Oxide Defects Research Articles

Oxide and Interface Defect Analysis of lateral 4H-SiC MOSFETs through CV Characterization and TCAD Simulations

We investigated oxide and interface defects of lateral 4H-SiC MOSFETs through capacitance-voltage (C-V) and conductance-voltage (G-V) characterization at various frequencies and temperatures. By employing consecutive up and down sweeps of the gate voltage at three different temperatures, we experimentally characterized the hysteresis width as the difference between up and down sweeps in the depletion to accumulation (d-a) and depletion to inversion (d-i) regions. We observed an increase in the hysteresis width with decreasing temperature. Although the hysteresis width is not affected by the small-signal frequency, at the same time, increasing the frequency leads to a strong stretch-out effect, especially in the d-i region.Our measurement results indicate that the hysteresis deformation of the C-V curves is dominated by three different trap types. First, interface acceptor-like defects located close to the conduction band can follow the small-signal frequency. Slower acceptor-like border traps with trap levels both close to the conduction band and in the middle of the band gap are however responsible for the increase of trapped negative charge with increasing gate voltage. Finally, we assume the presence of a fixed positive charge.

The Influence of Extended Defects in 4H-SiC Epitaxial Layers on Gate Oxide Performance and Reliability

For the ongoing commercialization of power devices based on 4H-SiC, increasing the yield and improving the reliability of these devices is becoming more and more important. In this investigation, gate oxide on 4H-SiC was examined by time-zero dielectric breakdown (TZDB) and constant current stress (CCS) time-dependent dielectric breakdown (TDDB) method in order to get insights into the influence of the epitaxial defects on the gate oxide performance and reliability. For that purpose, MOS capacitors with different gate oxides have been fabricated. Crystal defects in the epitaxial layers have been detected and mapped by ultraviolet photoluminescence (UVPL) and interference contrast (DIC) imaging. The results of the comparison of electrical data and surface mapping data indicate a negative influence on the leakage current behavior for some extended epitaxial defects. Results from TDDB measurement indicated numerous extrinsic defects, which can be traced back to gate oxide processing conditions and defect densities.

Universal Strategy for Reversing Aging and Defects in Graphene Oxide for Highly Conductive Graphene Aerogels.

The production of highly stable, defect-free, and electrically conducting 3D graphene structures from graphene oxide precursors is challenging. This is because graphene oxide is a metastable material whose structure and chemistry evolve due to aging. Aging changes the relative composition of oxygen functional groups attached to the graphene oxide and negatively impacts the fabrication and properties of reduced graphene oxide. Here, we report a universal strategy to reverse the aging of graphene oxide precursors using oxygen plasma treatment. This treatment decreases the size of graphene oxide flakes and restores negative zeta potential and suspension stability in water, enabling the fabrication of compact and mechanically stable graphene aerogels using hydrothermal synthesis. Moreover, we employ high-temperature annealing to remove oxygen-containing functionalities and repair the lattice defects in reduced graphene oxide. This method allows obtaining highly electrically conducting graphene aerogels with electrical conductivity of 390 S/m and low defect density. The role of carboxyl, hydroxyl, epoxide, and ketonic oxygen species is thoroughly investigated using X-ray photoelectron and Raman spectroscopies. Our study provides unique insight into the chemical transformations occurring during the aging and thermal reduction of graphene oxide from room temperature up to 2700 °C.

