North Atlantic Deep Research Articles

Revisiting the relationship between the pore water carbon isotope gradient and bottom water oxygen concentrations

Reconstructing the oxygen content at the ocean floor provides insight into ocean circulation, ventilation, and carbon storage in the deep sea. The microbial breakdown of organic carbon within marine sediment through aerobic respiration consumes oxygen in the pore fluid and releases dissolved inorganic carbon. The offset in the carbon isotopic composition, δ13C, of epifaunal and infaunal foraminifera, Δδ13C, is considered to reflect the aerobic respiration of organic carbon and can be used to reconstruct the oxygen content of the bottom water. Previous work provided an empirical calibration that was suggested to be valid for oxygen reconstructions between 55 and 235 μmol kg−1. In this study, we apply a biogeochemical reactive transport model (RTM) to extend and update this calibration, allowing for the reconstruction of oxygen concentrations ([O2]) up to ∼300 μmol kg−1. Using the RTM and new bottom water [O2] and pore fluid measurements from Iberian Margin sediment cores, we also demonstrate that the calibration between the Δδ13C and bottom water [O2] must account for the coupled changes in the carbon system due to the respiration of organic carbon in the overlying ocean including the concentration and carbon isotopic composition of the dissolved inorganic carbon, and the δ13C of the organic carbon within the sediment column. We apply the improved calibration to reconstruct the changes in oxygen content at International Ocean Discovery Program (IODP) Site U1385 in the deep North Atlantic over the past 1.5 Myr.

Surface climate signals transmitted rapidly to deep North Atlantic throughout last millennium.

Instrumental observations of subsurface ocean warming imply that ocean heat uptake has slowed 20th-century surface warming. We present high-resolution records from subpolar North Atlantic sediments that are consistent with instrumental observations of surface and deep warming/freshening and in addition reconstruct the surface-deep relation of the last 1200 years. Sites from ~1300 meters and deeper suggest an ~0.5 degrees celsius cooling across the Medieval Climate Anomaly to Little Ice Age transition that began ~1350 ± 50 common era (CE), whereas surface records suggest asynchronous cooling onset spanning ~600 years. These data suggest that ocean circulation integrates surface variability that is transmitted rapidly to depth by the Atlantic Meridional Ocean Circulation, implying that the ocean moderated Earth's surface temperature throughout the last millennium as it does today.

Limited exchange between the deep Pacific and Atlantic oceans during the warm mid-Pliocene and Marine Isotope Stage M2 “glaciation”

Abstract. The Piacenzian stage (3.6–2.6 Ma) of the Pliocene is the most recent period where Earth experienced sustained intervals of global warmth analogous to predicted near-future climates. Despite considerable efforts to characterize and understand the climate dynamics of the Piacenzian, the deep ocean and its response to this warming remain poorly understood. Here we present new mid-Piacenzian Mg/Ca and Δ47 (“clumped isotope”) temperatures from the deep Pacific and North Atlantic oceans. These records cover the transition from Marine Isotope Stage (MIS) M2 – considered the most pronounced “glacial” stage of the Pliocene prior to the intensification of Northern Hemisphere glaciation – to the warm KM5 interglacial. We find that a large (> 4 ∘C) temperature gradient existed between these two basins throughout that interval, with the deep North Atlantic considerably warmer and likely saltier than at present. We interpret our results to indicate that the deep Pacific and North Atlantic oceans were bathed by water masses with very different physical properties during the mid-Piacenzian, and that only a limited deep oceanic exchange occurred between the two basins. Our results point to a fundamentally different mode of ocean circulation or mixing compared to the present, where heat and salt are distributed from the North Atlantic into the Pacific. The amplitude of cooling observed at both sites during MIS M2 suggests that changes in benthic δ18O associated with this cold stage were mostly driven by temperature change in the deep ocean rather than by ice volume.

