Platelet-rich plasma (PRP) is an innovative approach in the management of cartilage and tendon lesions. It is defined as an autologous blood product with a platelet concentration higher than peripheral blood. The application of PRP on damaged sites could promote tissue regeneration because platelets are a source of several growth factors and other bioactive molecules which may play an important role in tissue homeostasis and healing process. PRP has been applied in different pathologies, both as a conservative injective approach or as “augmentation” during surgical procedures. Studies have been published concerning the treatment of Achilles, patellar, and elbow tendinopathy, as well as rotator cuff lesions and cartilage degeneration of knee, hip and talar osteochondral lesions. The aim of the present manuscript is to review the available scientific evidence regarding clinical application of PRP in treating cartilage and tendon disorders.