Deep-sea Sediments Research Articles

Improved resolution of microbial diversity in deep-sea surface sediments using PacBio long-read 16S rRNA gene sequencing.

The PacBio long-read platform, with its exceptional base-level resolution exceeding 99%, has advanced our comprehension of deep-sea microbial diversity. By comparing microbial community analyses conducted using the Illumina short-read and PacBio long-read sequencing platforms, we have provided an enhanced understanding of fine spatial-scale patterns in microbial community diversity with depth across a deep-sea sediment core, as well as methodological insights that will be valuable for future research in this field.

Bacterial Diversity in Deep-Sea Sediment of West Pacific Nodule Province

Dense polymetallic nodule fields are found in different areas of the Pacific and Indian Oceans. However, limited knowledge exists about microbial diversity, processes and functions in deep-sea polymetallic nodule sediments. This study investigated microbial diversity, composition and function in sediments from various locations and depths in a western Pacific polymetallic nodule province. Sediment cores were collected, DNA extracted, and the V3–V4 regions of the 16S rRNA gene were sequenced using Illumina MiSeq. The test results show that the abundance and diversity of microbial communities in sediments from different sites vary significantly. The dominant microbial communities at the family level in the three sediment samples were all mainly Marinobacteraceae and Alcanivoracaceae. Sediment samples from core 1 had similar microbial structures and microbial community functions. Surface sediment had higher species richness, diversity and evenness than the middle layer. The dominant phylum at different depths was consistent. There was significant spatial heterogeneity in the microbial community within sediments from polymetallic nodule regions. This study expands on our knowledge of the spatial and vertical distribution of microbial community diversity beneath polymetallic nodules in deep-sea settings.

Revealing the metabolic potential and environmental adaptation of nematophagous fungus, Purpureocillium lilacinum, derived from hadal sediment

The extreme environment shapes fungi in deep-sea sediments with novel metabolic capabilities. The ubiquity of fungi in deep-sea habitats supports their significant roles in these ecosystems. However, there is limited research on the metabolic activities and adaptive mechanisms of filamentous fungi in deep-sea ecosystems. In this study, we investigated the biological activities, including antibacterial, antitumor and nematicidal activity of Purpureocillium lilacinum FDZ8Y1, isolated from sediments of the Mariana Trench. A key feature of P. lilacinum FDZ8Y1 was its tolerance to high hydrostatic pressure (HHP), up to 110 MPa. We showed that HHP affected its vegetative growth, development, and production of secondary metabolites, indicating the potential for discovering novel natural products from hadal fungi. Whole-genome sequencing of P. lilacinum FDZ8Y1 revealed the metabolic potential of this piezotolerant fungus in carbon (carbohydrate metabolism), nitrogen (assimilatory nitrate reduction and protein degradation) and sulfur cycling processes (assimilatory sulfate reduction). Transcriptomic analysis under elevated HHP showed that P. lilacinum FDZ8Y1 may activate several metabolic pathways and stress proteins to cope with HHP, including fatty acid metabolism, the antioxidant defense system, the biosynthetic pathway for secondary metabolites, extracellular enzymes and membrane transporters. This study provides valuable insights into the metabolic potential and adaptation mechanisms of hadal fungi to the challenging conditions of the hadal environment.

Calcite Dissolution-Reprecipitation Reactions Are a Key Control on the Sr/Ca, Mg/Ca and δ88/86Sr Compositions of Himalayan River Waters