Research of surface oxidation defects in copper alloy wire arc additive manufacturing based on time-frequency analysis and deep learning method

In this paper a novel method was proposed for monitoring the defects of surface oxidation in copper alloy wire arc additive manufacturing (WAAM) which using voltage sensor. Due to the good fluidity of liquid copper alloy, the dimension of copper alloy WAAM process is difficult to control especially in the height direction. This will lead to error accumulation with the layers increasing during WAAM period. The phenomenon of error accumulation will cause continuous changes in the distance between the welding wire and substrate, result in inappropriate gas protection which will lead to surface oxidation defects happen. To monitoring this defect，time-frequency analysis method was used to compare the voltage signals between normal and anomaly WAAM process. The result show that the voltage signal of abnormal weld bead appears fluctuations in its peak value, and the voltage waveform of the normal WAAM process is relatively smooth. Furthermore, continuous wavelet transform (CWT) method is used to convert one-dimensional time-series voltage signals into two-dimensional time-frequency images, which is combined with deep learning method to establish an online monitoring model to monitor the surface oxidation defects in the process of copper alloy WAAM. The accuracy of the proposed model can reach 95.83%, and t-SNE(t-distributed stochastic neighbor embedding) algorithm is used to visualize the performance of the model. The result indicates that the voltage signal characteristics of normal and abnormal WAAM processes were significantly distinguished.

Failure Mechanism and Optimization of Metal-Supported Solid Oxide Fuel Cells.

A solid oxide fuel cell (SOFC) is a clean, efficient energy conversion device with wide fuel applicability. Metal-supported solid oxide fuel cells (MS-SOFCs) exhibit better thermal shock resistance, better machinability, and faster startup than traditional SOFCs, making them more suitable for commercial applications, especially in mobile transportation. However, many challenges remain that hinder the development and application of MS-SOFCs. High temperature may accelerate these challenges. In this paper, the existing problems in MS-SOFCs, including high-temperature oxidation, cationic interdiffusion, thermal matching, and electrolyte defects, as well as lower temperature preparation technologies, including the infiltration method, spraying method, and sintering aids method, are summarized from different perspectives, and the improvement strategy of existing material structure optimization and technology integration is put forward.

Diagnosis and management of metabolic myopathies.

Metabolic myopathies are a set of rare inborn errors of metabolism leading to disruption in energy production. Relevant to skeletal muscle, glycogen storage disease and fatty acid oxidation defects can lead to exercise intolerance, rhabdomyolysis, and weakness in children and adults, distinct from the severe forms that involve multiple-organ systems. These nonspecific, dynamic symptoms along with conditions that mimic metabolic myopathies can make diagnosis challenging. Clinicians can shorten the time to diagnosis by recognizing the typical clinical phenotypes and performing next generation sequencing. With improved access and affordability of molecular testing, clinicians need to be well-versed in resolving variants of uncertain significance relevant to metabolic myopathies. Once identified, patients can improve quality of life, safely engage in exercise, and reduce episodes of rhabdomyolysis by modifying diet and lifestyle habits.

Serum metabolomic signatures of fatty acid oxidation defects differentiate host-response subphenotypes of acute respiratory distress syndrome

BackgroundFatty acid oxidation (FAO) defects have been implicated in experimental models of acute lung injury and associated with poor outcomes in critical illness. In this study, we examined acylcarnitine profiles and 3-methylhistidine as markers of FAO defects and skeletal muscle catabolism, respectively, in patients with acute respiratory failure. We determined whether these metabolites were associated with host-response ARDS subphenotypes, inflammatory biomarkers, and clinical outcomes in acute respiratory failure.MethodsIn a nested case–control cohort study, we performed targeted analysis of serum metabolites of patients intubated for airway protection (airway controls), Class 1 (hypoinflammatory), and Class 2 (hyperinflammatory) ARDS patients (N = 50 per group) during early initiation of mechanical ventilation. Relative amounts were quantified by liquid chromatography high resolution mass spectrometry using isotope-labeled standards and analyzed with plasma biomarkers and clinical data.ResultsOf the acylcarnitines analyzed, octanoylcarnitine levels were twofold increased in Class 2 ARDS relative to Class 1 ARDS or airway controls (P = 0.0004 and < 0.0001, respectively) and was positively associated with Class 2 by quantile g-computation analysis (P = 0.004). In addition, acetylcarnitine and 3-methylhistidine were increased in Class 2 relative to Class 1 and positively correlated with inflammatory biomarkers. In all patients within the study with acute respiratory failure, increased 3-methylhistidine was observed in non-survivors at 30 days (P = 0.0018), while octanoylcarnitine was increased in patients requiring vasopressor support but not in non-survivors (P = 0.0001 and P = 0.28, respectively).ConclusionsThis study demonstrates that increased levels of acetylcarnitine, octanoylcarnitine, and 3-methylhistidine distinguish Class 2 from Class 1 ARDS patients and airway controls. Octanoylcarnitine and 3-methylhistidine were associated with poor outcomes in patients with acute respiratory failure across the cohort independent of etiology or host-response subphenotype. These findings suggest a role for serum metabolites as biomarkers in ARDS and poor outcomes in critically ill patients early in the clinical course.