Instability of Atlantic Meridional Overturning Circulation: Observations, Modelling and Relevance to Present and Future

The Atlantic Meridional Overturning Circulation (AMOC) has changed dramatically during the glacial–interglacial cycle. One leading hypothesis for these abrupt changes is thermohaline instability. Here, I review recent progress towards understanding thermohaline instability in both observations and modelling. Proxy records available seem to favor thermohaline instability as the cause of the abrupt climate changes during the glacial–deglacial period because the deep North Atlantic water mass and AMOC seemed to have changed before the North Atlantic climate. However, most fully Coupled General Circulation Models (CGCMs) so far seem to exhibit monostable AMOC, because (1) these models have failed to simulate abrupt AMOC changes unless they are forced by an abrupt change of external forcing and, (2) these models have shown opposite freshwater convergence from the current observations. This potential model bias in the AMOC stability leaves the model projection of the future AMOC change uncertain.

Dissolved gases in the deep North Atlantic track ocean ventilation processes

Gas exchange between the atmosphere and ocean interior profoundly impacts global climate and biogeochemistry. However, our understanding of the relevant physical processes remains limited by a scarcity of direct observations. Dissolved noble gases in the deep ocean are powerful tracers of physical air-sea interaction due to their chemical and biological inertness, yet their isotope ratios have remained underexplored. Here, we present high-precision noble gas isotope and elemental ratios from the deep North Atlantic (~32°N, 64°W) to evaluate gas exchange parameterizations using an ocean circulation model. The unprecedented precision of these data reveal deep-ocean undersaturation of heavy noble gases and isotopes resulting from cooling-driven air-to-sea gas transport associated with deep convection in the northern high latitudes. Our data also imply an underappreciated and large role for bubble-mediated gas exchange in the global air-sea transfer of sparingly soluble gases, including O2, N2, and SF6. Using noble gases to validate the physical representation of air-sea gas exchange in a model also provides a unique opportunity to distinguish physical from biogeochemical signals. As a case study, we compare dissolved N2/Ar measurements in the deep North Atlantic to physics-only model predictions, revealing excess N2 from benthic denitrification in older deep waters (below 2.9 km). These data indicate that the rate of fixed N removal in the deep Northeastern Atlantic is at least three times higher than the global deep-ocean mean, suggesting tight coupling with organic carbon export and raising potential future implications for the marine N cycle.

Evidence From Dissolved O2 Isotopes in North Atlantic Deep Water for a Recent Climatic Shift

AbstractAnthropogenic‐induced variations of the Atlantic Meridional Overturning Circulation (AMOC) and the associated Deep‐Water Formation (DWF) are a major concern. Using measurements of triple oxygen isotopes in the deep North Atlantic, we present novel evidence for a dramatic decadal to centennial shift in ocean conditions at the source region of DWF. These measurements suggest a recent decrease in the percentage of photosynthetic O2 derived from the source regions of AMOC in the Nordic Seas compared to the Little Ice Age. 1‐D model simulations suggest that a reduction in photosynthetic O2 production can explain the observed decrease. Alternatively, it may indicate a substantial decrease in sea‐ice cover and thus increased air‐sea gas exchange, bringing the isotopic composition of O2 closer to equilibrium with the atmosphere. Our novel data can serve as a benchmark for climate models.

Nuclear Reprocessing Tracers Illuminate Flow Features and Connectivity Between the Arctic and Subpolar North Atlantic Oceans.

Releases of anthropogenic radionuclides from European nuclear fuel reprocessing plants enter the surface circulation of the high-latitude North Atlantic and are transported northward into the Arctic Ocean and southward from the Nordic Seas into the deep North Atlantic, thereby providing tracers of water circulation, mixing, ventilation, and deep-water formation. Early tracer studies focused on 137Cs, which revealed some of the first significant insights into the Arctic Ocean circulation, while more recent work has benefited from advances in accelerator mass spectrometry to enable the measurement of the conservative, long-lived radionuclide tracers 129I and 236U. The latest studies of these tracers, supported by simulations using the North Atlantic-Arctic Ocean-Sea Ice Model (NAOSIM) and enhanced by the use of transit time distributions to more precisely accommodate mixing, have provided a rich inventory of transport data for circulation in the Arctic and North Atlantic Oceans that are of great importance to global thermohaline circulation and climate.

