The maximum deflection and girder-end rotation of the stiffening girder for a suspension bridge are key stiffness indicators that need to be verified to secure the structure's performance during the operation. The overall vertical stiffness of a three-tower suspension bridge is significantly reduced due to the mid-tower being constrained in the horizontal direction of the main cable in the main spans with large sag-to-span ratios. Therefore, rapid and accurate evaluation of the vertical stiffness of the three-tower suspension bridge is quite problematic. Based on the deflection theory, this study proposed an analytical expression of the deflection and girder-end rotation of a three-tower suspension bridge. The deflection and girder-end rotation were used as the optimization objectives. Combining the harmony search algorithm with the “death-penalty” function approach, the most unfavorable loading conditions and corresponding maximum values were obtained. The effectiveness of the proposed method was verified by finite element modeling of an exemplary-three-tower suspension bridge. The proposed method had high accuracy, improved calculation efficiency, and wide applicability, which made it lucrative for selecting the optimal structural parameters in the preliminary design of suspension bridges. The dead-to-live load ratio, cable sag-to-span ratio, mid-tower lateral stiffness, side-to-main span ratios, cable’s axial stiffness, and deck’s flexural rigidity were further analyzed. The results indicated that increasing the dead-to-live load ratio and mid-tower stiffness was the most effective method to control maximum deflection and girder-end rotation of stiffening girders.
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