Degradation Of Nanoparticles Research Articles

Unveiling the power of liquid chromatography in examining a library of degradable poly(2-oxazoline)s in nanomedicine applications.

A library of degradable poly(2-alkyl-2-oxazoline) analogues (dPOx) with different length of the alkyl substituents was characterized in detail by gradient elution liquid chromatography. The hydrophobicity increased with increased side chain length as confirmed by a hydrophobicity row, established by reversed-phase liquid chromatography. Those dPOx were cytocompatible and formed colloidally stable nanoparticle (NP) formulations with positive zeta potential. Dynamic light scattering (DLS) revealed that dPOx with increased hydrophobicity tended to form NPs with increased sizes. NPs created from the most hydrophobic polymer, degradable poly(2-nonyl-2-oxazoline) (dPNonOx), showed tendency for aggregation at pH 5.0, and in the presence of protease in solution, in particular for NPs formulated without surfactant. Liquid chromatography revealed enzymatic degradation of dPNonOx NPs, clearly demonstrating the disappearance of polymer signals and the appearance of hydrophilic degradation products eluting close to the chromatographic void time. The degradation process was confirmed by 1H NMR spectroscopy. dPNonOx NPs containing the anti-inflammatory drug BRP-201 as payload reduced 5-lipoxygenase activity in human neutrophils. Thereby, composition analysis of the resultant NPs, including drug quantification, was also enabled by liquid chromatography. The results indicate the importance of a detailed analysis of the final polymer-based NP formulations by a multimethod approach, including, next to standard applied techniques such as DLS/ELS, the underexplored potential of liquid chromatography. The latter is demonstrated to resolve a fine structure of solution composition, together with an assessment of possible degradation pathways and is versatile in determining hydrophobicity/hydrophilicity of polymer materials. Our study underscores the power of liquid chromatography for characterization of soft matter drug carriers.

Inhibitory effects of chlorhexidine-loaded calcium carbonate nanoparticles against dental implant infections

This study aimed to design sustained released biodegradable calcium carbonate nanoparticles loaded with chlorhexidine (CHX-loaded NPs) and to investigate the early osteogenic differentiation and antimicrobial effects on the important bacteria involved in infections of dental implants. The microemulsion method was used to prepare the calcium carbonate nanoparticles loaded with chlorhexidine. The prepared nanoparticles were characterized using conventional methods. The release pattern determination and the biodegradation test were performed for the prepared nanoparticles. For the early osteogenic differentiation test of the prepared nanoparticles, alkaline phosphatase (ALP) activity was detected in human dental pulp stem cells (HDPSCs). The antimicrobial effects of the nanoparticles were evaluated against Escherichia coli, Streptococcus mutans, Enterococcus faecalis, Staphylococcus aureus, and Pseudomonas aeruginosa. The sizes of free calcium carbonate nanoparticles and CHX-loaded NPs were 105 ± 1.63 and 118 ± 1.47 nm and their zeta potentials were − 27 and − 36, respectively. A 50% degradation of nanoparticles was achieved after 100 days. These nanoparticles showed a two-stage sustained release pattern in vitro. Microscopic images revealed that the morphology of free calcium carbonate nanoparticles primarily took on a spherical calcite form, while CHX-loaded NPs predominantly exhibited a cauliflower-like vaterite polymorph. The nanoparticles increased the activity of ALP in cells in two weeks significantly (p < 0.05). Antimicrobial and antibiofilm results showed an efficient effect of the prepared nanoparticle against the studied bacteria. Calcium carbonate nanoparticles are an efficient multifunctional vector for chlorhexidine and can be used as a bioactive antibacterial agent against various oral microorganisms to prevent implant infections.

A multi-analytical approach to evaluate the removal efficiency of polystyrene nanoparticles in water treatment processes

The removal of nanoplastics (NP) from water using various treatment processes has gained significant attention recently. This study comprehensively characterizes the degradation of polystyrene nanoparticles (concentration: 200 ppm, diameter: 140 nm) through UVC irradiation. For the first time, we compared four analytical methods to monitor removal efficiency: Py-GCMS, UV–Visible spectroscopy, TOC, and Turbidity. Additionally, DLS, TEM, and SEC were used to understand changes in particle size, morphology, and molecular weight. Results showed that Py-GCMS overestimated the removal rate by a factor of 2 compared to Turbidity and UV–Visible measurements, which were in agreement. Furthermore, after 200 h of irradiation, the styrene signal disappears from the pyrogram, although the mineralization rate reaches only 50%, as determined by total organic carbon (TOC) analysis. The particle size decreased slowly, reaching 100 nm after 150 h, while a significant decrease in molecular weight indicated high chain-scission. These findings emphasize the importance of a multi-analytical approach to accurately assess NP removal efficiency and understand degradation mechanisms.