Changes in North Atlantic deep-water oxygenation across the Middle Pleistocene Transition.

The oxygen concentrations of oceanic deep-water and atmospheric carbon dioxide (pCO2) are intrinsically linked through organic carbon remineralization and storage as dissolved inorganic carbon in the deep sea. We present a high-resolution reconstruction of relative changes in oxygen concentration in the deep North Atlantic for the past 1.5 million years using the carbon isotope gradient between epifaunal and infaunal benthic foraminifera species as a proxy for paleo-oxygen. We report a significant (>40 micromole per kilogram) reduction in glacial Atlantic deep-water oxygenation at ~960 thousand to 900 thousand years ago that coincided with increased continental ice volume and a major change in ocean thermohaline circulation. Paleo-oxygen results support a scenario of decreasing deep-water oxygen concentrations, increased respired carbon storage, and a reduction in glacial pCO2 across the Middle Pleistocene Transition.

Cafeteria in extreme environments: Investigations on C. burkhardae and three new species from the Atacama Desert and the deep ocean

The heterotrophic nanoflagellate genus Cafeteria has been found to be ubiquitously distributed in the marine realm. We could isolate and cultivate ten strains morphologically similar to Cafeteria from various types of environment, including the deep sea, brackish waters and also meso- to hypersaline inland waters. Molecular analyses (18S rDNA, 28S rDNA) of newly isolated strains from the marine realm resulted in four more Cafeteria burkhardae strains from the deep North Atlantic Ocean and one new species (C. baltica sp. nov.) isolated from brackish waters of the Baltic Sea. Two strains isolated from the Atacama Desert belong to two new species (C. atacamiensis sp. nov. and C. paulosalfera sp. nov.), one other strain could not yet be assigned. Morphological characterizations of these strains obtained by high resolution microscopy revealed only small differences to already described species. However, molecular analyses showed a clear separation of the different Cafeteria species. We exposed several strains to different salt concentrations (2–150 PSU) to investigate their salinity tolerance. Only the marine strains of C. burkhardae were able to survive at salinities up to 150 PSU, indicating the possibility to inhabit a broader spectrum of habitats including hypersaline environments besides the deep sea with its high hydrostatic pressure.

Phylogeography of Paramuricea: The Role of Depth and Water Mass in the Evolution and Distribution of Deep-Sea Corals

The processes that control diversification and speciation in deep-sea species are poorly known. Here, we analyzed data produced by Restriction-Site Associated DNA Sequencing (RAD-Seq) of octocorals in the genus Paramuricea to elucidate diversification patterns and examine the role of environmental gradients in their evolution. The genus Paramuricea evolved around 8 MYA, with a high probability of a broad ancestral depth range from mesophotic depths to the deep sea. At around 1-2 MYA, the genus diversified across the continental slope of the deep North Atlantic, supporting the depth-differentiation hypothesis, with no invasions back into shallower depths (< 200 m). Diversification in the deep sea generally occurred from shallower, warmer waters to deeper, colder depths of the lower continental slope. We also found that the vertical structure of water masses was influential in shaping phylogeographic patterns across the North Atlantic Ocean, with clades found in either upper/intermediate or intermediate/deep water masses. Our data suggest that species diverged first because of environmental conditions, including depth, temperature, and/or water mass, and then diversified into different geographical regions multiple times. Our results highlight the role of the environment in driving the evolution and distribution of Paramuricea throughout the deep sea. Furthermore, our study supports prior work showing the utility of genomic approaches over the conventionally-used DNA barcodes in octocoral species delimitation.

