Decrease In Soil Water Content Research Articles

Four decades of soil water stress history together with host genotype constrain the response of the wheat microbiome to soil moisture.

There is little understanding about how soil water stress history and host genotype influence the response of wheat-associated microbiome under short-term decreases in soil moisture. To address this, we investigated how plant breeding history (four wheat genotypes; two with recognized drought resistance and two without) and soil water stress history (same wheat field soil from Saskatchewan with contrasting long-term irrigation) independently or interactively influenced the response of the rhizosphere, root and leaf bacterial and fungal microbiota to short-term decreases in soil water content (SWC). We used amplicon sequencing (16S rRNA gene for bacteria and ITS region for fungi) to characterize the wheat microbiome. Fungal and bacterial communities responses to short-term decreases in SWC were mainly constrained by soil water stress history, with some smaller, but significant influence of plant genotype. One exception was the leaf-associated fungal communities, for which the largest constraint was genotype, resulting in a clear differentiation of the communities based on the genotype's sensitivity to water stress. Our results clearly indicate that soil legacy does not only affect the response to water stress of the microbes inhabiting the soil, but also of the microorganisms more closely associated with the plant tissues, and even of the plant itself.

Time-lapse monitoring of root water uptake using electrical resistivity tomography and mise-à-la-masse: a vineyard infiltration experiment

Abstract. This paper presents a time-lapse application of electrical methods (electrical resistivity tomography, ERT; and mise-à-la-masse, MALM) for monitoring plant roots and their activity (root water uptake) during a controlled infiltration experiment. The use of non-invasive geophysical monitoring is of increasing interest as these techniques provide time-lapse imaging of processes that otherwise can only be measured at few specific spatial locations. The experiment here described was conducted in a vineyard in Bordeaux (France) and was focused on the behaviour of two neighbouring grapevines. The joint application of ERT and MALM has several advantages. While ERT in time-lapse mode is sensitive to changes in soil electrical resistivity and thus to the factors controlling it (mainly soil water content, in this context), MALM uses DC current injected into a tree stem to image where the plant root system is in effective electrical contact with the soil at locations that are likely to be the same where root water uptake (RWU) takes place. Thus, ERT and MALM provide complementary information about the root structure and activity. The experiment shows that the region of likely electrical current sources produced by MALM does not change significantly during the infiltration time in spite of the strong changes of electrical resistivity caused by changes in soil water content. Ultimately, the interpretation of the current source distribution strengthened the hypothesis of using current as a proxy for root detection. This fact, together with the evidence that current injection in the soil and in the stem produces totally different voltage patterns, corroborates the idea that this application of MALM highlights the active root density in the soil. When considering the electrical resistivity changes (as measured by ERT) inside the stationary volume of active roots delineated by MALM, the overall tendency is towards a resistivity increase during irrigation time, which can be linked to a decrease in soil water content caused by root water uptake. On the contrary, when considering the soil volume outside the MALM-derived root water uptake region, the electrical resistivity tends to decrease as an effect of soil water content increase caused by the infiltration. The use of a simplified infiltration model confirms at least qualitatively this behaviour. The monitoring results are particularly promising, and the method can be applied to a variety of scales including the laboratory scale where direct evidence of root structure and root water uptake can help corroborate the approach. Once fully validated, the joint use of MALM and ERT can be used as a valuable tool to study the activity of roots under a wide variety of field conditions.