Synthesis of chemically exfoliated reduced graphene oxide from as-grated graphite rods recovered from used household batteries for utilization in tribological applications

The research objective of this work is to reuse graphite rods recovered from used batteries and utilize them as an additive in SAE20W40 oil to improve the engine parts’ lubrication ability and wear resistance. The graphite rods are grated to obtain ultra-fine particles and then chemically exfoliated using an Improved Hummer's process that results in graphene oxide. Then, it is thermally reduced at 350 °C to yield reduced graphene oxide. Different ratios (0.25, 0.50, 0.75, and 1.00 wt.%) of as-grated graphite particles and reduced graphene oxide were dispersed in the above oil to form a colloidal solution and then used for tribological studies. The graphite particles and synthesized reduced graphene oxide were characterized using X-ray diffraction, Raman spectroscopy, and high-resolution transmission electron microscopy. X-ray diffraction data confirms the presence of graphite, graphene oxide, and reduced graphene oxide in the prepared powders and matches with the standard files. High-resolution transmission electron microscopy images reveal the presence of multilayer sheets with an interplanar distance of 0.34 nm having wrinkles, folds, and gas holes. The G and D band positions of Raman spectra prove the formation of oxygenated functional groups, and the ID/ IG ratio confirms the defects in graphene oxide and reduced graphene oxide. The viscosity of prepared lubricant increases by 35% and 21% for graphite and reduced graphene oxide, respectively. The results of tribological studies with 0.25 wt.% graphite and 0.5 wt.% reduced graphene oxide lubricants showed improvement in coefficient of friction by 32% and 36%, respectively. At these optimal concentrations, the specific wear rate dropped by 51% for reduced graphene oxide. Scanning electron microscopy/energy dispersive spectroscopy, images show that the nanolubricant's mending effect over the worn surface improves reduced graphene oxide added SAE20W40 oil tribological characteristics. From the results, reduced graphene oxide synthesized from as-grated graphite rods proves to be a low-cost, sustainable, and effective friction modifier for engine oils.

Response Mechanisms of Additional Displacement Defects in Oxides to Ionization Damage in Bipolar Transistors

When evaluating the radiation damage of charged particles to bipolar junction transistors (BJTs), the additional displacement defects caused by charged particles in oxide cannot be ignored. In this paper, we demonstrate that the additional displacement defects can enhance or weaken the ionization damage level of BJTs. Additional displacement defects always weaken ionization damage in PNP BJTs and may enhance it in NPN BJTs. In addition, in the same BJTs, the response of additional displacement defects in oxide at different depths to ionization damage is also different, which strongly depends on the depth of additional displacement defects. Additional displacement defects in oxide show different results in response to ionization damage in different types of BJTs, which brings complexity to the accurate assessment of radiation hardness of BJTs. Therefore, in this paper, three different types of BJTs are analyzed in detail. In their oxides, the response mechanism of additional displacement defects to ionization damage is analyzed in detail, which provides a reference for improving the evaluation accuracy of more types of BJTs.

