Physicochemical and microbiological parameters of rumen fluid of lactating goats fed diets containing crude glycerin from waste frying oil used in biodiesel production.

Abstract

Crude glycerin from the biodiesel industry is a alternative energy source used to replace part of the energy components of the diet, since the nutritional value of glycerol makes it suitable for use in ruminant diets. The objective was to analyze the effects including crude glycerin (CG) derived from the production of biodiesel from residual frying oil, in diets of lactating goats on the physicochemical and microbiological parameters of rumen fluid. Eight 2-year-old goats weighting approximately 42.06 ± 3.5kg of body weight (BW) were kept in individual stalls, receiving diets containing 0, 7, 14 and 21% of CG (% DM). The experiment was carried out according to a double 4 × 4 Latin square and lasted for 80days. Orthogonal contrasts were tested to determine linear and quadratic effects, as well as the effects of 0% CG in the diet in comparison to the other treatments. There was a linear decreasing effect on DM intake (P = 0.001) and neutral detergent fiber (P = 0.0001), as well as a quadratic effect (P = 0.0002) on ether extract (EE) intake with increasing level of CG in the diet. Including CG affected motility and density of the protozoa (P = 0.0001). Treatments had no effect on pH, N-NH3 concentration and percentage of protozoa in the rumen liquid. There was increasing linear effect on sedimentation and flotation time (P = 0.001). Therefore, including 14 and 21% CG in the diet as a source of glycerol decreased nutrient intake and altered the ruminal microbiota, but pH and N-NH3 were not affected. Thus, to avoid impairment to the physicochemical and microbiological parameters of the rumen liquid of goats, the inclusion of CG should not exceed 7% DM.