Effects of Water Stress on Fluorescence Parameters and Photosynthetic Characteristics of Drip Irrigation in Rice

To study the effects of water stress on the fluorescence parameters and photosynthetic characteristics of rice under drip irrigation and mulching, so as to determine the response mechanisms to water stress during the tillering stage. A two-year trial was carried out at Shihezi University, China. Three water gradients were investigated. The results showed that the chlorophyll content (a + b), photosynthetic rate (Pn), and leaf area index (LAI) decreased with decreasing soil moisture content at the tillering stage. The chlorophyll content (a + b) and Pn in the flooding irrigation (CK) treatment were significantly higher than those in the stress treatments, and the chlorophyll content (a + b) and Pn in the W1 and W2 treatments were significantly lower than those in the other treatments. The maximum LAI of the CK, W1, and W2 treatments were similar, while the W3 produced lower values; stress treatment improved the ability of tillering in the early and middle stages, while the decrease in soil water content in the tillering stage resulted in a decrease in the final tillering rate; drought stress in the tillering stage resulted in decreased rice yields. The yield of the W1 and W2 treatments were similar, while that of the W3 treatment was seriously reduced. The main reasons for the reduction in yield was the significant decrease in the number of effective panicles, the seed setting rate, and a decrease in the 1000-grains weight. Water consumption in the stress treatments decreased by 51.69%–58.78% compared to the CK treatment; water-use efficiency in the CK treatment was only 0.25 kg·m−3, and the water-use efficiency of the stress treatments increased by 40%–72%. We should make full use of the compensation effect of drought stress in the water regulation of drip irrigation in covered rice and adopt the water control measure of the W2 treatment in the tillering stage. These measures are conducive to improving water-use efficiency and achieving the goal of high quality, high yield, and high efficiency.

Early effects of temperate agroforestry practices on soil organic matter and microbial enzyme activity

A field experiment was conducted to evaluate the effects of alley cropping systems on microbial activity and soil organic matter (SOM) pools. We hypothesized that enzyme activity and labile pools of SOM are early and sensitive indicators of changes induced by tree introduction in the cropping systems. Poplar-alfalfa and alder-gramineous (cereal or ryegrass) associations and their respective control systems (alfalfa and gramineous) were compared in terms of soil carbon (C), nitrogen (N) and water contents, SOM labile pools, NIRS-MIRS spectra and microbial enzyme activity in the topsoil (0–15 cm) for 4 years after tree planting. After 1 year, tree introduction induced a decrease in soil water content, microbial biomass N and some enzyme activities under alfalfa system. After 4 years, tree introduction resulted in higher soil water contents in both systems (alfalfa and gramineous); higher microbial biomass N and lower C:N in alfalfa-poplar plots compared to control plots. MIRS-NIRS analyses showed a greatest differentiation in SOM quality between alfalfa-based systems. The effects of temperate agroforestry systems on SOC in the topsoil are relatively weak in the first years after tree introduction. Observed effects were more pronounced in the alfalfa-poplar system, probably due to higher tree growth. Further studies will provide insights into the longer-term effects of these systems on soil functioning.

Water scaling of ecosystem carbon cycle feedback to climate warming.

It has been well established by field experiments that warming stimulates either net ecosystem carbon uptake or release, leading to negative or positive carbon cycle-climate change feedback, respectively. This variation in carbon-climate feedback has been partially attributed to water availability. However, it remains unclear under what conditions water availability enhances or weakens carbon-climate feedback or even changes its direction. Combining a field experiment with a global synthesis, we show that warming stimulates net carbon uptake (negative feedback) under wet conditions, but depresses it (positive feedback) under very dry conditions. This switch in carbon-climate feedback direction arises mainly from scaling effects of warming-induced decreases in soil water content on net ecosystem productivity. This water scaling of warming effects offers generalizable mechanisms not only to help explain varying magnitudes and directions of observed carbon-climate feedback but also to improve model prediction of ecosystem carbon dynamics in response to climate change.