A Three–Dimensional Nanoscale View of Electrocatalyst Degradation in Hydrogen Fuel Cells

AbstractThe loss of platinum (Pt) electrochemically active surface area (ECSA) is a critical degradation mode that often becomes a limiting factor for heavy‐duty proton exchange membrane fuel cell vehicles. High surface area carbon supports have been shown to improve Pt dispersion and limit detrimental ionomer‐electrocatalyst interactions due to their large interior pore volume. In this work, using automated scanning transmission electron tomography, the degradation of nanoparticles located on the interior versus exterior surfaces of the carbon support is compared following a catalyst‐specific accelerated stress test (AST) of 90,000 voltage cycles between 0.6 V to 0.95 V. The results reveal a notable increase in median particle size for both interior and exterior Pt catalyst particles, with a slightly higher increase in particle size distribution and loss of specific surface area for the particles located on the exterior carbon surface. The fraction of Pt nanoparticles that reside within the interior of the carbon support also increased following the AST test, accompanied by evidence of an increase in average carbon mesopore size. The results shed light on the degradation mechanisms affecting electrochemical properties and the enhanced particle accessibility at lower relative humidity.

Physicochemical Changes of Apoferritin Protein during Biodegradation of Magnetic Metal Oxide Nanoparticles.

The biodegradation of therapeutic magnetic-oxide nanoparticles (MONPs) in the human body raises concerns about their lifespan, functionality, and health risks. Interactions between apoferritin proteins and MONPs in the spleen, liver, and inflammatory macrophages significantly accelerate nanoparticle degradation, releasing metal ions taken up by apoferritin. This can alter the protein's biological structure and properties, potentially causing health hazards. This study examines changes in apoferritin's shape, electrical surface potential (ESP), and protein-core composition after incubation with cobalt-ferrite (CoFe2O4) oxide nanoparticles. Using atomic force microscopy (AFM) and scanning Kelvin probe force microscopy (SKPFM), we observed changes in the topography and ESP distribution in apoferritin nanofilms over time. After 48 h, the characteristic apoferritin hole (∼1.35 nm) vanished, and the protein's height increased from ∼3.5 to ∼7.5 nm due to hole filling. This resulted in a significant ESP increase on the filled-apoferritin surface, attributed to the formation of a heterogeneous chemical composition and crystal structure (γ-Fe2O3, Fe3O4, CoO, CoOOH, FeOOH, and Co3O4). These changes enhance electrostatic interactions and surface charge between the protein and the AFM tip. This approach aids in predicting and improving the MONP lifespan while reducing their toxicity and preventing apoferritin deformation and dysfunction.

Cellulolytic characterization of the rumen-isolated Acinetobacter pittii ROBY and design of a potential controlled-release drug delivery system

A novel cellulolytic bacterial strain, ROBY, was isolated from a bovine rumen sample using the enrichment culture method. This isolate was found to be Acinetobacter pittii, with >99 % similarity according to 16S rRNA gene sequence analysis. The potential use of this strain in combination with doxorubicin (Dox)-integrated cellulose nanoparticles (Dox-CNPs) was evaluated as a proof-of-concept study for the further development of this approach as a novel controlled-release drug delivery strategy. The isolate can utilize CNPs as the sole carbon source for growth and degrade both Dox-CNPs and empty CNPs with high efficiency. Extracellular cellulases isolated from bacteria may also be used to trigger Dox release. The results also demonstrated that the release of Dox into the environment due to nanoparticle degradation in the samples incubated with Dox-CNPs significantly affected bacterial cell viability (∼75 % decrease), proving the release of Dox due to bacterial cellulase activity and suggesting the great potential of this approach for further development.

