Decreased Glutathione Peroxidase Activity Research Articles

JNK inhibitor and ferroptosis modulator as possible therapeutic modalities in Alzheimer disease (AD)

Alzheimer disease (AD) is among the most prevalent neurodegenerative diseases globally, marked by cognitive and behavioral disruptions. Ferroptosis is a form of controlled cell death characterized by intracellular iron accumulation associated with lipid peroxide formation, which subsequently promotes AD initiation and progression. We hypothesized that targeting the ferroptosis pathway may help in AD management. Therefore, our study aimed to evaluate the potential neuroprotective effect of the antifungal Ciclopirox olamine (CPX-O) that acts through iron chelation. We employed CPX-O separately or in combination with the JNK inhibitor (SP600125) in a mice model of AlCl3-induced AD. Animals underwent examination for behavioral, biochemical, histological, and immunohistochemical findings. Our results revealed that AlCl3 was associated with disruptions in learning and memory parameters, neuronal degeneration in the hippocampus, increased immunoreactivity of amyloid-β and tau proteins, a significant rise in iron, nitric oxide (NO), malondialdehyde (MDA), JNK, and P53 levels, along with the significant decrease in glutathione peroxidase activity. Interestingly, the administration of CPX-O alone or in combination with SP600125 in the AlCl3-induced AD model caused an improvement in the previously described examination findings. Therefore, CPX-O may be a promising candidate for AD treatment, and future clinical trials will be required to confirm these preclinical findings.

Reduced Glutathione Peroxidase (GPx) Activity in Patients with Cutaneous Leishmaniasis

Background: Cutaneous leishmaniasis (CL) is a tropical parasitic infection that involves several factors in its pathogenesis, including oxidative stress. The oxidative stress in CL may deplete antioxidant nutrients. Objectives: The aim of the study was to measure glutathione peroxidase (GPx) activity in patients with CL. Methods: The investigation included 80 patients with CL and 80 healthy participants. A diagnostic kit was used to measure GPx activity. Results: The results showed a significant decrease in GPx activity in the CL patient group compared to the control group (P < 0.001), which is related to increased oxidative stress in patients. Conclusions: According to the results, GPx enzyme activity can serve as a biomarker for the diagnosis, prognosis, and monitoring of treatment in patients with CL.

Radioprotective effects of linden honey in rat peripheral blood

Radiotherapy affects not only malignant, but also a healthy tissue adjacent to tumor by increasing reactive oxygen species generation, with consequent damage to biomolecules, such as the oxidation of membrane lipids, known as lipid peroxidation. The end product of lipid peroxidation is malondialdehyde. Radioprotectors are compounds that could significantly protect normal cells from radiation, without changing the tumor cell radiosensitivity. Synthetic radioprotectors usually have side effects and are toxic. Natural radioprotectors exert protection without adverse effects. In this study, we examined the radioprotective ability of linden honey in rat blood, by detecting alterations in the activities of antioxidant enzymes catalase and glutathione peroxidase and malondialdehyde concentration after the exposure to a therapeutic dose of gamma rays. Sixteen rats were randomly divided into Control and Honey groups. Honey group received honey (1.5 mL(kgd-1)) orally for four weeks, while at the same time Control group were given distilled water. After four weeks, blood was sampled from all animals. Samples were halved, and one series of samples were gamma irradiated (2 Gy). Radiation induced decreased glutathione peroxidase activity and increased malondialdehyde level, while honey treatment attenuated those alterations, keeping glutathione peroxidase and malondialdehyde at physiological levels. These findings confirm radioprotective properties of linden honey.