Spatial variation of correlations between vertical soil water and evapotranspiration and their controlling factors in a semi-arid region

A decrease in soil water content (SWC) has been observed at the global scale and has led to a reduction in evapotranspiration (ET). However, the trend in SWC in eastern Asia differed from the global trend, and this may be due to the overlapping of warm/wet seasons. Because of the limitation of deep soil water content data, especially in drylands, changes in SWC and the correlation between SWC (particularly deep SWC) and ET remain poorly understood. In this study, spatial distribution of correlation coefficient (r) between SWC and ET were calculated based on reanalysis data and remote sensing data, which were validated by in situ data (2004–2005) and sampling data (2016–2017). Firstly, the correlation between SWC and ET decreased with soil depth. The depth of the sharp decrease was 10 cm based on in situ monitoring data (2005–2008) and spatial sampling data (2016–2017). Secondly, the spatial correlation between SWC and ET varied with different vegetation cover types. The correlation increased from the western typical steppe to the eastern forest. The highest values of r were in a forest-covered area, because it had the highest water uptake capacity. However, the difference between r_10 and r_100 (D_r) showed a different spatial pattern, and the lowest negative D_r value was obtained from a forest–grass transition zone in which the enhanced vegetation index showed a significant increasing trend. The significant negative regression determination coefficient (R2 = 0.9, p < 0.01) between B_EVI (0–0.1) and D_r indicated that the narrowed D_r in the transition zone was due to the vegetation increase. This meant that more deep soil water was taken up into the atmosphere. Thus, the deep SWC could become unstable and deep soil could become drier, which would be unsustainable for the ecosystem.

Scheduling deficit subsurface drip irrigation of apple trees for optimizing water use

The impact of four different irrigation strategies on soil and plant water status and fluxes of photosynthesis (A) and chamber transpiration (E) has been studied under continental climate on field-grown apple trees (Malus domestica ‘Gala’) in 2010 and 2011. The non-irrigated treatment resulted in a decrease in soil water content (SWC) throughout the season in comparison with irrigated treatments. Non-irrigated trees showed more negative values of predawn leaf water potential (ΨP), which decreased to − 0.2 MPa, reflecting a moderate water stress during the fruit ripening period. A decrease (15 to 25%) of stomatal conductance (gs), leaf photosynthesis (A), and transpiration (E) were observed in these water-stressed trees (T1). Well-irrigated trees during the whole season (T2) showed the highest rates of gs, A, E, as well as of ΨP values, which were around − 0.1 MPa. Trees irrigated only during the fruit cell division period (stage I) and the ripening period (stage III) (T3) showed a small decrease (10%) in leaf gas exchanges. A regulated deficit irrigation (RDI) applied only during the fruit cell growth (stage II) on trees and well-irrigated during the fruit cell division period and the ripening period (T4) seems to have little effects on gas exchanges and no effects on yield and fruit quality before and after storage. Therefore, the results showed that with the exception of RDI treatment, increasing water stress reduced the physiological parameters due to stomatal and non-stomatal limiting factors. However, such regulated deficit irrigation saved 45% of water compared to the comfort irrigation. Considering these results, regulated deficit subsurface drip irrigation applied during the fruit growth II is a sustainable strategy for saving water, increasing water use efficiencies, and preserving the physicochemical quality of the apple fruits before and after storage.

Arabidopsis species deploy distinct strategies to cope with drought stress.

Background and AimsWater limitation is an important determinant of the distribution, abundance and diversity of plant species. Yet, little is known about how the response to limiting water supply changes among closely related plant species with distinct ecological preferences. Comparison of the model annual species Arabidopsis thaliana with its close perennial relatives A. lyrata and A. halleri, can help disentangle the molecular and physiological changes contributing to tolerance and avoidance mechanisms, because these species must maintain tolerance and avoidance mechanisms to increase long-term survival, but they are exposed to different levels of water stress and competition in their natural habitat.MethodsA dry-down experiment was conducted to mimic a period of missing precipitation. The covariation of a progressive decrease in soil water content (SWC) with various physiological and morphological plant traits across a set of representative genotypes in A. thaliana, A. lyrata and A. halleri was quantified. Transcriptome changes to soil dry-down were further monitored.Key ResultsThe analysis of trait covariation demonstrates that the three species differ in the strategies they deploy to respond to drought stress. Arabidopsis thaliana showed a drought avoidance reaction but failed to survive wilting. Arabidopsis lyrata efficiently combined avoidance and tolerance mechanisms. In contrast, A. halleri showed some degree of tolerance to wilting but it did not seem to protect itself from the stress imposed by drought. Transcriptome data collected just before plant wilting and after recovery corroborated the phenotypic analysis, with A. lyrata and A. halleri showing a stronger activation of recovery- and stress-related genes, respectively.ConclusionsThe response of the three Arabidopsis species to soil dry-down reveals that they have evolved distinct strategies to face drought stress. These strategic differences are in agreement with the distinct ecological priorities of the stress-tolerant A. lyrata, the competitive A. halleri and the ruderal A. thaliana.