Photocatalytic activity of Ba, Ca-doped flowerlike ferrates prepared via sol-gel method

Bismuth ferrite (BIFEO), Barium doped bismuth ferrite (BBIFEO) and Calcium doped bismuth ferrite (CBIFEO) nanoparticles (NPs) named BIFEO, BBIFEO and CBIFEO are synthesized via the Sol-gel method. The Scanning electron microscopy (SEM) images showed flower-like morphology. The Ba2+ and Ca2+ dopants have decreased the band gap energy of undoped rhombohedral ferrate NPs leading to a high efficiency for the breakdown of methylene blue under the influence of visible-light. The BIFEO, BBIFEO and CBIFEO NPs showed degraded efficiency of 58 %, 83.4 % and 98.7 % in Methyl blue (MB) dye under Visible-light. The higher degradation of Ca and Ba-doped NPs proved that Ba2+ andCa2+ions have enhanced the photocatalytic activity of undoped spinal ferrite NPs. The doped material is therefore very advantageous for the degradation of dyes under visible-light irradiation, among other photocatalytic applications.

Smart and responsive nano copper oxide–PDMS composite for “three-in-one” synergistic adsorptive–oxidative / reductive degradation of the persistence of organic pollutants, elimination of nanoparticles and heavy metal ion

Copper-and silica-based materials have been used globally as environmental catalysts and adsorbents for centuries. Despite their differences in their properties and roles in the participation of environmental activity, both have been combined to create various types of functional materials, ranging from copper oxides and polydimethylsiloxane (PDMS) sponge (PS) cores to advanced hybrid composite materials. In this work, CuO was incorporated onto SiO2 (CPC) by calcination of PDMS-Cu2O sponge, and the physicochemical properties and application of CPC were investigated. The CPC was homogeneous in a surface area (326.69 m2g-1), pore size (33.16 Å), pore volume (0.056 cm³g−1), and particle size (183.66 Å). This CPC has been used for the degradation (oxidation/reduction) and adsorption of azithromycin (AZM), 1,4-dichlorobenzene (DCB), rhodamine B (RB), methylene blue (MB), 4-nitrophenol (4-NP), gold nanoparticles (AuNPs), and chromium (VI). Efficiencies of CPC for the above persistence of organic pollutants (POPs) and adsorption of AuNPs and Cr(VI) were ∼72–100% under optimized conditions. CPC followed the pseudo-second-order (PSO) degradation kinetics with a higher r2 (>0.99) with eight cycles of reusability. Moreover, this synthetic strategy can be tailored to produce nanostructured composite materials and/or those associated with other species for diverse environmental and industrial applications.

Enhanced photocatalytic degradation and synergistic effects of Tin-Doped zinc oxide nanoparticles for environmental remediation

Using zinc acetate and sodium hydroxide as starting reagents, zinc oxide nanoparticles (NPs) were constructed using a co-precipitation process. Various metal elements were incorporated into the nanocomposites. The synthesized samples underwent calcination at different temperatures for a duration of 2 h. Characterization of the samples was conducted employing XRD, SEM, EDS, and PIXE analysis. SEM imagery revealed diverse morphological alterations of ZnO after doping. The whole width at half the maximum of the XRD peaks, which indicates sizes in the nano range, was used to calculate the average crystallite sizes of the samples using Debye-Scherer’s formula. EDS analysis confirmed the production of highly pure ZnO nanostructures via this method. The synthesized bimetallic and tri-metallic NPs were utilized for the degradation of hazardous organic dyes, with the degradation process monitored using UV–visible spectroscopy. This study underscores the significance of doped ZnO NPs as effective agents for biodegradation.

Degradation and Recondensation of Metal Oxide Nanoparticles in Laminar Premixed Flames.

The behavior of technical nanoparticles at high temperatures was measured systematically to detect morphology changes under conditions relevant to the thermal treatment of end-of-life products containing engineered nanomaterials. The focus of this paper is on laboratory experiments, where we used a Bunsen-type burner to add titania and ceria particles to a laminar premixed flame. To evaluate the influence of temperature on particle size distributions, we used SMPS, ELPI and TEM analyses. To measure the temperature profile of the flame, we used coherent anti-Stokes Raman spectroscopy (CARS). The comprehensible data records show high temperatures by measurement and equilibrium calculation for different stoichiometries and argon admixtures. With this, we show that all technical metal oxide nanoparticle agglomerates investigated reform in flames at high temperatures. The originally large agglomerates of titania and ceria build very small nanoparticles (<10 nm/"peak 2") at starting temperatures of <2200 K and <1475 K, respectively (ceria: Tmelt = 2773 K, Tboil = 3873 K/titania: Tmelt = 2116 K, Tboil = 3245 K). Since the maximum flame temperatures are below the evaporation temperature of titania and ceria, enhanced vaporization of titania and ceria in the chemically reacting flame is assumed.