The effect of enterocin A/P dipeptide on growth performance, glutathione-peroxidase activity, IgA secretion and jejunal morphology in rabbits after experimental methicillin-resistant Staphylococcus epidermidis P3Tr2a Infection

The increasing frequency of methicillin-resistant (MR) staphylococci in humans and animals need special attention for their difficult treatment and zoonotic character, therefore novel antimicrobial compounds on a natural base against antibiotic-resistant bacteria are requested. Currently, bacteriocins/enterocins present a new promising way to overcome this problem, both in prevention and treatment. Therefore, the preventive and medicinal effect of dipeptide enterocin EntA/P was evaluated against MR Staphylococcus epidermidis SEP3/Tr2a strain in a rabbit model, testing their influence on growth performance, glutathione-peroxidase (GPx) enzyme activity, phagocytic activity (PA), secretory (s)IgA, and jejunal morphometry (JM). Eighty-eight rabbits (aged 35 days, meat line M91, both sexes) were divided into experimental groups S (SEP3/Tr2a strain; 1.0 × 105 CFU/mL; dose 500µL/animal/day for 7 days, between days 14 and 21 to simulate the pathogen attack), E (EntA/P; 50 µL/animal/day, 25,600 AU/mL in two intervals, for preventive effect between days 0 and 14; for medicinal effect between days 28 and 42), E + S (EntA/P + SEP3/Tr2a; preventive effect; SEP3/Tr2a + EntA/P; medicinal effect) and control group (C; without additives). Higher body weight was recorded in all experimental groups (p < 0.001) compared to control data. The negative influence/attack of the SEP3Tra2 strain on the intestinal immunity and environment was reflected as decreased GPx activity, worse JM parameters and higher sIgA concentration in infected rabbits. These results suggest the promising preventive use of EntA/P to improve the immunity and growth of rabbits, as well as its therapeutic potential and protective role against staphylococcal infections in rabbit breeding.

Indicators of free radical oxidation in neutrophils of patients with primary and recurrent soft tissue sarcomas

Purpose of the study. To carry out a comparative analysis of lipid peroxidation intensity and antioxidant system indices in blood neutrophils of patients with primary and relapsed soft tissue sarcomas (STS) depending on sex and age.Patients and methods. Of the 81 patients included in the study, 48 had primary STS, 5 patients with continued growth, and 28 patients with recurrent STS. The patients were divided by sex and age; the level of lipid peroxidation products, superoxide dismutase (SOD) activity, total peroxidase activity (TPA), glutathione peroxidase (GPx) activity and reduced glutathione (RG) content in blood neutrophils were investigated by conventional spectrophotometric methods. The comparison groups (donors) consisted of 12 men and 17 women divided into the same age subgroups: ≤ 45 years and &gt; 45 years.Results. In donors, there was a multiple decrease in the SOD/TPA ratio with age, especially in women, and in men this was accompanied by an age-related decrease in GPx activity. In primary sarcomas in older women and in men with continued growth and recurrence, there was an increase in diene conjugates (DC). In men over 45 years of age, there was an increase in SOD, TPA, and GPx. Women of the older age group were characterized by activation of SOD and GPx, expressed in the relapsed process to a significantly greater extent than in men, and an increase in RG was observed in women. The increase in both components of the glutathione system and SOD activity was especially significant in the development of relapses in women for periods exceeding three years, which was accompanied by a decrease in DC content. Conclusions. Neutrophils are characterized by an increase in DC content in patients of both sexes in the older age group with primary and recurrent STS, with its highest content in men with continued growth and relapses. GPx activity, increased in both men and women in the older age group in all variants of STS development, may play an important role in the antioxidant protection of blood cells in STS. Thus, the most pronounced activation of GPx, accompanied by a maximum increase in reduced glutathione and activation of SOD, contributes to a decrease in the level of DC and the absence of MDA increase in women with slow development of recurrences.