Effect of Irrigation Water Regimes on Yield of Tetragonia Tetragonioides

The main purpose of this experiment was to study the effect of several irrigation water regimes on Tetragonia tetragonioides (Pall) O. Kuntze in semi-arid regions. During the experiment period, it was measured that several irrigation regimes were affected in terms of growth, biomass production, total yield, mineral composition, and photosynthetic pigments. The experiment was conducted in the greenhouse at the University of Algarve (Portugal). The study lasted from February to April in 2010. Three irrigation treatments were based on replenishing the 0.25-m-deep pots to field capacity when the soil water level was dropped to 70% (T1, wet treatment), 50% (T2, medium treatment), and 30% (T3, dry treatment) of the available water capacity. The obtained results showed that the leaf mineral compositions of chloride and sodium, the main responsible ions for soil salinization and alkalization in arid and semi-arid regions, enhanced with the decrease in soil water content. However, the minimum amounts of chlorophyll, carotenoids, and soluble carbohydrates in the leaf content were obtained in the medium and driest treatments. On the other hand, growth differences among the several irrigation regimes were very low, and the crop yield increased in the dry treatment compared to the medium treatment; thus, the high capacity of salt-removing species suggested an advantage of its cultivation under dry conditions.

Scotch broom (Cytisus scoparius) modifies microenvironment to promote nonnative plant communities

Scotch broom [Cytisus scoparius (L.) Link] is a globally important nitrogen (N)-fixing invasive plant species that has potential to alter soil water dynamics, soil chemistry, and plant communities. We evaluated the effects of Scotch broom on soil moisture, soil chemistry, soil temperature, photosynthetically active radiation (PAR), and vegetation communities over 4 years at a site recently harvested for timber. Treatments of Scotch broom (either present via planting or absent) and background vegetation (either present or absent via herbicide treatments) were applied to 4 m2 plots. Background vegetation was associated with the greatest decrease of soil water content (SWC) among treatments. During the driest year, Scotch broom showed some evidence of increased early-and late-season soil water usage, and, briefly, a high usage relative to background vegetation plots. On a percent cover basis, Scotch broom had a substantially greater negative influence on SWC than did background vegetation. Surprisingly, Scotch broom was not consistently associated with increases in total soil N, but there was evidence of increasing soil water N when Scotch broom was present. Scotch broom-only plots had greater concentrations of soil water magnesium (Mg2+) and calcium (Ca2+) than other treatments. On a percent cover basis, Scotch broom had a uniquely high demand for potassium (K+) relative to the background vegetation. Average soil temperature was slightly greater, and soil surface PAR lower, with Scotch broom present. Scotch broom-absent plots increased in species diversity and richness over time, while Scotch broom-present plots remained unchanged. Scotch broom presence was associated with an increase in cover of nonnative sweet vernalgrass (Anthoxanthum odoratum L.). Scotch broom generated positive feedbacks with resource conditions that favored its dominance and the establishment of nonnative grass.

