Secondary metabolites, such as phenolics, are plant defence substances. In the present study, the impact of Monilinia laxa inoculation under controlled conditions on phenolic content of apricot branches was investigated. A bi-parental hybrid population issued from Bergeron and Bakour cultivars (BerBa) and consisting of 192 hybrids was studied. The susceptibility of the BerBa population to M. laxa was evaluated by measuring the length of the necrosed tissues and the concentration of fungal DNA by qPCR in the branches 8 days post-inoculation. The results exhibit significant differences between the two parental cultivars in terms of necrosis length, which confirm their different susceptibility to the pathogen. A considerably high content of 2′,6′-dihydroxy-4′–methoxyacetophenone hexoside was found in branch tissues of Bakour (tolerant parental cultivar), amounting to 1.72 mg/g and 1.41 mg/g of fresh weight in non-inoculated and inoculated samples, respectively. The content of this compound in Bergeron branch tissues was several times lower, amounting to 0.24 mg/g and 0.14 mg/g of fresh weight in non-inoculated and inoculated samples, respectively. In the inoculated branches, scopolin was almost twice as abundant in the tolerant parental cultivar branch tissues as in the susceptible ones. In general, after inoculation a lower content of phenolic compounds was observed for hybrids with longer necrosed tissue. A Principal Component Analysis showed that at 8 days post-inoculation M. laxa concentration in the branches and the content of phenolic compounds, such as scopolin and chlorogenic acids, were negatively correlated. Additionally, the antifungal activity of pure phenolic compounds against M. laxa mycelial growth and spore germination was investigated. At a concentration of 500 mg/L, up to 40 % inhibition of M. laxa mycelial growth by scopoletin, up to 60 % inhibition by 2′,4′,6′-trihydroxyacetophenone and total inhibition by 2′-hydroxy-4′-methoxyacetophenone was observed. Scopoletin and 2′,4′,6′-trihydroxyacetophenone also inhibited spore germination by about 50 %. These results can serve to improve breeding programs aiming to develop apricot cultivars resistant to brown rot blossom blight.