Effect of Eight Weeks of Resistance Training on Inflammatory Markers and Antioxidant Indices of Kidney Tissue Following Testosterone Enanthate Abuse in Male Rats

Introduction: Abuse of anabolic steroids can lead to kidney tissue damage.The aim of this study was to investigate the effect of eight weeks of resistance training on inflammatory markers and antioxidant indices of kidney tissue of male rats following testosterone enanthate abuse.&#x0D; Methods: In this experimental study, 24 male Wistar rats were randomly divided into three groups: 1) Control, 2) Training and 3) Training + Testosterone. Rats in groups 2 and 3 performed resistance training five sessions a week for eight weeks. Moreover, group 3 received 20 mg/kg of testosterone enanthate intramuscularly three days a week. The activity levels of Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) were measured by spectrophotometric method and the gene expression of Interleukin-6 (IL-6) and Tumor necrosis factor alpha (TNF-α) were measured by Real Time PCR method. The data were analyzed using SPSS version 16 software. To analyze the findings, To analyze the findings, one-way analysis of variance was used (P≥0.05).&#x0D; Results: Resistance training caused a significant decrease in GPx activity (P=0.001) compared to the control group. IL-6 gene expression in the training + testosterone group was significantly more than the training group (P=0.04) and the control group (P=0.001). Training + testosterone caused a significant increase in TNF-α gene expression compared to the training group (P=0.02) and the control group (P=0.008). Furthermore, the level of GPx activity in the training + testosterone group was significantly lower than the control group (P=0.001), but no significant difference was observed in the training + testosterone groups and the training group (P=0.93).&#x0D; Conclusion: It seems that testosterone abuse along with intense resistance training is associated with a decrease in the function of the antioxidant system and increase in inflammatory markers in the kidney tissue.

Gut microbiota contribution to selenium deficiency-induced gut-liver inflammation.

There is limited knowledge about the factors that drive gut-liver axis changes after selenium (Se) deficiency-induced gut or liver injuries. Thus, we tested Se deficiency in mice to determine its effects on intestinal bacterial balance and whether it induced liver injury. Serum Se concentration, lipopolysaccharide (LPS) level, and liver injury biomarkers were tested using a biochemical method, while pathological changes in the liver and jejunum were observed via hematoxylin and eosin stain, and a fluorescence spectrophotometer was used to evaluate intestinal permeability. Tight junction (TJ)-related and toll-like receptor (TLR) signaling-related pathway genes and proteins were tested using quantitative polymerase chain reaction, western blotting, immunohistochemistry, and 16S ribosomal ribonucleic acid gene-targeted sequencing of jejunum microorganisms. Se deficiency significantly decreased glutathione peroxidase activity and disrupted the intestinal flora, with the most significant effect being a decrease in Lactobacillus reuteri. The expression of TJ-related genes and proteins decreased significantly with increased treatment time, whereas supplementation with Se, fecal microbiota transplantation, or L. reuteri reversed these decreases. Signs of liver injury and LPS content were significantly increased after intestinal flora imbalance or jejunum injury, and the levels of TLR signaling-related genes were significantly increased. The results indicated that Se deficiency disrupted the microbiota balance, decreased the expression of intestinal TJ factors, and increased intestinal permeability. By contrast, LPS increased due to a bacterial imbalance, which may induce inflammatory liver injury via the TLR4 signaling pathway.

Exploring the Relationship between Antioxidant Enzymes, Oxidative Stress Markers, and Clinical Profile in Relapsing-Remitting Multiple Sclerosis.

We aimed to investigate the extent of alterations in the pro/antioxidant balance in the blood of patients with relapsing-remitting multiple sclerosis (RRMS) in relation to drug-modified therapy, gender, disability score, and disease duration. 161 patients (67 men and 94 women, aged 24-69 years, median 43.0) and 29 healthy individuals (9 men and 20 women, aged 25-68 years, median 41.0) were included in the study. We measured the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) as well as the concentration of interleukin-6 (IL-6), lipid peroxidation parameters (LPO), total oxidant status (TOS), and total antioxidant capacity (TAS). The activity of SOD did not show any significant differences between patients with RRMS and the control group in our study. In contrast, significant decreased GPx activity and increased CAT activity was observed in the blood of patients with RRMS compared to the control group. Additionally, the activity of CAT was influenced by gender and the use of disease-modifying therapies. Disease-modifying therapies also affected the concentration of TOS, TAS, and LPO. Our studies indicated that enhancing GPx activity may be more beneficial to providing potential therapeutic strategies aimed at modulating antioxidant defenses to mitigate oxidative stress in this disease.

