Decreasing Linear Effect Research Articles

Can condensed tannins improve fermentation patterns and the nutritive value of sorghum silage?

Context For an ensiling to succeed, the knowledge about the factors that might influence the fermentation dynamics and nutrient loss is mandatory. One of the strategies is the use of additives such as tannins, as they can decrease proteolysis and increase the total organic acids. Aims The aim was to investigate the effects of tannins on fermentation patterns and nutritional value of sorghum silage. Methods Silages containing 0.0, 7.5, 15.0, 22.5, and 30.0 g/kg of tannin on fresh matter were studied. To produce the silage, the tannin was added to the forage and manually homogenised before ensiling. The study was conducted in three experiments. In the first experiment, five replications of each level of inclusion were produced and assigned in a completely randomised design. The chemical composition and fermentation patterns of all experimental units were evaluated. The second experiment consisted of analysing the aerobic deterioration by using a PET bottles system. This experiment was conducted in a completely randomised split plot, with five replications and five treatments with the inclusion of different levels of tannin (main plots) and the hours of exposure of silage to air (subplot). In the third experiment, five levels of tannin (main plots) and incubation times (subplots) were evaluated as for in situ digestibility (ruminal degradability) in a randomised blocks design with three replications (animals). Key results The inclusion of tannins in sorghum silage caused quadratic effects on pH, acetic acid, lactic acid, total organic acids, ammonia nitrogen, dry matter content, crude protein content and in situ digestibility, when the level varied from 10.5 to 22.7 g tannin/kg fresh matter. There was a decreasing linear effect for aerobic deterioration as increasing levels of tannin in sorghum silage. Conclusions The inclusion of tannin as an additive in sorghum silage is recommended for the production of good quality silage. The use of condensed tannins optimise fermentation patterns (especially increasing lactic acid and decreasing ammoniacal nitrogen), chemical composition, providing less aerobic deterioration and improve digestibility. Implications This study revealed that condensed tannins can improve fermentation patterns and the nutritive value of sorghum silage.

Pulsed electric field (PEF) processing of microalga Chlorella vulgaris and its digestibility in broiler feed

Microalgae have potentially beneficial effects on animal health and nutritional value when added to feed. Crucial hereby is that intracellular bio-active molecules are released in the intestinal tract. Digestibility of Chlorella vulgaris and its impact on total digestibility of broiler feed is a first step in assessing its characteristics as feed supplement. Different methods could be used to increase the digestibility of the algae. Among other, pulsed electric field (PEF) and freezing to disrupt autotrophic (A) and heterotrophic (H) Chlorella vulgaris cells was assessed to increase their availability followed by in-vivo trials. In these trials effect of algae type (A and H) and effect of PEF-processing was evaluated on the apparent nutrient digestibility. Pulsed electric field showed to have a disruption efficiency of 83.90% and 79.20% for heterotrophic and autotrophic C. vulgaris respectively. Freezing C. vulgaris only showed efficiencies ranging from 3.86 to 11.58%. In the in-vivo trials, microscopic counting of intact C. vulgaris cells showed an increase in digested intact C. vulgaris cells of PEF-processed C. vulgaris compared to nonprocessed cells ranging from 12.16% to 15.20%. Autotrophic C. vulgaris had a higher digestibility compared to heterotrophic C. vulgaris, with an increase of 7.29, 9.44, and 17.29% in digestibility of C. vulgaris in the 1, 2, and 5% feed respectively. Feeds with PEF-processed C. vulgaris showed no significant increase in digestibility compared to nonprocessed C. vulgaris supplemented feeds. The 5% C. vulgaris feeds showed lower fat digestibility than the 1 and 2% and control feeds. Protein digestibility was lower for all C. vulgaris feeds compared to the control feed. There was a significant linear decreasing effect (P < 0.001) for all digestibility parameters. Except for crude ash digestibility, which first lowered for the 1 and 2% feeds, but then increased at 5% inclusion. Considering this study, including low dosages of 1 and 2% of C. vulgaris in broiler feed does not compromise its digestibility.