Environmental Change at Deep-Sea Sponge Habitats Over the Last Half Century: A Model Hindcast Study for the Age of Anthropogenic Climate Change

Deep-sea sponges inhabit multiple areas of the deep North Atlantic at depths below 250 m. Living in the deep ocean, where environmental properties below the permanent thermocline generally change slowly, they may not easily acclimatize to abrupt changes in the environment. Until now consistent monitoring timeseries of the environment at deep sea sponge habitats are missing. Therefore, long-term simulation with coupled bio-physical models can shed light on the changes in environmental conditions sponges are exposed to. To investigate the variability of North Atlantic sponge habitats for the past half century, the deep-sea conditions have been simulated with a 67-year model hindcast from 1948 to 2014. The hindcast was generated using the ocean general circulation model HYCOM, coupled to the biogeochemical model ECOSMO. The model was validated at known sponge habitats with available observations of hydrography and nutrients from the deep ocean to evaluate the biases, errors, and drift in the model. Knowing the biases and uncertainties we proceed to study the longer-term (monthly to multi-decadal) environmental variability at selected sponge habitats in the North Atlantic and Arctic Ocean. On these timescales, these deep sponge habitats generally exhibit small variability in the water-mass properties. Three of the sponge habitats, the Flemish Cap, East Greenland Shelf and North Norwegian Shelf, had fluctuations of temperature and salinity in 4–6 year periods that indicate the dominance of different water masses during these periods. The fourth sponge habitat, the Reykjanes Ridge, showed a gradual warming of about 0.4°C over the simulation period. The flux of organic matter to the sea floor had a large interannual variability, that, compared to the 67-year mean, was larger than the variability of primary production in the surface waters. Lateral circulation is therefore likely an important control mechanism for the influx of organic material to the sponge habitats. Simulated oxygen varies interannually by less than 1.5 ml/l and none of the sponge habitats studied had oxygen concentrations below hypoxic levels. The present study establishes a baseline for the recent past deep conditions that future changes in deep sea conditions from observations and climate models can be evaluated against.

Rapid Communication of Upper‐Ocean Salinity Anomaly to Deep Waters of the Iceland Basin Indicates an AMOC Short‐Cut

AbstractThe mooring observations of the Overturning in the Subpolar North Atlantic Program reveal a significant freshening of the Iceland Scotland overflow waters that did not involve the Nordic Seas, the source of the dense Deep North Atlantic Water (Devana et al., 2021, https://doi.org/10.1029/2021GL094396). Their study suggests that this freshening at depth in the Iceland Basin stems from the largest upper‐ocean freshening event in 120 years that rapidly communicated through entrainment with the Iceland Scotland Overflow Waters. This communication, which is very likely driven by strong wintertime heat losses, strongly adds to our thinking that the progression of this extreme freshening event is providing us with a natural tracer that is helping to identify and understand key processes that determine the strength and variability of the overturning circulation and its sensitivity to ongoing climate change. Continued monitoring of the overturning in the North Atlantic is therefore necessary.

Adding pieces to the puzzle: insights into diversity and distribution patterns of Cumacea (Crustacea: Peracarida) from the deep North Atlantic to the Arctic Ocean.

The Nordic Seas have one of the highest water-mass diversities in the world, yet large knowledge gaps exist in biodiversity structure and biogeographical distribution patterns of the deep macrobenthic fauna. This study focuses on the marine bottom-dwelling peracarid crustacean taxon Cumacea from northern waters, using a combined approach of morphological and molecular techniques to present one of the first insights into genetic variability of this taxon. In total, 947 specimens were assigned to 77 morphologically differing species, representing all seven known families from the North Atlantic. A total of 131 specimens were studied genetically (16S rRNA) and divided into 53 putative species by species delimitation methods (GMYC and ABGD). In most cases, morphological and molecular-genetic delimitation was fully congruent, highlighting the overall success and high quality of both approaches. Differences were due to eight instances resulting in either ecologically driven morphological diversification of species or morphologically cryptic species, uncovering hidden diversity. An interspecific genetic distance of at least 8% was observed with a clear barcoding gap for molecular delimitation of cumacean species. Combining these findings with data from public databases and specimens collected during different international expeditions revealed a change in the composition of taxa from a Northern Atlantic-boreal to an Arctic community. The Greenland-Iceland-Scotland-Ridge (GIS-Ridge) acts as a geographical barrier and/or predominate water masses correspond well with cumacean taxa dominance. A closer investigation on species level revealed occurrences across multiple ecoregions or patchy distributions within defined ecoregions.