Silicate weathering on the continents is thought to play a critical part in regulating global climate on geological time scales but determination of the magnitude of silicate weathering fluxes is frustrated by the complexity of the weathering processes. Here we present analyses of stable Sr-isotopic compositions (𝛿88/86Sr) in a suite of river waters and bedloads from the Himalayas in Nepal to establish the lithological controls on 𝛿88/86Sr values and the secondary processes that impact carbonate weathering. A control on 𝛿88/86Sr values is lithology with the rivers in carbonate-dominated catchments marginally lower (∼0.07 ‰) than in silicate-dominated catchments. However, as for 87Sr/86Sr ratios, 𝛿88/86Sr values of carbonates are altered by silicate-carbonate mineral exchange during metamorphism. The major potential secondary control on 𝛿88/86Sr values in Himalayan catchments is precipitation of secondary calcite responsible for the marked elevation of Sr/Ca and Mg/Ca ratios in the waters. We re-evaluate the mechanisms for secondary calcite formation and conclude that a balanced solution and re-precipitation process, driven by the relative instability of the primary Mg-rich calcite, provides a better explanation for the elevated Sr/Ca ratios than simple precipitation from a highly over saturated solution. This solution-re-precipitation process is akin to that invoked to explain diagenesis of deep-sea sediments. The mechanism of secondary calcite formation impacts the distinction of cation inputs from carbonate and silicate minerals. The correlation between 𝛿88/86Sr water-calcite fractionations, Sr/Ca partition coefficients and precipitation rates allows the calcite re-precipitation rates to be inferred from the covariation of water 𝛿88/86Sr values and Sr/Ca ratios. These rates are very low (<10-8 mol m-2 s-1) but are consistent with those inferred from field estimates of the amount of calcite re-precipitated, the surface area of carbonate exposed to weathering and the calcite weathering flux. The low precipitation rates are also consistent with previously reported 𝛥44/40Ca isotope fractionations of ∼−0.2‰. The calcite reprecipitation rates are comparable to silicate weathering rates previously inferred from Li-isotopic compositions which is consistent with calcite re-precipitation taking place very close to equilibrium following the initial rapid saturation of the fluids by calcite.

Spatial heterogeneity of sediments on a topographically diverse descending plate at convergent margins: An example from the outer slope of the Mariana Trench

Unraveling deep ocean sediment composition and its controlling mechanisms is crucial to understanding the interplay between the Earth's surface and the deep Earth. Convergent margins are generally featured by various seafloor landforms, but how submarine topography affects sediment accumulation processes remains poorly understood. Here we focus on submarine surface sediments from the outer slope of the Mariana Trench (covering water depths of 1218–6310 m) and combine microscopic observation, clay mineralogy, and elemental geochemistry to characterize sediment composition and to reveal physical, chemical, and biological sedimentary processes at topographically diverse convergent margins. Smear slide observations indicate that foraminifera shells dominate in shallow areas, while pelagic clays and diatom oozes prevail in trench and slope areas. Clay minerals primarily consist of illite (26–98 %) with low crystallinity index values and diverse chemical index values, followed by chlorite (0–45 %) and kaolinite (0–29 %). Smectite (0–61 %, 46 of 70 samples have smectite contents <5 %) of the samples from the outer slope area is low but appears dominant close to the trench. Bulk element compositions are mainly affected by the relative calcareous, siliceous, and lithogenous components that dissolved or accumulated inconsistently as submarine topographic feature changes. Our results imply that submarine topography and sediment provenance lead to significant heterogeneity of sediment composition at topographically diverse convergent margins. The submarine topographic relief, together with associated bathymetry and deep-sea circulation primarily controls carbonate deposition on the Caroline Plateau, and influences the sinking particles (including diatom shells and arc volcanic-sourced materials) accumulation in the trench basin. Continent-derived aeolian dust introduces illite-rich detritus, while localized volcanism potentially contributes to smectite-rich sediment in certain topographic provinces. We propose that the deep sea sedimentation processes in the Mariana convergent system are more diverse and complex than those at the margins with relatively plain topographic characteristics (e.g., the Japan Trench). The significant heterogeneity on descending plates along convergent margins may lead to various subduction behaviors. These findings contribute to a better understanding of deep ocean sediment source-to-sink systems and have implications for Earth's material cycle and multi-sphere interaction studies.