Chronic Thermal Acclimation Effects on Critical Thermal Maxima (CTmax) and Oxidative Stress Differences in White Epaxial Muscle between Surface and Cave Morphotypes of the Mexican Cavefish (Astyanax mexicanus).

AbstractIn the face of increasing environmental temperatures, operative differences between mitochondrial function and whole-animal phenotypic response to the environment are underrepresented in research, especially in subtemperate ectothermic vertebrates. A novel approach to exploring this connection is to examine model species that are genetically similar but that have different whole-animal phenotypes, each of which inhabits different environments. The blind Mexican cavefish (Astyanax mexicanus) has the following two morphotypes: a surface form found in aboveground rivers and an obligate cave-dwelling form. Each morphotype inhabits vastly different thermal and oxygen environments. Whole-animal and mitochondrial responses to thermal acclimation and oxidative stress, with respect to increasing temperatures, have not been previously determined in either morphotype of this species. Here, we chronically acclimated both morphotypes to three temperatures (14°C, 25°C, and 31°C) to establish potential for acclimation and critical thermal maxima (CTmax) for each morphotype of this species. After measuring CTmax in six cohorts, we additionally measured enzymatic antioxidant capacity (catalase, superoxide dismutase, and glutathione peroxidase activities), peroxyl scavenging capacity, and lipid peroxidation damage in white epaxial muscle for each individual. We found a significant effect of acclimation temperature on CTmax (, ) but no effect of morphotype on CTmax (, ). Additionally, we found that morphotype had a significant effect on glutathione peroxidase activity, with the surface morphotype having increased glutathione peroxidase activity compared with the cave morphotype (, ). No other oxidative stress variable demonstrated significant differences. Increases in CTmax with chronic thermal acclimation to higher temperatures suggests that there is some degree of phenotypic plasticity in this species that nominally occupies thermally stable environments. The decreased glutathione peroxidase activity in the cave morphotype may be related to decreased environmental oxygen concentration and decreased metabolic rate in this environmentally constrained morphotype compared to in its surface-living counterparts.

Physiological and transcriptomic analyses reveal that phytohormone pathways and glutathione metabolism are involved in the arsenite toxicity response in tomatoes

The main forms of inorganic arsenic (As) in soil are arsenate [As(V)] and arsenite [As(III)]. Both forms inhibit plant growth. Here, we investigate the effects of As(III) toxicity on the growth of tomatoes by integrating physiological and transcriptomic analyses. As(III) toxicity induces oxidative damage, inhibits photosynthetic efficiency, and reduces soluble sugar levels. As(III) toxicity leads to reductions in auxin, cytokinin and jasmonic acid contents by 29 %, 39 % and 55 %, respectively, but leads to increases in the ethylene precursor 1-amino-cyclopropane carboxylic acid, abscisic acid and salicylic acid contents in roots, by 116 %, 79 % and 39 %, respectively, thereby altering phytohormone signalling pathways. The total glutathione, reduced glutathione (GSH) and oxidized glutathione (GSSG) contents are reduced by 59 %, 49 % and 94 % in roots; moreover, a high GSH/GSSG ratio is maintained through increased glutathione reductase activity (increased by 214 %) and decreased glutathione peroxidase activity (decreased by 40 %) in the roots of As(III)-treated tomato seedlings. In addition, As(III) toxicity affects the expression of genes related to the endoplasmic reticulum stress response. The altered expression of aquaporins and ABCC transporters changes the level of As(III) accumulation in plants. A set of hub genes involved in modulating As(III) toxicity responses in tomatoes was identified via a weighted gene coexpression network analysis. Taken together, these results elucidate the physiological and molecular regulatory mechanism underlying As(III) toxicity and provide a theoretical basis for selecting and breeding tomato varieties with low As(III) accumulation. Therefore, these findings are expected to be helpful in improving food safety and to developing sustainable agricultural.