Doses of orange (Citrus sinensis) essential oil for Nellore steers fed with a forage-based diet

The aim was to evaluate whether the doses of orange essential oil supplementation impact on dry matter intake (DMI), ruminal fermentation parameters, apparent nutrient digestibility, and methane production in Nellore steers fed with forage-based diet. A total of 32 rumen-cannulated Nellore steers (initial BW= 335±25 kg; age= 20±1 month) were assigned to individual pens in a randomized complete block design. Within block, steers were randomly assigned to treatments: CON= diet without orange essential oil (EO); 100EO= CON diet plus 100 mg orange EO/kg DM diet; 200EO= CON diet plus 200 mg orange EO/kg DM diet; and 500EO= CON diet plus 500 mg orange EO/kg DM diet. The main active compounds of the orange EO used were limonene (88.0 %) and myrcene (5.79 %). The experimental period lasted 28d, with 22d for adaptation and 6d for sample collection. From d23 to 27, samples of haylage, concentrate, and total fecal production were collected to determine the total tract apparent nutrient digestibility. Ruminal fluid was obtained from d28 at 0, 6, and 12 h after feeding to determine ruminal fermentation parameters and methane production. The difference was considered significant when P ≤ 0.05. No treatment effects were detected (P>0.05) to DMI or specific nutrient intake. The increasing levels of EO did not affect (P>0.05) the total apparent nutrient digestibility. No treatment effects were detected (P>0.05) for ruminal fermentation parameters in vivo. In ex situ, there was a treatment effect for acetate, total short-chain fatty acids (SCFA), methane, and relative energy loss (REL). For acetate, an increasing linear effect was observed for treatments that received EO for the variables production (g/kg) (P = 0.002) and gross energy (MJ/kg) (P = 0.002), with a higher concentration for the 500EO. For the total production of SCFA, there was an increasing linear effect of treatment with a higher concentration for 500EO for the variables production (g/kg) (P = 0.001) and gross energy (MJ/kg) (P = 0.001). For methane, a quadratic treatment effect was observed for the variables production (g/kg) (P = 0.045) and gross energy (MJ/kg) (P = 0.046), with lower concentrations for the 200EO. There was a decreasing linear treatment effect for REL(%) (P<0.001). The results for the use of orange EO as a manipulator of ruminal fermentation were promising and the dose of 500 mg/kg of DM showed a greater capacity to increase the energy efficiency of fermentation, with greater production of acids and lower production of methane, without affecting the voluntary intake of steers.

Physicochemical and microbiological parameters of rumen fluid of lactating goats fed diets containing crude glycerin from waste frying oil used in biodiesel production.

Crude glycerin from the biodiesel industry is a alternative energy source used to replace part of the energy components of the diet, since the nutritional value of glycerol makes it suitable for use in ruminant diets. The objective was to analyze the effects including crude glycerin (CG) derived from the production of biodiesel from residual frying oil, in diets of lactating goats on the physicochemical and microbiological parameters of rumen fluid. Eight 2-year-old goats weighting approximately 42.06 ± 3.5kg of body weight (BW) were kept in individual stalls, receiving diets containing 0, 7, 14 and 21% of CG (% DM). The experiment was carried out according to a double 4 × 4 Latin square and lasted for 80days. Orthogonal contrasts were tested to determine linear and quadratic effects, as well as the effects of 0% CG in the diet in comparison to the other treatments. There was a linear decreasing effect on DM intake (P = 0.001) and neutral detergent fiber (P = 0.0001), as well as a quadratic effect (P = 0.0002) on ether extract (EE) intake with increasing level of CG in the diet. Including CG affected motility and density of the protozoa (P = 0.0001). Treatments had no effect on pH, N-NH3 concentration and percentage of protozoa in the rumen liquid. There was increasing linear effect on sedimentation and flotation time (P = 0.001). Therefore, including 14 and 21% CG in the diet as a source of glycerol decreased nutrient intake and altered the ruminal microbiota, but pH and N-NH3 were not affected. Thus, to avoid impairment to the physicochemical and microbiological parameters of the rumen liquid of goats, the inclusion of CG should not exceed 7% DM.