Sources and Transport Pathways of Precipitating Waters in Cold-Season Deep North Atlantic Cyclones

AbstractExtratropical cyclones are responsible for a large share of precipitation at midlatitudes and they profoundly impact the characteristics of the water cycle. In this study, we use the ERA5 and a cyclone tracking scheme combined with a Lagrangian diagnostic to identify the sources of moisture precipitating close to the center of 676 deep North Atlantic cyclones in winters 1979–2018. Moisture uptakes occur predominantly in originally cold and dry air heated over the North Atlantic, in particular, over the warm waters of the Gulf Stream, whereas more remote sources from land or the subtropics are less important. Analyzing the dynamical environment of moisture uptakes, we find that moisture precipitating during the cyclone intensification phase originates in the precyclone environment in the cold sectors of preceding cyclones and the cyclone–anticyclone interaction zone. These moisture uptakes are linked to the cyclone’s ascent regions via the so-called feeder airstream, a northeasterly cyclone-relative flow that arises due to the cyclone propagation exceeding the advection by the low-level background flow. During the decay phase, more and more of the moisture originates in the cyclone’s own cold sector. Consequently, the residence time of precipitating waters in cyclones is short (median of ≈2 days) and transport distances are typically less than the distance traveled by the cyclone itself. These findings emphasize the importance of preconditioning by surface fluxes in the precyclone environment for the formation of precipitation in cyclones and suggest an important role for the hand-over of moisture from one cyclone to the next within a storm track.

North Atlantic Deep Water during Pleistocene interglacials and glacials

The global ocean overturning circulation is a major means of distributing heat around the Earth, and an important trigger or amplifier of climate change. This study presents a 1.5-Myr-long neodymium (Nd) isotope record of Deep Sea Drilling Project Site 607, in the core of present-day North Atlantic Deep Water (NADW), the water mass that drives the overturning circulation and its Atlantic end-member (Broecker, 1991; Gordon, 1991), in order to document its composition through time. This time interval is marked by major changes in fundamental aspects of the Earth's climate, including the Mid-Pleistocene Transition (MPT) from ∼41-kyr to ∼100-kyr interglacial-glacial cycles and more intense glacials. The new record, mainly focusing on interglacial and glacial peaks, shows a pattern that mimics the record of benthic foraminiferal δ18O (Lisiecki and Raymo, 2005) in that the magnitude of interglacial-glacial Nd isotope shifts were smaller in the 41-kyr world than in the 100-kyr world. During the “900 ka event” (Clark et al., 2006), between ∼960 and 860 ka, marking the first 100-kyr interglacial-glacial cycle, the Nd isotope ratios shift abruptly to higher values, consistent with increased incursion of Southern Ocean water masses into the deep North Atlantic. This pattern was previously observed in the South Atlantic over the same time interval and interpreted as a weakened presence of NADW signal that reflected a period of disrupted deep ocean overturning circulation, termed the “MPT-AMOC crisis” (Pena and Goldstein, 2014). The Site 607 data support this interpretation and show the effects to be basin-wide. Following the disruption, an enhanced southern-sourced Nd isotope signature remained for ∼200 kyr during the period of “lukewarm interglacials” (Howe and Piotrowski, 2017; Jaccard et al., 2013), consistent with weaker overturning circulation. With the exception of this “MPT-AMOC crisis and recovery” interval, the Nd isotope ratios during interglacial peaks have been similar to present-day NADW, indicating similar interglacial North Atlantic ocean circulation dynamics both before and after the MPT. This contrasts with the pattern during glacial periods of the 100-kyr world, during which Nd isotopes have continued to follow the pattern of the 900-kyr event indicating a strong southern water mass signal, interpreted as intensified incursions of Southern Ocean water into the deep North Atlantic and consistent with a generally weaker overturning circulation during glacials.

Biogeography, diversity and environmental relationships of shelf and deep-sea benthic Amphipoda around Iceland.