Abnormal Dihydrorhodamine Result in a Patient with Diabetes Mellitus, Recurrent Candida Liver Abscesses: Diagnostic Possibilities

A 17-year-old female with type I diabetes mellitus (DM) and a history of recurrent candidal vaginal infections and liver abscesses was assessed fora potential neutrophil oxidative burst defect by DHR flow cytometry. The DHR result was abnormal with significantly decreased MFI (MFI = 9; ref. cutoff: >/ = 60) after stimulation with PMA though all of her neutrophils (99%; ref. cutoff >/ = 95%) showed respiratory burst. This DHR pattern has been previously observed in patients with complete myeloperoxidase deficiency (cMPO), p40phox deficiency and hypomorphic X-linked chronic granulomatous disease (CGD). Genetic evaluation of the genes commonly associated with CGD (CYBB, CYBA, NCF1, NCF2 and NCF4) was negative. It must be noted that many genetic laboratories either do not sequence the NCF1 gene or have limited sequencing to the most commonly observed pathogenic variant in exon 2. Most targeted genetic panels currently do not include MPO (myeloperoxidase) or CYBC1 (Eros). The clinical phenotype of Candida infection in the context of diabetes has been previously reported in 5% of cMPO deficiency. The patient underwent a partial hepatectomy and was initiated on an 8-month course of fluconazole. Three months later, there was a recurrence of hepatic abscesses (Figure 1), requiring treatment with a 12-week course of IV micafungin. Complete MPO deficiency can cause a false-positive result on the DHR assay but the pattern is different from that observed in typical XL- and autosomal recessive CGD. There are two ways of confirming cMPO deficiency, besides genetic testing: 1) staining for MPO in neutrophils in a peripheral smear, or 2) repeating the DHR assay and gating on eosinophils as eosinophil peroxidase can overcome the lack of MPO deficiency and demonstrate a normal oxidative burst. These studies are currently under investigation. In summary, cMPO deficiency should be included in the differential diagnosis of patients with diabetes and invasive Candida infections. Communication with the clinical laboratory is essential to identify the DHR pattern and obtain additional confirmatory testing. From a clinical perspective, cMPO-deficient patients do not require prophylactic antibiotics but they have a high rate of recurrence of fungal infections and require aggressive management with antifungal therapies. [Display omitted]

The age-related changes in lymph node stromal cells underlie defects in peripheral T cell maintenance and immune function decline in old mice.

Abstract Older adults are more vulnerable to infection and cancer, and poorly respond to vaccination. The structural stromal cells in the lymph nodes (LN) are essential regulators of T cell homeostasis and immune response. However, how does aging of LN stromal cells contribute to T cell maintenance defects and functional immunity is incompletely understood. Here we used single cell RNA-Seq of purified stromal cells from adult (3mo) and old (21mo) peripheral LN of C57BL/6 mice to characterize the age-related transcriptional differences in major stromal cell subsets. Our data revealed the enrichment of CCL19 hiand CCL19 lowwhile diminution of Nr4a1 +and Inmt +FRC in old LN. The enriched FRC in old LN exhibited a gene signature of cellular and oxidative stress response, mitochondrial defects, and negative regulation of immunity. The ex vivo functional characterization revealed that old LN stromal cells exhibit elevated mitochondrial reactive oxygen species coupled with increased mitochondrial mass and reduced membrane potential, indicative of mitochondrial defects during the senescence. Interestingly, these defects are observed in older but not mid-age LN. Furthermore, in vitro co-culture of LN stromal cells and purified naive T (T N) cells showed that old LN stromal cells poorly support CD8 T Ncell survival. Finally, boosting the function of old LN stromal cells using agonistic anti-LTbR or mitigating oxidative stress using N-Acetyl Cysteine reversed this effect, via stabilization of BCL2 in T Ncells. Thus, these results suggest that age-related adverse changes in LN stromal cells contribute to defects in maintenance of T Ncells, and highlight that these defects could be reversed to restore T Ncell homeostasis and functional immunity. This work is supported by grants from NIH (P01 AG052359 and R37 AG020719) to Dr. Janko Nikolich-Zugich.