Effects on meat quality of Yerba mate (Ilex paraguariensis) in the diet of chickens

The objective is to evaluate the effects of the inclusion of yerba mate in the diet on the yield and quality of chicken meat stored frozen for 120 days after slaughter. The birds were distributed in a completely randomized design, with four treatments, and eight replications with 25 birds each. The treatments consisted of providing a basal diet with 1, 2, 4, and 6 g kg-1 of ground dehydrated Yerba mate. At slaughter, temperature, pH, and carcass yield were measured. 120 days after meat freezing, measurements of pH, colorimetry, cooking weight loss (CWL), shear force, and water retention capacity were estimated. The parameters evaluated after slaughter showed regression effects on breast and drumstick temperature. Breast temperature showed a decreasing linear effect (R2=0.61). For the variable thigh temperature, there was a negative quadratic effect (R2=0.66). The quality characteristics of breast and drumstick evaluated 120 days after freezing showed an increasing linear effect of drumstick CWL (R2=0.38). Yerba mate can be included in up to 6 g kg-1 in chicken feed without changing pH, color, and meat tenderness after 120 days of freezing, maintaining carcass and cut yields.

Reductions in the water supply to crossbred Santa Inês ewes in the Brazilian semi-arid: Apparent nutrient digestibility, water and nitrogen balance, and performance

SummaryThis study aimed to evaluate the intake and nutrient digestibility, water, and nitrogen balance of Santa Inês crossbred ewes under confinement receiving reductions in the water supply. Thirty-two Santa Inês crossbred ewes, at an average body weight of 32.2 ± 7.4 kg and age of 2.3 ± 0.99 years, were distributed in a completely randomized design, with four treatments, and eight animals per treatment, the treatments were 100%, 80%, 60%, and 40% of ad libitum water. Nutrient intake and digestibility, water and nitrogen balance, and performance were evaluated. The reductions in the water supply did not promote significant alterations, in the nutrient intake, or apparent digestibility of the animals (P > 0.05). A linear decreasing effect was observed for free water intake (P < 0.001), total water intake (P < 0.001), total urine output (P = 0.008), water excreted via urine (P = 0.006), water excreted via feces (P = 0.006), total water losses (P < 0.001), water absorbed (P < 0.001), and nitrogen excreted via feces (P = 0.002). The water and nitrogen balance and performance were not influenced by water offers (P > 0.05). It was concluded that water offers of up to 40% of ad libitum intake, do not promote negative effects on intake and apparent nutrient digestibility, mineral intake, nitrogen balance, and water balance in Santa Inês ewes.

Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles

This study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control – 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (α-MEM+) or α-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in α-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicles. Moreover, secondary follicles from of ewes that receive 60% water intake increased follicular growth after in vitro culture with 25 ng/mL leptin.

Does macauba cake alter nutrient digestibility and microorganism population in the rumen of sheep?

Twenty Santa Inês male sheep were used in a randomized block design to evaluate the effects of different levels of macauba cake (MC) on nutrient digestibility and the population of microorganism in the rumen. The animals were divided into four groups according to MC levels (0, 10, 20, and 30% of DM) and initial body weight ranging from 32.75 to 52.17kg. Diets were isonitrogenous and formulated to attend metabolizable energy requirements, and feed intake was regulated with 10% allowance for leftovers. Each experimental period lasted 20days, with the final 5days reserved for sample collection. Macauba cake inclusion did not affect the dry matter, organic matter, or crude protein intake but increased the ether extract, neutral detergent fiber, and acid detergent fiber intake, mainly because of changes in the concentrations of these components in diets with a higher level of MC. With MC inclusion, a linear decreasing effect was observed for the dry matter and organic matter digestibility, and a quadratic effect with a maximum point of 21.5% was observed for the acid detergent fiber digestibility. A relative reduction of 73% in anaerobic fungal populations was observed with the lowest level of MC inclusion, and a relative increase of 162% in methanogenic populations was observed with the highest level of MC inclusion. The increasing level of macauba cake up to 30% of the diet of lambs reduced the dry matter digestibility and reduced anaerobic fungi but increased methanogenic population.