Empirical correlation to analyze performance of shell and tube heat exchanger using TiO2 Nanofluid-DI water in solar water heater

Recent studies have examined the thermal stability and reliability of TiO2 nanofluid-based heat exchangers under long-term operation in solar water heating systems. This includes assessing nanoparticle sedimentation, agglomeration, and degradation over time, as well as their impact on heat transfer performance and system reliability. In this study heat transfer and fluid flow characteristics of TiO2-DI-H2O (deionized water) nanofluid used in a shell and tube exchanger with different baffle angles under turbulent regimes were numerically explored. The proposed setup was run with nanoparticle concentration of 0 %–0.2 % and Reynolds numbers (Re) from 5000 to 15,000. Baffle angles of 10°, 20°, and 30° were chosen for turbulence analysis. It was observed that nanoparticle concentration strongly impacted the thermophysical properties. The results indicated that adding modest concentrations of TiO2 to DI-H2O significantly improves heat transfer and the Nu. At the Re of 5000 and 30° baffles, the Nu was 58; however, at the Re of 15,000, the Nu value was 88. The improvement in heat transfer of 62.85 %, 70.64 %, and 75.06 % was recorded for baffle angles of 10°, 20°, and 30° respectively, Furthermore, a notable increase in friction factor of 13.6 % at Re 15,000 was observed. However, the ideal baffle angle for maximum thermal performance is 30°. Finally, with shell temperature, it was established that 30° baffle orientation could provide the best h and Nu values and improve the overall performance of the heat exchanger. The obtained Nu was also compared with the existing correlations of literature in the turbulent regime with the aid of nanoparticles and presented for case applications.

Photocatalytic dye degradation, H2 evolution, electrical and mechanical performances of mechano-synthesized multifunctional Bi2Te3 nanoparticles: Effect of milling time and thermal treatment

Bi2Te3 nanoparticles are synthesized by mechanical alloying the Bi and Te powders under the Ar and pressed powdered samples are sintered at 573 K. Rietveld refined XRD patterns, FESEM images and EDX spectra reveal the microstructure, morphology and phase purity of the samples. Bandgaps are obtained from the FTIR spectra and a blue shift with size reduction indicates the size confinement effect. The non Debye type ac conductivity and dielectric constant variations with milling time and sintering are measured. The improved photocatalytic degradation efficiency of the sintered sample (83.59 %) using Rhodamine B dye under visible light elucidates reduced porosity effect. The photocatalytic hydrogen evolution efficiencies of these nanoparticles are measured and a probable mechanism is depicted. The highest H2 generation efficiency (1462.5 μmol g−1h−1) is observed with the sintered sample. Microhardness measurement reveals normal indentation size effect. Empirical correlations among electrical conductivity, microhardness and photodegradation are established for the first time.

Tracking the Cellular Degradation of Silver Nanoparticles: Development of a Generic Kinetic Model.

Understanding the degradation of nanoparticles (NPs) after crossing the cell plasma membrane is crucial in drug delivery designs and cytotoxicity assessment. However, the key factors controlling the degradable kinetics remain unclear due to the absence of a quantification model. In this study, subcellular imaging of silver nanoparticles (AgNPs) was used to determine the intracellular transfer of AgNPs, and single particle ICP-MS was utilized to track the degradation process. A cellular kinetic model was subsequently developed to describe the uptake, transfer, and degradation behaviors of AgNPs. Our model demonstrated that the intracellular degradation efficiency of AgNPs was much higher than that determined by mimicking testing, and the degradation of NPs was highly influenced by cellular factors. Specifically, deficiencies in Ca or Zn primarily decreased the kinetic dissolution of NPs, while a Ca deficiency also resulted in the retardation of NP transfer. The biological significance of these kinetic parameters was strongly revealed. Our model indicated that the majority of internalized AgNPs dissolved, with the resulting ions being rapidly depurated. The release of Ag ions was largely dependent on the microvesicle-mediated route. By changing the coating and size of AgNPs, the model results suggested that size influenced the transfer of NPs into the degradation process, whereas coating affected the degradation kinetics. Overall, our developed model provides a valuable tool for understanding and predicting the impacts of the physicochemical properties of NPs and the ambient environment on nanotoxicity and therapeutic efficacy.