A baroduric immobilized composite material promoting remediation of oil-polluted sediment at typical deep-sea condition: The performances and potential mechanisms

Contriving immobilized bioreagent is of great significance to enhance bioremediation of marine oil pollution. However, there remains a notable scarcity of correlational study conducted at deep sea condition. Herein, we first developed a baroduric microsphere encasing biotic and chemical materials to remediate oil-contaminated sediments at deep-sea microcosm. Total oil degradation efficiency of microsphere-treated group reached 71% within a month, representing an approximate 35% increase compared to natural remediation. Absorption and biodegradation by microsphere provided a comparable contribution to oil elimination. Together with scanning electron microscope observation, the physical mechanism was that the reticulate structure of microsphere surface facilitating oil adsorption and bacteria attachment. Via metabarcoding analysis for meta and metabolically-active microbes, we demonstrated the primary working center was located at the microsphere. Proteobacteria, Firmicutes, Bacteroidota and Desulfobacterota were the key activated bacteria. More importantly, we revealed the ecological mechanisms were associated with the following aspects: 1) the addition of microsphere significantly improved the metabolic activity of bacteria (particularly including several oil-degrading taxa); 2) the microspheres enhanced ecological stability and microbial functional diversification during bioremediation; 3) expressing activity of pathways involving oil component degradation, biosurfactant production, biofilm architecture, biogeochemical and energy cycling all were observed to be up-regulated in microsphere-treated samples. Altogether, our results provide important theoretical guidance and data support on application of immobilization technology in removing in-situ oil pollution of deep-sea sediment.

Ichnotaxonomy of microboring traces in marine aphotic depths

Microboring traces in carbonate skeletal fragments deposited in aphotic depths of the oceans are studied, evaluated, and described with respect to their marine ecology and palaeoecology as well as ichnotaxonomy. Sand-size deep sea sediment particles dredged from depths ranging between 600 and 3266 m of the Bermuda Pedestal, Central Atlantic Ocean, the Florida Escarpment, the Mediterranean Sea, the Red Sea and the Indian Ocean were studied. Following ichnological rules, trace fossils are described as ichnogenera and ichnospecies, defined as products of organismal behaviour. This, in our view, refers to the growth habit of microboring organisms in response to environmental stimuli within the substrate they penetrate. The problem of palaeobathymetry is discussed in conjunction with the distinction between light-dependent and light independent microboring organisms, with the emphasis on the latter. We considered this distinction to be important because only the light-dependent microborers have been recognized as indicators of ancient depositional depths, whereas the light-independent ones are expected to occur at any depth, subject to the availability of organic nutrients. Microboring organisms often leave morphologically similar traces due to convergent evolution. Their responses may change during their life cycle; they may produce different traces when pursuing their vegetative vs. reproductive functions. New ichnotaxa are described. All are regarded as organotrophs given their aphotic zone deep sea origin. This work presents the most complete set of deep sea microbial euendolith traces, to date.

Metal regeneration during an ex-situ disturbance experiment on deep-sea sediments from the polymetallic nodule area of western Pacific

Metal regeneration during an ex-situ disturbance experiment on deep-sea sediments from the polymetallic nodule area of western Pacific

Optimization of high-precision Bekker test plate structure for rarefied soils–example of deep-sea subsoils

The Bekker apparatus is an important tool for measuring soil bearing capacity; however, it is not entirely suitable for deep-sea sediments, and the size of the loading plate can affect the accuracy of the Bekker bearing parameters. This paper employs the Coupled Eulerian-Lagrangian (CEL) method to explore how different conditions impact Bekker bearing parameters. As the size of the loading plate gradually increases, the soil compression mechanism and load path reach a relatively stable state, leading to the stabilization of Bekker bearing parameters. The study proposes a range of plate sizes for deep-sea soils with varying strengths, noting that the physical mechanisms of low-strength soils are relatively simple, making them more consistent with the ideal conditions described by Bekker’s theory. Furthermore, an analysis of the relationship between Bekker bearing parameters and penetration depth reveals that the parameters corresponding to different strength soils vary almost parallel with depth. This is attributed to the same settlement path, where the distribution of loads and the geometrical shapes of the supporting structures remain unchanged, resulting in a similar trend in the variation of Bekker parameters. The research findings provide a theoretical basis for analyzing the bearing capacity characteristics of soft deep-sea soils.