Influence of cactus pear cultivation density on the physicochemical characteristics of the soil

The objective was to evaluate the effect of the planting density of cactus pear cv. Mexican elephant ear under soil physical and chemical properties. Four planting densities were tested: 30,000, 45,000, 60,000 and 75,000 plants distributed in a randomised block design with four replications. After 360 days of cultivation, soil samples were taken in different profiles (0.0–0.10, 0.10–0.20, 0.20–0.30 and 0.30–0.40 m). The physical–chemical analysis included soil density, particle density, porosity, pH, electrical conductivity (EC), phosphorus (P), potassium (K+), sodium (Na+), calcium (Ca2+), magnesium (Mg2+), copper (Cu2+), iron (Fe2+), manganese (Mn2+), zinc (Zn2+), exchangeable acidity (H+Al), sums of bases (SB), cation exchange capacity and base saturation (V). The results were tested using analysis of variance, regression at 5% probability and principal component analysis (PCA). Physical properties along the soil profile and soil chemical properties in the 0–0.10 m profile were not altered (P > 0.05) by the density. There was a decreasing linear effect (P < 0.05) of density on the P content in the 0.10–0.20, 0.20–0.30 and 0.30–0.40 m profiles. Mg, Zn and V in the 0.10–0.20 m profile and Ca in the 0.30–0.40 m profile decreased linearly (P < 0.05) as a function of density. There was an increasing linear effect (P < 0.05) of planting density on Fe in the 0.20–0.30 m profile. The PCA showed that the factor loadings of the chemical properties of the soil in the different profiles were greater than 75% in the first two components. Except for the 0–0.10 m profile, with 55.77%. Minerals that contributed most to PC were Ca, K, P, Mg and Mn. In this sense, increasing the planting density of cactus pear without the use of fertiliser demands more from the soil, recommending lower densities between 30,000 and 45,000 plants ha−1.

Crambe cake protein replacing soybean meal protein and its effects on performance, carcass traits and meat quality of lambs

ABSTRACT The objective was to evaluate different levels of crambe cake (CC) protein (0, 250, 500, 750 and 1000 g/kg DM) as a replacement for soybean meal protein in diets for lambs and their effect on performance, carcass traits and meat quality. Forty not castrated crossbred male lambs, weighing 20 ± 3.45 kg, were settled in a completely randomized design with eight replicates per treatment. Final body weight (BW), dry matter intake per kg BW (DMI), DMI over metabolic weight and average daily gain showed linear decreasing effect (p < 0.05), meanwhile, feed efficiency presented a quadratic effect for replacement levels. There was a quadratic effect with replacement levels of CC protein for hot carcass weight; cold carcass weight; cooling loss index; muscle fat thickness; leg perimeter; leg depth and carcass finishing. Hot carcass yield, carcass length; shoulder clods weight, rib weight and the leg weight showed a linear decrease within BW. Bones and muscle weight of shoulder clods showed a decreasing linear effect. Replacing up to 500 g/kg DM of protein of soybean meal protein for CC protein does not affect performance, carcass traits and meat quality.

Performance, metabolism, and meat quality of broilers fed dry brewery residue.

A study was conducted to assess the chemical composition and apparent metabolizable energy (AME), nitrogen-corrected AME (AMEn), and their respective coefficients of dry brewery residue (DBR) for broilers. A second study was conducted to evaluate the increasing inclusions of DBR in broiler diets and its effects on growth performance, carcass traits, blood metabolites, and meat quality. In the first trial (metabolism assay), a total of 250 male broilers were assigned to 5 diets (0, 100, 200, 300, and 400g DBR/kg) composed by 5 replications with 10 animals per cage in a completely randomized design in the period from days 11 to 21. There was an increasing linear effect for AME, AMEn, metabolizable coefficient of gross energy (MCGE), and MCGE corrected for N (MCGEn). In the second experiment, a total of 840 one-day-old male broiler chickens were assigned to 6 treatments (0, 20, 40, 60, 80, and 100g DBR/kg) with 7 replications and 20 animals per pen, from days 0 to 42. Growth performance was determined at days 21 and 42. There was an increasing linear effect for weight gain in the period from days 0 to 21 and for feed intake from days 0 to 42. A linear decreasing effect was observed at day 21 for cholesterol and quadratic effect for uric acid (UA), aspartate aminotransferase, and albumin. At day 42, there was a quadratic effect for UA and creatinine. At day 21, an increasing linear effect was observed for protein deposition rate. In conclusion, the inclusion of DBR up to 100g/kg improved WG in the starter phase and did not negatively affect the productive parameters of broilers from days 0 to 42.

