Corrigendum6 July 2011free access Functional roles for evolutionarily conserved Spt4p at centromeres and heterochromatin in Saccharomyces cerevisiae Luciana B Crotti Luciana B Crotti Search for more papers by this author Munira A Basrai Munira A Basrai Search for more papers by this author Luciana B Crotti Luciana B Crotti Search for more papers by this author Munira A Basrai Munira A Basrai Search for more papers by this author Author Information Luciana B Crotti and Munira A Basrai The EMBO Journal (2011)30:2749-2749https://doi.org/10.1038/emboj.2011.167 This article corrects the following: Functional roles for evolutionarily conserved Spt4p at centromeres and heterochromatin in Saccharomyces cerevisiae01 April 2004 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Correspondence to: [email protected] Correction to: The EMBO Journall (2004) 23, 1804–1814. doi:10.1038/sj.emboj.7600161 Upon examination of the laboratory notebook the authors of the paper report that wrong versions of Figure 2 and Figure 6A and B were submitted for publication. They would like to replace these figures and wish to emphasize that this does not affect the conclusions of the paper. Figure 1.Ndc10p is required for the association of HA-Spt4p to the core CEN loci. ChIP experiments were carried out using wild-type strain (GHY262) or ndc10-1 strain expressing HASPT4 (YMB1659) grown at 30 or 25°C and shifted to 37°C for 4 h. (A, B) Chromatin samples from T, HA, and M were analysed using primers for core CEN16, TELVIR, or ACT1. (C) Chromatin samples from T (row 1), HA (rows 2 and 3), and M (row 3) were analysed using primers for core CEN16 (lane 4) and those that flank on either side (lanes 1–3 and 5–6). Download figure Download PowerPoint Figure 2.RNAPII and Sir3p are not required for the association of HA-Spt4p to core CEN and spt4 strains display a defect in silencing of reporter genes at heterochromatic loci. (A) HA-Spt4p associates with CEN DNA, but not with the actively transcribed gene TUP1 in a temperature-sensitive rpb1-1 strain. ChIP experiments were carried out using wild-type (GHY501) and rpb1-1 (GHY560) strains expressing HASPT4 (pMB237) grown at 30°C and shifted to 37°C for 1 h. Chromatin samples from total (T), immunoprecipitated with anti-HA (HA), and mock (M) were analysed using primers for core CEN1 or TUP1. (B) HA-Spt4p associates with core CEN DNA, but not with HMRa and TEL in a sir3Δ strain. ChIP experiments were carried out using wild-type (BUY668) and sir3Δ (BUY671) strains expressing HASPT4 (pMB237) grown at 30°C. Chromatin samples from T, HA, and M were analysed using primers for core CEN1, HMRa, TELVIR, or ACT1. These results suggest that Spt4p may not be required for the transcription of HMRa and TELVIR. Download figure Download PowerPoint Previous ArticleNext Article Read MoreAbout the coverClose modalView large imageVolume 30,Issue 13,July 6, 2011“Chromatography” - This photograph was taken by Bruno Guy, a research immunologist at sanofi pasteur, France. He describes the abstract shapes and colours of the image as “the shadows of strings in winter - a short-lived chromatography record”. Volume 30Issue 136 July 2011In this issue FiguresRelatedDetailsLoading ...