Operando Interaction and Transformation of Metastable Defects in Layered Oxides for Na‐Ion Batteries

AbstractNon‐equilibrium defects often dictate the macroscopic properties of materials. They largely define the reversibility and kinetics of processes in intercalation hosts in rechargeable batteries. Recently, imaging methods have demonstrated that transient dislocations briefly appear in intercalation hosts during ion diffusion. Despite new discoveries, the understanding of impact, formation and self‐healing mechanisms of transient defects, including and beyond dislocations, is lacking. Here, operando X‐ray Bragg Coherent Diffractive Imaging (BCDI) and diffraction peak analysis capture the stages of formation of a unique metastable domain boundary, defect self‐healing, and resolve the local impact of defects on ionic diffusion in NaxNi1−yMnyO2 intercalation hosts in a charging sodium‐ion battery. Results, applicable to a wide range of layered intercalation materials due to the shared nature of framework layers, elucidate new dynamics of transient defects and their connection to macroscopic properties, and suggest how to control the nanostructure dynamics.

Raspberry Extract as a Strategy to Improve the Oxidative Stability of Pork Burgers Enriched with Omega-3 Fatty Acids.

Hydrogelled emulsions (HEs) of linseed oil and pea protein (PP) were produced with four levels (0, 5, 7.5, and 10%) of raspberry extract obtained by a green extraction technique (microwave hydrodiffusion and gravity-MHG). HEs were applied in burgers to replace 50% of pork backfat content. The products' technological, nutritional, oxidative, microbiological, and sensory properties were evaluated. Besides reducing the fat level by approximately 43%, the reformulation reduced the n-6/n-3 PUFA ratio to healthy levels, decreased the diameter reduction by 30%, and increased the cooking yield by 11%. Including 7.5 and 10% of raspberry extract in the HEs decreased the oxidative defects caused by the enrichment of the burgers with omega-3 fatty acids. In addition, the raspberry extract did not cause alterations in the mesophilic aerobic count and the burgers' sensory profile.

Why Don’t More Mitochondrial Diseases Exhibit Cardiomyopathy?

Although the heart requires abundant energy, only 20-40% of children with mitochondrial diseases have cardiomyopathies. We looked for differences in genes underlying mitochondrial diseases that do versus do not cause cardiomyopathy using the comprehensive Mitochondrial Disease Genes Compendium. Mining additional online resources, we further investigated possible energy deficits caused by non-oxidative phosphorylation (OXPHOS) genes associated with cardiomyopathy, probed the number of amino acids and protein interactors as surrogates for OXPHOS protein cardiac "importance", and identified mouse models for mitochondrial genes. A total of 107/241 (44%) mitochondrial genes was associated with cardiomyopathy; the highest proportion were OXPHOS genes (46%). OXPHOS (p = 0.001) and fatty acid oxidation (p = 0.009) defects were significantly associated with cardiomyopathy. Notably, 39/58 (67%) non-OXPHOS genes associated with cardiomyopathy were linked to defects in aerobic respiration. Larger OXPHOS proteins were associated with cardiomyopathy (p < 0.05). Mouse models exhibiting cardiomyopathy were found for 52/241 mitochondrial genes, shedding additional insights into biological mechanisms. While energy generation is strongly associated with cardiomyopathy in mitochondrial diseases, many energy generation defects are not linked to cardiomyopathy. The inconsistent link between mitochondrial disease and cardiomyopathy is likely to be multifactorial and includes tissue-specific expression, incomplete clinical data, and genetic background differences.

