The currently known Liphistius species of peninsular Malaysia are assigned to five species-groups: the tranggroup, the malayanus-group, the batuensis-group, the tioman-group and the linang-group. The latter four are defined and treated here. They are composed of eleven species. Four of them, L. negara sp. nov., L. gracilis sp. nov., L. priceae sp. nov. and L. linang sp. nov., are new and here described from males and females. Liphistius indra sp. nov., very closely related to L. linang sp. nov., is additionally described from the deep south of Thailand. The previously unknown males of L. endauSedgwick & Platnick, 1987 and of L. tempurung Platnick in Platnick et al., 1997 are presented for the first time. Liphistius malayanus cameroniHaupt, 1983 is placed in the synonymy of L. malayanusAbraham, 1923b. Information and illustrations of intraspecific variation in most species of these groups (except for L. johorePlatnick & Sedgwick, 1984, for which no new material is available) is given together with data on biology and distribution. Taxonomic characters and biogeography are discussed.