Effect of olive oil on oxidative and DNA damage induced by imidacloprid

The current study aimed to evaluate the effect of olive oil (OLO) on the genotoxicity of imidacloprid (IMI). Rats were divided into four groups: control group, IMI group that was exposed to 22.5 mg/kg b.w. IMI for 4, 8, and 12 weeks; OLO before IMI group – dosed orally with OLO at a dose of 10 mL/kg body weight for two weeks before IMI exposure of 4, 8, and 12 weeks. The OLO after IMI group was treated with OLO in a dose of 10 mL /kg body weight for two weeks after IMI exposure of 4, 8, and 12 weeks. Malondialdehyde (MDA) and glutathione peroxidase (GPx) levels were measured in blood serum. The bone marrow from the femur was collected for the comet assay. The results revealed that exposure to IMI induced DNA damage, which was associated with increased blood MDA level and decreased GPx activity. However, treatment with OLO resulted in decreasing both DNA damage and MDA level while increasing GPx activity. On the other hand, pre-treatment with OLO was effective in decreasing the DNA damaging effect of IMI.

Effects of continuous prenatal and postnatal global system for mobile communications electromagnetic waves (GSM-EMW) exposure on the oxidative stress biomarkers in female rat liver

In light of the increased use of communication technologies, the harm caused by continuous exposure to emitted radiation on pregnancy and developing newborns is among the public concerns. Using Sprague-Dawley rats, our study investigates the effects of 24 h/day prenatal and postnatal 900 MHz radiofrequency electromagnetic radiation (RF-EMR) exposure of female rats on liver oxidative stress (OS) and other hepatic parameters at postnatal days (PND) 1, 9, and 21. Our results showed that RF-EMR exposure led to an increase in oxidative stress status as indicated by a significant elevation in MDA level at PND9 and PND21, a decrease in catalase (CAT) activity at all ages, a reduction (PND1 and PND9) in catalase amounts and mRNA expression, in addition to a decrease in GPx activity at PND21 in the exposed group. Current findings also showed a significant increase in cytoSOD at PND9 and 21 and a reduction in mitoSOD at PND21 in the exposed groups compared to the control groups. However, significant increases in glutathione peroxidase (GPx) level and mitoSOD activity were observed at all studied ages. Furthermore, cytoSOD activity showed a significant reduction in PND1, whereas in PND9 the value of this parameter increased compared to the non-exposed group. Moreover, while SOD1 mRNA expression increased at PND1, it decreased at PND9 and 21. However, GPx1 expression was shown to be always decreased in the exposed group. In addition, at PND1 and 9, exposed rats showed a similar response on Akt1, nuclear factor erythroïd 2-related factor 2 (Nrf-2), and intercellular adhesion molecule-1 (ICAM-1) expression. Therefore, an increased oxidative stress status produced from a continuous (24 h/day) GSM-modulated 900 MHz radiofrequency electromagnetic radiation (RF-EMR) exposure during the prenatal and postnatal periods may result in adverse health effects during future life stages.

Biomarkers of Oxidative Stress in the Muscle Tissue of Atlantic Salmon (Salmo salar L.) Treated in vitro by Extracts of Chelidonium majus L.

