A simple and efficient microwave-assisted extraction method using deep eutectic solvent was developed for the simultaneous analysis of four biogenic amines (putrescine, tyramine, cadaverine and histamine) in Doogh (yogurt-based beverage) samples. Key parameters like precipitating agent volume, vortexing time, microwave power and exposure time were optimized. Zinc sulfate was utilized for proteins precipitation prior to extraction of analytes with the deep eutectic solvent choline chloride/p-anisidine. The analytes were derivatized with dansyl chloride and analyzed by HPLC-DAD. The method showed good linearity (r2 ≥ 0.994) over wide ranges with low limits of detection (0.52–0.78 ng mL−1) and quantification (1.7–2.5 ng mL−1). Extraction recoveries ranged from 68–72 % and relative standard ≤8.1 %. Negligible matrix effects were observed. When the method was applied to real Doogh samples, tyramine, histamine, and putrescine were detected, while cadaverine was not found. The developed method provided reliable results in terms of sensitivity and selectivity.