The waters around Iceland, bounding the Northern North Atlantic and the Nordic seas, are a region characterized by complex hydrography and seabed topography. This and the presence of the Greenland-Iceland-Faroe-Scotland ridge (GIFR) are likely to have a major impact on the diversity and distribution of the benthic fauna there. Biodiversity in this region is also under increasing threat from climate-induced changes, ocean warming and acidification in particular, affecting the marine realm. The aim of the present study was to investigate the biodiversity and distributional patterns of amphipod crustaceans in Icelandic waters and how it relates to environmental variables and depth. A comprehensive data set from the literature and recent expeditions was compiled constituting distributional records for 355 amphipod species across a major depth gradient (18–3,700 m). Using a 1° hexagonal grid to map amphipod distributions and a set of environmental factors (depth, pH, phytobiomass, velocity, dissolved oxygen, dissolved iron, salinity and temperature) we could identify four distinct amphipod assemblages: A Deep-North, Deep-South, and a Coastal cluster as well as one restricted to the GIFR. In addition to depth, salinity and temperature were the main parameters that determined the distribution of amphipods. Diversity differed greatly between the depth clusters and was significantly higher in coastal and GIFR assemblages compared to the deep-sea clusters north and south of the GIFR. A variety of factors and processes are likely to be responsible for the perceived biodiversity patterns, which, however, appear to vary according to region and depth. Low diversity of amphipod communities in the Nordic basins can be interpreted as a reflection of the prevailing harsh environmental conditions in combination with a barrier effect of the GIFR. By contrast, low diversity of the deep North Atlantic assemblages might be linked to the variable nature of the oceanographic environment in the region over multiple spatio-temporal scales. Overall, our study highlights the importance of amphipods as a constituent part of Icelandic benthos. The strong responses of amphipod communities to certain water mass variables raise the question of whether and how their distribution will change due to climate alteration, which should be a focus of future studies.

Northern High-Latitude Organic Soils As a Vital Source of River-Borne Dissolved Iron to the Ocean.

Organic soils in the Arctic-boreal region produce small aquatic humic ligands (SAHLs), a category of naturally occurring complexing agents for iron. Every year, large amounts of SAHLs-loaded with iron mobilized in river basins-reach the oceans via river runoff. Recent studies have shown that a fraction of SAHLs belong to the group of strong iron-binding ligands in the ocean. That means, their Fe(III) complexes withstand dissociation even under the conditions of extremely high dilution in the open ocean. Fe(III)-loaded SAHLs are prone to UV-photoinduced ligand-to-metal charge-transfer which leads to disintegration of the complex and, as a consequence, to enhanced concentrations of bioavailable dissolved Fe(II) in sunlit upper water layers. On the other hand, in water depths below the penetration depth of UV, the Fe(III)-loaded SAHLs are fairly resistant to degradation which makes them ideally suited as long-lived molecular transport vehicles for river-derived iron in ocean currents. At locations where SAHLs are present in excess, they can bind to iron originating from various sources. For example, SAHLs were proposed to contribute substantially to the stabilization of hydrothermal iron in deep North Atlantic waters. Recent discoveries have shown that SAHLs, supplied by the Arctic Great Rivers, greatly improve dissolved iron concentrations in the Arctic Ocean and the North Atlantic Ocean. In these regions, SAHLs play a critical role in relieving iron limitation of phytoplankton, thereby supporting the oceanic sink for anthropogenic CO2. The present Critical Review describes the most recent findings and highlights future research directions.

Impact of Southern Ocean surface conditions on deep ocean circulation during the LGM: a model analysis