Reconstructed anti-poisoning surface for enhanced electrochemical CO2 reduction on Cu-incorporated ZnO

Zn-based materials are regarded as a family of promising electrocatalysts for electrochemical CO2 reduction reaction (e-CO2RR), especially for the CO2-to-CO conversion. However, their electrocatalytic performances should be improved and the conversion mechanism needs to be further studied. In this work, we fabricate Cu-incorporated ZnO (Cu25Zn-A) on Zn plate for e-CO2RR catalyst by a facile annealing method. We find that Cu25Zn-A achieves a high CO Faraday efficiency > 90% with a CO yield rate of 0.49 mmol cm−2 h−1. The improved catalytic activity on Cu25Zn-A is mainly attributed to the Cu steps on the reconstructed surface in the reaction: (1) that weakens the OH-/CO32- adsorption, leading to the anti-poisoning surface by preventing the formation of Zn hydroxide/carbonates; and (2) that enhances the adsorption/activation of reactants and stabilizes the intermediates. Additionally, the density of defects in Zn oxide is increased by the Cu-incorporation, resulting in improved deoxygenation step. Our findings may provide insightful understanding on the mechanism and guide the design of novel electrocatalyst for effective CO2 reduction.

Impact of Temperature-Induced Oxide Defects on Hf x Zr1−xO2 Ferroelectric Tunnel Junction Memristor Performance

In this work, we evaluate the importance of the postmetallization annealing (PMA) temperature on the performance of HfxZr1−xO2-based ferroelectric tunnel junctions (FTJs). Our results indicate a significant difference in tunneling electroresistance (TER) ratio and endurance, depending on the PMA temperature despite negligible variations in remanent polarization. We conclude that the minimization of conductive oxide defect states is central to achieve high performance. Through carefully optimized PMA conditions, we demonstrate FTJs with a TER = 3 and low mean cycle-to-cycle variation of < 1.5% combined with at least 16 separable conductance states providing a 4-bit resolution analog FTJ.

Molten salt-lithium process induced controllable surface defects in titanium oxide for efficient photocatalysis

The efficiency of photoabsorption, photo-generated charge separation, and surface redox reaction determine the overall efficiency of photocatalysts. Therefore, exploring ways to simultaneously optimize the parameters is key to improving the photocatalytic performance. Herein, a novel low-temperature ternary molten salt-lithium reduction method is designed to create controllable oxygen vacancies (Ovs) as well as to manipulate the surface microstructure of the classic photocatalyst TiO2. The optimized TiO2 exhibits a 10-fold increase in the photocatalytic RhB breakdown rate and H2 generation quantity compared to pristine TiO2. The dual surface defects result in synergistic effects: i) Ovs lower band gap, enhance the charge separation efficiency as capture centers, and facilitate hydrogen adsorption; ii) the enlarged surface area enhances light-harvesting and provides more active sites. This research proposes a novel strategy for manipulating surface defects in a controlled manner and highlights the synergistic optimization of the thermodynamical and kinetical parameters to promote the photocatalytic performance.

Differentiating between Acidic and Basic Surface Hydroxyls on Metal Oxides by Fluoride Substitution: A Case Study on Blue TiO2 from Laser Defect Engineering.

Both oxygen vacancies and surface hydroxyls play a crucial role in catalysis. Yet, their relationship is not often explored. Herein, we prepare two series of TiO2 (rutile and P25) with increasing oxygen deficiency and Ti3+ concentration by pulsed laser defect engineering in liquid (PUDEL), and selectively quantify the acidic and basic surface OH by fluoride substitution. As indicated by EPR spectroscopy, the laser-generated Ti3+ exist near the surface of rutile, but appear to be deeper in the bulk for P25. Fluoride substitution shows that extra acidic bridging OH are selectively created on rutile, while the surface OH density remains constant for P25. These observations suggest near-surface Ti3+ are highly related to surface bridging OH, presumably the former increasing the electron density of the bridging oxygen to form more of the latter. We anticipate that fluoride substitution will enable better characterization of surface OH and its correlation with defects in metal oxides.

