The present study investigates the analysis and optimization processes of crushed Goat Dung (GD) as a potential co substrate in bioremediation of crude oil contaminated soil (COCS). The first order crude oil (CO) degradation kinetics and biological half-life with the effects of process parameters (pH and initial CO concentration) were also investigated. Results obtained from the effects of process parameters showed that 70 and 75% of CO were degraded at pH 7 and initial CO concentration of 130 g/l. The first order CO degradation kinetics and half-life indicated that the highest CO degradation rate constant and lowest half-life was obtained at 130 g/l. The optimum values of the independent variables as indicated by response surface methodology (RSM) were pH (6.8), temperature (39.5 °C) and CO concentration (85.2 g/l). Good relation between the R2 (98.60%), adjusted R2 (97.05%) and predicted R2 (90.88%) indicated the satisfactory performance of the second order quadratic model. The findings of this present work suggest that GD could be an effective CO substrate in bioremediation of COCS.