Abstract. Changes in water mass distribution are considered to be a significant contributor to the atmospheric CO2 concentration drop to around 186 ppm recorded during the Last Glacial Maximum (LGM). Yet simulating a glacial Atlantic Meridional Overturning Circulation (AMOC) in agreement with paleotracer data remains a challenge, with most models from previous Paleoclimate Modelling Intercomparison Project (PMIP) phases showing a tendency to simulate a strong and deep North Atlantic Deep Water (NADW) instead of the shoaling inferred from proxy records of water mass distribution. Conversely, the simulated Antarctic Bottom Water (AABW) is often reduced compared to its pre-industrial volume, and the Atlantic Ocean stratification is underestimated with respect to paleoproxy data. Inadequate representation of surface conditions, driving deep convection around Antarctica, may explain inaccurately simulated bottom water properties in the Southern Ocean. We investigate here the impact of a range of surface conditions in the Southern Ocean in the iLOVECLIM model using nine simulations obtained with different LGM boundary conditions associated with the ice sheet reconstruction (e.g., changes of elevation, bathymetry, and land–sea mask) and/or modeling choices related to sea-ice export, formation of salty brines, and freshwater input. Based on model–data comparison of sea-surface temperatures and sea ice, we find that only simulations with a cold Southern Ocean and a quite extensive sea-ice cover show an improved agreement with proxy records of sea ice, despite systematic model biases in the seasonal and regional patterns. We then show that the only simulation which does not display a much deeper NADW is obtained by parameterizing the sinking of brines along Antarctica, a modeling choice reducing the open-ocean convection in the Southern Ocean. These results highlight the importance of the representation of convection processes, which have a large impact on the water mass properties, while the choice of boundary conditions appears secondary for the model resolution and variables considered in this study.

Fungi populate deep-sea coral gardens as well as marine sediments in the Irish Atlantic Ocean.

Fungi populate deep Oceans in extreme habitats characterized by high hydrostatic pressure, low temperature and absence of sunlight. Marine fungi are potential major contributors to biogeochemical events, critical for marine communities and food web equilibrium under climate change conditions and a valuable source of novel extremozymes and small molecules. Despite their ecophysiological and biotechnological relevance, fungal deep-sea biodiversity has not yet been thoroughly characterized. In this study, we describe the culturable mycobiota associated with the deepest margin of the European Western Continental Shelf: sediments sampled at the Porcupine Bank and deep-water corals and sponges sampled in the Whittard Canyon. Eighty-seven strains were isolated, belonging to 43 taxa and mainly Ascomycota. Ten species and four genera were detected for the first time in the marine environment and a possible new species of Arachnomyces was isolated from sediments. The genera Cladosporium and Penicillium were the most frequent and detected on both substrates, followed by Candida and Emericellopsis. Our results showed two different fungal communities: sediment-associated taxa which were predominantly saprotrophic and animal-associated taxa which were predominantly symbiotic. This survey supports selective fungal biodiversity in the deep North Atlantic, encouraging further mycological studies on cold water coral gardens, often overexploited marine habitats.

Deep-ocean circulation in the North Atlantic during the Plio-Pleistocene intensification of Northern Hemisphere Glaciation (~2.65–2.4 Ma)

Through transporting heat around the world and thereby regulating global climate, ocean circulation is an integral part of Earth's climate system that likely changed substantially on glacial-interglacial timescales in the deep North Atlantic Ocean. However, quantitative records on deep-water dynamics in the North Atlantic older than ~1 Myr are sparse and typically of low temporal resolution. Here we provide a new record on northern- versus southern-sourced waters in the deep North Atlantic at a yet unprecedented temporal resolution. Our record is based on a novel approach (Atlantic-Pacific bottom-water temperature (BWT) difference) for an interval comprising the onset of larger-scale glaciations in the Northern Hemisphere, the Plio-Pleistocene transition (~2.65–2.4 Ma; Marine Isotope Stages G1–95). We thus have generated a new, millennial-scale-resolution BWT record based on benthic foraminiferal Mg/Ca from Ocean Drilling Program Site 849 in the East Pacific backed up with a new neodymium-isotope record on glacial-interglacial-scale resolution. The difference between our new BWT record (Site 849) and previously available BWTs from Integrated Ocean Drilling Program Site U1313 in the North Atlantic was used to decipher changes between southern- and northern-sourced waters in the deep North Atlantic. In accordance with previous studies, we document an increased influence of deep waters from high southern latitudes during glacials at the expense of northern-sourced waters. Perhaps even more importantly, our new record is highly relevant for the determination of geochemically-based sea-level records from the deep North Atlantic, which was yet limited by quantitative estimates of deep-water masses at a sufficient, (sub-)millennial-scale resolution.