation of 13C-glucuronic acid and native SOM, we find that coprecipitation suppresses mineralization of 13C-glucuronic acid by 56% after 2 weeks (at 25 °C) and decreases to 27% after 6 weeks, owing to ongoing reductive dissolution of the coprecipitated 57Fe-ferrihydrite. Addition of both dissolved and coprecipitated 13C-glucuronic acid resulted in increased native SOM mineralization, but the reduced bioavailability of the coprecipitated versus dissolved 13C-glucuronic acid decreased the priming effect by 35%. In contrast, the addition of pure 57Fe-ferrihydrite resulted in negligible changes in native SOM mineralization. Our results show that iron mineral protection mechanisms are relevant for understanding the mobilization and degradation of SOM under reducing soil conditions.

The patchiness of soil 13C versus the uniformity of 15N distribution with geomorphic position provides evidence of erosion and accelerated organic matter turnover

Farming on hillslopes often affects the accumulation and loss of soil organic matter (SOM) depending on slope position and cropping patterns. Most hillslope studies focus on soil movement to characterize SOM turnover under erosive conditions. In this study, we trace erosion and characterize agronomic practices erosive impacts on SOM translocation and transformation along geomorphic positions. To achieve this, we assessed the horizontal distribution (upper 15 cm) and vertical distribution (to 100 cm profiles) of soil δ15N and δ13C isotope abundance individually. We mapped the spatial distribution of δ13C, δ15N, and SOM turnover indices as a novel approach to tracing erosion and degradation of SOM in the field. Except for tillage (conventional vs. reduced tillage), other individual agricultural practices (residue removal with no cover crop vs. retaining residuals, cover cropping, and fertilizer 0, 40, and 80 kg ha-1 nitrogen) caused no significant shifts in δ15N and δ13C values in topsoil (0–15 cm). Among the evaluated factors, topography and depth predicted soil δ15N and δ13C profiles. Trends in δ13C vs. δ15N showed a wider range of δ13C values in topsoil of upslope plots under reduced tillage, while in the depositional location, conventional tillage had the same effect. This suggests erosion under reduced tillage occurred. Erosion and accelerated decomposition gradually slowed δ13C enrichment with soil depth. Digital soil mapping approach depicted low continuity of δ13C vs. high continuity of δ15N with geomorphic position We attributed the intermediate δ13C values, and steeper slope of δ13C against logarithm of soil organic carbon (SOC) across the slope to erosion and high SOM turnover, particularly of recently added plant inputs. Current results support the prediction of intensive vs. conservation practices’ effects on upslope soil stability and the fate of SOM in both topsoil and at depth of sloping farmlands.

Porewater constituents inhibit microbially mediated greenhouse gas production (GHG) and regulate the response of soil organic matter decomposition to warming in anoxic peat from a Sphagnum-dominated bog.

Northern peatlands store approximately one-third of terrestrial soil carbon. Climate warming is expected to stimulate the microbially mediated degradation of peat soil organic matter (SOM), leading to increasing greenhouse gas (GHG; carbon dioxide, CO2; methane, CH4) production and emission. Porewater dissolved organic matter (DOM) plays a key role in SOM decomposition; however, the mechanisms controlling SOM decomposition and its response to warming remain unclear. The temperature dependence of GHG production and microbial community dynamics were investigated in anoxic peat from a Sphagnum-dominated peatland. In this study, peat decomposition, which was quantified by GHG production and carbon substrate utilization is limited by terminal electron acceptors (TEA) and DOM, and these controls of microbially mediated SOM degradation are temperature-dependent. Elevated temperature led to a slight decrease in microbial diversity, and stimulated the growth of specific methanotrophic and syntrophic taxa. These results confirm that DOM is a major driver of decomposition in peatland soils contains inhibitory compounds, but the inhibitory effect is alleviated by warming.

A Data-Validated Stoichiometric Model for the Priming Effect.

Due to global warming, interest in sequestering carbon by appropriately managing soils has contributed to studying the dynamic exchange of carbon and nitrogen in soils and atmospheric CO[Formula: see text]. The priming effect, or the intensified CO[Formula: see text] emissions from soil organic matter (SOM) decomposition in short periods by using labile substrates, has been a topic of interest over the last decades. A combination of two experimentally supported mechanisms explains the priming effect phenomenon, and for the first time, we combine them in a novel stoichiometric model. The model considers the effects of labile substrate utilization in soils during the SOM decomposition and how CO[Formula: see text] emissions rates are affected. Laboratory data and a local sensitivity analysis validate the accuracy and robustness of the model. We find an optimized ratio of labile carbon and nitrogen that intensifies SOM decomposition for different soil features. The priming effect is weakened as C/N in SOM increases for nutrient-poor soils and is independent of C/N in SOM for nutrient-rich soils. The time required for microorganisms to decompose SOM at its maximum rate is delayed only for labile carbon treatments and poor-nutrient soils but remains constant otherwise. Finally, the SOM degradation efficiency determines the priming effect's acceleration or reduction under different soil treatments.

Soil enzyme kinetics and thermodynamics in response to long-term vegetation succession

Our current knowledge regarding soil organic matter (SOM) turnover during vegetation succession is often limited to conventional C decomposition models. However, microbial enzyme-mediated SOM degradation and nutrient cycling are mainly reflected in the kinetic parameters of these enzymes. Changes in the composition and structure of plant communities are typically accompanied by alterations in soil ecological functions. Therefore, it is important to clarify the kinetic parameters of soil enzymes and their temperature sensitivity in response to vegetation succession, especially under the current trend of climate change-related global warming; however, these are still understudied. Here, we examined the kinetic parameters of soil enzymes, their temperature sensitivity, and their associations with environmental variables over long-term (approximately 160 years) vegetation succession on the Loess Plateau using a space-for-time substitution method. We found that the kinetic parameters of soil enzymes changed significantly during vegetation succession. Specific response characteristics varied depending on the enzyme. Overall, the temperature sensitivity (Q10, 0.79–1.87) and activation energy (Ea, 8.69–41.49 kJ·mol−1) remained stable during long-term succession. Compared with N-acetyl-glucosaminidase and alkaline phosphatase, β-glucosidase was more sensitive to extreme temperatures. In particular, two kinetic parameters (i.e., maximum reaction rate, Vmax; half-saturation constant, Km) of β-glucosidase were decoupled at low (5 °C) and high (35 °C) temperatures. Overall, Vmax was the primary determinant of variations of enzyme catalytic efficiency (Kcat) during succession, and soil total nutrients had a greater impact on Kcat than available nutrients. Our results suggested that soil ecosystems played an increasingly important role as a C source during long-term vegetation succession, as indicated by the positive responses of the C cycling enzyme Kcat, while the factors related to soil N and P cycling remained relatively stable.