Consistent with our previous studies, we continue to evaluate the antioxidant potential of representatives belonging to the Papaveraceae family collected from the northern part of Poland using a muscle tissue model of Atlantic salmon (Salmo salar L.). Therefore, in the present study, oxidative stress biomarkers (2-thiobarbituric acid reactive substances (TBARS), aldehydic and ketonic derivatives of oxidatively modified proteins (OMP), total antioxidant capacity (TAC)) and also activity of antioxidant enzymes (catalase, superoxide dismutase, glutathione peroxidase) were used for evaluating the in vitro antioxidant activity of root and stalk extracts derived from great celandine Chelidonium majus L. (CM) collected in urban and rural agglomerations of Kartuzy district (Pomeranian province, northern part of Poland). Freshly collected roots and stalks were washed, weighed, crushed, and homogenized in 0.1 M phosphate buffer (pH 7.4) (in the proportion of 1:19, w/w) at room temperature. Incubation of salmon muscle tissue with extracts derived from both the stalks and roots of CM harvested from rural areas resulted in a decrease in lipid peroxidation. The incubation of salmon muscle tissue with extracts derived from both the stems and roots of CM harvested from rural areas resulted in a decrease in lipid peroxidation. Similarly, the use of extracts derived from the roots of CM collected from urban areas resulted in a decrease in TBARS levels. These results suggest that it can be argued that the presence of secondary plant metabolites in CM extracts protects structures of cell membranes against the damaging effects of free radicals. On the other hand, analysis of levels of protein oxidation after incubation of muscle tissue with CM extracts showed that extracts derived from both roots and stalks of CM harvested from urban areas reduced levels of ketonic derivatives of oxidatively modified proteins. Analyzing the total antioxidant capacity after the incubation with CM extracts under in vitro conditions, we concluded that extracts mainly derived from the stalks of CM harvested from both urban and rural areas effectively increase TAC levels. These results are reflected after analysis of antioxidant enzyme activity, where we observed statistically significant increases in superoxide dismutase and catalase activity. On the other hand, the incubation of CM extracts with muscle tissue resulted in a statistically significant decrease in glutathione peroxidase activity compared to the control samples.

Analysis of the Role of PI3K/Akt Pathway in the Realization of the Normalizing Effect of Low-Molecular-Weight Mimetics of NGF and BDNF on Activity of Prooxidant and Antioxidant Systems in C57BL/6 Mice with Modeled Diabetes.

The effect of low-molecular-weight mimetics of NGF and BDNF (GK-2 and GSB-214 in a dose 0.5 mg/kg, respectively) on malondialdehyde content and activity of an antioxidant defense enzyme glutathione peroxidase was studied in experiments on C57BL/6 mice with streptozotocin-induced diabetes. An increase in the malondialdehyde content indicating enhanced formation of peroxidation products and a decrease of glutathione peroxidase activity in the blood plasma of untreated diabetic animals were revealed. Both studied mimetics were shown to attenuate the severity of these disorders. Since the ability of these compounds to activate the PI3K/Akt signaling pathway was previously demonstrated in vitro on HT-22 cell culture, we studied the effect of LY294002, an inhibitor of this pathway, on the above parameters. It was found that LY294002 attenuates the normalizing effect of GK-2 and GSB-214 only in relation to glutathione peroxidase activity, but not malondialdehyde level.

Association between the physical activity and metabolic syndrome in residents of a foot-hill area in India

Background and aimsPhysical inactivity, has been linked to development of metabolic syndrome, which increases the risk of developing cardiovascular disease. Aim of the study was to assess the level of physical activity and it's association with prevalence of metabolic syndrome and oxidative stress in a semiurban foothill population of India. MethodsParticipants (n = 288), >18 years of age were enrolled. Their demographics and clinical details were recorded. Fasting plasma glucose, triglycerides, cholesterol and erythrocyte glutathione peroxidase activity were measured. Physical activity was estimated using Global Physical Activity Questionnaire and converted into metabolic equivalent in minutes per week. Results37.5% of study population was found to be physically active, with more males (43.3%) being active than females (23.3%). Inactivity was associated with higher triglycerides levels in males and with higher hip circumference, diastolic blood pressure, triglycerides and fasting plasma glucose in females. No significant difference was found in the oxidative stress, indicated by decreased glutathione peroxidase activity, between active and inactive persons or persons with normal and increased body mass index. ConclusionsThere is a high prevalence of physical inactivity in this region of India, with prevalence of physical inactivity higher is females as compared to males. No significant co-relation was found between the levels of activity and components of metabolic syndrome and oxidative stress in the study population.