An experimental irrigation tool for creating a water gradient across soil depths under terminated rainfall conditions

An experimental irrigation tool to research the response of plants to soil drying from deep to shallow soil layers observed under limited rainfall conditions was tested. Water was supplied from the tips of 4-mm-diameter tubes measuring 0.1, 0.3, 0.6, and 0.85 m in the length inserted into the soils. Each tube was set from shallowest to deepest at intervals of 1.5–3.0 m in the field. Control by the irrigation system was evaluated in rice and spring wheat fields under rain-protected shelters, where two cultivars with different root depths were planted. The irrigation tool created a series of different soil water profiles in which wet soil layers decreased with increasing irrigation depth due to the inserted tube lengths in both fields. In fine sandy soils more than sand, continuous decreases in soil water content were well simulated. Leaf conductance, leaf green color (SPAD value), and aboveground plant dry weight in both fields decreased with increasing irrigation depth in shallow root cultivars to a greater extent than that in deep root cultivars. The lower reducing effect of deep irrigation on plant dry weight in wheat compared to that in rice suggested that the irrigation depth based on tube length should be changed depending on the root distribution traits in the tested crops. These results indicated that the irrigation tool can conveniently simulate a diverse and continuous soil water content profile closer to that of an actual water gradient under limited rainfall conditions and contribute to studies on crop responses to different soil water absorption traits.

Effect of Mesoscale Land Use Change on Characteristics of Convective Boundary Layer: Semi-Idealized Large Eddy Simulations over Northwest China

Although large-scale topography and land use have been properly considered in weather and climate models, the effect of mesoscale and microscale heterogeneous land use on convective boundary layer (CBL) has not been fully understood yet. In this study, the influence of semi-idealized strip-like patches of oases and deserts, which resemble irrigated land use in Northwest China, on the CBL characteristics, is investigated based on the Weather Research and Forecasting (WRF)-large eddy simulation (LES) driven by observed land surface data. The influences of soil water content in oases on aloft CBL flow structure, stability, turbulent kinetic energy (TKE), and vertical fluxes are carefully examined through a group of sensitivity experiments. The results show that secondary circulation (SC)/turbulent organized structures (TOS) is the strongest/weakest when soil water content in oases is close to saturation (e.g., when the oases are irrigated). With the decrease of soil water content in oases (i.e., after irrigation), SC (TOS) becomes weak (strong) in the lower and middle CBL, the flux induced by SC and TOS becomes small (large), which has a dramatic impact on point measurement of eddy covariance (EC) fluxes. The flux induced by SC and TOS has little influence on EC sensible heat flux, but great influence on EC latent heat flux. Under this circumstance, the area averaged heat flux cannot be represented by point measurement of flux by the EC method, especially just after irrigation in oases. Comparison of imbalance ratio (i.e., contribution of SC and TOS to the total flux) reveals that increased soil moisture in oases leads to a larger imbalance ratio as well as enhanced surface heterogeneity. Moreover, we found that the soil layer configuration at different depths has a negligible impact on the CBL flux properties.

Hubungan antara Neraca Air Lahan dan Produksi Karet Klon PB260

In general, rubber production was influenced by fluctuation of monthly rainfall rate. This research was aimed to determine the effect of rainfall, evapotranspiration, and soil water content on the production of PB260 rubber clone. This research was conducted at Sembawa Research Centre Experimental Field, South Sumatera from the year 2013-2016. The observed rubber tree was mature PB260 clone planted on clay loam soil. Rubber productions on the dry and rainy season on 2013-2016 were compared to determine the effect of rainfall on rubber production. The results showed that soil water content has the closest relationship to rubber production compared to rainfall and evapotranspiration parameter. Latex yield was decreasing as the decrease of soil water content at dry season. Limited water during the dry season became a constraint of optimum rubber yield. When soil water content drops below 100 mm, the maximum attained latex yield was 18 g/t/t.