Thaumarchaeota from deep-sea methane seeps provide novel insights into their evolutionary history and ecological implications

BackgroundAmmonia-oxidizing archaea (AOA) of the phylum Thaumarchaeota mediate the rate-limiting step of nitrification and remove the ammonia that inhibits the aerobic metabolism of methanotrophs. However, the AOA that inhabit deep-sea methane-seep surface sediments (DMS) are rarely studied. Here, we used global DMS metagenomics and metagenome-assembled genomes (MAGs) to investigate the metabolic activity, evolutionary history, and ecological contributions of AOA. Expression of AOA-specific ammonia-oxidizing gene (amoA) was examined in the sediments collected from the South China Sea (SCS) to identify their active ammonia metabolism in the DMS.ResultsOur analysis indicated that AOA contribute > 75% to the composition of ammonia-utilization genes within the surface layers (above 30 cm) of global DMS. The AOA-specific ammonia-oxidizing gene was actively expressed in the DMS collected from the SCS. Phylogenomic analysis of medium-/high-quality MAGs from 18 DMS-AOA indicated that they evolved from ancestors in the barren deep-sea sediment and then expanded from the DMS to shallow water forming an amoA-NP-gamma clade-affiliated lineage. Molecular dating suggests that the DMS-AOA origination coincided with the Neoproterozoic oxidation event (NOE), which occurred ~ 800 million years ago (mya), and their expansion to shallow water coincided with the Sturtian glaciation (~ 713 mya). Comparative genomic analysis suggests that DMS-AOA exhibit higher requirement of carbon source for protein synthesis with enhanced genomic capability for osmotic regulation, motility, chemotaxis, and utilization of exogenous organic compounds, suggesting it could be more heterotrophic compared with other lineages.ConclusionOur findings provide new insights into the evolutionary history of AOA within the Thaumarchaeota, highlighting their critical roles in nitrogen cycling in the global DMS ecosystems.EWMb7UH3jhRcWd8tHNUGyqVideo

Occurrence characteristics of authigenic pyrite in the deep-sea environment and its paleoceanographic implications based on core sediments from IODP Expedition 342 Site U1406

Although authigenic pyrite (FeS2) in marine sediment is an important proxy for oxygen conditions and microbial activities at its formation site, very little is known about its morphological characteristics in the deep-sea (> 3500 m) environment and their paleoceanographic implications. Here, we report the occurrence characteristics of authigenic pyrite including texture, size distribution, and relative content in North Atlantic deep-sea sediments from Integrated Ocean Drilling Program (IODP) Expedition 342 Site U1406. The results of electron microscopy show octahedral pyrite microcrystals of 1.5–4.0 μm, the spherical texture of greigite (Fe3S4), and large pyrite framboids (average, 25 μm) relative to those of the continental margin. These features may demonstrate an unchanged supersaturation level of the pore water for octahedral pyrite after its initial formation, replacement of the precursor greigite by pyrite, and an expanded pyrite framboid formation zone with a low sulfate reduction rate in deep-sea sediments, respectively. Although the size distribution of pyrite framboids measured in this study falls into the range of pyrites formed in the sulfate–methane transition zone, based on onboard geochemical data and previous research, these pyrites most likely formed through organoclastic sulfate reduction during diagenesis. We assessed the implications of these findings for paleoceanographic changes, as the formation of diagenetic pyrite is controlled by the supply rate and reactivity of organic matter, which are closely related to ocean productivity and circulation. A comparison between the profiles of relative pyrite content and benthic foraminiferal oxygen isotope (δ18Obf) values indicated that these factors were anti-phased during the late Oligocene. Based on our results, we conclude that the formation of diagenetic pyrite was favored during interglacial periods due to increases in the amount and reactivity of organic matter driven by more energetic formation of proto-North Atlantic Deep Water. Our findings provide new insights into the formation of authigenic pyrite in deep-sea environments and its potential as a paleoceanographic proxy.