Nutritional status of selenium in overweight and obesity: A systematic review and meta-analysis

Selenium is an antioxidant and anti-inflammatory nutrient and regulates important physiological processes, such as carbohydrate and lipid metabolism, immune system actions, and adipocyte differentiation. Given these important functions, several studies have assessed the nutritional status of selenium to elucidate whether its homeostasis is impaired by excess adiposity, which in turn could contribute to the adipose tissue dysfunction and metabolic disorders. However, the results of these studies are quite controversial. Therefore, this systematic review and meta-analysis aimed to assess differences in the nutritional status of selenium in overweight/obese individuals. We searched the PubMed, Scopus, Web of Science, Embase, Cochrane Library, and Virtual Health Library databases. Studies comparing overweight or obese individuals with eutrophic individuals and assessing at least one marker of selenium were included. Comprehensive Meta-Analysis software was used to perform meta-analysis. The effect size was calculated using the raw mean difference or the standardized mean difference, with 95% confidence interval and a p-value of <0.05. Heterogeneity was determined using the I2 index. Subgroup analyses were performed according to sex, body mass index (BMI), and selenium content in the soil. The protocol of this review was registered in the Prospective Register of Systematic Reviews (registration number: CRD42020185405). This systematic review included 73 articles, of which 65 articles were eligible for meta-analysis. There were no significant differences between the overweight/obesity and eutrophy groups in terms of dietary intake and plasma/serum levels of selenium and selenoprotein P levels. The activity of plasma/serum glutathione peroxidase was decreased in the overweight/obese children and adolescents; however, the difference was no statistically significant when the enzyme activity was analyzed in erythrocytes and whole blood. In the adult age group, overweight/obese individuals had decreased activity of both glutathione peroxidase isoforms compared to those individuals with eutrophy. In particular, the activity of erythrocyte glutathione peroxidase was significantly decreased in obese individuals and those living in regions with low-to-moderate selenium content in the soil. Regarding selenium excretion markers, overweight/obese individuals had lower levels of selenium in the urine and nails and a trend of higher levels of selenium in the hair than eutrophic individuals. In conditions of excess adiposity, the main alteration in the nutritional status of selenium is a decrease in glutathione peroxidase activity, particularly in adults with obesity. In addition, reduced levels of selenium in urine and nails can be found in overweight or obese individuals.

Evaluation the Silver Graphene Quantum Dot effect when combined with Resveratrol and Radiation in Colorectal Cancer Cells

The main purpose of this study is to evaluate the efficacy of radiotherapy in combined with silver grapheme quantum dot (SQD) and Resveratrol (Res) on HCT-116 colorectal cancer (CRC) cells. Minimally cytotoxic concentration of SQD (50Um) was selected to evaluate the sensitivity to radiation (2Gy of x-Ray radiation) and to combine treatments. The Acridine Orange/Ethidium Bromide staining was used to detect apoptosis. The caspase 3 mRNA expression level was measured by real time PCR method. Also cyclooxygenase 2 (COX-2) protein expression was studied by western blot technique. Likewise, the superoxide dismutase (SOD) and glutathione peroxidase (GPX) enzyme activities and malondialdehyde(MDA) level were determined. In this study after 24 h , triple combined case showed decreased cell viability versus double and single treatments (p&lt;0.05). Also cellular viability were decreased in SQD-Radiation and SQD-Res in compared to related single treatments (p&lt;0.05). The increased apoptotic cells, was observed in the triple combination group in compared to double and single treated cases. Also, triple combination treatment exhibited decreased GPX activity versus single treatments (p&lt;0.05). The triple combined case showed reduced SOD activity than the single radiation and SQD treatments. A remarkable increase in MDA concentration was observed in triple combination versus single treatments. Likewise in double and triple treatments, upregulation of caspase 3 mRNA were observed versus double and single treatments. There was decline in COX-2 protein expression in triple combinatorial case versus to double and single treated cells. These findings propose that SQD can be utilized as effective nano-radiosensitizer for the CRC treatment.

Production performance, egg quality and some blood parameters of heat-stressed laying hens as affected by dietary supplemental Vit B6, Mg and Zn.