Interpreting in situ Soil Water Characteristics Curve under Different Paddy Soil Types Using Undisturbed Lysimeter with Soil Sensor

The soil water characteristics curve (SWCC) represents the relation between soil water potential and soil water content. The shape and range of SWCC according to the relation could vary depending on soil characteristics. The objective of the study was to estimate SWCC depending on soil types and layers and to analyze the trend among them. To accomplish this goal, the unsaturated three soils were considered: silty clay loam, loam, and sandy loam soils. Weighable lysimeters were used for exactly measuring soil water content and soil water potential. Two fitting models, van Genuchten and Campbell, were applied. Two models entirely fitted well the measured SWCC, indicating low RMSE and high R2 values. However, the large difference between the measured and the estimated was found at the 30 cm layer of the silty clay loam soil, and the gap was wider as soil water potential increased. In addition, the non-linear decrease of soil water content according to the increase of soil water potential tended to be more distinct in the sandy loam soil and at the 10 cm layer than in the silty clay loam soil and at the lower layers. These might be seen due to the various factors such as not only pore size distribution, but also cracks by high clay content and plow pan layers by compaction. This study clearly showed difficulty in the estimation of SWCC by such kind of factors. Parameters obtained from the Campbell and van Genuchten models and goodness-of-fit results.

Dynamic niche partitioning in root water uptake facilitates efficient water use in more diverse grassland plant communities

Abstract Efficient extraction of soil water is essential for the productivity of plant communities. However, research on the complementary use of resources in mixed plant communities, and especially the impact of plant species richness on root water uptake, is limited. So far, these investigations have been hindered by a lack of methods allowing for the estimation of root water uptake profiles. The overarching aim of our study was to determine whether diverse grassland plant communities in general exploit soil water more deeply and whether this shift occurs all the time or only during times of enhanced water demand. Root water uptake was derived by analysing the diurnal decrease in soil water content separately at each measurement depth, thus yielding root water uptake profiles for 12 experimental grasslands communities with two different levels of species richness (4 and 16 sown species). Additional measurements of leaf water potential, stomatal conductance, and root traits were used to identify differences in water relations between plant functional groups. Although the vertical root distribution did not differ between diversity levels, root water uptake shifted towards deeper layers (30 and 60 cm) in more diverse plots during periods of high vapour pressure deficit. Our results indicate that the more diverse communities were able to adjust their root water uptake, resulting in increased water uptake per root area compared to less diverse communities (52% at 20 cm, 118% at 30 cm, and 570% at 60 cm depth) and a more even distribution of water uptake over depth. Tall herbs, which had lower leaf water potential and higher stomatal conductance in more diverse mixtures, contributed disproportionately to dynamic niche partitioning in root water uptake. This study underpins the role of diversity in stabilizing ecosystem function and mitigating drought stress effects during future climate change scenarios. Furthermore, the results provide evidence that root water uptake is not solely controlled by root length density distribution in communities with high plant diversity but also by spatial shifts in water acquisition. A plain language summary is available for this article.

Water sources of Nitraria sibirica and response to precipitation in two desert habitats

Nitraria sibirica usually exists in a form of nebkhas, and has strong ecological adaptability. The plant species has distinctive function for wind prevention and sand fixation, and resistance drought and salt. However, the water condition is still a limiting factor for the plant survival and development. In order to understand the water use strategy of the plant in different desert habitats, we selected the N. sibirica growing in sandy desert habitat and gravel desert habitat to study the seaso-nal variation of plant water sources and response to precipitation at the edge of the oasis of Linze in the Hexi Corridor. We measured the oxygen stable isotope of the plant stem water and the different potential water sources (precipitation, soil water and ground water), and used the IsoSource model to calculate the proportion of water sources from the potential water. The results showed that there were significant seasonal variation characteristics of δ18O value and water source of stem water for the plant in the two habitats. In the sandy habitat, the plant used more ground water in the less precipitation seasons including spring and fall, and more than 50% of the water sources absorbed from ground water. However, under the condition of gravel habitat, the plant could not achieve the ground water level depth of 11.5 m, and its water source was controlled by precipitation, which had large seasonal variability. The water sources of N. sibirica had significant responses to the change of precipitation in the two desert habitats. Following the rapid decrease of soil water content after the precipitation events, the plant in the sandy habitat turned to use the abundant ground water as the main sources of water, while the plant in the gravel habitat only used the less water from precipita-tion infiltration to the deep soil. Therefore, different water use strategies of the plant in the two habitats were the main reason for the difference in growth characteristics, and it had a strong ability of self-adjustment and adaptation.