Monitoring the Degradation of Metal‐Organic Framework Drug Nanocarriers by In‐Situ NMR Spectroscopy

AbstractAn accurate characterization methodology is indispensable to design nanoparticle‐based drug delivery system (DDS) adapted to specific diseases and therapies. However, characterization techniques employed to investigate drug release and nanoparticle degradation require separating the nanoparticles from their suspension media, which can lead to artefacts. Therefore, there is a clear need to implement novel versatile in‐situ methods. Here, we report the use of in‐situ NMR spectroscopy to monitor drug delivery processes from MOF nanocarriers, both in solution and in the solid state simultaneously. In‐situ 1H NMR investigation of nanoMIL‐100(Al) suspension in phosphate medium enabled recording the trimesate ligand loss in the solid phase and the ligand release in the liquid phase as a function of time. Simultaneously, 27Al NMR enabled assessing the progressive replacement of carboxylate ligands by phosphates leading to the formation of new aluminum species. Using the same strategy, we also compared the degradation of nanoMIL‐100(Al) loaded with two drug analogs, highlighting an effect of metal‐ligand complexing strength. Furthermore, our in‐situ technique is applicable to studying the reaction of paramagnetic nanoMIL‐100(Fe) in the liquid phase. This work offers an alternative to ex‐situ techniques for understanding the degradation mechanism of MOF nanocarriers and could be an asset for other nanoscale DDSs.

An effective approach to improve photocatalytic dye degradation and electrochemical properties of MoO3 nanoparticles

An effective approach to improve photocatalytic dye degradation and electrochemical properties of MoO3 nanoparticles

A novel photocatalytic activity of Bi2S3 nanoparticles for pharmaceutical and organic pollution removal in water remediation

In recent times, there has been a surge of interest in the unique characteristics and possible applications of nanoparticles in various fields, including environmental clean-up. The contamination of water sources with pharmaceutical and organic substances has emerged as a significant environmental concern. Semiconductor nanoparticles, particularly Bi2S3 nanoparticles, have garnered considerable attention due to their efficacy and environmentally friendly nature in advanced oxidation processes (AOPs). These nanoparticles have shown remarkable photocatalytic abilities in the destruction of pollutants when exposed to UV–visible light. This study covers the production, characterization, and degradation of Bi2S3 nanoparticles in relation to organic and pharmaceutical contaminants. By utilizing co-precipitate methods, it becomes possible to precisely manipulate the size, shape, and composition of the nanoparticles, which are crucial factors in determining their catalytic effectiveness. The created nanoparticles are then examined using various analytical techniques, such as Powder X-ray Diffraction (P-XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), UV-visible spectroscopy, Photoluminescence study (PL), colour chromaticity coordinates (CIE), and Dynamic light scattering study (DLS). The catalytic capability of the synthesised compounds was compared to the degradation of Picric acid and paracetamol as model pollutants. The observed photocatalytic activity of these nanoparticles indicates their potential in water purification and environmental clean-up. The findings suggest that incorporating these nanoparticles into practical systems for pollutant degradation is feasible and can be applied in diverse scenarios. The results shed light on the efficient utilization of Bi2S3 nanoparticles as photocatalysts for the oxidation of drugs and organic contaminants. By utilizing Bi2S3 nanoparticles, the elimination of pharmaceutical and organic pollutants from water sources becomes achievable, thereby contributing to the preservation and safeguarding of the environment and public health.

Macrophages as carriers of boron carbide nanoparticles dedicated to boron neutron capture therapy

BackgroundThe use of cells as carriers for the delivery of nanoparticles is a promising approach in anticancer therapy, mainly due to their natural properties, such as biocompatibility and non-immunogenicity. Cellular carriers prevent the rapid degradation of nanoparticles, improve their distribution, reduce cytotoxicity and ensure selective delivery to the tumor microenvironment. Therefore, we propose the use of phagocytic cells as boron carbide nanoparticle carriers for boron delivery to the tumor microenvironment in boron neutron capture therapy.ResultsMacrophages originating from cell lines and bone marrow showed a greater ability to interact with boron carbide (B4C) than dendritic cells, especially the preparation containing larger nanoparticles (B4C 2). Consequently, B4C 2 caused greater toxicity and induced the secretion of pro-inflammatory cytokines by these cells. However, migration assays demonstrated that macrophages loaded with B4C 1 migrated more efficiently than with B4C 2. Therefore, smaller nanoparticles (B4C 1) with lower toxicity but similar ability to activate macrophages proved to be more attractive.ConclusionsMacrophages could be promising cellular carriers for boron carbide nanoparticle delivery, especially B4C 1 to the tumor microenvironment and thus prospective use in boron neutron capture therapy.Graphical