Hydrocarbon-degrading microbial populations in permanently cold deep-sea sediments in the NW Atlantic

Permanently cold deep-sea sediments (2500–3500 m water depth) with and without indications of thermogenic hydrocarbon seepage were exposed to naphtha to examine the presence and potential of cold-adapted aerobic hydrocarbon-degrading microbial populations. Monitoring these microcosms for volatile hydrocarbons by GC–MS revealed sediments without in situ hydrocarbons responded more rapidly to naphtha amendment than hydrocarbon seep sediments overall, but seep sediments removed aromatic hydrocarbons benzene, toluene, ethylbenzene and xylene (BTEX) more readily. Naphtha-driven aerobic respiration was more evident in surface sediment (0–20 cmbsf) than deeper anoxic layers (>130 cmbsf) that responded less rapidly. In all cases, enrichment of Gammaproteobacteria included lineages of Oleispira, Pseudomonas, and Alteromonas known to be associated with marine oil spills. On the other hand, taxa known to be prevalent in situ and diagnostic for thermogenic hydrocarbon seepage in deep sea sediment, did not respond to naphtha amendment. This suggests a limited role for these prevalent seep-associated populations in the context of aerobic hydrocarbon biodegradation.

Comparative metagenomics highlights the habitat-related diversity in taxonomic composition and metabolic potential of deep-sea sediment microbiota

Comparative metagenomics highlights the habitat-related diversity in taxonomic composition and metabolic potential of deep-sea sediment microbiota

Fungi of the Arctic Seas

The abundance and diversity of mycelial fungi in the bottom sediments of the Arctic Ocean seas (the Greenland, Barents and Kara seas) were studied. Samples of the surface bottom sediments were collected during the 84th (July‒August 2021) and 86th (October‒November 2021) cruises of RV Akademik Mstislav Keldysh. The taxonomic affiliation of the isolated fungi was determined using polyphasic taxonomy. The isolated fungi belonged to 16 genera of different classes of ascomycetous, basidiomycetous, and zygomycetous fungi. The effect of cultivation temperature and different NaCl concentrations on fungal growth was determined, as well as the effect of cultivation conditions on the fatty acid profile for the strains capable of growth on media with increased osmotic potential. While fatty acid composition was shown to be affected by changes in environmental conditions, the response to osmotic stress differed among the studied cultures from deep-sea sediments.

Biotransformations of arsenic in marine sediments across marginal slope to hadal zone

Arsenic compounds are accumulating in deep ocean, but their ecological impacts on deep-sea ecosystem remain elusive. We studied 32 sediment cores (101 layers for metagenomes, along with 41 global reference sediment metagenomes) collected from the South China Sea and the Mariana Trench (MT), characterized with high arsenic accumulation in MT. In these metagenomes we revealed a significantly positive correlation between relative abundance of arsenite methyltransferase gene (arsM) and sampling depth, which suggests that arsenic methylation is the most prevalent arsenic biotransformation process in the deep sea. Lower relative abundance of arsenic efflux gene, compared with arsM, indicates that microbes in deep-sea sediments were prone to methylate arsenite and retain it rather than efflux it. Phylogenetic analysis identified seven clades of ArsM proteins, including two new clades derived primarily from deep-sea microorganisms. Five metagenome-assembled genomes containing aioA for arsenite oxidation also harbor carbon fixation genes in the deep-sea sediment layers, suggesting previously unnoticed contribution of arsenite-oxidizing autotrophic bacteria to the carbon cycle. Therefore, deep-sea microorganisms adopt different detoxification and transformation strategies in response to arsenic compounds, which renews our understanding of arsenic in their ecological impacts and potential contribution in deep ocean.