ObjectivesThe effects of dietary supplements, including magnesium sulfate (Mg), zinc oxide (Zn) and vitamin B6 (Vit B6), on the performance of laying hens reared under normal (NC) and heat‐stress (HSC) conditions were investigated.Material and MethodsA total of 288 30‐week‐old Lohmann LSL‐Lite laying hens were randomly divided into 48 cages and assigned to receive one of the eight diets with six replicates and six hens per replicate, based on a 2 × 2 × 2 factorial arrangement of treatments. Dietary supplements, including two levels (0 & 600 mg/kg) of Mg, two levels (0 & 30 mg/kg) of Zn and two levels (0 & 8 mg/kg) of Vit B6, in normal and heat‐stress conditions, were tested at 30–40 and 41–45 weeks of age, respectively.ResultsIn the Vit B6 group, a decrease in feed intake (FI), egg production (EP), albumin, Zn, Fe and Mg, and an increase in triglyceride and insulin in HSC were observed, in addition to a decrease in cholesterol and an increase in egg weight (EW) in NC. Supplemental Mg decreased EP, blood triglycerides and copper in NC, and Zn, Fe and Mg in HSC as well. Feeding Zn, increased egg shape index, total protein and copper in addition to yolk index in NC and Fe in HSC. EWs were higher in hens supplemented with Vit B6+Mg in HSC. Increased insulin and decreased glutathione peroxidase activity were detected with the Vit B6+Zn compared to feeding either Vit B6 or Zn in HSC.ConclusionsThe results indicated Vit B6 could improve EWs and suppress blood cholesterol in NC. Zn improved the egg shape index in NC.

In vivo functional and health benefits of a prebiotic soursop whey beverage processed by high-intensity ultrasound: Study with healthy Wistar rats

New technologies for food processing have been used to enhance the beneficial effects of foods. This study aimed to evaluate the effects of a prebiotic soursop whey beverage processed by high-intensity ultrasound (HIUS) on healthy rats. Whey beverages were processed by HIUS (20KHz, 520 W of nominal power, <53 °C, 20.3 W of acoustic power, energy density of 2.9 kJ.cm−3 and 9.5 min to process 100 mL) and high-temperature short-time (HTST, 75 °C for 15 s) before being supplemented to Wistar rats by gavage for 15 days. Antioxidant, antidiabetic, anti-hypertensive, and anticancer activities, lipid peroxidation, bioactive peptides, and microstructure of the beverages were analyzed. In addition, the body mass, food, and water intake, systolic blood pressure, biochemical and oxidative stress parameters were measured. The sonicated beverage induced satiety, decreased glutathione peroxidase activity, total triglycerides, very-low-density lipoprotein cholesterol, and alanine aminotransferase. These findings suggest that ultrasound technology can provide in vivo health and functional benefits.

Association of genetic variants in the GPX1 and GPX4 genes with the activities of glutathione-dependent enzymes, their interaction with smoking and the risk of acute pancreatitis

Genetic factors and tobacco smoke exposure can be associated with an increased risk of acute pancreatitis (AP). The pathogenesis of AP is associated with intensive oxidative stress. Glutathione peroxidase (GPx) is one of many enzymes involved in the neutralization of free radicals. This study aimed to investigate the impact of SNP rs1050450 in the GPX1 gene and rs713041 in the GPX4 gene on the activity of total GPx in a group of AP patients and healthy subjects. It was found that AP can contribute to decreased GPx activity (in plasma and erythrocyte lysate) accompanied by an increased glutathione reductase (GR) activity and decreased glutathione (GSH) concentration in two groups, non-smokers and smokers. A decreased GPx activity in erythrocyte lysate of AP patients compared to healthy subjects was associated with the occurrence of the CC genotype for SNP rs1050450. It was noted an increased GPx activity and decreased GR activity in erythrocytes of non-smoking AP patients with the TT genotype compared to subjects with the CC and TC genotype for SNP rs713041. However, in the group of smoking AP patients with this genotype, GR activity was elevated compared to non-smokers, which was accompanied by increased GSH concentration. These results can indicate that smoking in the course of AP can change the involvement of antioxidants in dependence on the genotype for the examined SNPs. The CC genotype for SNP rs1050450 and the TT genotype for rs713041 increases the risk of AP recurrence, which may be associated with increased MDA concentration.