Physiological responses of five plants in northwest China arid area under drought stress

Effects of drought stress on photosynthetic characteristics, water status, physiological and biochemical indexes were studied in five arid area plants in northwest China, including Potentilla fruticosa, Lycium ruthenicum, Caryopteris mongholica, Caragana korshinskii and Hedysarum scoparium, using pot experiments. The results showed that with the prolongation of drought stress, the water conservation capacity of leaves totally increased in above five plants, the leaf relative water content rose first and then fell, and the relative chlorophyll content and chlorophyll fluorescence parameters decreased to some extent. Overall, the photosynthetic system of H. scoparium and L. ruthenicum least affected by the stress, and P. fruticosa suffered with the most serious damage. The electric conductivity and malondialdehyde (MDA) content of P. fruticosa firstly decreased and then sharply increased, while superoxide dismutase (SOD) and peroxidase (POD) activities firstly increased and then dramatically decreased. With the decrease of soil water content, the electric conductivity rose firstly and then fell in the other four plants,the change of MDA content rose first and then fell, or fell first and then rose, and the activities of SOD and POD almost displayed upward trend. Especially, SOD activity of C. mongholica and POD activity of H. scoparium had the biggest increment among the five plants after 28 days of drought stress, increasing by 1.1 and 13.1 times respectively, compared with before processing. Additionally, there was significant variance in the accumulation of organic solutes among the five plants during the drought stress treatment. The accumulation of soluble sugar and free proline was the most in P. fruticosa, increasing by 1.1 and 22.4 times respectively, after 28 days of drought treatment. H. sco-parium accumulated the most soluble protein, increasing by 1.0 times after 28 days of drought treatment. The result of membership function method showed that the drought tolerance decreased in order of H. scoparium ＞ L. ruthenicum ＞ C. korshinskii ＞ C. mongholica ＞ P. fruticosa. The research revealed that all five plants could make positive response to drought stress through improving the activity of antioxidant enzymes and accumulating organic solutes, which could help to reduce cell damage. P. fruticos was most sensitive to water deficit in the five plants, and the degree of drought had exceeded its own regulated capacity threshold during the experiment.

Modelling hydraulic functioning of an adult beech stand under non-limiting soil water and severe drought condition

Modelling hydraulic functioning of a forest stand is a prerequisite to predict the future impact of climate change on forests. In this paper, we used a process-based model of the soil-plant-atmosphere continuum to investigate the links between energy budget and water balance, and to emphasize the key processes involved in the control of transpiration and water status of forest trees. The model describes stomatal conductance as a function of photosynthesis, intercellular CO2 concentration and leaf water potential. The latter in turn depends on soil and tree storage water potentials, the water flux through the soil and the trees, hydraulic resistances and stomatal conductance. We have implemented in the model a detailed tree water storage scheme, canopy interception of precipitation, and the rate of change of forest canopy energy storage. In this model, physical climate processes and ecological processes are closely coupled which involves important interactions and feedbacks. The model reproduces the observed variation in leaf water potential in dry and wet conditions. It successfully captures the decrease in soil water content under both non-limiting soil water and severe drought conditions and there is a good agreement between measured and simulated sensible and latent heat fluxes throughout the season. Simulations also show that significant amounts of intercepted water can be lost through evaporation during rain events. The results corroborate that the concept of hydraulic capacitance provides a simple and effective means of simulating the buffering action of tree water storage on tree water status. The two key parameters that control transpiration and water status of the trees are xylem hydraulic resistances and sensitivity of stomata to leaf water potential. The results confirm that stomatal conductance cannot be modelled using leaf-level processes alone, but must be incorporated into a comprehensive model of water flow from soil through the plants to the atmosphere where various self-regulation are set up to ensure a complete water status equilibrium.