Response of sedimentary microbial community and antibiotic resistance genes to aged Micro(Nano)plastics exposure under high hydrostatic pressure

Several studies reported that the presence of microplastics (MPs)/nanoplastics (NPs) in marine environments can alter microbial community and function. Yet, the impact of aged MPs/NPs on deep sea sedimentary ecosystems under high hydrostatic pressure remains insufficiently explored. Herein, the sedimentary microbial community composition, co-occurrence network, assembly, and transfer of antibiotic resistance genes (ARGs) in response to aged MPs/NPs were investigated. Compared with the control, NPs addition significantly reduced bacterial alpha diversity (p < 0.05), whereas MPs showed no significant impact (p > 0.05). Moreover, networks under NPs exhibited decreased complexity than that under MPs and the control, including edges, average degree, and the number of keystone. The assembly of the microbial community was primarily governed by stochastic processes, and aged MPs/NPs increased the importance of stochastic processes. Moreover, exposure to MPs/NPs for one month decreased the abundance of antibiotic resistance genes (ARGs) (from 94.8 to 36.2 TPM), while exposure for four months increased the abundance (from 40.6 to 88.1 TPM), and the shift of ARGs in sediment was driven by both functional modules and microbial community. This study is crucial for understanding the stress imposed by aged MPs/NPs on sedimentary ecosystems under high hydrostatic pressure.

UPPER CRETACEOUS–LOWER EOCENE MUDSTONES OF THE OUTER CARPATHIANS (PETROCHEMICAL AND PALEOGEODYNAMIC ASPECTS)

The mineral types of the Upper Cretaceous–Lower Eocene greenish-gray mudstones of the Skyba and Boryslav-Pokuttya nappes of the Outer Carpathians were studied. According to sedimentological features, these mudstones belong to hemipelagites and contain information about the background sedimentation conditions of the deep-sea oceanic basin. The purpose of the work is to clarify the paleogeodynamic features of the basin based on the results of studying the material composition of the Upper Cretaceous–Lower Eocene background mudstones, which in particular are part of the variegated-colored horizons. Research methods were geological mapping, X-ray structural, sedimentological, lithostratigraphic, petrogeochemical, geodynamic analysis. On the classification module diagram (Na2O+K2O)/Al2O3–(FeO*+MnO+MgO)/SiO2, the figurative points of the contents of the Upper Cretaceous–Lower Eocene mudstones of the Stryi, Yamna, and Manyava suites fall into the fields corresponding to two mineral types: a mixture with a predominance of montmorillonite and three-component mixture of chlorite+montmorillonite+hydromica composition. According to the obtained data, a gradual change in the conditions of the sedimentary environment from the Late Cretaceous to the Eocene can be traced, with a successive increase in the normalized alkalinity modulus and a decrease in the phemic modulus. On the paleogeodynamic discriminant diagrams (FeO*+MgO)–TiO2 and F1–F2, figurative points of Upper Cretaceous–Lower Eocene mudstones form petrochemical distribution trends that cover the fields of geodynamic conditions from passive to active margins of the sedimentation basin. The Cretaceous–Paleogene pelagic mudstones of the Sicilian domain of the Alpine Belt have a similar distribution of petrochemical components. The mineral assemblages found in the mudstones of the Skyba and Boryslav-Pokuttya nappes are typical for deep-sea clay sediments of modern oceans. The presence of montmorillonite and chlorite in the background argillites indicates a high probability of a contribution to the background petrofund of the sedimentatary basin by a femic magmatic component of endogenous origin, realized as a manifestation of volcanic and hydrothermal activity, synchronous to sedimentogenesis. An exogenous source could be the magmatic material transferred or changed by the processes of halmyrolysis, which is not synchronous with sedimentation. The Late Cretaceous–Early Eocene geodynamic events of the Outer Carpathian sedimentary basin developed in front of the ALCAPA and Tisza-Dacia terranes of the Alpine Tethys. Convergence, which caused the formation of the accretionary prism in front of the terranes, could contribute to the riftogenic (?) opening, deepening of the Outer Carpathian Basin, where the penetration of synsedimentary endogenous material, mainly of the basic composition, took place. This material could be the rock-forming source of the basin substrate, on which the mineral assemblages of the background hemipelagic mudstones were formed.