Meat quality in ewes submitted to reduction in water supply

The aim of this study was to evaluate the reduction in water supply (ad libitum intake - 100%, or 80%, 60% and 40% of ad libitum intake) on the proximate composition, physicochemical properties, mineral composition and fatty acid profile of ewe meat. Thirty-two crossbred Santa Inês ewe (n = 8 per treatment) were distributed in a randomized block design, receiving a diet consisting of elephant grass and concentrate (70:30). The experimental period lasted 63 days, preceded by 14 days of adaptation. Ewes were slaughtered at the end of experimental period, at a mean final body weight of 37.63 kg. Carcasses were cut lengthwise and the loin (longissimus lumborum) was taken of the left half carcass to evaluate the proximate composition, the physicochemical characteristics, the minerals content and fatty acid profile. The reduction in water supply resulted in a quadratic effect for resilience (p < 0.05), Lightness L* (p < 0.05), potassium (p < 0.05) and iron content (p < 0.001). There was a decreasing linear effect for magnesium content (p < 0.05), copper content (p < 0.001), C18:1n7t content (p < 0.05) and Σmonounsaturated content (p < 0.05). Crossbred Santa Inês ewe tolerate water restriction up to 40% voluntary intake without compromising the meat quality.

The inclusion of cactus pear changes the fermentation process, chemical composition and aerobic stability of arboreal cotton silages

The objective was to evaluate the fermentation process, nutritional quality, and aerobic stability of mixed silages of arboreal cotton and cactus pear. This was a completely randomized design, with five levels of inclusion of cactus pear (0, 15, 30, 45, and 60%) in the arboreal cotton silage and four replications per treatment, totaling 20 experimental units. Data were submitted to analysis of variance and regression at a 5% probability level for type I error. The inclusion of cactus pear resulted in increased losses from gases (P&lt;0.001), effluents (P&lt;0.001), buffering capacity (P&lt;0.001), aerobic stability (P = 0.010), and pH upward trend (P&lt;0.001). There was a decreasing linear effect on dry matter recovery (P&lt;0.001), pH (P&lt;0.001), ammonia nitrogen (P &lt;0.001), the maximum difference in the temperature of the silage in relation to the environment (P = 0.005) and maximum pH (P&lt;0.001) of silages. The inclusion of cactus pear affected the maximum temperature (P&lt;0.001) of silages in a quadratic way. There was a decreasing linear effect of dry matter (P&lt;0.001), ether extract (P&lt;0.001), Organic matter (P&lt;0,001), crude protein (P = 0,002) and neutral detergent fiber corrected for ash and protein (P = 0.030). The use of cactus pear promoted an increasing linear effect for the levels of mineral matter (MM) (P&lt;0.001), corresponding to an increase of 0,271%, for each 1% inclusion of cactus pear. The increase in the proportions of cactus pear in the silages promoted an increasing linear effect for mineral matter (P&lt;0,001). The inclusion of cactus pear by up to 60% in arboreal cotton silages alters the fermentation profile with increased fermentation losses and nutritional reduction. However, it still presents characteristics of good quality silages.

Enzymatic complex for broilers fed on a diet containing different levels of Distiller Dried Grains with Solubles

The objective of this research was to evaluate the effect of the inclusion of an enzyme complex (carbohydrases, proteases and phytase) in diets formulated with DDGS inclusion levels (0 to 30%) in parameters of performance, nutrient digestibility and serum biochemical profile of broiler chickens from 1 to 42 days old. The inclusion of DDGS impaired the performance characteristics in all phases, but broilers feeding with diets containing up to 8% DDGS improves the percentage carcass. Addition of the enzyme complex improved feed conversion by 3.6% and percentage of breast by 1.6%. At 42 days of age, there was a linear decreasing effect of the addition of DDGS in the diets on the relative weight of PV + gizzard (proventriculum + gizzard). Diets without enzyme addition decreased IDCDM, IDCCP and IDCNDF. Inclusion of DDGS up to 8 % results in maximum percentage carcass. The use of exogenous enzymes improves FCR (feed conversion ratio) in the pre-initial phase.

Flaxseed added to the diet of Alpine goats affects the nutrients intake and blood parameters.