Physiological and biochemical responses of Festuca sinensis seedlings to temperature and soil moisture stress.

The interaction effects between temperature and soil moisture on Festuca sinensis Keng ex E.B.Alexeev were analysed to determine how F. sinensis responds to these environmental conditions. A pot experiment was conducted in a greenhouse under simulated growth conditions with four soil moisture contents (80, 65, 50 and 35% relative saturation moisture content) and three temperature conditions (15, 20 and 25°C). Physiological (relative water content and root activity) and biochemical parameters (chlorophyll, peroxidase (POD), malondialdehyde (MDA), soluble protein, soluble sugar and free proline) were evaluated at the seedling stage. Results showed that with a decrease in soil water content, the POD activities, MDA content, soluble protein content, soluble sugar content and free proline content of plants under the 15°C and 20°C treatments initially decreased and then increased, whereas they increased with a decrease of soil water content at 25°C. The relative water contents of plants under the three temperature treatments decreased with a decreasing soil moisture content, but then increased temperature significantly reduced the relative water content of the seedlings under low soil water content. The chlorophyll contents of plants under the 25°C treatment decreased with a decrease of soil moisture content, but those of plants under the 15°C and 20°C treatments initially increased and then decreased. The root activities of plants under the 15°C and 20°C treatments increased with a decreasing soil moisture content; however, those of plants under the 25°C treatment initially increased and then decreased. Thus, results indicated that changes of temperature and soil moisture content had significant and complicated effects on the physiological-biochemical characteristics of F. sinensis; the conditions of 20°C and 65% RSMC had positive effects on F. sinensis seedling growth and the appropriate drought stress could promote the growth of seedling roots under the three different temperature conditions. In conclusion, F. sinensis seedlings could adapt to certain changes in the ecological environment by regulating their physiological and biochemical reactions.

Responses of plant community structure and species composition to warming and N addition in an alpine meadow, northern Tibetan Plateau, China

Global climate warming and increasing nitrogen (N) deposition, as controversial global environmental issues, may distinctly affect the functions and processes of terrestrial ecosystems. It has been reported that the Qinghai-Tibet Plateau has been experiencing significant warming in recent decades, especially in winter. Previous studies have mainly focused on the effects of warming all the year round; however, few studies have tested the effects of winter warming. To investigate the effects of winter warming and N addition on plant community structure and species composition of alpine meadow, long-term N addition and simulated warming experiment was conducted in alpine meadow from 2010 in Damxung, northern Tibet. The experiment consisted of three warming patterns: Year-round warming (YW), winter warming (WW) and control (NW), crossed respectively with five N gradients: 0, 10, 20, 40, 80 kg N·hm-2·a-1. From 2012 to 2014, both warming and N addition significantly affected the total coverage of plant community. Specifically, YW significantly decreased the total coverage of plant community. Without N addition, WW remarkably reduced the vegetation coverage. However, with N addition, the total vegetation coverage gradually increased with the increase of N level. Warming and N addition had different effects on plants from different functional groups. Warming significantly reduced the plant coverage of grasses and sedges, while N addition significantly enhanced the plant coverage of grasses. Regression analyses showed that the total coverage of plant community was positively related to soil water content in vigorous growth stages, indicating that the decrease in soil water content resulted from warming during dry seasons might be the main reason for the decline of total community coverage. As soil moisture in semi-arid alpine meadow is mainly regulated by rainfalls, our results indicated that changes in spatial and temporal patterns of rainfalls under the future climate change scenarios would dramatically influence the vegetation coverage and species composition. Additionally, the effects of increasing atmospheric N deposition on vegetation community might also depend on the change of rainfall patterns.