A Brief Review of Sodium Bismuth Titanate-Based Lead-Free Materials for Energy Storage: Solid Solution Modification, Metal/metallic Oxide Doping, Defect Engineering and Process Optimizing

With the ever-increasing demand for energy, research on energy storage materials is imperative. Thereinto, dielectric materials are regarded as one of the potential candidates for application in advanced pulsed capacitors by reason of their ultrahigh energy-storage density, low energy loss, and good thermal stability. Among the numerous dielectric materials for energy storage, sodium bismuth titanate (Bi0.5Na0.5TiO3, BNT) with high saturation polarization, as one of the successful alternatives to lead-based materials, has been extensively studied. However, degraded dielectric and ferroelectric properties as a consequence of chemical alterations usually produced by inhomogeneity in microstructure and composition due to the ion volatilization during preparing, thus affecting performance of devices. Hence, this review served to encompass the current state and progress on the optimization of energy storage performance in lead-free BNT-based materials over the past few years, including ceramics, multilayer ceramics, thin films, and thick films, involved in solid solution modification, metal/metallic oxide doping, process optimization and other related aspects to optimize energy storage performance. Furthermore, some prospective approach in the improvement of energy storage performance for BNT-based materials were also provided in this work according to the existing theoretical and experimental results, to impel their practical application.

Opposing manner of miR-455-3p against NR2B-PSD-95-nNOS complex in the cortex and hippocampus of depressive rats under simulated complex space environment.

Depression in astronauts is one of the consequences of space flight effects, negatively impacting their work performances. Unfortunately, the underlying molecular mechanisms in space flight-induced depression are still unknown; however, various neuropsychiatric disorders reported that overexpressed NR2B-PSD-95-nNOS complex in the brain triggers various pathological pathways, and inhibiting NR2B-PSD-95-nNOS complex asserts antidepressant effects. Through our in silico analysis, we found that epigenetic regulator miR-445-3p targets PSD-95 and is hypothesized to down-regulate NR2B-PSD-95-nNOS complex to prevent neuronal damage associated with depression. Therefore, the present study is aimed to determine the novel insight of the miR-455-3p against the NR2B-PSD-95-nNOS complex in the neurobiology of space flight-induced depressive behavior. Using a simulated space environment complex model (SCSE) for 21 days, we induced depressive behavior in rats to analyze miR-455-3p expression and NR2B-PSD-95-nNOS complex in the cortex and hippocampus of the SCSE depressed rats through qRT-PCR and western blot analysis. Further, an in vitro microgravity model using rat hippocampus cell lines (RHNC) was utilized to identify the independent role of miR-455-3p on (1) NR2B-PSD-95-nNOS complex and TrKB-BDNF proteins, (2) oxidative stress, (3) nitric oxide level, (4) inflammatory cytokines, (5) mitochondrial biogenesis/ dynamics, and (6) cell survival. Our results showed that miR-455-3p regulates NR2B-PSD-95-nNOS complex in the SCSE depressed rats in opposite ways, with the cortex revealing a higher level of miR-455-3p and low-level NR2B-PSD-95-nNOS complex and the hippocampus showing down-regulated miR-455-3p and up-regulated NR2B-PSD-95-nNOS complex, indicating a region-specific change in the miR-455-3p and NR2B-PSD-95-nNOS complex in the SCSE depressed rats. Further RHNC results also confirmed down-regulated miR-455-3p and up-regulated NR2B-PSD-95-nNOS complex expression, similar to the findings in the hippocampus of SCSE rats, suggesting that microgravity influences miR-455-3p and associated changes. Additional investigations revealed that miR-455-3p targets PSD-95 and co-regulates NR2B-PSD-95-nNOS complex along with TrkB-BDNF signaling and exert protective effects against NR2B-PSD-95-nNOS complex, oxidative stress, nitric oxide, inflammatory cytokines, and mitochondrial defects, suggesting a valuable biomarker for devising depressive disorders.