An Injectable Hydrogel System with Mild Photothermal Effects Combined with Ion Release for Osteosarcoma‐Related Bone Defect Repair

AbstractOsteosarcoma, a common invasive malignant bone disease, presents therapeutic challenges due to the persistent problem of incomplete resection during surgical treatment. This often results in postoperative tumor recurrence and metastasis, and large‐scale bone defects are difficult to self‐repair, seriously affecting patient health. In this study, a dual‐ion doped organic‐inorganic composited SOH1(CP)1 injectable hydrogel system is successfully designed and constructed. This system consists of sericin protein grafted with hydrazide bonds, oxidized chondroitin sulfate, Se and Mg co‐doped HAp nanorods, and polydopamine‐coated CaO2 nanospheres. The system displays strong anti‐tumor activity due to its mild photothermal effects combined with the chemotherapeutic efficacy of SeO32−. Because the degradation behavior of hydrogel matches the bone repair cycle, including the nutritional support of hydrogel skeleton degradation products to promote bone cell proliferation, and the positive regulation of Ca2+, Mg2+, and PO43− released via the degradation of inorganic nanoparticles to promote bone differentiation, the system shows excellent bone defect repair efficacy. Importantly, this system achieves 100% tumor inhibition after 18 days, while ensuring complete bone repair after 12 weeks. Hence, the SOH1(CP)1 injectable hydrogel system, which displays both high anti‐osteosarcoma efficacy and strong bone repair properties, can serve as a new tool for osteosarcoma‐related bone defect repair.

Using the IL-TEM Technique to Understand the Mechanism and Improve the Durability of Platinum Cathode Catalysts for Proton-Exchange Membrane Fuel Cells.

Proton-exchange membrane fuel cells are one of the most promising energy conversion technologies for both automotive and stationary applications. Scientists are testing a number of solutions to increase the durability of cells, especially catalysts, which are the most expensive component. These solutions include, among others, the modification of the composition and morphology of supported nanoparticles, the platinum-support interface, and the support itself. A detailed understanding of the mechanism of platinum degradation and the subsequent improvement of the durability of the entire cell requires the development of methods for effectively monitoring the behavior of catalytic nanoparticles under various cell operating conditions. The Identical-Location Transmission Electron Microscopy (IL-TEM) method makes it possible to visually track structural and morphological changes in the catalyst directly. Because the tests are performed with a liquid electrolyte imitating a membrane, they provide better control of the degradation conditions and, consequently, facilitate the understanding of nanoparticle degradation processes in various operating conditions. This review is primarily intended to disseminate knowledge about this technique to scientists using electron microscopy in the study of energy materials and to draw attention to issues related to the characterization of the structure of carbon supports.

Dual-Responsive Nanogels with Cascaded Gentamicin Release and Lysosomal Escape to Combat Intracellular Small Colony Variants for Peritonitis and Sepsis Therapies.

Intracellular bacteria are the major cause of serious infections including sepsis and peritonitis, but face great challenges in fighting against the stubborn intracellular small colony variants (SCVs). Herein, the authors have developed nanogels (NGs) to destroy both planktonic bacteria and SCVs and eliminate excessive inflammations for peritonitis and sepsis therapies. Free gentamicin (GEN) and hydroxyapatite nanoparticles (NPs) with GEN loading and mannose grafts (mHAG) are inoculated into ε-polylysine NGs to obtain NG@G1-mHAG2 through crosslinking with phenylboronic acid and tannic acid. The H2O2 consumption after reaction with phenylboronic esters and the elimination of free radicals by tannic acid alleviates the escalated inflammatory status to promote sepsis therapy. After mannose-mediated uptake into macrophages, the acid-triggered degradation of mHAG NPs generates Ca2+ to destabilize lysosomes and the efficient lysosomal escape leads to reversion of hypometabolic SCVs into normal phenotype and their sensitivity to GEN. In a peritonitis mouse model, NG@G1-mHAG2 treatment provides strong and persistent bactericidal effects against both extracellular bacteria and intracellular SCVs and extends survival of peritonitis mice without apparent hepatomegaly, splenomegaly, pulmonary edema, and inflammatory cell infiltration. Thus, this study demonstrates a concise and versatile strategy to eliminate SCVs and relieve inflammatory storms for peritonitis and sepsis therapies without infection recurrence.