The flaxseed is a nutraceutical food used as a source of α-linolenic acid, which can bring benefits to the health of mammals. This study was carried out to examine the effect of flaxseed inclusion in the diets on the intake of nutrients, body weight, and blood parameters of Alpine goats. Twenty-one adult females with an initial average weight of 41.06 ± 1.84kg were used in a completely randomized design, with four experimental treatments (0, 5, 10, and 15% of flaxseed in the total diet) and five replications per treatment. The intake of the dry matter presented a decreasing linear effect (P < 0.001), with a reduction of 53.5% between the control diet (0% of flaxseed) and 15% of flaxseed, but no effect was observed on weight gain (P > 0.05). Inclusion of flaxseed from 0 to 15% linearly decreased the intakes of organic matter, crude protein, non-fibrous carbohydrates, and neutral detergent fiber, but increased the ether extract intake (P < 0.001). Regarding plasma concentration traits, increasing the flaxseed levels from 0 to 15% had linear positive effect on LDL, VLDL, and triglycerides (P < 0.05), but no effect on cholesterol, HDL, glucose, creatinine, and urea (P > 0.05). The inclusion of flaxseed in the diet for 80days changes the nutrients intake and blood parameters but shows no impact on body weight. However, further studies are required to determine the impact of flaxseed on the goat's health in the long term since there has been an increase in the concentration of triglycerides, LDL, and VLDL.

Effect of replacing soybean meal with peanut meal on milk production and fat composition in lactating dairy cows.

The objective of this study was to evaluate the effects of replacing soybean meal with peanut meal on milk production, chemical and fatty acid composition, nutritional quality indicators of the lipid fraction, and the economic viability of the diets. Twelve crossbred cows in the initial lactation third, with a bodyweight of 545 ± 37kg and average milk production of 28kg/day of milk were distributed in a 4 × 4, triple Latin square design. The treatments consisted of diets with substitution levels of soybean meal for peanut meal (0; 330; 670; and 1000g/kg in DM). The peanut meal inclusion to replace soybean meal in the diets provided a decreasing linear effect for the protein (P = 0.02) and casein (P = 0.01) concentration in milk. Milk production, total solids concentration, feed efficiency, fatty acid composition, and nutritional quality indicators of the milk lipid fraction were not influenced by the substitution levels. The diet cost per kg DM decreased due to the peanut meal inclusion as a protein source. The partial or total substitution of soybean meal for peanut meal in the feedlot cows diet reduces the cost of feed, without affecting milk production and total solids yield.

Production aspects of hay of tropical pasture of

Context Tropical pasture have been little explored for haymaking, due to lack of information regarding loss of nutrients and of forage dehydration. Aims This study aimed to evaluate the production aspects of tropical pasture hays. Methods To characterise forage dry mass yield and dehydration rate, we studied the grasses Marandú, Xaraés, Massai, Paredão, Planaltina and Tupã. The water soluble carbohydrates concentration curve, chemical composition and in situ rumen degradability of dry mass, crude protein and neutral detergent fibre were also determined. Key results The highest forage dry mass yield was observed in 2018, when Planaltina and Paredão performed best (6.28 and 5.26 ± 0.41 t of dry matter ha−1, respectively). There was no genotype × dehydration time interaction (P = 0.81) on water soluble carbohydrates. Exposure to sun resulted in a linear decreasing effect (P &lt; 0.01) on water soluble carbohydrates content, with loss of 5.03 g kg−1 for each hour of exposure to the sun during the haymaking process. There was an interaction effect (P &lt; 0.01) between type of material and genotype on the content of dry matter, mineral matter, crude protein and neutral detergent fibre (P &lt; 0.01). In situ degradability of Massai grass dry mass was higher for the hay (758.0 and 253.8 ± 7.9 g kg−1 for hay and in natura material, respectively). Conclusions and implications The greater amount of leaves in relation to stem indicates that Massai grass is more efficient in dehydration during the first hours of exposure to the sun, as well as sustaining lower losses.