Albusamides A-G: Hydroxylated Fatty Amine Derivatives from the Deep-Sea-Derived Actinomycete Streptomyces albus 228DD-066 and Their Cytotoxic Activity.

A series of new hydroxylated fatty amine derivatives, albusamides A-G (1-7), along with four known compounds (8-11), which are reported for the first time from a natural source, were isolated from the culture broth of Streptomyces albus 228DD-066 derived from a deep-sea sediment sample gathered off the coast of Dokdo Island, Republic of Korea. Their structures were elucidated through the comprehensive analysis of 1D and 2D NMR spectra and HRESIMS, and absolute configurations were determined using the modified Mosher's method. Biological evaluations against solid and blood cancer cell lines revealed that these new metabolites have moderate to strong cytotoxic activity. Compound 3 exhibited high cytotoxic activity with GI50 values ranging from 0.4 to 0.6 μM against solid cancer cell lines and exhibited the strongest cytotoxicity (GI50 value = 0.2 μM) against the WSU-DLCL2 blood cancer cell line.

Distribution Characteristics of Nitrogen-Cycling Microorganisms in Deep-Sea Surface Sediments of Western South China Sea.

Nitrogen-cycling processes in the deep sea remain understudied. This study investigates the distribution of nitrogen-cycling microbial communities in the deep-sea surface sediments of the western South China Sea, using metagenomic sequencing and real-time fluorescent quantitative PCR techniques to analyze their composition and abundance, and the effects of 11 environmental parameters, including NH4+-N, NO3--N, NO2--N, PO43--P, total nitrogen (TN), total organic carbon (TOC), C/N ratio, pH, electrical conductivity (EC), SO42-, and Cl-. The phylum- and species-level microbial community compositions show that five sites can be grouped as a major cluster, with sites S1 and S9 forming a sub-cluster, and sites S13, S19, and S26 forming the other; whereas sites S3 and S5 constitute a separate cluster. This is also evident for nitrogen-cycling functional genes, where their abundance is influenced by distinct environmental conditions, including water depths (shallower at sites S1 and S9 against deeper at sites S13, S19, and S26) and unique geological features (sites S3 and S5), whereas the vertical distribution of nitrogen-cycling gene abundance generally shows a decreasing trend against sediment depth. Redundancy analysis (RDA) exploring the correlation between the 11 environmental parameters and microbial communities revealed that the NO2--N, C/N ratio, and TN significantly affect microbial community composition (p < 0.05). This study assesses the survival strategies of microorganisms within deep-sea surface sediments and their role in the marine nitrogen cycle.

Diving into the Depths: Uncovering Microplastics in Norwegian Coastal Sediment Cores.

High concentrations of microplastics (MPs) have been documented in the deep-sea surface sediments of the Arctic Ocean. However, studies investigating their high-resolution vertical distribution in sediments from the European waters to the Arctic remain limited. This study examines MPs in five sediment cores from the Norwegian Coastal Current (NCC), encompassing the water-sediment interface and sediment layers up to 19 cm depth. Advanced analytical methods for MP identification down to 11 μm in size were combined with radiometric dating and lithology observations. MPs were present across all sediment cores, including layers predating the introduction of plastics, with concentrations exhibiting significant variation (54-12,491 MP kg-1). The smallest size class (11 μm) predominated in most sediment layers (34-100%). A total of 18 different polymer types were identified across all sediment layers, with polymer diversity and depth correlations varying widely between stations. Our findings suggest that differences in seafloor topography and the impact of anthropogenic activities (e.g., fishing) lead to varying environmental conditions at the sampling sites, influencing the vertical distribution of MPs. This challenges the reliability of using environmental parameters to predict MP accumulation zones and questions the use of MPs in sediment cores as indicators of the Anthropocene.