Production and economic analysis of milk from goats fed with double-distilled glycerin

The objective of this research was to evaluate the production, milk quality, consumption and financial analysis of milk from goats consuming diets with different levels of double-distilled glycerin. Twelve multiparous Saanen goats, weighing 40 ± 6 kg and 30 ± 5 days of lactation, kept in a confinement system for 60 days were used. The goats were randomly distributed in a Latin square (4 x 4), according to the levels of double-distilled glycerin. The ingredients used were tifton hay, ground corn grain, soybean meal, vitamin/mineral supplement, urea, and double-distilled glycerin levels (0, 6, 12 and 18%) to replace corn in the diets. The milk fat of goats fed with double-distilled glycerin in the diet showed a decreasing linear regressive effect (P = 0.001). Dry matter intake showed a quadratic regressive effect (P = 0.033). Evaluating the total cost of each diet, it was observed that the level containing 0% glycerin was the diet with the lowest cost per kilogram. The use of double-distilled glycerin in the feeding of dairy goats can be recommended up to the level of 18% of corn replacement without harming milk production, however, it is necessary to verify the economic viability, considering that there will be losses in the quality of the milk produced.

Influence of tannic acid on milk lipid composition of cows

The study aimed to evaluate tannic acid's influence on lactating cows' diets on milk production and lipid composition. Five crossbred cows (Holstein x Zebu), distributed in a 5 × 5 Latin square, were used to evaluate the effect of increasing tannic acid (hydrolyzable tannin). The diets were composed of 35 kg of corn silage as bulking and 6.38 kg of concentrate. The treatments consisted of increasing levels of tannic acid added to diets (0%, 1.3%, 2.6%, 3.9% and 5.2%). It was observed a decreasing linear effect (P<0.05) of the inclusion of tannic acid in the concentration of saturated butyric fatty acids (C4:0) presenting a mean of (2.99), and quadratic effect in the concentration of saturated fatty acids lauric (C12:0) presenting mean (26.74). The addition of tannic acid had effects on saturated fatty acids, decreasing the concentrations of butyric acid and increasing the concentrations of lauric acid. Atherogenicity and thrombogenicity rates reveal the potential for the prevention of coronary heart disease. However, the observed changes do not compromise the milk's chemical composition and nutritional quality,the y nutritional quality of the milk, presenting possibilities of human health benefits, thus enabling the aggregation of value for these products.

In vitro ruminal fermentation kinetics of diets with crambe cake protein replacing soybean meal protein by gas production technique

The objective of this study was to evaluate ingredients and diets containing increasing levels of crambe cake protein replacing soybean meal protein, with in vitro ruminal fermentation parameters using a gas production technique. Diets were formulated for feedlot lambs and contained different levels of crambe cake protein (0, 250, 500, 750, and 1000 g kg-1) replacing soybean meal protein. Corn silage was used as roughage. Carbohydrate digestion rates were estimated using the in vitro gas production technique and the cumulative gas production kinetics were analyzed using the bicompartmental logistic model. The parameters values of ruminal degradation kinetics were generated using the R statistical program with the Gauss-Newton algorithm and then subjected to analysis of variance and regression (when necessary) according to a completely randomized experimental design with five treatments and five replications. Upon carbohydrate fractionation of ingredients and experimental diets, it was observed that corn grain and corn silage presented the highest levels of total carbohydrates (TC), with values of 128.3 and 464.8 g kg-1 dry matter (DM) in fraction B2, respectively. Lower TC content was found for soybean meal and crambe cake (CC). There was a predominance of fractions A + B1 in the ingredients and experimental diets. The B2 fraction decreased in the diets with the inclusion of the CC protein, and CC presented the highest C fraction. Protein fractionation (g kg-1 DM and g kg-1 crude protein - CP), the ingredients and diets showed a higher proportion of fractions A and B1 + B2. In in vitro degradation, the diet without CC (0 g kg-1 DM) showed the highest final cumulative gas production (365.04 mL g-1 of incubated DM), while the CC presented the lowest volume (166.68 mL g-1 of incubated DM). The gas volume of non-fibrous carbohydrate fermentation and fibrous carbohydrate degradation rate exhibited a quadratic effect according increasing levels of CC (Pmax = 265.8 g kg-1 DM and Pmin = 376.3 g kg-1 DM, respectively). The lag time and final gas volume showed a decreasing linear effect with increasing levels of CC protein. The degradation rate of non-fibrous carbohydrates and the final volume of fibrous carbohydrates did not differ. Replacing soybean meal protein with CC protein at the level of 250 g kg-1 of dry matter in diets formulated for feedlot lambs leads to good profiles of ruminal fermentation kinetics with respect to the degradation of fibrous and non-fibrous carbohydrates.